Photothermal imaging of passively targeted Nanopartz Ntracker nanorods to MDA-MB-435 tumors 72 hours after IV administration. (Nanopartz/MIT collaboration results)
Photothermal imaging of passively targeted Nanopartz Ntracker nanorods to soft tissue sarcoma tumors in canines 72 hours after IV administration. 10W 808nm laser was used for heating. (Nanopartz Clinical Trials)
Image of Nanopartz GNR labeled cell subsequently labeled with the live cell dye Calcein-AM, shown in red. From Motamedi 2011 BOE (2)5. NCBI Copyright Approval.
Two Photon Image of Nanopartz GNR labeled cells using an incident power of 0.7mW overlayed with corresponding transmitted light micrograph. From Motmaedi 2011 BOE(2)5. NCBI Copyright Approved.
Two photon image using low incident power of 1mW following intravenous injection of Nanopartz gold nanorods in Hamster Model showing blood vessels in the tissue. From Motamedi 2011 BOE (2) 5. NCBI Copyright Approved.
White light microscopy images of control tumor and GNS and Nanopartz Ntracker GNR injected mice. (Pink: cytoplasm stained with eosin, Dark Blue: nuclei stained with hematoxylin). From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
TPIP images of Tumor model (Green: cytoplasm stained with eosin, Yellow: GNSs and Nanopartz Ntracker gold nanorods). From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
TPIP images of Nanopartz gold nanorod injected mice with nuclei staning (YOYO-1 iodide). Two photon IP image with FOV of 720x490um measured at the distance of approximately 100um inside the tumor edge. (Green: nuclei, Yellow: Nanopartz Ntracker gold nanorods). From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
Two Photon IP images of Nanopartz gold nanorod injected mice with blood vessel staining (CD31 immunofluorescence staining). TPIP image with FOV of 790x520um. Red: blood vessels, Yellow: Nanopartz Ntracker Gold Nanorods. From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
Two Photon IP images of liver of Nanopartz Ntracker gold nanorod injected mice. From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
In vivo imaging of single gold nanorods in mice ear blood vessels. Overlay of transmission image (light blue) and a single frame TPL image. Two single nanorods (red spots) are superimposed by a linescan (white). From Wei Nanomedicine (Lond). 2007 February 2(1) 125-132. NCBI Copyright Approval.
B Mode and photoacoustic images of subcutaneous tumor before and 2 minutes after a 200uL bolus of Nanopartz gold nanorods. Photoacoustic contrast in enhanced by gold nanorods in vasculature. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Ex vivo 3D photoacoustic images taken at 750nm of the excised spleen of a mouse 1 day after a tail vein injection of 200uL of Nanopartz gold nanorods and a control mouse spleen. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Ex vivo 3D photoacoustic images taken at 750nm of the excised liver of a mouse 1 day after a tail vein injection of 200uL of Nanopartz gold nanorods and a control mouse liver. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Ex vivo 3D photoacoustic images taken at 970nm of the excised liver of a mouse 1 day after a tail vein injection of 200uL of Nanopartz gold nanorods and a control mouse liver. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Gold nanorod contrast enhanced Optical Coherence Tomography images of a human breast carcinoma tissue sample. The sturcutral OCT image is presented in red, and the SOCT computed GNR density is presented in green.
Nanopartz™in vivo Gold Nanoparticles
Nanopartz™ gold nanoparticles for in vivo application include both the Ntracker™ and Ntherapy™ family of nanorods, along with customer defined oligo and antibody conjugated nanrods for in vivo applications. The Ntracker™ non reactive methyl terminated nanorods are coated in a proprietary dense layer of hydrophilic polymers that shield the gold surface and give the particles ultra-long circulation times. Ntherapy™ nanorods come with similar hydrophilic polymers, but with terminal amine and carboxyl active groups, along with a number of custom popular in vivo targeting ligands. Ntherapy™ also provides an opportunity for the customer to define their own targeting ligands utilizing Nanopartz™ nanorod and polymer technologies. In this family, Nanopartz™ has the ability to covalently conjugate to specific antibody locations, thereby for example, leaving necessary primary amines exposed for optimum activity. The combination of the highly monodisperse Nanopartz™ gold nanorods with the proprietary Nanopartz™ polymers increase circulation times 50% longer than other commercial polymers thereby significantly improving targeting. As opposed to other commercially available nanoparticles such as quantum dots, Nanopartz™ in vivo line of nanorods are completely non-toxic. Whereas Ntracker™ takes advantage of the Enhanced Permeability and Retention effect (EPR) for passive in vivo targeting, Ntherapy™ utilizes popular ligands for active targeting. This product line comes in a diameter of 10nm with SPRs matching all of the popular Near IR CW laser wavelengths; 780, 808, 850, 980, and 1064nm. Ntherapy™ offers options for oligo and customer detailed conjugations. Every batch is radiation sterilized in PBS, and comes with instructions for in vivomouse iv injection. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Every product is in stock (99%) and is shipped next day for many ligands. This product comes in two concentrations, regular 1.75mg/mL, and highly concentrated 10mg/mL.
Nanopartz™ In Vivo Plasmonic Fluorophores™ are fluorophore decorated gold nanorods manufactured using multiple patent pending and proprietary methods that result in fluorophores that exhibit much higher intensities than conventional fluorophores, no photobleaching even under the highest laser intensities, with long circulation times in vivo. Peak fluorescence range from 780 to 1064nm utilizing the same nanorods and in vivo polymer used in our Ntracker and Ntherapy products.
|Part #||Diameter (nm)||Peak SPR Wavelength (nm)||Optical Density (1cm)||NPS/ml||Wt. conc (mg/ml)||Wt. %||PPM||Molarity (pM)||Molar Ext. (M-1cm-1)||Absorption Molar Ext. (M-1cm-1)||Scattering Molar Ext. (M-1cm-1)||Size Dispersity %PDI||Notes|
|Size accuracy +/- 0.1 nm|
|Capping agent is covalently bonded carboxyl|
|Size accuracy +/- 2 nm|
|Peak SPR does not determine size for d<20 nm|
|Vol is 1mL|
|%PDI = Std Dev/Size|
|See Tech Note TN801 for definition of terms and method of analysis|
|All specs typical. May vary batch to batch. Exact values are measured for each batch|
|OD >=50 for all sizes|
|Shape monodispersity (% spheres) > 99.9%|
|Solution is 18MEG DI water|
|Residual Chemicals < 0.1%|
|Part #||Diameter (nm)||Length (nm)||Peak SPR Wave (nm)||Aspect Ratio||OD SPR (AU)||Peak LSPR Wave (nm)||OD LSPR (AU)||Nanorod Vol (nm3)||Nanorods /mL||Wt. conc (mg/ml)||Wt. %||PPM||Molarity (nM)||SPR Molar Ext. (M-1cm-1)||LSPR Molar Ext. (M-1cm-1)||Peak SPR accuracy (nm)||SPR Linewidth 80% (nm)|
|Part #||Diam (nm)||Long Size (nm)||Peak SPR Wave (nm)||Ex/Em (nm)||Wt. conc (mg/ml) (10-3)||Wt. %||PPM||Molarity (pM)||Spectrally Similar Dyes|
|C16-10-780||10||38||780||754/776||1750.0||0.1750%||1750||66.4||Alexa Fluor 750|
|C16-25-750||25||86||750||754/776||2500.0||0.2500%||2500||7.0||Alexa Fluor 750|
|C16-25-680||25||68||680||692/712||2500.0||0.2500%||2500||9.9||Alexa Fluor 680, Cy5.5|
|C16-25-650||25||60||650||652/672||2500.0||0.2500%||2500||12.0||Alexa Fluor 647, Cy5|
|C16-25-633||25||56||633||638/658||2500.0||0.2500%||2500||13.6||Alexa Fluor 633|
|C16-25-600||25||46||594||593/618||2500.0||0.2500%||2500||19.9||Alexa Fluor 594, Texas Red|
|C16-25-550||25||33||546||562/576||2500.0||0.2500%||2500||45.6||Alexa Fluor 546, Alexa Fluor 555|
Functionalized gold nanoparticle with Nanopartz™ Ntherapy covalently bonded polymer. Thickness of polymer is less than the size variablity of the gold nanoparticle.
Conjugations (Ordering Abbreviation)
|Fluorophore Type||Excitation (nm)||Emission (nm)||Spectrally Similar Dyes|
|750||750||773||Alexa Fluor 750|
|680||673||707||Alexa Fluor 680, Cy5.5|
|650||646||662||Alexa Fluor 647, Cy5|
|633||638||658||Alexa Fluor 633|
|600||591||604||Alexa Fluor 594, Texas Red|
|550||555||570||Alexa Fluor 546, Alexa Fluor 555|
|488||494||518||FITC, Alexa Fluor 488|
These polymer coated gold nanorods are shipped in PBS with no measurable residual chemicals. This product is radiation sterilized and is ready for injection.
Popular CW laser wavelengths are available (780, 808, 850, 980, and 1064nm). Other sizes are special order. Please contact us.
This product is available in 1mL and larger but in two different concentrations. The lower concentration is suited for mice, while the higher concentration is suitable for companion animals. For mice, the 1mL injection is suited for 5 injections. For orders larger than 1L, or for orders amounting over 10L per annum, please contact sales for quantity pricing.
Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.
This product comes coated with a proprietary polymer to increase circulation times. Ntracker™ contains a non-reactive methyl termination. Ntherapy™ contains many choices for reactive terminations. Many popular in vivo conjugations are available.
Unfortunately there are no kits available for this product at this time.
Shelf Life/Storage Temperature
This product is guaranteed for one year and may be stored at room temperature.
These products are non cytotoxic.
This product is sterilized.
This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis that includes the following information. We use NIST traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
Ntracker™ is based on extensive research in laboratory, preclinical, toxicology, and more recently clinical trials. The technology is based on patented, patent pending, and proprietary methods. Nanopartz™ has the capacity for up to 1kg of Ntracker™product per day in its audited ISO 9000 facility. Ntherapy™ offers the same technology as Ntracker™ with the added advantage of popular in vivo conjugations and customer specified oligo and antibody choices.
This product has been extensively tested in mice and companion animals.
Photoacoustic is provided by our partner:
VisualSonics is the world leader in real-time, in vivo, high-resolution, micro-imaging systems, providing modalities specifically designed for preclinical research. VisualSonics has commercialized Vevo LAZR Technology, a state-of-the-art photoacoustic imaging system with inherent coregistration and capabilities for imaging gold nanorods in vivo. "Listen to the Light" - Photoacoustic imaging with the new Vevo LAZR System.
Development and optimization of near-IR contrast agents for immune cell tracking
- Covalent bonds insure specificity, stability, long shelf life
- Buffer Stability - stable from pH 4-9
- No sodium azide
- No BSA
- Polymer coating insures no aggregation in high salts, reduced nonspecific binding
- Well Characterized
- Customer can select buffer
- Customer can select gold nanoparticle type, size and/or SPR
- Loading of all ligands is optimized
- Customer can focus on research and not on Nanopartz™ expertise
- Quick turnaround
"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."
Stephan Link, PhD
Assistant Professor of Chemistry
Noninvasive in vivo spectroscopic nanorod-contrast photoacoustic mapping of sentinel lymph nodes
mapping using gold nanorods as lymph node tracers in a ... As lymph node tracers, gold nanorods
(Nanopartz Inc.) were injected on a left forepaw pad in the amount of 0.1 ml at a 979 ...
in cancer tumor therapy. The chapter also talks about the establishing of a basis of safety for the
IV injection of PEG-coated AuNRs for in vivo photothermal cancer therapy in ...
In vivo real-time monitoring of nanoparticle clearance rate from blood circulation using high speed flow cytometry
obtained from Nanopartz Inc., Loveland, CO), had a ... 802-nm-CD45 particles, which targeted the
leukocytes in the circulation, thus, stayed longer in the circulation due to in vivo labeling. ...
In vivo Photothermal Optical Coherence Tomography of Gold Nanorods in the Mouse Eye
Methods 2.1 Gold nanorod synthesis: Polyethylene glycol (PEG)-coated gold nanorods (GNRs)
with peak absorption of 750 nm were purchased from Nanopartz (Loveland, USA). ...
Modelling and characterization of Photothermal effects assisted with Gold Nanorods in ex-vivo samples and in a murine model
OS-530-LE, Omega) in ex-vivo samples hypodermically injected with nanoparticles
(Ntracker Gold Nanorods, 30-PM-850, OD=50, Nanopartz). ...
Selective inactivation of enzymes conjugated to nanoparticles using tuned laser illumination
detected ex vivo by atomic absorption methods, and in vivo by CT imaging . ... Methods. Materials.
Gold nanospheres were purchased from Nanopartz (cat. No. ...
[PDF] HIGH-SENSITIVE BIOACTIVITY ASSAY FOR HYBRID NANOSTRUCTURED MATERIALS WITH PLASMONIC PROPERTIES
less than 10 ppb (according to the manufacturer); Ntheraphy in-vivo Gold Nanorods,
neutravidin-polymer (Product Nr.: 30-PN-750, Nano- partz), 50 OD-mL (1.6 mg/mL). ...
Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution
such as gold nanoparti- cles, promises in vivo detection of ... the system's lateral resolution by
imaging Au nanowire particles (product number 50-30-6000, 30 by 6000nm, Nanopartz, Inc ...
Enhanced infrared neural stimulation using localized surface plasmon resonance of gold nanorods
potentials of in vivo neural tissues ... Nanoparticle Preparation and Characterization: Rod-shaped
gold nanoparticles (C12–10–980, Nanopartz Inc, Loveland, CO, USA) with a concentration ...
[PDF] SNNI's Proactive Approach to Healthier and Safer of Nanomaterials
- Custom Conjugations www.NanoPartz.com Biotech ... SNNI researchers at Oregon State University
have developed a rapid throughput, in vivo system for delineating the effects of ...