image_0
Canine photothermal images of soft tissue sarcomas. Using 10W 808nm CW laser, 100C increases in temperature in 1 second are routinely observed.
  • Canine photothermal images of soft tissue sarcomas. Using 10W 808nm CW laser, 100C increases in temperature in 1 second are routinely observed.
  • Clinical Trial success in Companion Animals in Soft Tissue Sarcomas
  • UV VIS monitoring of canine blood in vivo before and after injection.
  • Measured biodistribution in mice.
  • Mouse photothermal imaging
  • Mouse Therapy
  • Ntracker half life in mice
  • Mouse tumor volume decline after therapy
  • Happy Customer!

Nanopartz™ Ntracker™ for in vivo Therapeutics

Nanopartz™ Ntracker™ for in vivo therapeutics are gold nanorods specifically for use in in vivo applications such as cancer therapy research. These nanorods are coated in a proprietary dense layer of hydrophilic polymers that shield the gold surface and give the particles ultra-long circulation times. The combination of the highly monodisperse gold nanorods with the dense polymer coating extend circulation times 50% longer than other commercial polymers. As opposed to other commercially available nanoparticles such as quantum dots, Ntracker™ nanorods are completely non-toxic. Taking advantage of Enhanced Permeability and Retention effect (EPR), passive tumor targeting is possible where preclinical studies have shown ~7%ID/g tumor accumulation in mice 72 hours post injection, and better in companion animals. This product comes in a diameter of 10nm with SPRs matching all of the popular Near IR CW laser wavelengths, 780, 808, 850, 980, and 1064nm. Every batch is radiation sterilized in PBS, and comes with instructions. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Every product is in stock (99%) and is shipped same day. This product comes in two concentrations, regular OD=50, and highly concentrated OD=250.

Home » D- In Vivo Gold Nanoparticles » DP- In Vivo Photothermal Therapy
 Checkout
Ntherapy
Ntherapy
Part# D12-(SPR)-(Conjugation)-(Wt. conc)
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Price: $480.00
Ntracker
Ntracker
Part# D12M-(SPR)-(Wt.conc)
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Price: $480.00
In-Vivo Plasmonic Fluorophores
In-Vivo Plasmonic Fluorophores
Part # D16-(DIAM-SPR)-(CONJUGATION)-(OD-mLs)
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

 

 

 

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) Peak LSPR Wave (nm) OD LSPR (AU) Nanorod Vol (nm3) Nanorods /mL Wt. conc (mg/ml) Wt. % PPM Molarity (nM) SPR Molar Ext. (M-1cm-1) LSPR Molar Ext. (M-1cm-1) Peak SPR accuracy (nm) SPR Linewidth 80% (nm)
D12M-10-1064 10 67 1064 6.7 50 510 10 5.02E+03 1.81E+13 1.75 0.18% 1750 30.13 1.66E+09 3.32E+08 1022-1132 150
D12M-10-980 10 59 980 5.9 50 510 10 4.33E+03 2.09E+13 1.75 0.18% 1750 34.91 1.43E+09 2.86E+08 965-1022 150
D12M-10-950 10 55 950 5.5 50 510 10 4.09E+03 2.22E+13 1.75 0.18% 1750 37 1.35E+09 2.70E+08 925-965 150
D12M-10-900 10 50 900 5 50 510 10 3.68E+03 2.47E+13 1.75 0.18% 1750 41.11 1.22E+09 2.43E+08 875-925 150
D12M-10-850 10 45 850 4.5 50 510 10 3.27E+03 2.78E+13 1.75 0.18% 1750 46.25 1.08E+09 2.16E+08 829-875 100
D12M-10-808 10 41 808 4.1 50 510 10 2.93E+03 3.10E+13 1.75 0.18% 1750 51.68 9.68E+08 1.94E+08 794-829 75
D12M-10-780 10 38 780 3.8 50 510 10 2.70E+03 3.36E+13 1.75 0.18% 1750 56.06 8.92E+08 1.78E+08 765-794 65

 

  Ntracker Ntherapy
In batch size variation (10nm) < 10% CV < 10% CV
Shape monodispersity >95% nanorods >95% nanorods
Surface charge (zeta) 0 mV Amine +25mV, Carboxyl -5mV typ
pH 7.4 7.4
Residual Chemicals Trace Trace
Toxicity None None
Buffer PBS PBS

 

SPR = Longitudinal SPR peak
LSPR = Lower SPR peak
Shape monodispersity (% rods) > 95%
Aspect ratio variation = Peak SPR accuracy/96
All specs typical
This product is delivered in PBS and is radiation sterilized
Exact loading values are calculated
Exact values are measured for each batch

 

 

 

 

 

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) Peak LSPR Wave (nm) OD LSPR (AU) Nanorod Vol (nm3) Nanorods /mL Wt. conc (mg/ml) Wt. % PPM Molarity (nM) SPR Molar Ext. (M-1cm-1) LSPR Molar Ext. (M-1cm-1) Peak SPR accuracy (nm) SPR Linewidth 80% (nm)
D12-10-1064 10 67 1064 6.7 50 510 10 5.02E+03 1.81E+13 1.75 0.18% 1750 30.13 1.66E+09 3.32E+08 1022-1132 150
D12-10-980 10 59 980 5.9 50 510 10 4.33E+03 2.09E+13 1.75 0.18% 1750 34.91 1.43E+09 2.86E+08 965-1022 150
D12-10-950 10 55 950 5.5 50 510 10 4.09E+03 2.22E+13 1.75 0.18% 1750 37 1.35E+09 2.70E+08 925-965 150
D12-10-900 10 50 900 5 50 510 10 3.68E+03 2.47E+13 1.75 0.18% 1750 41.11 1.22E+09 2.43E+08 875-925 150
D12-10-850 10 45 850 4.5 50 510 10 3.27E+03 2.78E+13 1.75 0.18% 1750 46.25 1.08E+09 2.16E+08 829-875 100
D12-10-808 10 41 808 4.1 50 510 10 2.93E+03 3.10E+13 1.75 0.18% 1750 51.68 9.68E+08 1.94E+08 794-829 75
D12-10-780 10 38 780 3.8 50 510 10 2.70E+03 3.36E+13 1.75 0.18% 1750 56.06 8.92E+08 1.78E+08 765-794 65

 

  Ntracker Ntherapy
In batch size variation (10nm) < 10% CV < 10% CV
Shape monodispersity >95% nanorods >95% nanorods
Surface charge (zeta) 0 mV Amine +25mV, Carboxyl -5mV typ
pH 7.4 7.4
Residual Chemicals Trace Trace
Toxicity None None
Buffer PBS PBS

 

SPR = Longitudinal SPR peak
LSPR = Lower SPR peak
Shape monodispersity (% rods) > 95%
Aspect ratio variation = Peak SPR accuracy/96
All specs typical
This product is delivered in PBS and is radiation sterilized
Exact loading values are calculated
Exact values are measured for each batch

 

 

 

 

 

Part #  Diam (nm) Length (nm) Peak SPR Wave (nm) Ex/Em (nm) Wt. conc (mg/ml) (10-3) Wt. % PPM Molarity (pM) Spectrally Similar Dyes
D16-10-1064 10 67 1064 777/794 1750.0 0.1750% 1750 32.9 IRDye 800
D16-10-980 10 59 980 777/794 1750.0 0.1750% 1750 38.6 IRDye 800
D16-10-850 10 45 850 777/794 1750.0 0.1750% 1750 53.0 IRDye 800
D16-10-808 10 41 808 777/794 1750.0 0.1750% 1750 60.3 IRDye 800
D16-10-780 10 38 780 754/776 1750.0 0.1750% 1750 66.4 Alexa Fluor 750

 

 

 

 

 

Functionalized Nanorod Block Invitro

 

Functionalized gold nanoparticle with Nanopartz™ Ntherapy covalently bonded polymer. Thickness of polymer is less than the size variablity of the gold nanoparticle.

 

Conjugations (Ordering Abbreviation)

 

A33scFv
Chitosan
anti-EGFR
DNA
oligos
Glutathione
Folate
CD33
CD24
CD45
EPCAM
Doxorubicin
T-Cells
anti-CD4
Polyethylenimine (PEI)
Polystyrene

 

A33scFv single-chain antibody selective for colorectal carcinoma cells and used as probes Kirui, D. K., Krishnan, S., Strickland, A. D. and Batt, C. A. , PAA-Derived Gold Nanorods for Cellular Targeting and Photothermal Therapy. Macromolecular Bioscience, n/a. doi: 10.1002/mabi.201100050
Chitosan Multiple thiol/oleyl groups on the polymer backbone introduced multiple anchoring points to the nanoparticle surface and improved the colloidal stability.The primary amine groups on the polymer backbone gave rise to further functionalization possibilities. Erathodiyil Nandanan, Nikhil R. Jana, Jackie Y. Ying  Advanced Materials 2008, 20, 2068–2073
anti-EGFR NRs are functionalized with anti-EGFR antibodies for specific binding to EGFR-positive human oral cancer cells. After exposure to a beam of focused NIR light, the cancer cells were destroyed without affecting the normal cells  Plasmonic photo-thermal therapy (PPTT) Xiaohua Huanga, Mostafa A. El-Sayed Alexandria Journal of Medicine Volume 47, Issue 1, March 2011, Pages 1–9
DNA  NIR irradiation of nanorod–EGFP DNA conjugates had been performed in HeLa cells, the expression of
EGFP in cells was detected at the irradiated spot after NIR exposure at 79 μJ/pulse for 1 min
H. Takahashi, Y. Niidome, S. Yamada, Controlled release of plasmid DNA from gold nanorods induced by pulsed near-infrared light, Chem. Commun. (Camb.) 17 (2005) 2247–2249
oligos selectively release multiple DNA oligonucleotides.with two different DNA oligonucleotides to short and long gold nanorods aspect ratio of t 4.0 and 5.4, corresponding to longitudinal plasmon resonances with light at 800 and 1100 nm respectively irradiated with a laser at the wavelength of 800 nm, only the short gold nanorods were melted but not the long ones. Alternatively, when a laser at the wavelength of 1100 nm was used to irradiate the mixture, the long rods transformed to spherical shapes but not the short ones. A. Wijaya, S.B. Schaffer, I.G. Pallares, K. Hamad-Schifferli, Selective release of
multiple DNA oligonucleotides from gold nanorods, ACS Nano 3 (1) (2008) 80–86.
Glutathione
Folate Conjugates of folic acid with gold nanoparticle could have an important role for folate receptor-targeted
drug delivery or targeted therapy in the future
R. Bhattacharya, C.R. Patra, A. Earl, S. Wang, A. Katarya, L. Lu, J.N. Kizhakkedathu,
M.J. Yaszemski, P.R. Greipp, D. Mukhopadhyay, P. Mukherjee, Attaching folic acid
on gold nanoparticles using noncovalent interaction via different polyethylene
glycol backbones and targeting of cancer cells, Nanomedicine 3 (3) (2007)
224–238.
CD33
CD24
CD45
EPCAM
Doxorubicin
T-Cells Improves cellular uptake invivo [PDF] T cells enhance gold nanoparticle delivery to tumors in vivo LC Kennedy, AS Bear, JK Young, NA Lewinski… - Nanoscale Research …, 2011
anti-CD4
Polyethylenimine (PEI)
Polystyrene

 

 

 

 

 

 

Fluorophore Type Excitation (nm) Emission (nm) Spectrally Similar Dyes
800 788 808 IRDye 800
750 750 773 Alexa Fluor 750

 

 

 

Composition

These polymer coated gold nanorods are shipped in PBS with no measurable residual chemicals. This product is radiation sterilized and is ready for injection.

Custom Formulation

Popular CW laser wavelengths are available (780, 808, 850, 980, and 1064nm).  Other sizes are special order.  Please contact us.

Quantity

This product is available in 1mL and larger but in two different concentrations. The lower concentration is suited for mice, while the higher concentration is suitable for companion animals. For mice, the 1mL injection is suited for 5 injections. For orders larger than 100mL, or for orders amounting over 500mL per annum, please contact sales for quantity pricing.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Conjugation

This product comes coated with a proprietary polymer to increase circulation times. Ntracker™ contains a non-reactive methyl termination. Ntherapy™ contains many choices for reactive terminations. Many popular in vivo conjugations are available.

Introductory Kits

Unfortunately there are no kits available for this product at this time.

Shelf Life/Storage Temperature

This product is guaranteed for one year and may be stored at room temperature.

Toxicity

These products are non cytotoxic.

Sterilization

This product is sterilized.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis that includes the following information. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Ntracker™ is based on extensive research in laboratory, preclinical, toxicology, and more recently clinical trials. The technology is based on patented, patent pending, and proprietary methods. Nanopartz™ has the capacity for up to 1kg of Ntracker™product per day in its audited ISO 9000 facility. Ntherapy™ offers the same technology as Ntracker™ with the added advantage of popular in vivo conjugations and customer specified oligo and antibody choices.

This product has been extensively tested in mice and companion animals.

Photoacoustic Images are provided by our partner Visualsonics Inc.

VisualSonics is the world leader in real-time, in vivo, high-resolution, micro-imaging systems, providing modalities specifically designed for preclinical research. VisualSonics has commercialized Vevo LAZR Technology, a state-of-the-art photoacoustic imaging system with inherent coregistration and capabilities for imaging gold nanorods in vivo. "Listen to the Light" - Photoacoustic imaging with the new Vevo LAZR System.

http://www.visualsonics.com/photoacoustics

Shallow-tissue modalities

Optical coherence tomography (OCT) [http://www.ncbi.nlm.nih.gov/pubmed/7585229]

OCT captures three-dimensional images from optical scattering media (e.g., biological tissue), and it sometimes can provide sub-micrometer resolution. However, the imaging depth in OCT is limited by optical scattering rather than absorption because scattering tends to attenuate and randomize the light. Depending on the wavelength of light, this technique can achieve imaging depths of up to 2 mm in most tissues. This technique has been used clinically for some applications such as eye examination and has been tested in vivo and ex vivo for cancer diagnosis. In order to generate sufficient contrast, the imaging agents for this modality need to have large scattering cross sections.

Photoacoustic tomography (PAT) [http://www.ncbi.nlm.nih.gov/pubmed/20049803]

PAT is a hybrid imaging modality that provides strong optical absorption contrast and high ultrasonic resolution. Because the spatial resolution beyond one optical transport mean free path (~1 mm) is determined by ultrasonic parameters, the maximum imaging depth and resolution of PAT are scalable when diffusive photons are available. One can greatly increase the penetration depth of PAT with near-infrared light because the optical absorption of hemoglobin and scattering of tissues are weak in this regime. Therefore, a proper combination of PAT with the right contrast agent can accurately detect and diagnose tumors. As in the case of OCT, the imaging depth depends on the wavelength of light, but the imaging depth (~30 mm) is higher than OCT. This modality is currently being evaluated in vivo. Additionally, contrast agents for PAT need to have large absorption cross sections.

Two-photon microscopy [http://www.ncbi.nlm.nih.gov/pubmed/2321027]

Two-photon microscopy is a fluorescence-based technique that offers images of living tissue up to ~1 mm in depth. It usually uses red-shifted light to minimize scattering in the tissue, and the background signal is strongly suppressed owing to multiphoton absorption. It is being tested in vivo. Recently, Nguyen and colleagues reported that surgery with molecular fluorescence imaging can be efficient for complete removal of tumors [http://www.ncbi.nlm.nih.gov/pubmed/20160097]. The results might suggest a breakthrough in the application of two-photon microscopy for molecular imaging to overcome the disadvantage of imaging depth.

Surface-enhanced Raman spectroscopy (SERS) imaging [http://www.ncbi.nlm.nih.gov/pubmed/9027306]

SERS utilizes the enhancement of Raman scattering by molecules adsorbed on surfaces of metal NPs. The increment can be as much as 10^14–10^15, hence Raman-active dyes placed on the surface of gold and silver colloids will exhibit greatly amplified Raman signals. The ability of gold colloids to easily conjugate with targeting ligands enables the detection of tumors in vivo using this technique [http://www.ncbi.nlm.nih.gov/pubmed/18157119, http://www.ncbi.nlm.nih.gov/pubmed/19666578]. 

 

 

 

  Nanopartz™ Ntracker™ Other Technologies
Circulation Time Long Low
Toxicity None Broad
Photothermal efficiency Highest recorded Low
Imaging potential Yes Very Limited
Drug Delivery potential Yes Very Limited
Tumor Loading High Low

 

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized
  • Customer can focus on research and not on Nanopartz expertise
  • Quick turnaround

 

 

 

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

Pandia®

C Schoen, C London - … for Biomedical Imaging and Diagnostics: From … - Wiley Online Library
... have been harnessed to develop ultrasensitive diagnostic, spectroscopic, and more recently,
therapeutic technologies. ... There have been a number of studies conducted using Nanopartz AuNRs
that have ... for the IV injection of PEG-coated AuNRs for in vivo photothermal cancer ...
 

Selective fat removal using photothermal heating

A Almutairi, K Almutairi - US Patent App. 15/372,320, 2016 - Google Patents
... to tailor both scattering and absorption of GNRs with different longitudinal surface plasmon
wavelengths is important for therapeutic applications. ... Gold nanorods (GNRs) were procured from
Nanopartz™, specifically “Ntracker™ for in vivo Therapeutics” gold nanorods ...
 

In vivo real-time monitoring of nanoparticle clearance rate from blood circulation using high speed flow cytometry

M Sarimollaoglua, DA Nedosekina… - … of SPIE Vol, 2012 - proceedings.spiedigitallibrary.org
... Two types of conjugated gold nanorods; 695-nm with EpCam and 802-nm with CD45 (both
obtained from Nanopartz Inc., Loveland, CO), had a similar ... Intrinsic therapeutic applications of
noble metal nanoparticles: past, present and future. ... In vivo biodistribution of nanoparticles. ...
 

Modelling and characterization of Photothermal effects assisted with Gold Nanorods in ex-vivo samples and in a murine model

HL Riveraa, FR Jaraa… - Proc. of SPIE …, 2011 - proceedings.spiedigitallibrary.org
... hypodermically injected with nanoparticles (Ntracker Gold Nanorods, 30-PM-850, OD=50,
Nanopartz). ... experimental results of photothermal effects in ex-vivo and in-vivo models. ... and Scott
A. Waldman, Applications of nanoparticles to diagnostics and therapeutics in colorectal ...
 

[CITATION] PASTGIN for diagnosing, monitoring, and treating breast cancer

Y Cheng, A Goktug, K Liu, W Lynk
 

Combined OCT and fluorescence imaging for cancer detection and therapeutic monitoring

Y Chen, J Wierwille, C Roney, B Xu… - … and Laser Science …, 2011 - osapublishing.org
Page 1. Combined OCT and Fluorescence Imaging for Cancer Detection and Therapeutic
Monitoring ... Fig. 2. Co-registered OCT/FMI imaging of intestinal polyps incubated with UEA-1
conjugated liposomes ex vivo... (B) Gold nanospheres (from Nanopartz, Inc., 50 nm in dia.) used ...
 

Gold nanorod photothermal therapy in a genetically engineered mouse model of soft tissue sarcoma

KY LIN, AF BAGLEY, AY ZHANG, DL KARL… - Nano Life, 2010 - World Scientific
... However, characterization of plasmonic nanomaterials as cancer therapeutics has been limited
to xenograft ... demonstrated potential as multimodal diag- nostic and therapeutic agents in
vivo.7,9 ... coated gold NRs with longitudinal plasmon resonance at 810 (Nanopartz Inc.) were ...
 

[HTML] Plasmonic photothermal heating of intraperitoneal tumors through the use of an implanted near-infrared source

AF Bagley, S Hill, GS Rogers, SN Bhatia - ACS nano, 2013 - ncbi.nlm.nih.gov
... Briefly, 41 nm × 10 nm cetyltrimethylammonium (CTAB)-coated GNRs (Nanopartz) were
concentrated ... also acknowledge S. Malstrom from the Koch Institute Applied Therapeutics and
Whole ... Modeling parameters and schematics for NIR light simulations, ex vivo thermography of ...
 

Selective inactivation of enzymes conjugated to nanoparticles using tuned laser illumination

A Ilovitsh, P Polak, Z Zalevsky, O Shefi - Cytometry Part A, 2017 - Wiley Online Library
... to regulate enzyme activity within a mixture as a fundamental building block for biochemical
reactions and therapeutics... nanoparticles can be quantitatively detected ex vivo by atomic
absorption methods, and in vivo by CT ... Gold nanospheres were purchased from Nanopartz (cat ...
 

[HTML] Gold-nanorod contrast-enhanced photoacoustic micro-imaging of focused-ultrasound induced blood-brain-barrier opening in a rat model

PH Wang, HL Liu, PH Hsu, CY Lin… - … of biomedical optics, 2012 - spiedigitallibrary.org