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Fluorophore Labeled Gold Nanoparticles

This technology utilizes the complete line of Accurate™ Spherical Gold Nanoparticles, Nanorods, Microgold™, and Gold Nanowires and allows the customer to choose the fluorophore and in vitro conjugation including amine, biotin, carboxyl, methyl, maleimide, neutravidin, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. These conjugated particles can be used in brightfield, darkfield, fluorescence, and other imaging modes; and ELISA, lateral flow, and other assays. Although some fluorophores may be plasmonically enhanced by the correct choice of shape and sized gold nanoparticle, the designs allow for as many fluorophores as possible to be conjugated to each, and that there be no quenching of the fluorescence. Every batch is completely characterized including size, monodispersity, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data.

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CF11 Fluorophore Labeled Spherical Gold Nanoparticles
CF11 Fluorophore Labeled Spherical Gold Nanoparticles
Part Number: CF11-(SIZE)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(BUFFER)-(OD-mLs)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL) Example part number for a 30nm diameter sphere, 550nm fluorophore, with a methyl polymer functionalization, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CF11-30-550-TM-DIH-50
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
CF12 Fluorophore Labeled Gold Nanorods
CF12 Fluorophore Labeled Gold Nanorods
Part Number: CF12-(DIAM/SPR)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(Buffer)-(OD-mLs)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL). Example part number for a 25nm diameter rod with an SPR=808nm, 550nm fluorophore, with a methyl polymer functionalization, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CP12-25-808-550-TM-DIH-50
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
CF13 Fluorophore Labeled Microgold
CF13 Fluorophore Labeled Microgold
Part Number: CF13-(LENGTH)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(Buffer)-5-(Sterilization)-(Endoxtoxin)-(Negative Control). Example part number for a 1000nm long Microgold rod, 550 fluorophore, with a methyl polymer conjugation, in water, at 5 OD-mLs (2.5mg/mL, OD=50, 0.1mL) would be: CF13-1000-550-TM-DIH-5
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
CF14 Fluorophore Labeled Gold Nanowires
CF14 Fluorophore Labeled Gold Nanowires
Part Number: CF14-(LENGTH)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(Buffer)-5-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL). Example part number for a 10000nm long gold nanowire, 550 fluorophore, with a methyl polymer conjugation, in water, at 5 OD-mLs (2.5mg/mL, OD=50, 0.1mL) would be: CF14-10000-550-TM-DIH-5
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

 

 

 

 

Part # Diameter (nm) Peak SPR Wavelength (nm) Optical Density (1cm) NPS/ml Wt. conc (mg/ml) Wt. % PPM Molarity (pM) Molar Ext. (M-1cm-1) AEsorption Molar Ext. (M-1cm-1) Scattering Molar Ext. (M-1cm-1) Size Dispersity %PDI Notes
CF11-1.8 1.8 505-508 50 4.25E+16 2.5 0.25% 2500 7.10E+07 7.05E+05 7.05E+05 0.00E+00 <35% 1,3
CF11-2.2 2.2 505-508 50 2.33E+16 2.5 0.25% 2500 3.90E+07 1.29E+06 1.29E+06 0.00E+00 <25% 1,3
CF11-3 3 505-508 50 9.19E+15 2.5 0.25% 2500 1.50E+07 3.26E+06 3.26E+06 0.00E+00 <20% 1,3
CF11-4 4 505-508 50 3.88E+15 2.5 0.25% 2500 6.50E+06 7.74E+06 7.74E+06 0.00E+00 <20% 1,3
CF11-5 5 505-508 50 1.99E+15 2.5 0.25% 2500 3.30E+06 1.51E+07 1.51E+07 0.00E+00 <20% 2,3
CF11-10 10 514-520 50 2.48E+14 2.5 0.25% 2500 4.10E+05 1.21E+08 1.21E+08 0.00E+00 <15% 2,3
CF11-15 15 514-520 50 7.35E+13 2.5 0.25% 2500 1.20E+05 4.08E+08 4.08E+08 0.00E+00 <15% 2,3
CF11-20 20 514-520 50 3.10E+13 2.5 0.25% 2500 5.20E+04 9.67E+08 9.67E+08 0.00E+00 <10% 2,3
CF11-25 25 514-520 50 1.59E+13 2.5 0.25% 2500 2.60E+04 1.89E+09 1.89E+09 0.00E+00 <10% 2
CF11-30 30 523 50 9.19E+12 2.5 0.25% 2500 1.50E+04 3.26E+09 3.26E+09 0.00E+00 <6% 2
CF11-35 35 526 50 5.79E+12 2.5 0.25% 2500 9.60E+03 5.18E+09 5.18E+09 0.00E+00 <6% 2
CF11-40 40 527 50 3.88E+12 2.5 0.25% 2500 6.50E+03 7.74E+09 7.72E+09 2.24E+07 <4% 2
CF11-45 45 529 50 2.72E+12 2.5 0.25% 2500 4.50E+03 1.10E+10 1.08E+10 2.03E+08 <4% 2
CF11-50 50 531 50 1.99E+12 2.5 0.25% 2500 3.30E+03 1.51E+10 1.45E+10 5.88E+08 <4% 2
CF11-55 55 533 50 1.49E+12 2.5 0.25% 2500 2.50E+03 2.01E+10 1.88E+10 1.30E+09 <4% 2
CF11-60 60 536 50 1.15E+12 2.5 0.25% 2500 1.90E+03 2.61E+10 2.36E+10 2.48E+09 <4% 2
CF11-65 65 539 50 9.04E+11 2.5 0.25% 2500 1.50E+03 3.32E+10 2.89E+10 4.33E+09 <4% 2
CF11-70 70 542 50 7.23E+11 2.5 0.25% 2500 1.20E+03 4.15E+10 3.44E+10 7.09E+09 <4% 2
CF11-75 75 545 50 5.88E+11 2.5 0.25% 2500 9.80E+02 5.10E+10 4.00E+10 1.10E+10 <4% 2
CF11-80 80 549 50 4.85E+11 2.5 0.25% 2500 8.10E+02 6.19E+10 4.54E+10 1.65E+10 <4% 2
CF11-85 85 553 50 4.04E+11 2.5 0.25% 2500 6.70E+02 7.42E+10 5.03E+10 2.39E+10 <4% 2
CF11-90 90 558 50 3.40E+11 2.5 0.25% 2500 5.70E+02 8.81E+10 5.44E+10 3.37E+10 <4% 2
CF11-95 95 563 50 2.89E+11 2.5 0.25% 2500 4.80E+02 1.04E+11 5.72E+10 4.65E+10 <4% 2
CF11-100 100 569 50 2.48E+11 2.5 0.25% 2500 4.10E+02 1.21E+11 5.82E+10 6.27E+10 <4% 2
CF11-125 125 600 50 1.27E+11 2.5 0.25% 2500 2.10E+02 2.36E+11 1.53E+11 8.32E+10 <4% 2
CF11-150 150 612 50 7.35E+10 2.5 0.25% 2500 1.20E+02 4.08E+11 2.64E+11 1.44E+11 <4% 2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >=50 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) Peak LSPR Wave (nm) OD LSPR (AU) Nanorod Vol (nm3) Nanorods /mL Wt. conc (mg/ml) Wt. % PPM Molarity (nM) SPR Molar Ext. (M-1cm-1) LSPR Molar Ext. (M-1cm-1) Peak SPR accuracy (nm) SPR Linewidth 80% (nm)
CF12-10-2100 10 175 2100 17.5 5 510 1 1.35E+04 6.73E+11 0.175 0.02% 175 1.12 4.46E+09 8.92E+08 1900-2300 300
CF12-10-1400 10 102 1400 10.2 5 510 1 7.77E+03 1.17E+12 0.175 0.02% 175 1.95 2.57E+09 5.14E+08 1300-1500 150
CF12-10-1200 10 81 1200 8.1 5 510 1 6.13E+03 1.48E+12 0.175 0.02% 175 2.47 2.03E+09 4.05E+08 1132-1300 150
CF12-10-1064 10 67 1064 6.7 50 510 10 5.02E+03 1.81E+13 1.75 0.18% 1750 30.13 1.66E+09 3.32E+08 1022-1132 150
CF12-10-980 10 59 980 5.9 50 510 10 4.33E+03 2.09E+13 1.75 0.18% 1750 34.91 1.43E+09 2.86E+08 965-1022 150
CF12-10-950 10 55 950 5.5 50 510 10 4.09E+03 2.22E+13 1.75 0.18% 1750 37 1.35E+09 2.70E+08 925-965 150
CF12-10-900 10 50 900 5 50 510 10 3.68E+03 2.47E+13 1.75 0.18% 1750 41.11 1.22E+09 2.43E+08 875-925 150
CF12-10-850 10 45 850 4.5 50 510 10 3.27E+03 2.78E+13 1.75 0.18% 1750 46.25 1.08E+09 2.16E+08 829-875 100
CF12-10-808 10 41 808 4.1 50 510 10 2.93E+03 3.10E+13 1.75 0.18% 1750 51.68 9.68E+08 1.94E+08 794-829 75
CF12-10-780 10 38 780 3.8 50 510 10 2.70E+03 3.36E+13 1.75 0.18% 1750 56.06 8.92E+08 1.78E+08 765-794 65
CF12-10-750 10 35 750 3.5 50 510 10 2.45E+03 3.70E+13 1.75 0.18% 1750 61.67 8.11E+08 1.62E+08 725-765 50
CF12-10-700 10 29 700 2.9 50 510 10 2.04E+03 4.44E+13 1.75 0.18% 1750 74 6.76E+08 1.35E+08 675-725 40
CF12-10-650 10 24 650 2.4 50 510 10 1.64E+03 5.55E+13 1.75 0.18% 1750 92.5 5.41E+08 1.08E+08 625-675 40
CF12-10-600 10 19 600 1.9 50 510 10 1.23E+03 7.40E+13 1.75 0.18% 1750 123.34 4.05E+08 8.11E+07 575-625 40
CF12-25-1400 25 245 1400 9.8 5 514 1 1.16E+05 1.12E+11 0.25 0.03% 250 0.19 2.69E+10 5.37E+09 1232-1500 150
CF12-25-1064 25 137 1064 5.5 5 514 1 6.31E+04 2.05E+11 0.25 0.03% 250 0.34 1.46E+10 2.92E+09 1022-1232 150
CF12-25-980 25 119 980 4.8 50 514 10 5.43E+04 2.39E+12 2.5 0.25% 2500 3.98 1.26E+10 2.51E+09 965-1022 150
CF12-25-950 25 102 950 4.1 50 514 10 4.60E+04 2.82E+12 2.5 0.25% 2500 4.7 1.06E+10 2.13E+09 925-965 150
CF12-25-900 25 96 900 3.8 50 514 10 4.30E+04 3.01E+12 2.5 0.25% 2500 5.02 9.95E+09 1.99E+09 875-925 150
CF12-25-850 25 93 850 3.7 50 514 10 4.15E+04 3.12E+12 2.5 0.25% 2500 5.2 9.61E+09 1.92E+09 829-875 150
CF12-25-808 25 90 808 3.6 50 514 10 4.01E+04 3.24E+12 2.5 0.25% 2500 5.39 9.27E+09 1.85E+09 794-829 150
CF12-25-780 25 87 780 3.5 50 514 10 3.86E+04 3.36E+12 2.5 0.25% 2500 5.6 8.93E+09 1.79E+09 765-794 150
CF12-25-750 25 85 750 3.4 50 514 10 3.76E+04 3.45E+12 2.5 0.25% 2500 5.75 8.70E+09 1.74E+09 725-765 65
CF12-25-700 25 75 700 3 50 514 10 3.27E+04 3.96E+12 2.5 0.25% 2500 6.61 7.57E+09 1.51E+09 675-725 60
CF12-25-650 25 71 650 2.8 50 514 10 3.07E+04 4.22E+12 2.5 0.25% 2500 7.03 7.11E+09 1.42E+09 625-675 60
CF12-25-600 25 57 600 2.3 50 514 10 2.39E+04 5.43E+12 2.5 0.25% 2500 9.05 5.52E+09 1.10E+09 575-625 60
CF12-25-550 25 34 550 1.4 50 514 10 1.28E+04 1.01E+13 2.5 0.25% 2500 16.91 2.96E+09 5.91E+08 525-575 60
CF12-40-850 40 148 850 3.7 50 520 10 1.69E+05 7.67E+11 2.5 0.25% 2500 1.28 3.91E+10 7.83E+09 829-875 60
CF12-40-808 40 134 808 3.4 50 520 10 1.52E+05 8.56E+11 2.5 0.25% 2500 1.43 3.51E+10 7.01E+09 794-829 60
CF12-40-780 40 124 780 3.1 50 520 10 1.39E+05 9.33E+11 2.5 0.25% 2500 1.55 3.22E+10 6.43E+09 765-794 60
CF12-40-750 40 112 750 2.8 50 520 10 1.24E+05 1.05E+12 2.5 0.25% 2500 1.74 2.87E+10 5.73E+09 725-765 60
CF12-40-700 40 92 700 2.3 50 520 10 9.88E+04 1.31E+12 2.5 0.25% 2500 2.19 2.29E+10 4.57E+09 675-725 60
CF12-40-650 40 80 650 2 50 520 10 8.37E+04 1.55E+12 2.5 0.25% 2500 2.58 1.94E+10 3.87E+09 625-675 60
CF12-40-600 40 68 600 1.7 50 520 10 6.87E+04 1.89E+12 2.5 0.25% 2500 3.15 1.59E+10 3.18E+09 575-625 60
CF12-40-550 40 60 550 1.5 50 520 10 5.86E+04 2.21E+12 2.5 0.25% 2500 3.69 1.36E+10 2.71E+09 525-575 60
CF12-50-800 50 145 800 2.9 50 530 10 2.52E+05 5.15E+11 2.5 0.25% 2500 0.86 5.83E+10 1.17E+10 750-825 60
CF12-50-700 50 110 700 2.2 50 530 10 1.83E+05 7.08E+11 2.5 0.25% 2500 1.18 4.24E+10 8.48E+09 650-750 60
CF12-50-600 50 100 600 2 50 530 10 1.64E+05 7.93E+11 2.5 0.25% 2500 1.32 3.78E+10 7.57E+09 550-650 60

 

 

 

Part # Diameter (nm) Length (nm) Aspect Ratio Peak SPR Wave (nm) SPR OD (1cm) Microgold Vol (nm3) Microgold /mL Wt. conc (ug/ml) Wt. % PPM Molarity (pM) SPR Molar Ext. (M-1cm-1)
CF13-500 75 500 6.7 510 0.25 2.10E+06 1.24E+09 50 0.01% 50 2.06 1.21E+08
CF13-1000 100 1000 10 510 0.25 7.59E+06 3.42E+08 50 0.01% 50 0.57 4.39E+08

 

 

 

 

Part # Diameter (nm) Length (nm) Aspect Ratio Peak SPR Wave (nm) Nanowires Vol (nm3) Nanowires /mL Wt. conc (ug/ml) Wt. % PPM Molarity (pM) SPR Molar Ext. (M-1cm-1)
CF14-1000 75 1000 13 510 4.31E+06 3.01E+09 250 0.03% 250 5.02 9.96E+06
CF14-2000 75 2000 27 510 8.72E+06 1.49E+09 250 0.03% 250 2.48 2.02E+07
CF14-4000 75 4000 53 510 1.76E+07 7.39E+08 250 0.03% 250 1.23 4.06E+07
CF14-6000 75 6000 80 510 2.64E+07 4.91E+08 250 0.03% 250 0.82 6.10E+07
CF14-10000 75 10000 133 510 4.40E+07 2.94E+08 250 0.03% 250 0.49 1.02E+08

 

 

 

 

 

Fluorophore Type Excitation (nm) Emission (nm) Spectrally Similar Dyes
800 788 808 IRDye 800
750 750 773 Alexa Fluor 750
680 673 707 Alexa Fluor 680, Cy5.5
650 646 662 Alexa Fluor 647, Cy5
633 638 658 Alexa Fluor 633
600 591 604 Alexa Fluor 594, Texas Red
550 555 570 Alexa Fluor 546, Alexa Fluor 555
532 533 556  
514 520 540  
488 494 518 FITC, Alexa Fluor 488

 

 

 

 

Functionalized Nanorod Block Invitro

mutab

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer (top) and Nanopartz™ covalent MUTAB (bottom). Thickness of polymer is less than the size variablity of the gold nanoparticle.

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

Composition

These gold nanoparticles for in vitro dark field, bright field, and fluorescence imaging come in numerous conjugations:

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

  1. Amine
  2. Biotin
  3. Carboxy
  4. Maleimide
  5. Methyl
  6. Neutravidin
  7. NHS
  8. Streptavidin
  9. Azide
  10. MUTAB

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purification methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM
  6. Protein A

Custom Conjugations are also available including HRP and GSH.

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

 

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

 

 

  Nanopartz™ Fluroescence Labeled Gold Nanoparticles Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized
  • Customer can focus on research and not on Nanopartz expertise
  • Quick turnaround

microgold in vitro

Representative Construct for Fluorescently Labeled Gold Nanoparticles

SDS

How much thickness does your proprietary capping agent add?

It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

No

Will the SPR wavelength shift with different solvents?

The SPR is a function of the index of refraction of the host solvent in which the nanoparticles are suspended. Nanorods are the most sensitive and are therefore a great tool for measuring the index of refraction of the host.

What is the shelf life if I never open the package?

1 year.

Should I store in the refrigerator?

Yes.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

Gold nanoparticles enhanced electroporation for mammalian cell transfection

Y Zu, S Huang, WC Liao, Y Lu… - Journal of biomedical …, 2014 - ingentaconnect.com
... AuNPs are well known to quench the fluores- cent signal from proximal fluroprobes, a sandwich
design of AuNPs (from Nanopartz, Inc., having fluorophores separated from the gold surface
with polymer spacer) were used to circumvent this problem. Fluorophore labeled AuNPs ...

 

Gold nanoparticles electroporation enhanced polyplex delivery to mammalian cells

S Huang, H Deshmukh, KK Rajagopalan… - …, 2014 - Wiley Online Library
... As AuNPs are well known to quench the fluorescent signal from proximal fluroprobes, a sandwich
design of fluorophore-labeled AuNPs (FNP from Nanopartz, having Alexa Fluor 546) with
fluorophores separated from the gold surface by polymer spacers, were used to ...

 

[PDF] HIGH-SENSITIVE BIOACTIVITY ASSAY FOR HYBRID NANOSTRUCTURED MATERIALS WITH PLASMONIC PROPERTIES

JL Hernandez-Lopez - Latin American Applied Research, 2014 - researchgate.net
... Г + Г = nr m m k τ (4) These equations result in unusual predictions for a fluorophore near a ...
Proteins possess three intrinsic fluorophores: phenylalanine, tyrosine and tryptophan ... Ntheraphy
in-vivo Gold Nanorods, neutravidin-polymer (Product Nr.: 30-PN-750, Nanopartz), 50 OD ...

 

[PDF] ZEISS ELYRA

Q Guide - 2013 - projects.iq.harvard.edu
... No. 790122-010) and 80 nm (Cat. No. 790120-010) nanospheres Au particles • Nanopartz
[www.nanopartz.com] Nanorods 550 (Cat. No. ... Sample Preparation and Fluorophore Properties
for dSTORM 1. GT Dempsey et al.: Evaluation of fluorophores for optimal performance ...

 

[HTML] Nanoparticle amplification via photothermal unveiling of cryptic collagen binding sites

JH Lo, G von Maltzahn, J Douglass, JH Park… - Journal of Materials …, 2013 - pubs.rsc.org
... NWs with amine-conjugated near-infrared tracking fluorophores (VivoTag-S750) were
functionalized with either FnF or albumin. ... Bare gold nanorods (30-10-808, Nanopartz, Loveland,
CO), 10 nm axial diameter × 41 nm length, were ... Fluorophore-conjugated proteins. ...

 

Plasmonic nanoprobes for stimulated emission depletion microscopy

E Cortes, PA Huidobro, HG Sinclair… - arXiv preprint arXiv: …, 2016 - arxiv.org
... Synthesis and Functionalization of AuNRs: Streptavidin coated AuNRs (Nanopartz, 150 μL),
featuring approximately 15 ... is the unit dipole moment in the direction of the fluorophore's dipole
and ... of 8.5, which corresponds to an average field enhancement for the fluorophores of 3 ...

 

Lab-in-fiber platform for plasmonic photothermal study

D Yong, E Lee, WL Ng, X Yu… - SPIE MOEMS- …, 2013 - proceedings.spiedigitallibrary.org
... as a sensor for the optical detection of fluorescence emissions upon fluorophore release from ...
excitation of plasmonic photothermal effects in the similar release of liposome-encapsulated
fluorophores... 53nm gold nanoparticles were obtained from Nanopartz (Loveland, Colorado ...

 

[HTML] A nanobiosensor for dynamic single cell analysis during microvascular self-organization

S Wang, J Sun, DD Zhang, PK Wong - Nanoscale, 2016 - pubs.rsc.org
... The LNA probe was labeled with a fluorophore (6-FAM) at the 5′ end for fluorescence
detection. ... GNRs (Nanopartz) with 10 nm axial diameter and 67 nm length were modified
with mercaptoundecyltrimethylammonium bromide (MUTAB). ...

 

Brightness and Photostability of Emerging Red and Near‐IR Fluorescent Nanomaterials for Bioimaging

P Reineck, A Francis, A Orth, DWM Lau… - Advanced Optical …, 2016 - Wiley Online Library
... The synthesis of efficient, water-soluble, and photostable fluorophores in the near IR (>700 nm)
remains ... is needed?; how bright will it be relative to a common fluorophore?; and what ... Carbon
dots, Graphite, oxygen, nitrogen, 6, 530, 655, Carboxyl, hydroxyl, amine, No, Nanopartz...