Nanopartz™ Accurate™ Spherical Gold Nanoparticles for Intrinsic Raman Scattering

Nanopartz™ Accurate™ Spherical Gold Nanoparticles are spherical gold nanoparticles manufactured using patented, patent pending, and proprietary methods that result in exact sizes, highly monodisperse sizes and shapes, long shelf lives, along with significantly improved reactivity. Diameters from 1.8nm to 125nm with size accuracies up to 0.1nm and size variances less than 4% at sizes larger than 40nm. Peak SPRs range from 505nm to 570nm. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data.

Utilizing its natural negative charge, high k-value for binding, these particles are perfect for enhancing the Raman signal of added analytes. This product comes highly concentrated in 1mL vials, ready for dilution in water.

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Accurate Concentrated SGNPs  1.8-150nm
Accurate Concentrated SGNPs 1.8-150nm
Part# AC11-(SIZE)-(LIGAND)-(VOL)-DI Water
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Price: $400.00
Accurate SGNPs 10-90nm 1mL KIT
Accurate SGNPs 10-90nm 1mL KIT
Part# AK11-(SIZE)-1mL; Minimum Qty. 8, 1mL ea. in 18MEG DI Water; Enter Qty. for each size. Only for online orders.
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
Price: $0.00

 

 

Part #
Diam (nm)
Peak SPR (nm)
Optical Density (1cm)
NPS/ml
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Notes
A11-1.8
1.8
505-508
1
8.38E+14
50
0.01%
50
1.40E+06
7.16E+05
7.16E+05
0.00E+00
<35%
1,3
A11-2.2
2.2
505-508
1
4.59E+14
50
0.01%
50
7.65E+05
1.31E+06
1.31E+06
0.00E+00
<25%
1,3
A11-3
3
505-508
1
1.81E+14
50
0.01%
50
3.02E+05
3.32E+06
3.32E+06
0.00E+00
<20%
1,3
A11-4
4
505-508
1
7.64E+13
50
0.01%
50
1.27E+05
7.86E+06
7.86E+06
0.00E+00
<20%
1,3
A11-5
5
505-508
1
3.91E+13
50
0.01%
50
6.52E+04
1.53E+07
1.53E+07
0.00E+00
<20%
2,3
A11-10
10
514-520
1
4.89E+12
50
0.01%
50
8.14E+03
1.23E+08
1.23E+08
0.00E+00
<15%
2,3
A11-15
15
514-520
1
1.45E+12
50
0.01%
50
2.41E+03
4.14E+08
4.14E+08
0.00E+00
<15%
2,3
A11-20
20
514-520
1
6.11E+11
50
0.01%
50
1.02E+03
9.82E+08
9.82E+08
0.00E+00
<10%
2,3
A11-25
25
514-520
1
3.13E+11
50
0.01%
50
521
1.92E+09
1.92E+09
0.00E+00
<10%
2
A11-30
30
523
1
1.81E+11
50
0.01%
50
302
3.32E+09
3.32E+09
0.00E+00
<6%
2
A11-35
35
526
1
1.14E+11
50
0.01%
50
190
5.26E+09
5.26E+09
0.00E+00
<6%
2
A11-40
40
527
1
7.64E+10
50
0.01%
50
127
7.86E+09
7.84E+09
2.28E+07
<4%
2
A11-45
45
529
1
5.36E+10
50
0.01%
50
89.4
1.12E+10
1.10E+10
2.06E+08
<4%
2
A11-50
50
531
1
3.91E+10
50
0.01%
50
65.2
1.53E+10
1.48E+10
5.97E+08
<4%
2
A11-55
55
533
1
2.94E+10
50
0.01%
50
49
2.04E+10
1.91E+10
1.32E+09
<4%
2
A11-60
60
536
1
2.26E+10
50
0.01%
50
37.7
2.65E+10
2.40E+10
2.52E+09
<4%
2
A11-65
65
539
1
1.78E+10
50
0.01%
50
29.7
3.37E+10
2.93E+10
4.40E+09
<4%
2
A11-70
70
542
1
1.42E+10
50
0.01%
50
23.7
4.21E+10
3.49E+10
7.20E+09
<4%
2
A11-75
75
545
1
1.16E+10
50
0.01%
50
19.3
5.18E+10
4.06E+10
1.12E+10
<4%
2
A11-80
80
549
1
9.54E+09
50
0.01%
50
15.9
6.29E+10
4.61E+10
1.68E+10
<4%
2
A11-85
85
553
1
7.96E+09
50
0.01%
50
13.3
7.54E+10
5.11E+10
2.43E+10
<4%
2
A11-90
90
558
1
6.70E+09
50
0.01%
50
11.2
8.95E+10
5.52E+10
3.43E+10
<4%
2
A11-95
95
563
1
5.70E+09
50
0.01%
50
9.5
1.05E+11
5.81E+10
4.72E+10
<4%
2
A11-100
100
569
1
4.89E+09
50
0.01%
50
8.1
1.23E+11
5.91E+10
6.37E+10
<4%
2
A11-125
125
600
1
2.50E+09
50
0.01%
50
4.2
2.40E+11
1.29E+10
2.27E+11
<4%
2
A11-150
150
612
1
1.45E+09
50
0.01%
50
2.4
4.14E+11
-2.07E+11
6.21E+11
<4%
2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >= 1 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

 

 

Part #
Diameter (nm)
Peak SPR (nm)
Optical Density (1cm)
NPS/ml
Wt. conc (mg/ml)
Wt. %
PPM
Molarity (pM)
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Notes
AC11-1.8
1.8
505-508
100
8.38E+16
5
0.50%
5000
1.40E+08
7.16E+05
7.16E+05
0.00E+00
<35%
1,3
AC11-2.2
2.2
505-508
100
4.59E+16
5
0.50%
5000
7.65E+07
1.31E+06
1.31E+06
0.00E+00
<25%
1,3
AC11-3
3
505-508
100
1.81E+16
5
0.50%
5000
3.02E+07
3.32E+06
3.32E+06
0.00E+00
<20%
1,3
AC11-4
4
505-508
100
7.64E+15
5
0.50%
5000
1.27E+07
7.86E+06
7.86E+06
0.00E+00
<20%
1,3
AC11-5
5
505-508
100
3.91E+15
5
0.50%
5000
6.52E+06
1.53E+07
1.53E+07
0.00E+00
<20%
2,3
AC11-10
10
514-520
100
4.89E+14
5
0.50%
5000
8.14E+05
1.23E+08
1.23E+08
0.00E+00
<15%
2,3
AC11-15
15
514-520
100
1.45E+14
5
0.50%
5000
2.41E+05
4.14E+08
4.14E+08
0.00E+00
<15%
2,3
AC11-20
20
514-520
100
6.11E+13
5
0.50%
5000
1.02E+05
9.82E+08
9.82E+08
0.00E+00
<10%
2,3
AC11-25
25
514-520
100
3.13E+13
5
0.50%
5000
5.21E+04
1.92E+09
1.92E+09
0.00E+00
<10%
2
AC11-30
30
523
100
1.81E+13
5
0.50%
5000
3.02E+04
3.32E+09
3.32E+09
0.00E+00
<6%
2
AC11-35
35
526
100
1.14E+13
5
0.50%
5000
1.90E+04
5.26E+09
5.26E+09
0.00E+00
<6%
2
AC11-40
40
527
100
7.64E+12
5
0.50%
5000
1.27E+04
7.86E+09
7.84E+09
2.28E+07
<4%
2
AC11-45
45
529
100
5.36E+12
5
0.50%
5000
8.94E+03
1.12E+10
1.10E+10
2.06E+08
<4%
2
AC11-50
50
531
100
3.91E+12
5
0.50%
5000
6.52E+03
1.53E+10
1.48E+10
5.97E+08
<4%
2
AC11-55
55
533
100
2.94E+12
5
0.50%
5000
4.90E+03
2.04E+10
1.91E+10
1.32E+09
<4%
2
AC11-60
60
536
100
2.26E+12
5
0.50%
5000
3.77E+03
2.65E+10
2.40E+10
2.52E+09
<4%
2
AC11-65
65
539
100
1.78E+12
5
0.50%
5000
2.97E+03
3.37E+10
2.93E+10
4.40E+09
<4%
2
AC11-70
70
542
100
1.42E+12
5
0.50%
5000
2.37E+03
4.21E+10
3.49E+10
7.20E+09
<4%
2
AC11-75
75
545
100
1.16E+12
5
0.50%
5000
1.93E+03
5.18E+10
4.06E+10
1.12E+10
<4%
2
AC11-80
80
549
100
9.54E+11
5
0.50%
5000
1.59E+03
6.29E+10
4.61E+10
1.68E+10
<4%
2
AC11-85
85
553
100
7.96E+11
5
0.50%
5000
1.33E+03
7.54E+10
5.11E+10
2.43E+10
<4%
2
AC11-90
90
558
100
6.70E+11
5
0.50%
5000
1.12E+03
8.95E+10
5.52E+10
3.43E+10
<4%
2
AC11-95
95
563
100
5.70E+11
5
0.50%
5000
949.9
1.05E+11
5.81E+10
4.72E+10
<4%
2
AC11-100
100
569
100
4.89E+11
5
0.50%
5000
814.5
1.23E+11
5.91E+10
6.37E+10
<4%
2
AC11-125
125
600
100
2.50E+11
5
0.50%
5000
417
2.40E+11
1.29E+10
2.27E+11
<4%
2
AC11-150
150
612
100
1.45E+11
5
0.50%
5000
241.3
4.14E+11
-2.07E+11
6.21E+11
<4%
2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >= 1 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Nanopartz carboxylic acid capped gold nanoparticle

Proprietary Carboxylic Acid (<300Da)

 

 

 

SGNPS

Citrate

 

 

 

 

 

Composition

These bare spherical gold nanoparticles are shipped in  DI water with no measurable residual chemicals. Two ionically adsorbed ligands are available, our proprietary carboxylic acid, and citrate. Both strongly negatively charged for absorption to analytes.

Custom Formulation

Diameters 1.8nm to 150nm.  Other sizes are special order.  Please contact us.

Quantity

This product is available in 1mL and larger.

Introductory Kits

For smaller evaluation volumes, two different kits are available, one with 25mL volume with a four bottle minimum and one with 1mL with an 8 bottle minimum.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product. For our dried particles, storage is room temperature with a shelf life of 1 year.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

The manufacturing method for these nanoparticles are based on our patented method. This invention allowed the first use of a reducing agent to be used at room temperature. Unlike citrate which is only a reducing agent at the boiling temperature of water - thereby quickly making non-monodisperse gold nanoparticles, our reducing agent works by slowing building nanoparticles from seed, atom by atom, over the course of up to twelve days. The result is a highly monodisperse product with unrivaled spherical shapes.

We exclusively licensed this technology in 2006 and have since improved the ability to ligand exchange our nanoparticles with citrate and a proprietary postively charged ligand. Citrate offers the customer a known ligand, but with the benefit of a highly monodisperse product.

  Nanopartz™Accurate™ Spherical Gold Nanoparticles Other Technologies
Monodispersity High (<2%) Low (>15%)
Size Accuracy High (+/-2nm) Low
Linewidths Narrow Broad
Storage lifetime Years 6 months at 4C
Ability to ligand exchange Easy More difficult

 

  • Highly accurate and monodisperse in size, shape, and batch to batch uniformity.
  • Accurate to +/- 2 nm in diameter.
  • Size monodispersity better than 4% CV for many sizes.
  • Greater than 99.9% spheres.
  • Carboxylic acid capping agent is reduced to < 0.1% without adverse effects to the spheres.
  • Extremely stable.  Shelf life is greater than one year.  May be stored at room temperature for extended periods.
  • The light scattering power of a 60 nm gold particle is equivalent to ~5 X 105 fluorescein molecules.
  • Accurate™ SGNPs do not photobleach.
  • Light scatter can be detected by the unaided eye at particle concentrations as low as 10-15 M (femtomolar) in suspension and as low as 0.005 particles/m2 on a transparent surface.
  • Individual gold nanoparticles can be seen by eye in an inexpensive student microscope with simple dark field illumination allowing for very sensitive and inexpensive particle counting assays.
  • Different sized particles scatter different colors of light and therefore can be used as multicolor multiplexed labels.
  • Facile conjugations allow for coating of antibodies, receptors, ligands, DNA without affecting their light scattering properties.
  • Slightly negatively charged (reduced aggregation).
  • Facile synthesis.  Gold chemistry is well known.
  • No known cytotoxicity.  Gold is FDA approved for rheumatoid arthritis.
  • Monodisperse shapes and sizes in production volumes satisfies high volume applications.
  • Smaller negative charge reduces aggregation.
  • Reduced number of additives improves binding to antibodies, receptors, ligands and DNA.
  • Monodisperse sizes and shapes leads to better standards for electron microscopy.
  • No photobleaching means better diagnostic labels with the ability to archive.
  • 105 better scattering power than fluorescein means lower limits of detection with simpler instrumentation.
  • More accurate sizing makes for better SPR matching for Surface Enhanced Raman Labels.
  • Monodisperse sizes and shapes leads to better Resonance Light Scattering labels in sensitivity and multiplexing.

 

Why are your spherical gold nanoparticles so much more monodisperse than conventional citrate stabilized gold nanoparticles?

Citrate stabilized gold nanoparticles are manufactured by combining sodium citrate and hydrogen tetrachloroaurate (gold) in water. In this combination, citrate not only acts as the capping agent, it also reduces the gold to a charge state that allows nanoparticles to form. However, citrate is only a reducing agent at the boiling point of water - so the solution must be boiled. Nanoparticle monodispersity is dependent on an even, homogeneous concentration of the chemical components in solution, and because boiling introduces heterogeneity into the process, the result is a very heterogeneous product.

Our capping agent is similar to citrate and is added to the hydrogen tetrachloroaurate in water like the citrate process. However our capping agent is a reducing agent at room temperature. Consequently, by slowly adding gold over time, we can control the growth, shape, and monodispersity of the gold nanoparticles to within 2nm accuracies.

Why are your citrate stabilized gold nanoparticles so much more monodisperse than conventional gold nanoparticles?

We use our capping agent to manufacture the highly monodisperse gold nanoparticles and then ligand exchange our capping agent with citrate. Since the binding energy (k) for citrate is so much stronger than our capping agent, our capping agent is readily replaced.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

Many companies claim better than 10% PDI using citrate. Is this possible?

We find that hard to believe.

Why is your shelf life time so much longer for your proprietary gold nanoparticles when compared to citrate stabilized gold nanoparticles?

As opposed to citrate, our capping agent is not a growth medium.

Are your proprietary gold nanoparticles interchangeable with citrate stabilized gold nanoparticles?

Yes. However, the user must be aware that in order to stabilize citrate gold nanoparticles, previous manufacturers used to add many stabilizing agents. Consequently for example to ionically attach ligands to gold nanoparticles required the chemist to "drive" the reaction by adding 10-1000 times greater concentrations of the ligand. This is no longer necessary with our gold nanoparticles.

 

"We were previously making our own gold nanoparticles using the commonly used technique of citrate reduction of Au(III). The colloids generated are broadly polydisperse, and we were concerned that our application of these particles in immuno-gold labeling of cells for optical coherence microscopy (OCM) would be utilizing only the large diameter tail of the distribution. We purchased the sampler package of NanoPartz gold spheres (30 nm, 50 nm, 70 nm, 90 nm) and examined each size carefully with dynamic light scattering, OCM, and spectrophotometry. Our measurements indicated diameters a couple nanometers larger than specifications (a result of measurements on the fully hydrated state?), and remarkable monodispersity - within specs. It is clear to us now that our early immuno-gold results were dominated by the largest diameter spheres of our early colloids. We are delighted by the NanoPartz monodisperse preparations and recommend them strongly!"

Dr. Richard C. Haskell 
Professor of Physics & Director of Physics Clinic 
Physics Department 
Harvey Mudd College



"The scientists at Nanopartz have developed a line of colloidal gold nanorods with extremely high monodispersity in size, shape, and mass. The surfactant layer is highly stable and readily displaced for biofunctionalization. In every test, in vitro and in vivo, these materials have exceeded our expectations."


Geoffrey A. von Maltzahn
NSF and Whitaker Graduate Fellow
Harvard-MIT Division of Health Sciences and Technology
Laboratory for Multiscale Regenerative Technologies

 

[HTML] Detection of Adhesion Molecules on Inflamed Macrophages at Early-Stage Using SERS Probe Gold Nanorods

D Pissuwan, Y Hattori - Nano-Micro Letters, 2017 - Springer
... GNRs (1000 µL) obtained from Nanopartz were mixed with 10 mM 4-Mercaptobenzoic acid (4MBA,
10 µL ... In general, five sets of cells for each treatment were used in an experiment. Therefore, all
SERS spectra of each treatment were collected from 15 to 20 measurement areas ...

 

Investigation of degradation and penetration behaviors of dimethoate on and in spinach leaves using in situ SERS and LC-MS

R Hou, M Tong, W Gao, L Wang, T Yang, L He - Food Chemistry, 2017 - Elsevier
... Other users also viewed these articles. Opens document download manager. Do not show again. ...
AuNPs, citrate-capped, 50 nm at 50 μg mL -1 ) were purchased from Nanopartz (Loveland, CO ...
Water used for SERS and LC–MS was produced directly in the laboratory with a Milli ...

 

Method for producing a nanoporous layer on a substrate

B Wolfrum, A Yakushenko, A Offenhaeusser… - US Patent …, 2017 - Google Patents
Sign in. Screen reader users: click this ... 145/165/250±10 nm, and a protective layer made of alkaline
amine (purchased from http://www.nanopartz.com/ (part ... Such an anisotropic nanoporous layer
can be used in surface-enhanced Raman scattering (SERS), as is known from the ...

 

Detection of the tuberculosis antigenic marker mannose-capped lipoarabinomannan in pretreated serum by surface-enhanced Raman scattering

AC Crawford, LB Laurentius, TS Mulvihill, JH Granger… - Analyst, 2017 - pubs.rsc.org
... These relate, in large part, to designing an assay in which the response from SERS is reproducibly
managed. ... First, an aqueous suspension of 60 nm AuNPs (NanoPartz) in 2.0 mM borate buffer
(BB, pH 8.5, Thermo Fisher Scientific) was mixed with 1.0 mM DSNB in acetonitrile ...

 

Bacterial biofilm elimination using gold nanorod localised surface plasmon resonance generated heat

M Pihl, E Bruzell, M Andersson - Materials Science and Engineering: C, 2017 - Elsevier
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for 24 h and then the glass slides were immersed in a gold nanorod solution (10 × 45 nm, citrate
capped, surface plasmon resonance peak 850 nm, Nanopartz) containing 1.5 M ...

 

Signatures of strong coupling on nanoparticles: Revealing absorption anticrossing by tuning the dielectric environment

F Stete, W Koopman, M Bargheer - arXiv preprint arXiv:1701.02907, 2017 - arxiv.org
... This process is well-known and is often em- ployed to obtain single molecule spectra, eg by
SERS [1]. Thus a considerable fraction of the incoming light is transferred to and dissipated
by the molecular shell. ... 25- 600, ligand citrate) came from Nanopartz...

 

[HTML] Photoacoustic flow cytometry for nanomaterial research

DA Nedosekin, T Fahmi, ZA Nima, J Nolan, C Cai… - Photoacoustics, 2017 - Elsevier
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with antibody (against EpCAM receptor) and folic acid (against folate receptor), and covered
with a nonreactive polymer (nonreactive control) were purchased from Nanopartz Inc. ...

 

Plasmonic imaging of surface electrochemical reactions of single gold nanowires

Y Wang, X Shan, H Wang, S Wang… - Journal of the American …, 2017 - ACS Publications
... A three-electrode electrochemical cell with AuNWs (Nanopartz, 75 nm in cross-section diameter,
1 to 10 μm in length) deposited on a graphene/Cytop/Au working electrode (a Ag/AgCl as
reference electrode and a Pt coil as the counter electrode) is mounted on a high numerical ...

 

Plasmon-Enhanced Resonant Excitation and Demethylation of Methylene Blue

TE Tesema, B Kafle, MG Tadesse… - The Journal of Physical …, 2017 - ACS Publications
... visible light induces chemical transformation of MB as it is revealed in the dramatic temporal
evolution of the SERS spectra. ... An aqueous solution of gold nanorods (AuNRs) with
cetyltrimethylammonium bromide (CTAB) stabilizing surface ligand was obtained from Nanopartz...

 

Investigation of Pesticide Penetration and Persistence on Harvested and Live Basil Leaves Using Surface-Enhanced Raman Scattering Mapping

T Yang, B Zhao, AJ Kinchla, JM Clark… - Journal of Agricultural …, 2017 - ACS Publications
... Citrate-capped AuNP colloids (50 nm) were purchased from NANO PARTZ Inc. ... Determination
of Characteristic SERS Signals from Pesticides Placed onto Basil Leaf Surfaces To study the
penetration behaviors of pesticides, it is important to determine the characteristic peaks of ...