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Canine photothermal images of soft tissue sarcomas. Using 10W 808nm CW laser, 100C increases in temperature in 1 second are routinely observed.
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UV VIS monitoring of canine blood in vivo before and after injection.
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Measured biodistribution in mice.
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Mouse photothermal imaging
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Mouse Therapy
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Ntracker half life in mice
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Mouse tumor volume decline after therapy
Nanopartz™ Ntracker™ for in vivo Therapeutics
Nanopartz™ Ntracker™ for in vivo Mouse therapeutics are gold nanorods specifically for use in in vivo Mouse applications such as cancer therapy research. These nanorods are coated in a proprietary dense layer of hydrophilic polymers that shield the gold surface and give the particles ultra-long circulation times. The combination of the highly monodisperse gold nanorods with the dense polymer coating extend circulation times 50% longer than other commercial polymers. As opposed to other commercially available nanoparticles such as quantum dots, Ntracker™ nanorods are completely non-toxic. Taking advantage of Enhanced Permeability and Retention effect (EPR), passive tumor targeting is possible where preclinical studies have shown ~7%ID/g tumor accumulation in mice 72 hours post injection. This product comes in a diameter of 10nm with SPRs matching all of the popular Near IR CW laser wavelengths, 780, 808, 850, 980, and 1064nm. Every batch is radiation sterilized in PBS, and comes with instructions. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Every product is in stock (99%) and is shipped same day. This product comes in two concentrations, regular OD=50, and highly concentrated OD=250.
NOTE: These products are for Research Use Only. Not for use with Humans.
Part # | Diameter (nm) | Length (nm) | Peak SPR Wave (nm) | Aspect Ratio | OD SPR (AU) | Peak LSPR Wave (nm) | OD LSPR (AU) | Nanorod Vol (nm3) | Nanorods /mL | Wt. conc (mg/ml) | Wt. % | PPM | Molarity (nM) | SPR Molar Ext. (M-1cm-1) | LSPR Molar Ext. (M-1cm-1) | Peak SPR accuracy (nm) | SPR Linewidth 80% (nm) |
D12M-10-1064 | 10 | 67 | 1064 | 6.7 | 50 | 510 | 10 | 5.02E+03 | 1.81E+13 | 1.75 | 0.18% | 1750 | 30.13 | 1.66E+09 | 3.32E+08 | 1022-1132 | 150 |
D12M-10-980 | 10 | 59 | 980 | 5.9 | 50 | 510 | 10 | 4.33E+03 | 2.09E+13 | 1.75 | 0.18% | 1750 | 34.91 | 1.43E+09 | 2.86E+08 | 965-1022 | 150 |
D12M-10-950 | 10 | 55 | 950 | 5.5 | 50 | 510 | 10 | 4.09E+03 | 2.22E+13 | 1.75 | 0.18% | 1750 | 37 | 1.35E+09 | 2.70E+08 | 925-965 | 150 |
D12M-10-900 | 10 | 50 | 900 | 5 | 50 | 510 | 10 | 3.68E+03 | 2.47E+13 | 1.75 | 0.18% | 1750 | 41.11 | 1.22E+09 | 2.43E+08 | 875-925 | 150 |
D12M-10-850 | 10 | 45 | 850 | 4.5 | 50 | 510 | 10 | 3.27E+03 | 2.78E+13 | 1.75 | 0.18% | 1750 | 46.25 | 1.08E+09 | 2.16E+08 | 829-875 | 100 |
D12M-10-808 | 10 | 41 | 808 | 4.1 | 50 | 510 | 10 | 2.93E+03 | 3.10E+13 | 1.75 | 0.18% | 1750 | 51.68 | 9.68E+08 | 1.94E+08 | 794-829 | 75 |
D12M-10-780 | 10 | 38 | 780 | 3.8 | 50 | 510 | 10 | 2.70E+03 | 3.36E+13 | 1.75 | 0.18% | 1750 | 56.06 | 8.92E+08 | 1.78E+08 | 765-794 | 65 |
Ntracker™ | Ntherapy™ | |
In batch size variation (10nm) | < 10% CV | < 10% CV |
Shape monodispersity | >95% nanorods | >95% nanorods |
Surface charge (zeta) | 0 mV | Amine +25mV, Carboxyl -5mV typ |
pH | 7.4 | 7.4 |
Residual Chemicals | Trace | Trace |
Toxicity | None | None |
Buffer | PBS | PBS |
SPR = Longitudinal SPR peak |
LSPR = Lower SPR peak |
Shape monodispersity (% rods) > 95% |
Aspect ratio variation = Peak SPR accuracy/96 |
All specs typical |
This product is delivered in PBS and is radiation sterilized |
Exact loading values are calculated |
Exact values are measured for each batch |
Part # | Diameter (nm) | Length (nm) | Peak SPR Wave (nm) | Aspect Ratio | OD SPR (AU) | Peak LSPR Wave (nm) | OD LSPR (AU) | Nanorod Vol (nm3) | Nanorods /mL | Wt. conc (mg/ml) | Wt. % | PPM | Molarity (nM) | SPR Molar Ext. (M-1cm-1) | LSPR Molar Ext. (M-1cm-1) | Peak SPR accuracy (nm) | SPR Linewidth 80% (nm) |
D12-10-1064 | 10 | 67 | 1064 | 6.7 | 50 | 510 | 10 | 5.02E+03 | 1.81E+13 | 1.75 | 0.18% | 1750 | 30.13 | 1.66E+09 | 3.32E+08 | 1022-1132 | 150 |
D12-10-980 | 10 | 59 | 980 | 5.9 | 50 | 510 | 10 | 4.33E+03 | 2.09E+13 | 1.75 | 0.18% | 1750 | 34.91 | 1.43E+09 | 2.86E+08 | 965-1022 | 150 |
D12-10-950 | 10 | 55 | 950 | 5.5 | 50 | 510 | 10 | 4.09E+03 | 2.22E+13 | 1.75 | 0.18% | 1750 | 37 | 1.35E+09 | 2.70E+08 | 925-965 | 150 |
D12-10-900 | 10 | 50 | 900 | 5 | 50 | 510 | 10 | 3.68E+03 | 2.47E+13 | 1.75 | 0.18% | 1750 | 41.11 | 1.22E+09 | 2.43E+08 | 875-925 | 150 |
D12-10-850 | 10 | 45 | 850 | 4.5 | 50 | 510 | 10 | 3.27E+03 | 2.78E+13 | 1.75 | 0.18% | 1750 | 46.25 | 1.08E+09 | 2.16E+08 | 829-875 | 100 |
D12-10-808 | 10 | 41 | 808 | 4.1 | 50 | 510 | 10 | 2.93E+03 | 3.10E+13 | 1.75 | 0.18% | 1750 | 51.68 | 9.68E+08 | 1.94E+08 | 794-829 | 75 |
D12-10-780 | 10 | 38 | 780 | 3.8 | 50 | 510 | 10 | 2.70E+03 | 3.36E+13 | 1.75 | 0.18% | 1750 | 56.06 | 8.92E+08 | 1.78E+08 | 765-794 | 65 |
Ntracker™ | Ntherapy™ | |
In batch size variation (10nm) | < 10% CV | < 10% CV |
Shape monodispersity | >95% nanorods | >95% nanorods |
Surface charge (zeta) | 0 mV | Amine +25mV, Carboxyl -5mV typ |
pH | 7.4 | 7.4 |
Residual Chemicals | Trace | Trace |
Toxicity | None | None |
Buffer | PBS | PBS |
SPR = Longitudinal SPR peak |
LSPR = Lower SPR peak |
Shape monodispersity (% rods) > 95% |
Aspect ratio variation = Peak SPR accuracy/96 |
All specs typical |
This product is delivered in PBS and is radiation sterilized |
Exact loading values are calculated |
Exact values are measured for each batch |
Part # | Diam (nm) | Length (nm) | Peak SPR Wave (nm) | Ex/Em (nm) | Wt. conc (mg/ml) (10-3) | Wt. % | PPM | Molarity (pM) | Spectrally Similar Dyes |
D16-10-1064 | 10 | 67 | 1064 | 777/794 | 1750.0 | 0.1750% | 1750 | 32.9 | IRDye 800 |
D16-10-980 | 10 | 59 | 980 | 777/794 | 1750.0 | 0.1750% | 1750 | 38.6 | IRDye 800 |
D16-10-850 | 10 | 45 | 850 | 777/794 | 1750.0 | 0.1750% | 1750 | 53.0 | IRDye 800 |
D16-10-808 | 10 | 41 | 808 | 777/794 | 1750.0 | 0.1750% | 1750 | 60.3 | IRDye 800 |
D16-10-780 | 10 | 38 | 780 | 754/776 | 1750.0 | 0.1750% | 1750 | 66.4 | Alexa Fluor 750 |
Functionalized gold nanoparticle with Nanopartz™ Ntherapy covalently bonded polymer. Thickness of polymer is less than the size variablity of the gold nanoparticle.
Conjugations (Ordering Abbreviation)
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Fluorophore Type | Excitation (nm) | Emission (nm) | Spectrally Similar Dyes |
800 | 788 | 808 | IRDye 800 |
750 | 750 | 773 | Alexa Fluor 750 |
Composition
These polymer coated gold nanorods are shipped in PBS with no measurable residual chemicals. This product is radiation sterilized and is ready for injection.
Custom Formulation
Popular CW laser wavelengths are available (780, 808, 850, 980, and 1064nm). Other sizes are special order. Please contact us.
Quantity
This product is available in 1mL and larger but in two different concentrations. The lower concentration is suited for mice, while the higher concentration is suitable for companion animals. For mice, the 1mL injection is suited for 5 injections. For orders larger than 100mL, or for orders amounting over 500mL per annum, please contact sales for quantity pricing.
Delivery
Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.
Conjugation
This product comes coated with a proprietary polymer to increase circulation times. Ntracker™ contains a non-reactive methyl termination. Ntherapy™ contains many choices for reactive terminations. Many popular in vivo conjugations are available.
Introductory Kits
Unfortunately there are no kits available for this product at this time.
Shelf Life/Storage Temperature
This product is guaranteed for one year and may be stored at room temperature.
Toxicity
These products are non cytotoxic.
Sterilization
This product is sterilized.
Certifications
This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis that includes the following information. We use NIST traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
Ntracker™ is based on extensive research in laboratory, preclinical, toxicology, and more recently clinical trials. The technology is based on patented, patent pending, and proprietary methods. Nanopartz™ has the capacity for up to 1kg of Ntracker™product per day in its audited ISO 9000 facility. Ntherapy™ offers the same technology as Ntracker™ with the added advantage of popular in vivo conjugations and customer specified oligo and antibody choices.
This product has been extensively tested in mice and companion animals.
Photoacoustic Images are provided by our partner Visualsonics Inc.
VisualSonics is the world leader in real-time, in vivo, high-resolution, micro-imaging systems, providing modalities specifically designed for preclinical research. VisualSonics has commercialized Vevo LAZR Technology, a state-of-the-art photoacoustic imaging system with inherent coregistration and capabilities for imaging gold nanorods in vivo. "Listen to the Light" - Photoacoustic imaging with the new Vevo LAZR System.
http://www.visualsonics.com/photoacoustics
Shallow-tissue modalities
Optical coherence tomography (OCT) [http://www.ncbi.nlm.nih.gov/pubmed/7585229]
OCT captures three-dimensional images from optical scattering media (e.g., biological tissue), and it sometimes can provide sub-micrometer resolution. However, the imaging depth in OCT is limited by optical scattering rather than absorption because scattering tends to attenuate and randomize the light. Depending on the wavelength of light, this technique can achieve imaging depths of up to 2 mm in most tissues. This technique has been used clinically for some applications such as eye examination and has been tested in vivo and ex vivo for cancer diagnosis. In order to generate sufficient contrast, the imaging agents for this modality need to have large scattering cross sections.
Photoacoustic tomography (PAT) [http://www.ncbi.nlm.nih.gov/pubmed/20049803]
PAT is a hybrid imaging modality that provides strong optical absorption contrast and high ultrasonic resolution. Because the spatial resolution beyond one optical transport mean free path (~1 mm) is determined by ultrasonic parameters, the maximum imaging depth and resolution of PAT are scalable when diffusive photons are available. One can greatly increase the penetration depth of PAT with near-infrared light because the optical absorption of hemoglobin and scattering of tissues are weak in this regime. Therefore, a proper combination of PAT with the right contrast agent can accurately detect and diagnose tumors. As in the case of OCT, the imaging depth depends on the wavelength of light, but the imaging depth (~30 mm) is higher than OCT. This modality is currently being evaluated in vivo. Additionally, contrast agents for PAT need to have large absorption cross sections.
Two-photon microscopy [http://www.ncbi.nlm.nih.gov/pubmed/2321027]
Two-photon microscopy is a fluorescence-based technique that offers images of living tissue up to ~1 mm in depth. It usually uses red-shifted light to minimize scattering in the tissue, and the background signal is strongly suppressed owing to multiphoton absorption. It is being tested in vivo. Recently, Nguyen and colleagues reported that surgery with molecular fluorescence imaging can be efficient for complete removal of tumors [http://www.ncbi.nlm.nih.gov/pubmed/20160097]. The results might suggest a breakthrough in the application of two-photon microscopy for molecular imaging to overcome the disadvantage of imaging depth.
Surface-enhanced Raman spectroscopy (SERS) imaging [http://www.ncbi.nlm.nih.gov/pubmed/9027306]
SERS utilizes the enhancement of Raman scattering by molecules adsorbed on surfaces of metal NPs. The increment can be as much as 10^14–10^15, hence Raman-active dyes placed on the surface of gold and silver colloids will exhibit greatly amplified Raman signals. The ability of gold colloids to easily conjugate with targeting ligands enables the detection of tumors in vivo using this technique [http://www.ncbi.nlm.nih.gov/pubmed/18157119, http://www.ncbi.nlm.nih.gov/pubmed/19666578].
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- Covalent bonds insure specificity, stability, long shelf life
- Buffer Stability - stable from pH 4-9
- No sodium azide
- No BSA
- Polymer coating insures no aggregation in high salts, reduced nonspecific binding
- Stable
- Well Characterized
- Customer can select buffer
- Customer can select gold nanoparticle type, size and/or SPR
- Loading of all ligands is optimized
- Customer can focus on research and not on Nanopartz™ expertise
- Quick turnaround
"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."
Stephan Link, PhD
Assistant Professor of Chemistry
Rice University
Pandia®
therapeutic technologies. ... There have been a number of studies conducted using Nanopartz AuNRs
that have ... for the IV injection of PEG-coated AuNRs for in vivo photothermal cancer ...
Selective fat removal using photothermal heating
wavelengths is important for therapeutic applications. ... Gold nanorods (GNRs) were procured from
Nanopartz™, specifically “Ntracker™ for in vivo Therapeutics” gold nanorods ...
In vivo real-time monitoring of nanoparticle clearance rate from blood circulation using high speed flow cytometry
obtained from Nanopartz Inc., Loveland, CO), had a similar ... Intrinsic therapeutic applications of
noble metal nanoparticles: past, present and future. ... In vivo biodistribution of nanoparticles. ...
Modelling and characterization of Photothermal effects assisted with Gold Nanorods in ex-vivo samples and in a murine model
Nanopartz). ... experimental results of photothermal effects in ex-vivo and in-vivo models. ... and Scott
A. Waldman, Applications of nanoparticles to diagnostics and therapeutics in colorectal ...
[CITATION] PASTGIN for diagnosing, monitoring, and treating breast cancer
Combined OCT and fluorescence imaging for cancer detection and therapeutic monitoring
Monitoring ... Fig. 2. Co-registered OCT/FMI imaging of intestinal polyps incubated with UEA-1
conjugated liposomes ex vivo. ... (B) Gold nanospheres (from Nanopartz, Inc., 50 nm in dia.) used ...
Gold nanorod photothermal therapy in a genetically engineered mouse model of soft tissue sarcoma
to xenograft ... demonstrated potential as multimodal diag- nostic and therapeutic agents in
vivo.7,9 ... coated gold NRs with longitudinal plasmon resonance at 810 (Nanopartz Inc.) were ...
[HTML] Plasmonic photothermal heating of intraperitoneal tumors through the use of an implanted near-infrared source
concentrated ... also acknowledge S. Malstrom from the Koch Institute Applied Therapeutics and
Whole ... Modeling parameters and schematics for NIR light simulations, ex vivo thermography of ...
Selective inactivation of enzymes conjugated to nanoparticles using tuned laser illumination
reactions and therapeutics. ... nanoparticles can be quantitatively detected ex vivo by atomic
absorption methods, and in vivo by CT ... Gold nanospheres were purchased from Nanopartz (cat ...