Nanopartz Functionalized Gold Nanoparticles

Nanopartz Gold Nanoparticles, conjugated specifically for in vitro applications, utilize the complete line of Accurate Spherical Gold Nanoparticles, Gold Nanorods, Microgold, Gold Nanowires, and Plasmonic Fluorophores conjugated via a proprietary covalent polymer bridge to the most popular in vitro terminations including PEG, alkyne, amine, azide, biotin, carboxyl, hydroxyl, methyl, maleimide, neutravidin, nhs, streptavidin, GSH, Protein A and G, Galactose, Thiol, and secondary antibodies. This family of products is well known for its resistant to temperature, salt, and pH, and for its exceptional loading capability. These conjugated particles can be used in a multitude of applications, including diagnostics, imaging, particularly in adverse environments. These particles are guaranteed not to aggregate. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. Typical volume is 1mL at a concentration of 50 OD. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the target is determined.

FREE - These products, like all of our products, are BSA Free, Sodium Azide Free, and Glycerol Free

image_0

 

Part #
Diameter (nm)
Peak SPR Wavelength (nm)
NPS/ml
Molarity (pM)
Moles
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Size Accuracy (+/- nm)
C11-1.8
1.8
n/a
4.25E+16
7.10E+07
7.09E-08
7.05E+05
7.05E+05
0.00E+00
<35%
0.1
C11-2.2
2.2
n/a
2.33E+16
3.90E+07
3.88E-08
1.29E+06
1.29E+06
0.00E+00
<25%
0.1
C11-3
3
n/a
9.19E+15
1.50E+07
1.53E-08
3.26E+06
3.26E+06
0.00E+00
<20%
0.1
C11-4
4
n/a
3.88E+15
6.50E+06
6.46E-09
7.74E+06
7.74E+06
0.00E+00
<20%
1
C11-5
5
512
1.99E+15
3.30E+06
3.31E-09
1.51E+07
1.51E+07
0.00E+00
<20%
2
C11-10
10
516
2.48E+14
4.10E+05
4.14E-10
1.21E+08
1.21E+08
0.00E+00
<15%
2
C11-15
15
518
7.35E+13
1.20E+05
1.23E-10
4.08E+08
4.08E+08
0.00E+00
<15%
2
C11-20
20
520
3.10E+13
5.20E+04
5.17E-11
9.67E+08
9.67E+08
0.00E+00
<10%
2
C11-25
25
521
1.59E+13
2.60E+04
2.65E-11
1.89E+09
1.89E+09
0.00E+00
<10%
2
C11-30
30
523
9.19E+12
1.50E+04
1.53E-11
3.26E+09
3.26E+09
0.00E+00
<6%
2
C11-35
35
526
5.79E+12
9.60E+03
9.65E-12
5.18E+09
5.18E+09
0.00E+00
<6%
2
C11-40
40
527
3.88E+12
6.50E+03
6.46E-12
7.74E+09
7.72E+09
2.24E+07
<4%
2
C11-45
45
529
2.72E+12
4.50E+03
4.54E-12
1.10E+10
1.08E+10
2.03E+08
<4%
2
C11-50
50
531
1.99E+12
3.30E+03
3.31E-12
1.51E+10
1.45E+10
5.88E+08
<4%
2
C11-55
55
533
1.49E+12
2.50E+03
2.49E-12
2.01E+10
1.88E+10
1.30E+09
<4%
2
C11-60
60
536
1.15E+12
1.90E+03
1.91E-12
2.61E+10
2.36E+10
2.48E+09
<4%
2
C11-65
65
539
9.04E+11
1.50E+03
1.51E-12
3.32E+10
2.89E+10
4.33E+09
<4%
2
C11-70
70
542
7.23E+11
1.20E+03
1.21E-12
4.15E+10
3.44E+10
7.09E+09
<4%
2
C11-75
75
545
5.88E+11
9.80E+02
9.80E-13
5.10E+10
4.00E+10
1.10E+10
<4%
2
C11-80
80
549
4.85E+11
8.10E+02
8.08E-13
6.19E+10
4.54E+10
1.65E+10
<4%
2
C11-85
85
553
4.04E+11
6.70E+02
6.73E-13
7.42E+10
5.03E+10
2.39E+10
<4%
2
C11-90
90
558
3.40E+11
5.70E+02
5.67E-13
8.81E+10
5.44E+10
3.37E+10
<4%
2
C11-95
95
563
2.89E+11
4.80E+02
4.82E-13
1.04E+11
5.72E+10
4.65E+10
<4%
2
C11-100
100
569
2.48E+11
4.10E+02
4.14E-13
1.21E+11
5.82E+10
6.27E+10
<4%
2
C11-150
150
612
7.35E+10
1.20E+02
1.23E-13
4.08E+11
0.00E+00
4.08E+11
<4%
10
C11-200
200
n/a
3.10E+10
5.20E+01
5.17E-14
9.67E+11
0.00E+00
9.67E+11
<4%
10
C11-500
500
n/a
1.99E+09
3.30E+00
3.31E-15
1.51E+13
0.00E+00
1.51E+13
<4%
50
C11-1000
1000
n/a
2.48E+08
4.10E-01
4.14E-16
1.21E+14
0.00E+00
1.21E+14
<4%
100
C11-1500
1500
n/a
7.35E+07
1.20E-01
1.23E-16
4.08E+14
0.00E+00
4.08E+14
<4%
100

 

Optical Density >= 50, 5 for sizes >=500nm
Wt conc = 2.5mg/mL, 1mL volume typical
Wt % = 0.25%
ppm = 2500
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
Shape monodispersity (% spheres) > 99.9%
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Part #
Diameter (nm)
Length (nm)
Peak SPR Wave (nm)
Aspect Ratio
OD SPR (AU)
Peak LSPR Wave (nm)
OD LSPR (AU)
Nanorods /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (nM)
Picomoles
SPR Molar Ext. (M-1cm-1)
LSPR Molar Ext. (M-1cm-1)

Peak SPR

(nm)

SPR Linewidth 80% (nm)
C12-5-808
5
21
808
4.2
50
510
10
2.39E+14
1750
0.18%
1750
398.72
3.99E+02
1.25E+08
2.51E+07
794-829
100
C12-5-780
5
19
780
3.8
50
510
10
2.67E+14
1750
0.18%
1750
444.72
4.45E+02
1.12E+08
2.25E+07
765-794
100
C12-5-700
5
15
700
3
50
510
10
3.47E+14
1750
0.18%
1750
578.14
5.78E+02
8.65E+07
1.73E+07
675-725
100
C12-10-2100
10
175
2100
17.5
50
510
10
6.73E+12
1750
0.18%
1750
11.21
1.12E+01
4.46E+09
8.92E+08
1900-2300
300
C12-10-1400
10
102
1400
10.2
50
510
10
1.17E+13
1750
0.18%
1750
19.47
1.95E+01
2.57E+09
5.14E+08
1300-1500
150
C12-10-1200
10
81
1200
8.1
50
510
10
1.48E+13
1750
0.18%
1750
24.67
2.47E+01
2.03E+09
4.05E+08
1132-1300
150
C12-10-1064
10
67
1064
6.7
50
510
10
1.81E+13
1750
0.18%
1750
30.13
3.01E+01
1.66E+09
3.32E+08
1022-1132
150
C12-10-980
10
59
980
5.9
50
510
10
2.09E+13
1750
0.18%
1750
34.91
3.49E+01
1.43E+09
2.86E+08
965-1022
150
C12-10-950
10
55
950
5.5
50
510
10
2.22E+13
1750
0.18%
1750
37
3.70E+01
1.35E+09
2.70E+08
925-965
150
C12-10-900
10
50
900
5
50
510
10
2.47E+13
1750
0.18%
1750
41.11
4.11E+01
1.22E+09
2.43E+08
875-925
150
C12-10-850
10
45
850
4.5
50
510
10
2.78E+13
1750
0.18%
1750
46.25
4.63E+01
1.08E+09
2.16E+08
829-875
100
C12-10-808
10
41
808
4.1
50
510
10
3.10E+13
1750
0.18%
1750
51.68
5.17E+01
9.68E+08
1.94E+08
794-829
75
C12-10-780
10
38
780
3.8
50
510
10
3.36E+13
1750
0.18%
1750
56.06
5.61E+01
8.92E+08
1.78E+08
765-794
65
C12-10-750
10
35
750
3.5
50
510
10
3.70E+13
1750
0.18%
1750
61.67
6.17E+01
8.11E+08
1.62E+08
725-765
50
C12-10-700
10
29
700
2.9
50
510
10
4.44E+13
1750
0.18%
1750
74
7.40E+01
6.76E+08
1.35E+08
675-725
40
C12-10-650
10
24
650
2.4
50
510
10
5.55E+13
1750
0.18%
1750
92.5
9.25E+01
5.41E+08
1.08E+08
625-675
40
C12-10-600
10
19
600
1.9
50
510
10
7.40E+13
1750
0.18%
1750
123.34
1.23E+02
4.05E+08
8.11E+07
575-625
40
C12-25-1400
25
245
1400
9.8
50
514
15
1.12E+12
2500
0.25%
2500
1.86
1.86E+00
2.69E+10
8.06E+09
1232-1500
150
C12-25-1064
25
137
1064
5.5
50
514
15
2.05E+12
2500
0.25%
2500
3.42
3.42E+00
1.46E+10
4.38E+09
1022-1232
150
C12-25-980
25
119
980
4.8
50
514
15
2.39E+12
2500
0.25%
2500
3.98
3.98E+00
1.26E+10
3.77E+09
965-1022
150
C12-25-950
25
102
950
4.1
50
514
15
2.82E+12
2500
0.25%
2500
4.7
4.70E+00
1.06E+10
3.19E+09
925-965
150
C12-25-900
25
96
900
3.8
50
514
15
3.01E+12
2500
0.25%
2500
5.02
5.02E+00
9.95E+09
2.99E+09
875-925
150
C12-25-850
25
93
850
3.7
50
514
15
3.12E+12
2500
0.25%
2500
5.2
5.20E+00
9.61E+09
2.88E+09
829-875
150
C12-25-808
25
90
808
3.6
50
514
15
3.24E+12
2500
0.25%
2500
5.39
5.39E+00
9.27E+09
2.78E+09
794-829
150
C12-25-780
25
87
780
3.5
50
514
15
3.36E+12
2500
0.25%
2500
5.6
5.60E+00
8.93E+09
2.68E+09
765-794
150
C12-25-750
25
85
750
3.4
50
514
15
3.45E+12
2500
0.25%
2500
5.75
5.75E+00
8.70E+09
2.61E+09
725-765
65
C12-25-700
25
75
700
3
50
514
15
3.96E+12
2500
0.25%
2500
6.61
6.61E+00
7.57E+09
2.27E+09
675-725
60
C12-25-650
25
71
650
2.8
50
514
15
4.22E+12
2500
0.25%
2500
7.03
7.03E+00
7.11E+09
2.13E+09
625-675
60
C12-25-600
25
57
600
2.3
50
514
15
5.43E+12
2500
0.25%
2500
9.05
9.05E+00
5.52E+09
1.66E+09
575-625
60
C12-25-550
25
34
550
1.4
50
514
15
1.01E+13
2500
0.25%
2500
16.91
1.69E+01
2.96E+09
8.87E+08
525-575
60
C12-40-1064
40
208
1064
5.2
50
520
20
5.30E+11
2500
0.25%
2500
0.88
8.84E-01
5.66E+10
2.26E+10
1022-1232
150
C12-40-980
40
180
980
4.5
50
520
20
6.19E+11
2500
0.25%
2500
1.03
1.03E+00
4.84E+10
1.94E+10
875-1022
150
C12-40-850
40
148
850
3.7
50
520
20
7.67E+11
2500
0.25%
2500
1.28
1.28E+00
3.91E+10
1.57E+10
829-875
150
C12-40-808
40
134
808
3.4
50
520
20
8.56E+11
2500
0.25%
2500
1.43
1.43E+00
3.51E+10
1.40E+10
794-829
150
C12-40-780
40
124
780
3.1
50
520
20
9.33E+11
2500
0.25%
2500
1.55
1.55E+00
3.22E+10
1.29E+10
765-794
150
C12-40-750
40
112
750
2.8
50
520
20
1.05E+12
2500
0.25%
2500
1.74
1.74E+00
2.87E+10
1.15E+10
725-765
150
C12-40-700
40
92
700
2.3
50
520
20
1.31E+12
2500
0.25%
2500
2.19
2.19E+00
2.29E+10
9.14E+09
675-725
60
C12-40-650
40
80
650
2
50
520
20
1.55E+12
2500
0.25%
2500
2.58
2.58E+00
1.94E+10
7.75E+09
625-675
60
C12-40-600
40
68
600
1.7
50
520
20
1.89E+12
2500
0.25%
2500
3.15
3.15E+00
1.59E+10
6.35E+09
575-625
60
C12-40-550
40
60
550
1.5
50
520
20
2.21E+12
2500
0.25%
2500
3.69
3.69E+00
1.36E+10
5.42E+09
525-575
60
C12-50-1064
50
245
1064
4.9
50
530
25
2.89E+11
2500
0.25%
2500
0.48
4.82E-01
1.04E+11
5.18E+10
980-1232
150
C12-50-808
50
145
808
2.9
50
530
25
5.15E+11
2500
0.25%
2500
0.86
8.58E-01
5.83E+10
2.91E+10
750-850
150
C12-50-700
50
110
700
2.2
50
530
25
7.08E+11
2500
0.25%
2500
1.18
1.18E+00
4.24E+10
2.12E+10
650-750
150
C12-50-600
50
100
600
2
50
530
25
7.93E+11
2500
0.25%
2500
1.32
1.32E+00
3.78E+10
1.89E+10
550-650
150
C12-70-750
70
120
750
1.7
50
530
25
3.49E+11
2500
0.25%
2500
0.58
5.81E-01
8.60E+10
4.30E+10
700-800
150
C12-70-650
70
105
650
1.5
50
530
25
4.13E+11
2500
0.25%
2500
0.69
6.88E-01
7.27E+10
3.63E+10
600-700
150
C12-70-550
70
91
550
1.3
50
530
25
4.98E+11
2500
0.25%
2500
0.83
8.30E-01
6.02E+10
3.01E+10
500-600
150

 

Optical Density >= 50, volume = 1mL
All specs typical. May vary batch to batch. Exact values are measured for each batch
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

Part #
Diameter (nm)
Length (nm)
Aspect Ratio
Peak SPR Wave (nm)
SPR OD (1cm)
Microgold /mL
moles
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
SPR Molar Ext. (M-1cm-1)
C13-500
75
500
6.7
510
0.25
6.18E+09
1.03E-14
250
0.03%
250
10.3
2.43E+07
C13-1000
100
1000
10
510
0.25
1.71E+09
2.85E-15
250
0.03%
250
2.85
8.78E+07

 

SPR = Transverse SPR peak
Longitudinal has no SPR peak
Shape monodispersity (% rods) > 95%
Size variation +/-10% (both dimensions)

 

 

Part #
Diameter (nm)
Length (nm)
Aspect Ratio
Peak SPR Wave (nm)
Nanowires Vol (nm3)
Nanowires /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
SPR Molar Ext. (M-1cm-1)
C14-1000
75
1000
13
510
4.31E+06
3.01E+09
250
0.03%
250
5.02
9.96E+06
C14-2000
75
2000
27
510
8.72E+06
1.49E+09
250
0.03%
250
2.48
2.02E+07
C14-4000
75
4000
53
510
1.76E+07
7.39E+08
250
0.03%
250
1.23
4.06E+07
C14-6000
75
6000
80
510
2.64E+07
4.91E+08
250
0.03%
250
0.82
6.10E+07
C14-10000
75
10000
133
510
4.40E+07
2.94E+08
250
0.03%
250
0.49
1.02E+08
C14-20000
100
20000
200
510
1.57E+08
8.27E+07
250
0.03%
250
0.14
3.63E+08
C14-40000
140
40000
286
510
6.15E+08
2.11E+07
250
0.03%
250
0.04
1.42E+09

 

SPR = Transverse SPR peak
Longitudinal has no SPR peak
Shape monodispersity (% rods) > 95%
Size variation +/-10% (both dimensions)

 

Functionalized Nanorod Block Invitro

 

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer

 

 

Loading Range (nm2)
Charge (mV)
Smaller particles Larger particles Smaller particles Larger particles
PEG
3
1
-27
-12
Alkyne
4
2
-25
-25
Amine
4
2
-20
20
Azide
4
2
-25
-25
Biotin
3
1.5
-25
-15
Carboxyl
4
2
-38
-45
Methyl
4
2
-27
-12
Streptavidin
0.01
0.01
-25
-10
Neutravidin
0.01
0.01
-25
-10
GSH (Glutathione)
3
1.5
-25
-15
Protein A
0.04
0.04
-25
-10
Protein G
0.04
0.04
-25
-10
Maleimide
4
2
-27
-12
NHS
3
1.5
-25
-15
Hydroxyl
4
2
-27
-12
Thiol
5
5
-30
-30
Galactose
2
2
-30
-30
Mutab
5
5
30
40
Secondary IgG
0.02
0.02
0.02
0.02

Composition

These nanoparticles are shipped in 18MEG DI water (default solution) with no measurable residual reactants.

Custom Formulation

Please contact us.

Quantity

This product is available in 1mL volumes and larger.

Introductory Kits

Please contact us.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a number of different covalent options.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

For post sterilization testing, please choose Sterilization Certification.

For endotoxin purification, choose Endotoxin Purified.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Spherical Gold Nanoparticles UV VIS

How much thickness does your proprietary capping agent add?

Anywhere from 1-5nm.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

No

What is the shelf life if I never open the package and keep refrigerated?

Many years.

Should I store in the refrigerator?

Yes.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

How does your polymer bridge compare to PEG?

It is superior in its resistance to salt, pH, and other chemicals.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

Enhancing sensitivity of surface plasmon resonance biosensors by functionalized gold nanoparticles: size matters

T Špringer, ML Ermini, B Špačková… - Analytical …, 2014 - ACS Publications
... resistivity, Direct-Q from Millipore). Gold Nanoparticles Spherical AuNPs
covalently functionalized with neutravidin via a 2 nm polymer bridge were
purchased from Nanopartz Inc., USA. The diameters of the gold ...

 

Physisorption of functionalized gold nanoparticles on AlGaN/GaN high electron mobility transistors for sensing applications

MS Makowski, S Kim, M Gaillard, D Janes… - Applied physics …, 2013 - aip.scitation.org
... used to measure electrical characteristics of physisorbed gold nanoparticles (Au NPs)
functionalized with alkanethiols ... linkers to the gate area of AlGaN/GaN HEMTs include patterning
gold at the ... Also, the effectiveness of the UV functionalization method is offset by the limited ...

 

[HTML] Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis …

PM Tiwari, E Eroglu, SS Bawage, K Vig, ME Miller… - Biomaterials, 2014 - Elsevier
... Carboxyl-polymer coated spherical GNPs (Nanopartz™, Loveland, CO, USA) 13 nm in size ... For
fluorescence microscopy, FITC conjugated VG-21 peptide was used for functionalization... GNPs
functionalized with FITC-conjugated VG-21 were used to study internalization of the ...

 

Wide‐field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy

S Patskovsky, E Bergeron, D Rioux… - Journal of …, 2015 - Wiley Online Library
... ON) were tethered to AuNPs (50 µg/mL, 100 nm diameter, Nanopartz, A11-100 ... Some examples
of experimental spectral curves for single 100 nm functionalized AuNPs are presented in ... shifted
for about 6 nm that could be explained by AuNPs functionalization and attachment to ...

 

Size dependence of Au NP-enhanced surface plasmon resonance based on differential phase measurement

S Zeng, X Yu, WC Law, Y Zhang, R Hu, XQ Dinh… - Sensors and Actuators B …, 2013 - Elsevier
... OD = 1.0) with diameters 40 nm, 60 nm, 70 nm and 80 nm were purchased from Nanopartz
Accurate. ... The system was left for a period of 20 min and then rinsed by DI water to confirm the
functionalization... A review on functionalized gold nanoparticles for biosensing applications. ...

 

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN Afrooz, IA Khan, SM Hussain… - … science & technology, 2013 - ACS Publications
... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs) in ... with 10 nm primary particle diameter were procured from Nanopartz
Inc. ... PA-SWNT Preparation To perform surface functionalization with pluronic acid (PA) F127 ...

 

Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

T Špringer, J Homola - Analytical and bioanalytical chemistry, 2012 - Springer
... a mixture of carboxy-terminated and hydroxy-terminated thiols were obtained from Nanopartz,
USA, at ... During the functionalization processes described above, the flow rate and the temperature
were set ... BSA was flowed along the surface of an SPR chip functionalized with Ab1 ...

 

Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles

M Mancuso, L Jiang, E Cesarman, D Erickson - Nanoscale, 2013 - pubs.rsc.org
... Gold and silver nanoparticle functionalizationGold and silver particles with average diameters
of 15 and 20 nm, respectively, were functionalized using thiol based chemistry using methods
described by others. ... Gold nanoparticles were purchased from Nanopartz (Loveland, CO ...

 

[HTML] Plasmonic enhancement of dye sensitized solar cells via a tailored size-distribution of chemically functionalized gold nanoparticles

C Andrei, E Lestini, S Crosbie, C de Frein, T O'Reilly… - PLoS …, 2014 - journals.plos.org
... particles with a tailored size distribution and commercially available (spherical) AuNPs Nanopartz,
wt 1.75 ... nm), with optically dispersing TiO 2 particles (Ø ∼190 nm) and functionalized with AuNPs ...
TiO 2 functionalisation with AuNPs) and Figure 4 (after the functionalization) it can ...

The manufacturing method for these nanoparticles are based on our proprietary method. This family of products have resulted in more than 100 publications and is the cornerstone of our product line. Other covalent polymer bridges are available as well. Please follow the menu to find options from 1 carbon to 30KDa length covalent polymer bridges.

 

 

  Nanopartz™ in vitro Gold Nanoparticles PEG Gold Nanoparticles
Stability (salt,pH,chemicals) High Medium
Monovalent Yes No
Nonspecific binding Very low Medium
Sterilization Yes No

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized

Example part number is C11-10-TAZ-DIH-50-1-CS-EP where:

C11 - Product family number for In Vitro Spherical Gold Nanoparticles. For Gold Nanorods, its C12 and so forth.

10 - Sphere diameter in nanometers. Other choices are 1.8 to 1500

TAZ - Covalent ligand, in this case Azide.

DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS

50 - Optical Density, in this case OD=50, optional 250

1 - Volume (mL). Other choices are 5mL, 10mL and more.

CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification.

EP - Endotoxin Purified - The product is endotoxin purified and certified.

How to Order

Ordering by scrolling down and selecting the options from the selection below.

 

Home
» C- Conjugated Gold Nanoparticles for In-Vitro
Conjugated Spherical Gold Nanoparticles for In-Vitro
Conjugated Spherical Gold Nanoparticles for In-Vitro
Part Number: C11
Options: (DIAMETER)-(FUNCTIONALIZATION)-(MONO)-(BUFFER)-(OD-mLs)-(VOL)-CS-EP-NC
Example Part Number: 30nm diameter spherical gold nanoparticle, carboxyl polymer, 18MEG DD DI Water, 50 OD (2.5mg), in 1mL, Endotoxin Purified, and 0.1mL Negative Control
C11-30-TC-DIH-50-1-EP-0.1NC
QUICK SPECS
Part #
Diameter (nm)
Peak SPR Wavelength (nm)
NPS/ml
Molarity (pM)
Moles
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Size Accuracy (+/- nm)
C11-1.8
1.8
n/a
4.25E+16
7.10E+07
7.09E-08
7.05E+05
7.05E+05
0.00E+00
<35%
0.1
C11-2.2
2.2
n/a
2.33E+16
3.90E+07
3.88E-08
1.29E+06
1.29E+06
0.00E+00
<25%
0.1
C11-3
3
n/a
9.19E+15
1.50E+07
1.53E-08
3.26E+06
3.26E+06
0.00E+00
<20%
0.1
C11-4
4
n/a
3.88E+15
6.50E+06
6.46E-09
7.74E+06
7.74E+06
0.00E+00
<20%
1
C11-5
5
512
1.99E+15
3.30E+06
3.31E-09
1.51E+07
1.51E+07
0.00E+00
<20%
2
C11-10
10
516
2.48E+14
4.10E+05
4.14E-10
1.21E+08
1.21E+08
0.00E+00
<15%
2
C11-15
15
518
7.35E+13
1.20E+05
1.23E-10
4.08E+08
4.08E+08
0.00E+00
<15%
2
C11-20
20
520
3.10E+13
5.20E+04
5.17E-11
9.67E+08
9.67E+08
0.00E+00
<10%
2
C11-25
25
521
1.59E+13
2.60E+04
2.65E-11
1.89E+09
1.89E+09
0.00E+00
<10%
2
C11-30
30
523
9.19E+12
1.50E+04
1.53E-11
3.26E+09
3.26E+09
0.00E+00
<6%
2
C11-35
35
526
5.79E+12
9.60E+03
9.65E-12
5.18E+09
5.18E+09
0.00E+00
<6%
2
C11-40
40
527
3.88E+12
6.50E+03
6.46E-12
7.74E+09
7.72E+09
2.24E+07
<4%
2
C11-45
45
529
2.72E+12
4.50E+03
4.54E-12
1.10E+10
1.08E+10
2.03E+08
<4%
2
C11-50
50
531
1.99E+12
3.30E+03
3.31E-12
1.51E+10
1.45E+10
5.88E+08
<4%
2
C11-55
55
533
1.49E+12
2.50E+03
2.49E-12
2.01E+10
1.88E+10
1.30E+09
<4%
2
C11-60
60
536
1.15E+12
1.90E+03
1.91E-12
2.61E+10
2.36E+10
2.48E+09
<4%
2
C11-65
65
539
9.04E+11
1.50E+03
1.51E-12
3.32E+10
2.89E+10
4.33E+09
<4%
2
C11-70
70
542
7.23E+11
1.20E+03
1.21E-12
4.15E+10
3.44E+10
7.09E+09
<4%
2
C11-75
75
545
5.88E+11
9.80E+02
9.80E-13
5.10E+10
4.00E+10
1.10E+10
<4%
2
C11-80
80
549
4.85E+11
8.10E+02
8.08E-13
6.19E+10
4.54E+10
1.65E+10
<4%
2
C11-85
85
553
4.04E+11
6.70E+02
6.73E-13
7.42E+10
5.03E+10
2.39E+10
<4%
2
C11-90
90
558
3.40E+11
5.70E+02
5.67E-13
8.81E+10
5.44E+10
3.37E+10
<4%
2
C11-95
95
563
2.89E+11
4.80E+02
4.82E-13
1.04E+11
5.72E+10
4.65E+10
<4%
2
C11-100
100
569
2.48E+11
4.10E+02
4.14E-13
1.21E+11
5.82E+10
6.27E+10
<4%
2
C11-150
150
612
7.35E+10
1.20E+02
1.23E-13
4.08E+11
0.00E+00
4.08E+11
<4%
10
C11-200
200
n/a
3.10E+10
5.20E+01
5.17E-14
9.67E+11
0.00E+00
9.67E+11
<4%
10
C11-500
500
n/a
1.99E+09
3.30E+00
3.31E-15
1.51E+13
0.00E+00
1.51E+13
<4%
50
C11-1000
1000
n/a
2.48E+08
4.10E-01
4.14E-16
1.21E+14
0.00E+00
1.21E+14
<4%
100
C11-1500
1500
n/a
7.35E+07
1.20E-01
1.23E-16
4.08E+14
0.00E+00
4.08E+14
<4%
100
Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$440.00
Conjugated Gold Nanorods for In-Vitro
Conjugated Gold Nanorods for In-Vitro
Part Number: C12
Options: (DIAM-SPR)-(FUNCTIONALIZATION)-(BUFFER)-(OD-mLs)-(VOL)-CS-EP-NC
Example Part Number: 10x45nm diameter gold nanorod with SPR=850nm, carboxyl polymer, 18MEG DD DI Water, 50 OD (2.5mg), in 1mL, Endotoxin Purified, and 0.1mL Negative Control
C12-10-850-TC-DIH-50-1-EP-0.1NC

QUICK SPECS

Part #
Diameter (nm)
Length (nm)
Peak SPR Wave (nm)
Aspect Ratio
OD SPR (AU)
Peak LSPR Wave (nm)
OD LSPR (AU)
Nanorods /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (nM)
Picomoles
SPR Molar Ext. (M-1cm-1)
LSPR Molar Ext. (M-1cm-1)

Peak SPR

(nm)

SPR Linewidth 80% (nm)
C12-5-808
5
21
808
4.2
50
510
10
2.39E+14
1750
0.18%
1750
398.72
3.99E+02
1.25E+08
2.51E+07
794-829
100
C12-5-780
5
19
780
3.8
50
510
10
2.67E+14
1750
0.18%
1750
444.72
4.45E+02
1.12E+08
2.25E+07
765-794
100
C12-5-700
5
15
700
3
50
510
10
3.47E+14
1750
0.18%
1750
578.14
5.78E+02
8.65E+07
1.73E+07
675-725
100
C12-10-2100
10
175
2100
17.5
50
510
10
6.73E+12
1750
0.18%
1750
11.21
1.12E+01
4.46E+09
8.92E+08
1900-2300
300
C12-10-1400
10
102
1400
10.2
50
510
10
1.17E+13
1750
0.18%
1750
19.47
1.95E+01
2.57E+09
5.14E+08
1300-1500
150
C12-10-1200
10
81
1200
8.1
50
510
10
1.48E+13
1750
0.18%
1750
24.67
2.47E+01
2.03E+09
4.05E+08
1132-1300
150
C12-10-1064
10
67
1064
6.7
50
510
10
1.81E+13
1750
0.18%
1750
30.13
3.01E+01
1.66E+09
3.32E+08
1022-1132
150
C12-10-980
10
59
980
5.9
50
510
10
2.09E+13
1750
0.18%
1750
34.91
3.49E+01
1.43E+09
2.86E+08
965-1022
150
C12-10-950
10
55
950
5.5
50
510
10
2.22E+13
1750
0.18%
1750
37
3.70E+01
1.35E+09
2.70E+08
925-965
150
C12-10-900
10
50
900
5
50
510
10
2.47E+13
1750
0.18%
1750
41.11
4.11E+01
1.22E+09
2.43E+08
875-925
150
C12-10-850
10
45
850
4.5
50
510
10
2.78E+13
1750
0.18%
1750
46.25
4.63E+01
1.08E+09
2.16E+08
829-875
100
C12-10-808
10
41
808
4.1
50
510
10
3.10E+13
1750
0.18%
1750
51.68
5.17E+01
9.68E+08
1.94E+08
794-829
75
C12-10-780
10
38
780
3.8
50
510
10
3.36E+13
1750
0.18%
1750
56.06
5.61E+01
8.92E+08
1.78E+08
765-794
65
C12-10-750
10
35
750
3.5
50
510
10
3.70E+13
1750
0.18%
1750
61.67
6.17E+01
8.11E+08
1.62E+08
725-765
50
C12-10-700
10
29
700
2.9
50
510
10
4.44E+13
1750
0.18%
1750
74
7.40E+01
6.76E+08
1.35E+08
675-725
40
C12-10-650
10
24
650
2.4
50
510
10
5.55E+13
1750
0.18%
1750
92.5
9.25E+01
5.41E+08
1.08E+08
625-675
40
C12-10-600
10
19
600
1.9
50
510
10
7.40E+13
1750
0.18%
1750
123.34
1.23E+02
4.05E+08
8.11E+07
575-625
40
C12-25-1400
25
245
1400
9.8
50
514
15
1.12E+12
2500
0.25%
2500
1.86
1.86E+00
2.69E+10
8.06E+09
1232-1500
150
C12-25-1064
25
137
1064
5.5
50
514
15
2.05E+12
2500
0.25%
2500
3.42
3.42E+00
1.46E+10
4.38E+09
1022-1232
150
C12-25-980
25
119
980
4.8
50
514
15
2.39E+12
2500
0.25%
2500
3.98
3.98E+00
1.26E+10
3.77E+09
965-1022
150
C12-25-950
25
102
950
4.1
50
514
15
2.82E+12
2500
0.25%
2500
4.7
4.70E+00
1.06E+10
3.19E+09
925-965
150
C12-25-900
25
96
900
3.8
50
514
15
3.01E+12
2500
0.25%
2500
5.02
5.02E+00
9.95E+09
2.99E+09
875-925
150
C12-25-850
25
93
850
3.7
50
514
15
3.12E+12
2500
0.25%
2500
5.2
5.20E+00
9.61E+09
2.88E+09
829-875
150
C12-25-808
25
90
808
3.6
50
514
15
3.24E+12
2500
0.25%
2500
5.39
5.39E+00
9.27E+09
2.78E+09
794-829
150
C12-25-780
25
87
780
3.5
50
514
15
3.36E+12
2500
0.25%
2500
5.6
5.60E+00
8.93E+09
2.68E+09
765-794
150
C12-25-750
25
85
750
3.4
50
514
15
3.45E+12
2500
0.25%
2500
5.75
5.75E+00
8.70E+09
2.61E+09
725-765
65
C12-25-700
25
75
700
3
50
514
15
3.96E+12
2500
0.25%
2500
6.61
6.61E+00
7.57E+09
2.27E+09
675-725
60
C12-25-650
25
71
650
2.8
50
514
15
4.22E+12
2500
0.25%
2500
7.03
7.03E+00
7.11E+09
2.13E+09
625-675
60
C12-25-600
25
57
600
2.3
50
514
15
5.43E+12
2500
0.25%
2500
9.05
9.05E+00
5.52E+09
1.66E+09
575-625
60
C12-25-550
25
34
550
1.4
50
514
15
1.01E+13
2500
0.25%
2500
16.91
1.69E+01
2.96E+09
8.87E+08
525-575
60
C12-40-1064
40
208
850
5.2
50
520
20
5.30E+11
2500
0.25%
2500
0.88
8.84E-01
5.66E+10
2.26E+10
1022-1232
150
C12-40-980
40
180
850
4.5
50
520
20
6.19E+11
2500
0.25%
2500
1.03
1.03E+00
4.84E+10
1.94E+10
875-1022
150
C12-40-850
40
148
850
3.7
50
520
20
7.67E+11
2500
0.25%
2500
1.28
1.28E+00
3.91E+10
1.57E+10
829-875
150
C12-40-808
40
134
808
3.4
50
520
20
8.56E+11
2500
0.25%
2500
1.43
1.43E+00
3.51E+10
1.40E+10
794-829
150
C12-40-780
40
124
780
3.1
50
520
20
9.33E+11
2500
0.25%
2500
1.55
1.55E+00
3.22E+10
1.29E+10
765-794
150
C12-40-750
40
112
750
2.8
50
520
20
1.05E+12
2500
0.25%
2500
1.74
1.74E+00
2.87E+10
1.15E+10
725-765
150
C12-40-700
40
92
700
2.3
50
520
20
1.31E+12
2500
0.25%
2500
2.19
2.19E+00
2.29E+10
9.14E+09
675-725
60
C12-40-650
40
80
650
2
50
520
20
1.55E+12
2500
0.25%
2500
2.58
2.58E+00
1.94E+10
7.75E+09
625-675
60
C12-40-600
40
68
600
1.7
50
520
20
1.89E+12
2500
0.25%
2500
3.15
3.15E+00
1.59E+10
6.35E+09
575-625
60
C12-40-550
40
60
550
1.5
50
520
20
2.21E+12
2500
0.25%
2500
3.69
3.69E+00
1.36E+10
5.42E+09
525-575
60
C12-50-1064
50
245
1064
4.9
50
530
25
2.89E+11
2500
0.25%
2500
0.48
4.82E-01
1.04E+11
5.18E+10
980-1232
150
C12-50-808
50
145
808
2.9
50
530
25
5.15E+11
2500
0.25%
2500
0.86
8.58E-01
5.83E+10
2.91E+10
750-850
150
C12-50-700
50
110
700
2.2
50
530
25
7.08E+11
2500
0.25%
2500
1.18
1.18E+00
4.24E+10
2.12E+10
650-750
150
C12-50-600
50
100
600
2
50
530
25
7.93E+11
2500
0.25%
2500
1.32
1.32E+00
3.78E+10
1.89E+10
550-650
150
C12-70-750
70
120
750
1.7
50
530
25
3.49E+11
2500
0.25%
2500
0.58
5.81E-01
8.60E+10
4.30E+10
700-800
150
C12-70-650
70
105
650
1.5
50
530
25
4.13E+11
2500
0.25%
2500
0.69
6.88E-01
7.27E+10
3.63E+10
600-700
150
C12-70-550
70
91
550
1.3
50
530
25
4.98E+11
2500
0.25%
2500
0.83
8.30E-01
6.02E+10
3.01E+10
500-600
150
DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg for nanorods >=25nm, 1.75mg for nanorods 5nm and 10nm in diameter
Price$480.00
Conjugated Microgold for In-Vitro
Conjugated Microgold for In-Vitro
Part Number: C13
Options: (DIAM/LENGTH)-(FUNCTIONALIZATION)-(BUFFER)-(wt.)-(VOL)-CS-EP-NC
Example Part Number: 75nm diameter, 500nm length microgold, carboxyl polymer, 18MEG DD DI Water, 0.25mg, in 1mL, Endotoxin Purified, and 0.25mg Negative Control
C13-75/500-TC-DIH-0.25-1-EP-0.25NC

QUICK SPECS

Part #
Diameter (nm)
Length (nm)
Aspect Ratio
Peak SPR Wave (nm)
SPR OD (1cm)
Microgold /mL
moles
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
SPR Molar Ext. (M-1cm-1)
C13-500
75
500
6.7
510
0.25
6.18E+09
1.03E-14
250
0.03%
250
10.3
2.43E+07
C13-1000
100
1000
10
510
0.25
1.71E+09
2.85E-15
250
0.03%
250
2.85
8.78E+07
Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
Price$480.00
Conjugated Gold Nanowires for In-Vitro
Conjugated Gold Nanowires for In-Vitro
Part Number: C14
Options: (DIAM/LENGTH)-(FUNCTIONALIZATION)-(BUFFER)-(wt.)-(VOL)-CS-EP-NC
Example Part Number: 75nm diameter, 1000nm length microgold, carboxyl polymer, 18MEG DD DI Water, 0.25mg, in 1mL, Endotoxin Purified, and 0.25mg Negative Control
C14-75/1000-TC-DIH-0.25-1-EP-0.25NC

QUICK SPECS

Part #
Diameter (nm)
Length (nm)
Aspect Ratio
Peak SPR Wave (nm)
Nanowires Vol (nm3)
Nanowires /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
SPR Molar Ext. (M-1cm-1)
C14-1000
75
1000
13
510
4.31E+06
3.01E+09
250
0.03%
250
5.02
9.96E+06
C14-2000
75
2000
27
510
8.72E+06
1.49E+09
250
0.03%
250
2.48
2.02E+07
C14-4000
75
4000
53
510
1.76E+07
7.39E+08
250
0.03%
250
1.23
4.06E+07
C14-6000
75
6000
80
510
2.64E+07
4.91E+08
250
0.03%
250
0.82
6.10E+07
C14-10000
75
10000
133
510
4.40E+07
2.94E+08
250
0.03%
250
0.49
1.02E+08
C14-20000
100
20000
200
510
1.57E+08
8.27E+07
250
0.03%
250
0.14
3.63E+08
C14-40000
140
40000
286
510
6.15E+08
2.11E+07
250
0.03%
250
0.04
1.42E+09

 

Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
Price$480.00