Fluorophore Labeled Gold Nanoparticles
This technology utilizes the complete line of Accurate™ Spherical Gold Nanoparticles, Nanorods, Microgold™, and Gold Nanowires and allows the customer to choose the fluorophore and in vitro conjugation including amine, biotin, carboxyl, methyl, maleimide, neutravidin, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. These conjugated particles can be used in brightfield, darkfield, fluorescence, and other imaging modes; and ELISA, lateral flow, and other assays. Although some fluorophores may be plasmonically enhanced by the correct choice of shape and sized gold nanoparticle, the designs allow for as many fluorophores as possible to be conjugated to each, and that there be no quenching of the fluorescence. Every batch is completely characterized including size, monodispersity, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data.
"Nanopartz is sublime for their broad range of high quality products, which are, as they truthfully describe on their website of excellent quality. The fluorescent gold nanoparticles I'm using in my research are perfect: high density, stable and high fluorescent efficiency, so they're making my nanoparticle microscopy study so much easier. Moreover, their technical support is the best I have ever encountered in a company, very patient and extremely helpful and friendly. Thank you! "
Wieteke de Boer, PhD Departments of Biological Sciences and Neuroscience Columbia University
Part # | Diameter (nm) | Peak SPR Wavelength (nm) | Optical Density (1cm) | NPS/ml | Wt. conc (mg/ml) | Wt. % | PPM | Molarity (pM) | Molar Ext. (M-1cm-1) | AEsorption Molar Ext. (M-1cm-1) | Scattering Molar Ext. (M-1cm-1) | Size Dispersity %PDI | Notes |
CF11-1.8 | 1.8 | 505-508 | 50 | 4.25E+16 | 2.5 | 0.25% | 2500 | 7.10E+07 | 7.05E+05 | 7.05E+05 | 0.00E+00 | <35% | 1,3 |
CF11-2.2 | 2.2 | 505-508 | 50 | 2.33E+16 | 2.5 | 0.25% | 2500 | 3.90E+07 | 1.29E+06 | 1.29E+06 | 0.00E+00 | <25% | 1,3 |
CF11-3 | 3 | 505-508 | 50 | 9.19E+15 | 2.5 | 0.25% | 2500 | 1.50E+07 | 3.26E+06 | 3.26E+06 | 0.00E+00 | <20% | 1,3 |
CF11-4 | 4 | 505-508 | 50 | 3.88E+15 | 2.5 | 0.25% | 2500 | 6.50E+06 | 7.74E+06 | 7.74E+06 | 0.00E+00 | <20% | 1,3 |
CF11-5 | 5 | 505-508 | 50 | 1.99E+15 | 2.5 | 0.25% | 2500 | 3.30E+06 | 1.51E+07 | 1.51E+07 | 0.00E+00 | <20% | 2,3 |
CF11-10 | 10 | 514-520 | 50 | 2.48E+14 | 2.5 | 0.25% | 2500 | 4.10E+05 | 1.21E+08 | 1.21E+08 | 0.00E+00 | <15% | 2,3 |
CF11-15 | 15 | 514-520 | 50 | 7.35E+13 | 2.5 | 0.25% | 2500 | 1.20E+05 | 4.08E+08 | 4.08E+08 | 0.00E+00 | <15% | 2,3 |
CF11-20 | 20 | 514-520 | 50 | 3.10E+13 | 2.5 | 0.25% | 2500 | 5.20E+04 | 9.67E+08 | 9.67E+08 | 0.00E+00 | <10% | 2,3 |
CF11-25 | 25 | 514-520 | 50 | 1.59E+13 | 2.5 | 0.25% | 2500 | 2.60E+04 | 1.89E+09 | 1.89E+09 | 0.00E+00 | <10% | 2 |
CF11-30 | 30 | 523 | 50 | 9.19E+12 | 2.5 | 0.25% | 2500 | 1.50E+04 | 3.26E+09 | 3.26E+09 | 0.00E+00 | <6% | 2 |
CF11-35 | 35 | 526 | 50 | 5.79E+12 | 2.5 | 0.25% | 2500 | 9.60E+03 | 5.18E+09 | 5.18E+09 | 0.00E+00 | <6% | 2 |
CF11-40 | 40 | 527 | 50 | 3.88E+12 | 2.5 | 0.25% | 2500 | 6.50E+03 | 7.74E+09 | 7.72E+09 | 2.24E+07 | <4% | 2 |
CF11-45 | 45 | 529 | 50 | 2.72E+12 | 2.5 | 0.25% | 2500 | 4.50E+03 | 1.10E+10 | 1.08E+10 | 2.03E+08 | <4% | 2 |
CF11-50 | 50 | 531 | 50 | 1.99E+12 | 2.5 | 0.25% | 2500 | 3.30E+03 | 1.51E+10 | 1.45E+10 | 5.88E+08 | <4% | 2 |
CF11-55 | 55 | 533 | 50 | 1.49E+12 | 2.5 | 0.25% | 2500 | 2.50E+03 | 2.01E+10 | 1.88E+10 | 1.30E+09 | <4% | 2 |
CF11-60 | 60 | 536 | 50 | 1.15E+12 | 2.5 | 0.25% | 2500 | 1.90E+03 | 2.61E+10 | 2.36E+10 | 2.48E+09 | <4% | 2 |
CF11-65 | 65 | 539 | 50 | 9.04E+11 | 2.5 | 0.25% | 2500 | 1.50E+03 | 3.32E+10 | 2.89E+10 | 4.33E+09 | <4% | 2 |
CF11-70 | 70 | 542 | 50 | 7.23E+11 | 2.5 | 0.25% | 2500 | 1.20E+03 | 4.15E+10 | 3.44E+10 | 7.09E+09 | <4% | 2 |
CF11-75 | 75 | 545 | 50 | 5.88E+11 | 2.5 | 0.25% | 2500 | 9.80E+02 | 5.10E+10 | 4.00E+10 | 1.10E+10 | <4% | 2 |
CF11-80 | 80 | 549 | 50 | 4.85E+11 | 2.5 | 0.25% | 2500 | 8.10E+02 | 6.19E+10 | 4.54E+10 | 1.65E+10 | <4% | 2 |
CF11-85 | 85 | 553 | 50 | 4.04E+11 | 2.5 | 0.25% | 2500 | 6.70E+02 | 7.42E+10 | 5.03E+10 | 2.39E+10 | <4% | 2 |
CF11-90 | 90 | 558 | 50 | 3.40E+11 | 2.5 | 0.25% | 2500 | 5.70E+02 | 8.81E+10 | 5.44E+10 | 3.37E+10 | <4% | 2 |
CF11-95 | 95 | 563 | 50 | 2.89E+11 | 2.5 | 0.25% | 2500 | 4.80E+02 | 1.04E+11 | 5.72E+10 | 4.65E+10 | <4% | 2 |
CF11-100 | 100 | 569 | 50 | 2.48E+11 | 2.5 | 0.25% | 2500 | 4.10E+02 | 1.21E+11 | 5.82E+10 | 6.27E+10 | <4% | 2 |
CF11-125 | 125 | 600 | 50 | 1.27E+11 | 2.5 | 0.25% | 2500 | 2.10E+02 | 2.36E+11 | 1.53E+11 | 8.32E+10 | <4% | 2 |
CF11-150 | 150 | 612 | 50 | 7.35E+10 | 2.5 | 0.25% | 2500 | 1.20E+02 | 4.08E+11 | 2.64E+11 | 1.44E+11 | <4% | 2 |
Notes: | |
1 |
Size accuracy +/- 0.1 nm |
1 |
Capping agent is covalently bonded carboxyl |
2 |
Size accuracy +/- 2 nm |
3 |
Peak SPR does not determine size for d<20 nm |
Vol is 25mL, 100mL, 500mL, 1000mL | |
%PDI = Std Dev/Size | |
See Tech Note TN801 for definition of terms and method of analysis | |
All specs typical. May vary batch to batch. Exact values are measured for each batch | |
OD >=50 for all sizes | |
Shape monodispersity (% spheres) > 99.9% | |
Solution is 18MEG DI water | |
Residual Chemicals < 0.1% |
Part # | Diameter (nm) | Length (nm) | Peak SPR Wave (nm) | Aspect Ratio | OD SPR (AU) | Peak LSPR Wave (nm) | OD LSPR (AU) | Nanorod Vol (nm3) | Nanorods /mL | Wt. conc (mg/ml) | Wt. % | PPM | Molarity (nM) | SPR Molar Ext. (M-1cm-1) | LSPR Molar Ext. (M-1cm-1) | Peak SPR accuracy (nm) | SPR Linewidth 80% (nm) |
CF12-10-2100 | 10 | 175 | 2100 | 17.5 | 5 | 510 | 1 | 1.35E+04 | 6.73E+11 | 0.175 | 0.02% | 175 | 1.12 | 4.46E+09 | 8.92E+08 | 1900-2300 | 300 |
CF12-10-1400 | 10 | 102 | 1400 | 10.2 | 5 | 510 | 1 | 7.77E+03 | 1.17E+12 | 0.175 | 0.02% | 175 | 1.95 | 2.57E+09 | 5.14E+08 | 1300-1500 | 150 |
CF12-10-1200 | 10 | 81 | 1200 | 8.1 | 5 | 510 | 1 | 6.13E+03 | 1.48E+12 | 0.175 | 0.02% | 175 | 2.47 | 2.03E+09 | 4.05E+08 | 1132-1300 | 150 |
CF12-10-1064 | 10 | 67 | 1064 | 6.7 | 50 | 510 | 10 | 5.02E+03 | 1.81E+13 | 1.75 | 0.18% | 1750 | 30.13 | 1.66E+09 | 3.32E+08 | 1022-1132 | 150 |
CF12-10-980 | 10 | 59 | 980 | 5.9 | 50 | 510 | 10 | 4.33E+03 | 2.09E+13 | 1.75 | 0.18% | 1750 | 34.91 | 1.43E+09 | 2.86E+08 | 965-1022 | 150 |
CF12-10-950 | 10 | 55 | 950 | 5.5 | 50 | 510 | 10 | 4.09E+03 | 2.22E+13 | 1.75 | 0.18% | 1750 | 37 | 1.35E+09 | 2.70E+08 | 925-965 | 150 |
CF12-10-900 | 10 | 50 | 900 | 5 | 50 | 510 | 10 | 3.68E+03 | 2.47E+13 | 1.75 | 0.18% | 1750 | 41.11 | 1.22E+09 | 2.43E+08 | 875-925 | 150 |
CF12-10-850 | 10 | 45 | 850 | 4.5 | 50 | 510 | 10 | 3.27E+03 | 2.78E+13 | 1.75 | 0.18% | 1750 | 46.25 | 1.08E+09 | 2.16E+08 | 829-875 | 100 |
CF12-10-808 | 10 | 41 | 808 | 4.1 | 50 | 510 | 10 | 2.93E+03 | 3.10E+13 | 1.75 | 0.18% | 1750 | 51.68 | 9.68E+08 | 1.94E+08 | 794-829 | 75 |
CF12-10-780 | 10 | 38 | 780 | 3.8 | 50 | 510 | 10 | 2.70E+03 | 3.36E+13 | 1.75 | 0.18% | 1750 | 56.06 | 8.92E+08 | 1.78E+08 | 765-794 | 65 |
CF12-10-750 | 10 | 35 | 750 | 3.5 | 50 | 510 | 10 | 2.45E+03 | 3.70E+13 | 1.75 | 0.18% | 1750 | 61.67 | 8.11E+08 | 1.62E+08 | 725-765 | 50 |
CF12-10-700 | 10 | 29 | 700 | 2.9 | 50 | 510 | 10 | 2.04E+03 | 4.44E+13 | 1.75 | 0.18% | 1750 | 74 | 6.76E+08 | 1.35E+08 | 675-725 | 40 |
CF12-10-650 | 10 | 24 | 650 | 2.4 | 50 | 510 | 10 | 1.64E+03 | 5.55E+13 | 1.75 | 0.18% | 1750 | 92.5 | 5.41E+08 | 1.08E+08 | 625-675 | 40 |
CF12-10-600 | 10 | 19 | 600 | 1.9 | 50 | 510 | 10 | 1.23E+03 | 7.40E+13 | 1.75 | 0.18% | 1750 | 123.34 | 4.05E+08 | 8.11E+07 | 575-625 | 40 |
CF12-25-1400 | 25 | 245 | 1400 | 9.8 | 5 | 514 | 1 | 1.16E+05 | 1.12E+11 | 0.25 | 0.03% | 250 | 0.19 | 2.69E+10 | 5.37E+09 | 1232-1500 | 150 |
CF12-25-1064 | 25 | 137 | 1064 | 5.5 | 5 | 514 | 1 | 6.31E+04 | 2.05E+11 | 0.25 | 0.03% | 250 | 0.34 | 1.46E+10 | 2.92E+09 | 1022-1232 | 150 |
CF12-25-980 | 25 | 119 | 980 | 4.8 | 50 | 514 | 10 | 5.43E+04 | 2.39E+12 | 2.5 | 0.25% | 2500 | 3.98 | 1.26E+10 | 2.51E+09 | 965-1022 | 150 |
CF12-25-950 | 25 | 102 | 950 | 4.1 | 50 | 514 | 10 | 4.60E+04 | 2.82E+12 | 2.5 | 0.25% | 2500 | 4.7 | 1.06E+10 | 2.13E+09 | 925-965 | 150 |
CF12-25-900 | 25 | 96 | 900 | 3.8 | 50 | 514 | 10 | 4.30E+04 | 3.01E+12 | 2.5 | 0.25% | 2500 | 5.02 | 9.95E+09 | 1.99E+09 | 875-925 | 150 |
CF12-25-850 | 25 | 93 | 850 | 3.7 | 50 | 514 | 10 | 4.15E+04 | 3.12E+12 | 2.5 | 0.25% | 2500 | 5.2 | 9.61E+09 | 1.92E+09 | 829-875 | 150 |
CF12-25-808 | 25 | 90 | 808 | 3.6 | 50 | 514 | 10 | 4.01E+04 | 3.24E+12 | 2.5 | 0.25% | 2500 | 5.39 | 9.27E+09 | 1.85E+09 | 794-829 | 150 |
CF12-25-780 | 25 | 87 | 780 | 3.5 | 50 | 514 | 10 | 3.86E+04 | 3.36E+12 | 2.5 | 0.25% | 2500 | 5.6 | 8.93E+09 | 1.79E+09 | 765-794 | 150 |
CF12-25-750 | 25 | 85 | 750 | 3.4 | 50 | 514 | 10 | 3.76E+04 | 3.45E+12 | 2.5 | 0.25% | 2500 | 5.75 | 8.70E+09 | 1.74E+09 | 725-765 | 65 |
CF12-25-700 | 25 | 75 | 700 | 3 | 50 | 514 | 10 | 3.27E+04 | 3.96E+12 | 2.5 | 0.25% | 2500 | 6.61 | 7.57E+09 | 1.51E+09 | 675-725 | 60 |
CF12-25-650 | 25 | 71 | 650 | 2.8 | 50 | 514 | 10 | 3.07E+04 | 4.22E+12 | 2.5 | 0.25% | 2500 | 7.03 | 7.11E+09 | 1.42E+09 | 625-675 | 60 |
CF12-25-600 | 25 | 57 | 600 | 2.3 | 50 | 514 | 10 | 2.39E+04 | 5.43E+12 | 2.5 | 0.25% | 2500 | 9.05 | 5.52E+09 | 1.10E+09 | 575-625 | 60 |
CF12-25-550 | 25 | 34 | 550 | 1.4 | 50 | 514 | 10 | 1.28E+04 | 1.01E+13 | 2.5 | 0.25% | 2500 | 16.91 | 2.96E+09 | 5.91E+08 | 525-575 | 60 |
CF12-40-850 | 40 | 148 | 850 | 3.7 | 50 | 520 | 10 | 1.69E+05 | 7.67E+11 | 2.5 | 0.25% | 2500 | 1.28 | 3.91E+10 | 7.83E+09 | 829-875 | 60 |
CF12-40-808 | 40 | 134 | 808 | 3.4 | 50 | 520 | 10 | 1.52E+05 | 8.56E+11 | 2.5 | 0.25% | 2500 | 1.43 | 3.51E+10 | 7.01E+09 | 794-829 | 60 |
CF12-40-780 | 40 | 124 | 780 | 3.1 | 50 | 520 | 10 | 1.39E+05 | 9.33E+11 | 2.5 | 0.25% | 2500 | 1.55 | 3.22E+10 | 6.43E+09 | 765-794 | 60 |
CF12-40-750 | 40 | 112 | 750 | 2.8 | 50 | 520 | 10 | 1.24E+05 | 1.05E+12 | 2.5 | 0.25% | 2500 | 1.74 | 2.87E+10 | 5.73E+09 | 725-765 | 60 |
CF12-40-700 | 40 | 92 | 700 | 2.3 | 50 | 520 | 10 | 9.88E+04 | 1.31E+12 | 2.5 | 0.25% | 2500 | 2.19 | 2.29E+10 | 4.57E+09 | 675-725 | 60 |
CF12-40-650 | 40 | 80 | 650 | 2 | 50 | 520 | 10 | 8.37E+04 | 1.55E+12 | 2.5 | 0.25% | 2500 | 2.58 | 1.94E+10 | 3.87E+09 | 625-675 | 60 |
CF12-40-600 | 40 | 68 | 600 | 1.7 | 50 | 520 | 10 | 6.87E+04 | 1.89E+12 | 2.5 | 0.25% | 2500 | 3.15 | 1.59E+10 | 3.18E+09 | 575-625 | 60 |
CF12-40-550 | 40 | 60 | 550 | 1.5 | 50 | 520 | 10 | 5.86E+04 | 2.21E+12 | 2.5 | 0.25% | 2500 | 3.69 | 1.36E+10 | 2.71E+09 | 525-575 | 60 |
CF12-50-800 | 50 | 145 | 800 | 2.9 | 50 | 530 | 10 | 2.52E+05 | 5.15E+11 | 2.5 | 0.25% | 2500 | 0.86 | 5.83E+10 | 1.17E+10 | 750-825 | 60 |
CF12-50-700 | 50 | 110 | 700 | 2.2 | 50 | 530 | 10 | 1.83E+05 | 7.08E+11 | 2.5 | 0.25% | 2500 | 1.18 | 4.24E+10 | 8.48E+09 | 650-750 | 60 |
CF12-50-600 | 50 | 100 | 600 | 2 | 50 | 530 | 10 | 1.64E+05 | 7.93E+11 | 2.5 | 0.25% | 2500 | 1.32 | 3.78E+10 | 7.57E+09 | 550-650 | 60 |
Part # | Diameter (nm) | Length (nm) | Aspect Ratio | Peak SPR Wave (nm) | SPR OD (1cm) | Microgold Vol (nm3) | Microgold /mL | Wt. conc (ug/ml) | Wt. % | PPM | Molarity (pM) | SPR Molar Ext. (M-1cm-1) |
CF13-500 | 75 | 500 | 6.7 | 510 | 0.25 | 2.10E+06 | 1.24E+09 | 50 | 0.01% | 50 | 2.06 | 1.21E+08 |
CF13-1000 | 100 | 1000 | 10 | 510 | 0.25 | 7.59E+06 | 3.42E+08 | 50 | 0.01% | 50 | 0.57 | 4.39E+08 |
Part # | Diameter (nm) | Length (nm) | Aspect Ratio | Peak SPR Wave (nm) | Nanowires Vol (nm3) | Nanowires /mL | Wt. conc (ug/ml) | Wt. % | PPM | Molarity (pM) | SPR Molar Ext. (M-1cm-1) |
CF14-1000 | 75 | 1000 | 13 | 510 | 4.31E+06 | 3.01E+09 | 250 | 0.03% | 250 | 5.02 | 9.96E+06 |
CF14-2000 | 75 | 2000 | 27 | 510 | 8.72E+06 | 1.49E+09 | 250 | 0.03% | 250 | 2.48 | 2.02E+07 |
CF14-4000 | 75 | 4000 | 53 | 510 | 1.76E+07 | 7.39E+08 | 250 | 0.03% | 250 | 1.23 | 4.06E+07 |
CF14-6000 | 75 | 6000 | 80 | 510 | 2.64E+07 | 4.91E+08 | 250 | 0.03% | 250 | 0.82 | 6.10E+07 |
CF14-10000 | 75 | 10000 | 133 | 510 | 4.40E+07 | 2.94E+08 | 250 | 0.03% | 250 | 0.49 | 1.02E+08 |
Wavelength | Fluorophore Name | Excitation (nm) | Emission (nm) | Ext. Coef | QY |
405 | AF405 | 402 | 421 | ||
Dy405 | 400 | 420 | |||
488 | FITC | 494 | 518 | 70000 | |
Dy488 | 493 | 518 | 70000 | ||
AF488 | 490 | 525 | |||
514 | NPZ514 | 520 | 540 | ||
532 | Rhodamine | 535 | 575 | ||
550 | Cy3 | 555 | 570 | 150000 | 0.31 |
Dy550 | 562 | 576 | |||
AF555 | 555 | 580 | |||
600 | Cy3.5 | 591 | 604 | 116000 | 0.35 |
Dy594 | 593 | 618 | |||
AF594 | 590 | 617 | |||
Texas Red | 592 | 614 | |||
650 | Cy5 | 646 | 662 | 250000 | 0.2 |
Dy 650 | 652 | 672 | |||
AF647 | 650 | 665 | |||
680 | Cy5.5 | 673 | 707 | 209000 | 0.2 |
Dy680 | 682 | 715 | |||
AF680 | 679 | 702 | |||
750 | Cy7 | 750 | 773 | 199000 | 0.3 |
Dy755 | 754 | 776 | 220000 | ||
AF750 | 749 | 775 | |||
800 | Cy7.5 | 788 | 808 | 223000 | |
Dy800 | 777 | 794 | |||
QDOT 800 | 633 | 800 |
Representative Construct for Fluorescently Labeled Gold Nanoparticles
Conjugations (Ordering Abbreviation)
|
Composition
These gold nanoparticles for in vitro dark field, bright field, and fluorescence imaging come in numerous conjugations:
Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.
- Amine
- Biotin
- Carboxy
- Maleimide
- Methyl
- Neutravidin
- NHS
- Streptavidin
- Azide
Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purification methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.
- Goat Anti-Human IgG
- Goat Anti-Mouse IgG
- Goat Anti-Rabitt IgG
- Goat Anti-Human IgA
- Goat Anti-Human IgM
- Protein A
Quantity
This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.
Delivery
All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.
Shelf Life/Storage Temperature
This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.
Sterilization/Endotoxin
Sterilization and Endoxtoin Free Options are available.
Certifications
Every order comes with a Certification of Analysis. We use NIST traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of conjugations, specifically suited for many applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.
|
- No sodium azide
- No BSA
- Stable
- Well Characterized
- Customer can select buffer
- Customer can select gold nanoparticle type, size and/or SPR
- Loading of all ligands is optimized
- Customer can focus on research
- Quick turnaround
Representative Construct for Fluorescently Labeled Gold Nanoparticles
How much thickness does your proprietary capping agent add?
It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.
Why do you sell your rods as OD-mLs and what does this mean?
This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.
Can I resuspend in water?
Yes
Should I store in the refrigerator?
Yes.
What is the total number of fluorophores on the gold nanoparticles?
Because of the many competing factors regarding fluorescence, ie instrument setup, bleaching, scattering, absorption, and quenching, it is impossible and not feasible to provide an exact number. The customer should measure the fluorescence from a known sample of nanorods given by UV VIS and calculate the fluorescence signal/nanorod in their application (ie salt, pH, and other controlling properties) instead, treating it like a composite product.
"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."
Stephan Link, PhD
Assistant Professor of Chemistry
Rice University
"Nanopartz is sublime for their broad range of high quality products, which are, as they truthfully describe on their website of excellent quality. The fluorescent spherical gold nanoparticles I'm using in my research are perfect: high density, stable and high fluorescent efficiency, so they're making my nanoparticle microscopy study so much easier. Moreover, their technical support is the best I have ever encountered in a company, very patient and extremely helpful and friendly. Thank you! "
Wieteke de Boer, PhD Departments of Biological Sciences and Neuroscience Columbia University
Gold nanoparticles enhanced electroporation for mammalian cell transfection
design of AuNPs (from Nanopartz, Inc., having fluorophores separated from the gold surface
with polymer spacer) were used to circumvent this problem. Fluorophore labeled AuNPs ...
Gold nanoparticles electroporation enhanced polyplex delivery to mammalian cells
design of fluorophore-labeled AuNPs (FNP from Nanopartz, having Alexa Fluor 546) with
fluorophores separated from the gold surface by polymer spacers, were used to ...
[PDF] HIGH-SENSITIVE BIOACTIVITY ASSAY FOR HYBRID NANOSTRUCTURED MATERIALS WITH PLASMONIC PROPERTIES
Proteins possess three intrinsic fluorophores: phenylalanine, tyrosine and tryptophan ... Ntheraphy
in-vivo Gold Nanorods, neutravidin-polymer (Product Nr.: 30-PN-750, Nano- partz), 50 OD ...
[PDF] ZEISS ELYRA
[www.nanopartz.com] Nanorods 550 (Cat. No. ... Sample Preparation and Fluorophore Properties
for dSTORM 1. GT Dempsey et al.: Evaluation of fluorophores for optimal performance ...
[HTML] Nanoparticle amplification via photothermal unveiling of cryptic collagen binding sites
functionalized with either FnF or albumin. ... Bare gold nanorods (30-10-808, Nanopartz, Loveland,
CO), 10 nm axial diameter × 41 nm length, were ... Fluorophore-conjugated proteins. ...
Plasmonic nanoprobes for stimulated emission depletion microscopy
featuring approximately 15 ... is the unit dipole moment in the direction of the fluorophore's dipole
and ... of 8.5, which corresponds to an average field enhancement for the fluorophores of 3 ...
Lab-in-fiber platform for plasmonic photothermal study
excitation of plasmonic photothermal effects in the similar release of liposome-encapsulated
fluorophores. ... 53nm gold nanoparticles were obtained from Nanopartz (Loveland, Colorado ...
[HTML] A nanobiosensor for dynamic single cell analysis during microvascular self-organization
detection. ... GNRs (Nanopartz) with 10 nm axial diameter and 67 nm length were modified
with mercaptoundecyltrimethylammonium bromide (MUTAB). ...
Brightness and Photostability of Emerging Red and Near‐IR Fluorescent Nanomaterials for Bioimaging
remains ... is needed?; how bright will it be relative to a common fluorophore?; and what ... Carbon
dots, Graphite, oxygen, nitrogen, 6, 530, 655, Carboxyl, hydroxyl, amine, No, Nanopartz. ...