Fluorophore Labeled Gold Nanoparticles

 

 

 

Representative Construct for Fluorescently Labeled Gold Nanoparticles     Conjugations (Ordering Abbreviation) Amine TA Biotin TB Carboxyl TC Maleimide TMAL Methyl TM Neutravidin TN NHS TNHS Streptavidin TS Secondary antibodies

 

Composition

These gold nanoparticles for in vitro dark field, bright field, and fluorescence imaging come in numerous conjugations:

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

1. Amine
2. Biotin
3. Carboxy
4. Maleimide
5. Methyl
6. Neutravidin
7. NHS
8. Streptavidin
9. Azide

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purification methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

1. Goat Anti-Human IgG
2. Goat Anti-Mouse IgG
3. Goat Anti-Rabitt IgG
4. Goat Anti-Human IgA
5. Goat Anti-Human IgM
6. Protein A

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

 

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of conjugations, specifically suited for many applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

Nanopartz™ Fluorescence Labeled Gold Nanoparticles Stability (salt,pH,chemicals) High Scattering linewidth Narrow (monodisperse) Multilabeling Yes (500nm-2100nm) Nonspecific binding Very low pH and salt resistance High Sterilization Radiation Site specific antibody conjugation Yes

• No sodium azide
• No BSA
• Stable
• Well Characterized
• Customer can select buffer
• Customer can select gold nanoparticle type, size and/or SPR
• Loading of all ligands is optimized

• Customer can focus on research
• Quick turnaround

How much thickness does your proprietary capping agent add?

It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Should I store in the refrigerator?

Yes.

What is the total number of fluorophores on the gold nanoparticles?

Because of the many competing factors regarding fluorescence, ie instrument setup, bleaching, scattering, absorption, and quenching, it is impossible and not feasible to provide an exact number. The customer should measure the fluorescence from a known sample of nanorods given by UV VIS and calculate the fluorescence signal/nanorod in their application (ie salt, pH, and other controlling properties) instead, treating it like a composite product.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

"Nanopartz is sublime for their broad range of high quality products, which are, as they truthfully describe on their website of excellent quality. The fluorescent spherical gold nanoparticles I'm using in my research are perfect: high density, stable and high fluorescent efficiency, so they're making my nanoparticle microscopy study so much easier. Moreover, their technical support is the best I have ever encountered in a company, very patient and extremely helpful and friendly. Thank you! "

Wieteke de Boer, PhD Departments of Biological Sciences and Neuroscience Columbia University