Nanopartz Magnetic Nanoparticles

Nanopartz Magnetic Nanoparticles are paramagnetic highly monodisperse spherical magnetic nanoparticles manufactured using proprietary methods that result in exact sizes, highly monodisperse shapes, and long shelf lives. Diameters from 2nm up to 300nm with size variances less than 10% at the larger sizes.  The proprietary manufacturing method results in a very dense magnetic lattice resulting in very high magnetic response. Unlike other magnetic nanoparticles, these particles stay suspended and do not aggregate, and yet are extremely magnetic. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Every product is in stock (99%) and is shipped same day. These particles are are available non-covalently capped citrate, and many other adsorbed polymers, as well as a number of covalent options and other in vitro polymers.

 

AM1-200 M1J05 200 Q10

 

Diameter
(nm)
NPS/ml Molarity (pM) Moles Wt. conc
(mg/ml)
Wt. % PPM Size Dispersity
%PDI
Size Accuracy
(+/- nm)
2 1.16E+17 1.90E+08 1.93E-07 2.5 0.25% 2.5 <35% 1
5 7.41E+15 1.20E+07 1.24E-08 2.5 0.25% 2.5 <20% 2
10 9.26E+14 1.50E+06 1.54E-09 2.5 0.25% 2.5 <20% 2
20 1.16E+14 1.90E+05 1.93E-10 2.5 0.25% 2.5 <20% 5
30 3.43E+13 5.70E+04 5.72E-11 2.5 0.25% 2.5 <10% 5
40 1.45E+13 2.40E+04 2.41E-11 2.5 0.25% 2.5 <10% 5
50 7.41E+12 1.20E+04 1.24E-11 2.5 0.25% 2.5 <10% 5
70 2.70E+12 4.50E+03 4.50E-12 2.5 0.25% 2.5 <10% 5
100 9.26E+11 1.50E+03 1.54E-12 2.5 0.25% 2.5 <10% 5
150 2.74E+11 4.60E+02 4.57E-13 2.5 0.25% 2.5 <10% 25
200 1.16E+11 1.90E+02 1.93E-13 2.5 0.25% 2.5 <10% 50
250 5.93E+10 9.90E+01 9.88E-14 2.5 0.25% 2.5 <10% 50
 

 

Gold Nanoparticles PEI
PEI
Gold Nanoparticles PAA
PAA
Gold Nanoparticles PAH
PAH
Gold Nanoparticles PVP
PVP
Gold Nanoparticles Citrate
Citrate
Gold Nanoparticles CTAB
CTAB
Gold Nanoparticles Nanopartz Carboxylic Acid
Nanopartz Carboxylic Acid

 

Functionalized Nanorod Block Invitro

 

Functionalized magnetic nanoparticle with Nanopartz™ covalently bonded polymer

 

 

Loading Range (nm2)
Charge (mV)
Smaller particles Larger particles Smaller particles Larger particles
PEG
3
1
-27
-12
Alkyne
4
2
-25
-25
Amine
4
2
-20
20
Azide
4
2
-25
-25
Biotin
3
1.5
-25
-15
Carboxyl
4
2
-38
-45
Methyl
4
2
-27
-12
Streptavidin
0.01
0.01
-25
-10
Neutravidin
0.01
0.01
-25
-10
GSH (Glutathione)
3
1.5
-25
-15
Protein A
0.04
0.04
-25
-10
Protein G
0.04
0.04
-25
-10
Maleimide
4
2
-27
-12
NHS
3
1.5
-25
-15
Hydroxyl
4
2
-27
-12
Thiol
5
5
-30
-30
Galactose
2
2
-30
-30
Mutab
5
5
30
40
Secondary IgG
0.02
0.02
0.02
0.02

 

MW (g/mole)
Zeta Charge (mV)
Salt resistant?
Concentration Sensitive?
Easy to adsorb proteins?
Easy to thiolate?
Shelf Life at 4C
Citrate
192
-35
1.5mM
Yes
Yes
Yes
6 months
Nanopartz Carboxylic Acid
212
-30
15mM
Less than Citrate
Better than Citrate
Better than Citrate
years
PEI
100K
30
150mM
No
Yes
Yes
6 months
PAA
10K
-30
150mM
No
Yes
Yes
6 months
PAH
10K
20
150mM
No
Yes
Yes
6 months
PVP
40K
-10
0.5M
No
No
Yes
6 months
CTAB
364
35
0.5M
Yes
No
No
6 months
BSA
66K
-15
0.5M
No
No
No
6 months

 

Composition

These spherical magnetic nanoparticles are shipped in  DI water with no measurable residual reactants. These particles are not manufactured in citrate. Citrate and the other ligands are functionalized after manufacturing allowing for a minimal, controlled amount of ligand to be used.

Custom Formulation

Diameters 2 to 300nm.  Other sizes are special order.  Please contact us

Quantity

This product is available in 1mL volumes and larger. For the oligo products, the oligo is limited to 5nmoles. For the smaller sizes of nanoparticles, depending on whether the monovalent option is purchased, the amount of nanoparticles will be limited by the upper limit of the oligo. For example, for the 2nm product, if the monovalent option is purchased, you will received 5nmoles of nanoparticles.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with different adsorbed ligands: our proprietary carboxylic acid, citrate, and with PEI, PAH, PAA, BSA, and PVP polymers.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic unless shipped in CTAB. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for mass measurements

TEM (Phillips CM-100 100KV) for sizing

 

How do you size your magnetic nanoparticles? Does the size include the capping agent?

We use two methods to size our magnetic nanoparticles; TEM, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

 

"We were previously making our own gold nanoparticles using the commonly used technique of citrate reduction of Au(III). The colloids generated are broadly polydisperse, and we were concerned that our application of these particles in immuno-gold labeling of cells for optical coherence microscopy (OCM) would be utilizing only the large diameter tail of the distribution. We purchased the sampler package of NanoPartz gold spheres (30 nm, 50 nm, 70 nm, 90 nm) and examined each size carefully with dynamic light scattering, OCM, and spectrophotometry. Our measurements indicated diameters a couple nanometers larger than specifications (a result of measurements on the fully hydrated state?), and remarkable monodispersity - within specs. It is clear to us now that our early immuno-gold results were dominated by the largest diameter spheres of our early colloids. We are delighted by the NanoPartz monodisperse preparations and recommend them strongly!"

Dr. Richard C. Haskell 
Professor of Physics & Director of Physics Clinic 
Physics Department 
Harvey Mudd College



"The scientists at Nanopartz have developed a line of colloidal gold nanorods with extremely high monodispersity in size, shape, and mass. The surfactant layer is highly stable and readily displaced for biofunctionalization. In every test, in vitro and in vivo, these materials have exceeded our expectations."


Geoffrey A. von Maltzahn
NSF and Whitaker Graduate Fellow
Harvard-MIT Division of Health Sciences and Technology
Laboratory for Multiscale Regenerative Technologies

 

Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres

A Malugin, H Ghandehari - Journal of Applied Toxicology, 2010 - Wiley Online Library
... Plain, PEGylated spherical and PEGylated rod GNPs were obtained from Nanopartz (Salt Lake ...
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. ...
4. TEM images of PC-3 cells incubated with plain spherical gold nanoparticles 30 nm ...

 

Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages

MM Janát-Amsbury, A Ray, CM Peterson… - European Journal of …, 2011 - Elsevier
... PEGylated gold nanoparticles (spherical particles of 50 nm in diameter and rod-shaped particles
with reported dimensions of 10 × 45 nm) were purchased from Nanopartz, Inc. (Loveland, CO,
USA). Before used, gold nanoparticle samples were incubated with cell culture ...

 

A simple millifluidic benchtop reactor system for the high-throughput synthesis and functionalization of gold nanoparticles with different sizes and shapes

SE Lohse, JR Eller, ST Sivapalan, MR Plews… - ACS …, 2013 - ACS Publications
... from several retailers in concentrations of >30 ppm (Sigma-Aldrich, Nanopartz, Nanoseedz, etc.). ...
for AuNRs in 2005 (which uses sodium borohydride, rather than spherical AuNP seeds ... batch
syntheses, even though the reactor permits synthesis of gold nanoparticles at higher ...

 

 

 

The manufacturing method for these nanoparticles are based on our proprietary method. This invention allows the first use of a reducing agent to be used at room temperature. Unlike citrate which is only a reducing agent at the boiling temperature of water - thereby quickly making non-monodisperse magnetic nanoparticles, our reducing agent works by slowing building nanoparticles from seed, atom by atom, over the course of up to hours. The result is a highly monodisperse product with unrivaled spherical shapes.

All of these "bare" particles are capped with ligands that are easily replaced with thiol and charge chemistries. We do not recommend using the carboxyl termination on our proprietary ligand as a conjugatable attachment.

 

  Nanopartz™Magnetic Nanoparticles Other Technologies
Monodispersity High (<10%) Low (>15%)
Size Accuracy High (+/-5nm) Low
Linewidths Narrow Broad
Storage lifetime Years 6 months at 4C
Ability to ligand exchange Easy More difficult
Tendency to Aggregate Very small High

 

  • Highly accurate and monodisperse in size, shape, and batch to batch uniformity.
    • Monodisperse sizes and shapes leads to better standards for electron microscopy and other diagnostics.
  • Accurate to +/- 5 nm in diameter.
    • More accurate sizing makes for better SPR matching for Surface Enhanced Raman Labels.
  • Size monodispersity better than 10% CV for many sizes.
  • Facile conjugations allow for coating of antibodies, receptors, ligands, and DNA.
  • Slightly negatively charged (reduced aggregation).
  • Facile synthesis. 

Example part number is AM1-30-CIT-DIH-2.5-5-CS-EP where:

AM1 - Product family number for Nanopartz Magnetic Nanoparticles

30 - Center Diameter in nanometers. Other choices are 2-300nm

CIT - Capping ligand, in this case Citrate. Other choices are NPC, CTAB, PAA, PVP, PEI, PAH, PSS, BSA

DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS

2.5 - Amount in mg/mL

5 - Volume (mL)

CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification.

EP - Endotoxin Purified - The product is endotoxin purified and certified.

How to Order

Ordering by scrolling down and selecting the options from the selection below.

 

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Nanopartz Magnetic Nanoparticles with Adsorbed Ligands
Nanopartz Magnetic Nanoparticles with Adsorbed Ligands
Part Number: AM1-(Diameter)-(CAPPING AGENT)-(BUFFER)-(Wt)-(VOL)-CS-EP
Example part number for a 30nm diameter magnetic nanoparticle, citrate capping agent, in water, 2.5mg in 1mL, with sterilization certificate, no endotoxin purification would be: AM1-30-CIT-DIH-2.5-1-CS
 Diameter (nm) from 2 to 250nm
 CIT (Citrate)
NPC (Nanopartz Carboxylic Acid
PEI (100K Polyethylenimine)
PAH (25K Polyallylamine hydrochloride)
PAA (10K Polyacrylic acid)
PVP (40K Polyvinylpyrrolidone)
BSA (Bovine Serum Albumin)
CTAB (Cetyltrimethylammonium bromide)
Tannic Acid
SDS
 DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Price: $440.00
CM1 Functionalized Magnetic Nanoparticles
CM1 Functionalized Magnetic Nanoparticles
Product Number: CM1
Options: -(Diameter)-(FUNCTIONALIZATION)-(BUFFER)-(Wt)-(VOL)-CS-EP
Example part number for a 30nm diameter magnetic nanoparticle, Carboxyl polymer, in water, 2.5mg in 1mL, with sterilization certificate, no endotoxin purification would be: CM1-100-TC-DIH-2.5-1-CS
 Diameter (nm) from 2 to 250nm
 Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
 DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Price: $480.00
CASM1 Secondary Antibody Conjugated Magnetic Nanoparticles
CASM1 Secondary Antibody Conjugated Magnetic Nanoparticles
Part Number: CASM1

Options: -(SIZE)-(FLUOROPHORE)-(HOST)-(REACTIVITY)-(POLYMER BRIDGE LENGTH)-(BUFFER)-(WT)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL)-(LYO) Example part number for a 30nm diameter sphere, 550nm fluorophore, Goat anti Human IgG, 2KPEG polymer bridge, in water, 2.5mg in 1mL would be: CASM1-30-550-NPB-Rabbit-AntiHuman-DIH-2.5-1
 Diameter (nm) from 2 to 250nm
 Covalent polymer bridge length - the distance between the gold nanoparticle and the Ab attachment
NPB - Nanopartz Proprietary Bridge is 1-2nm in length)
PEG lengths are shown Here
 DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Negative Control Using the same source gold nanoparticle lot, this product provides a non-reactive negative control 50 OD in either 0.1mL or 1mL volumes. Use this product for confirmation of your active ligand (ie amine, etc.)
0.1NC (0.1mL Negative Control) (+$100.00)
1NC (1mL Negative Control) (+$480.00)
Lyophilized
Lyophilized (+$20.00)
Price: $480.00
CAM1 Primary Antibody Conjugated Magnetic Nanoparticles
CAM1 Primary Antibody Conjugated Magnetic Nanoparticles
Part Number: CAM1

Options: -(SIZE)-(FLUOROPHORE)-(BRIDGE)-(REACTIVITY)-(Ab)-(BUFFER)-(WT)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL)-(LYO) Example part number for a 30nm diameter sphere, 550nm fluorophore, Ab, in water, 2.5mg in 1mL would be: CAM1-30-550-NPB-Chitosan-DIH-2.5-1
 Diameter (nm) from 2 to 250nm
 Covalent polymer bridge length - the distance between the gold nanoparticle and the Ab attachment
NPB - Nanopartz Proprietary Bridge is 1-2nm in length)
PEG lengths are shown Here
 DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Negative Control Using the same source gold nanoparticle lot, this product provides a non-reactive negative control 50 OD in either 0.1mL or 1mL volumes. Use this product for confirmation of your active ligand (ie amine, etc.)
0.1NC (0.1mL Negative Control) (+$100.00)
1NC (1mL Negative Control) (+$480.00)
Lyophilized
Lyophilized (+$20.00)
Price: $0.00
COM1 Custom Oligo Functionalized Magnetic Nanoparticles
COM1 Custom Oligo Functionalized Magnetic Nanoparticles
Product #: COM1
OPTIONS: -(SIZE)-(BRIDGE)-(OLIGO)-(MONO)-(FLUOROPHORE)-(BUFFER)-(WT)-(VOL)-(CS)-(EP)-(NR) Up to 30mer.

Example part number is COM1-30-4C-OLIGO-DIH-2.5-1-CS-EP-NC where:
COM1 - Product family number for Oligo Functionalized Magnetic Nanoparticles.
10 - Sphere diameter in nanometers. Other choices are 2 to 250nm
4C- Carbon length, other options are 10C and 20C
OLIGO - Please enter Oligo sequence from 5' to 3', up to 30 mer
MONOVALENT OPTION - Single oligo per nanoparticle
FLUOROPHORE OPTION - Choose from 550 to 800nm
DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS
2.5 - Weight.
1 - Volume (mL). Other choices are 5mL, 10mL and more
CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification
EP - Endotoxin Purified - The product is endotoxin purified and certified
Negative Control - Using the same source lot of magnetic nanoparticles and covalent polymer, a non reactive terminal group is added
 Diameter (nm) from 2 to 250nm
 Covalent polymer bridge length - the distance between the gold nanoparticle and the 5' oligo end
Optional Monovalent One active ligand group per nanoparticle
Mono (+$40.00)
 Additional Fluorophore tag from 400nm to 800nm
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Negative Control Using the same source gold nanoparticle lot, this product provides a non-reactive negative control 50 OD in either 0.1mL or 1mL volumes. Use this product for confirmation of your active ligand (ie amine, etc.)
0.1NC (0.1mL Negative Control) (+$100.00)
1NC (1mL Negative Control) (+$480.00)
Price: $680.00