Fluorophore Labeled Gold Nanoparticles for Cell Uptake

Nanopartz has developed a gold nanoparticle construct that has shown efficient cell uptake. These gold nanoparticles are spherical and nanorod shaped and can be imaged by either confocal fluorescence or electron microscopy. Most importantly, they are large enough that they don't need to be grown through silver or gold growth methods after the fact as post treatments are known to destroy cell ultrastructure. These products show the potential for drug and oligo delivery to cells. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Every product is in stock (99%) and is shipped same day. This product comes one concentration, OD=50 (2.5mg/mL) in 18MEG DI water (default), purified of residual reactants to <0.1%.

Cell Uptake Fluorophore Labeled GNPs

 

Part #
Diameter (nm)
Peak SPR Wavelength (nm)
NPS/ml
Molarity (pM)
Moles
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Size Accuracy (+/- nm)
PCU11-1.8
1.8
n/a
4.25E+16
7.10E+07
7.09E-08
7.05E+05
7.05E+05
0.00E+00
<35%
0.1
PCU11-2.2
2.2
n/a
2.33E+16
3.90E+07
3.88E-08
1.29E+06
1.29E+06
0.00E+00
<25%
0.1
PCU11-3
3
n/a
9.19E+15
1.50E+07
1.53E-08
3.26E+06
3.26E+06
0.00E+00
<20%
0.1
PCU11-4
4
n/a
3.88E+15
6.50E+06
6.46E-09
7.74E+06
7.74E+06
0.00E+00
<20%
1
PCU11-5
5
512
1.99E+15
3.30E+06
3.31E-09
1.51E+07
1.51E+07
0.00E+00
<20%
2
PCU11-10
10
516
2.48E+14
4.10E+05
4.14E-10
1.21E+08
1.21E+08
0.00E+00
<15%
2
PCU11-15
15
518
7.35E+13
1.20E+05
1.23E-10
4.08E+08
4.08E+08
0.00E+00
<15%
2
PCU11-20
20
520
3.10E+13
5.20E+04
5.17E-11
9.67E+08
9.67E+08
0.00E+00
<10%
2
PCU11-25
25
521
1.59E+13
2.60E+04
2.65E-11
1.89E+09
1.89E+09
0.00E+00
<10%
2
PCU11-30
30
523
9.19E+12
1.50E+04
1.53E-11
3.26E+09
3.26E+09
0.00E+00
<6%
2
PCU11-35
35
526
5.79E+12
9.60E+03
9.65E-12
5.18E+09
5.18E+09
0.00E+00
<6%
2
PCU11-40
40
527
3.88E+12
6.50E+03
6.46E-12
7.74E+09
7.72E+09
2.24E+07
<4%
2
PCU11-45
45
529
2.72E+12
4.50E+03
4.54E-12
1.10E+10
1.08E+10
2.03E+08
<4%
2
PCU11-50
50
531
1.99E+12
3.30E+03
3.31E-12
1.51E+10
1.45E+10
5.88E+08
<4%
2
PCU11-55
55
533
1.49E+12
2.50E+03
2.49E-12
2.01E+10
1.88E+10
1.30E+09
<4%
2
PCU11-60
60
536
1.15E+12
1.90E+03
1.91E-12
2.61E+10
2.36E+10
2.48E+09
<4%
2
PCU11-65
65
539
9.04E+11
1.50E+03
1.51E-12
3.32E+10
2.89E+10
4.33E+09
<4%
2
PCU11-70
70
542
7.23E+11
1.20E+03
1.21E-12
4.15E+10
3.44E+10
7.09E+09
<4%
2
PCU11-75
75
545
5.88E+11
9.80E+02
9.80E-13
5.10E+10
4.00E+10
1.10E+10
<4%
2
PCU11-80
80
549
4.85E+11
8.10E+02
8.08E-13
6.19E+10
4.54E+10
1.65E+10
<4%
2
PCU11-85
85
553
4.04E+11
6.70E+02
6.73E-13
7.42E+10
5.03E+10
2.39E+10
<4%
2
PCU11-90
90
558
3.40E+11
5.70E+02
5.67E-13
8.81E+10
5.44E+10
3.37E+10
<4%
2
PCU11-95
95
563
2.89E+11
4.80E+02
4.82E-13
1.04E+11
5.72E+10
4.65E+10
<4%
2
PCU11-100
100
569
2.48E+11
4.10E+02
4.14E-13
1.21E+11
5.82E+10
6.27E+10
<4%
2

 

Optical Density >= 50
Wt conc = 2.5mg/mL, 1mL volume typical
Wt % = 0.25%
ppm = 2500
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
Shape monodispersity (% spheres) > 99.9%
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Part #
Diameter (nm)
Length (nm)
Peak SPR Wave (nm)
Aspect Ratio
OD SPR (AU)
Peak LSPR Wave (nm)
OD LSPR (AU)
Nanorods /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (nM)
Picomoles
SPR Molar Ext. (M-1cm-1)
LSPR Molar Ext. (M-1cm-1)

Peak SPR

(nm)

SPR Linewidth 80% (nm)
PCU12-5-808
5
21
808
4.2
50
510
10
2.39E+14
1750
0.18%
1750
398.72
3.99E+02
1.25E+08
2.51E+07
794-829
100
PCU12-5-780
5
19
780
3.8
50
510
10
2.67E+14
1750
0.18%
1750
444.72
4.45E+02
1.12E+08
2.25E+07
765-794
100
PCU12-5-700
5
15
700
3
50
510
10
3.47E+14
1750
0.18%
1750
578.14
5.78E+02
8.65E+07
1.73E+07
675-725
100
PCU12-10-2100
10
175
2100
17.5
50
510
10
6.73E+12
1750
0.18%
1750
11.21
1.12E+01
4.46E+09
8.92E+08
1900-2300
300
PCU12-10-1400
10
102
1400
10.2
50
510
10
1.17E+13
1750
0.18%
1750
19.47
1.95E+01
2.57E+09
5.14E+08
1300-1500
150
PCU12-10-1200
10
81
1200
8.1
50
510
10
1.48E+13
1750
0.18%
1750
24.67
2.47E+01
2.03E+09
4.05E+08
1132-1300
150
PCU12-10-1064
10
67
1064
6.7
50
510
10
1.81E+13
1750
0.18%
1750
30.13
3.01E+01
1.66E+09
3.32E+08
1022-1132
150
PCU12-10-980
10
59
980
5.9
50
510
10
2.09E+13
1750
0.18%
1750
34.91
3.49E+01
1.43E+09
2.86E+08
965-1022
150
PCU12-10-950
10
55
950
5.5
50
510
10
2.22E+13
1750
0.18%
1750
37
3.70E+01
1.35E+09
2.70E+08
925-965
150
PCU12-10-900
10
50
900
5
50
510
10
2.47E+13
1750
0.18%
1750
41.11
4.11E+01
1.22E+09
2.43E+08
875-925
150
PCU12-10-850
10
45
850
4.5
50
510
10
2.78E+13
1750
0.18%
1750
46.25
4.63E+01
1.08E+09
2.16E+08
829-875
100
PCU12-10-808
10
41
808
4.1
50
510
10
3.10E+13
1750
0.18%
1750
51.68
5.17E+01
9.68E+08
1.94E+08
794-829
75
PCU12-10-780
10
38
780
3.8
50
510
10
3.36E+13
1750
0.18%
1750
56.06
5.61E+01
8.92E+08
1.78E+08
765-794
65
PCU12-10-750
10
35
750
3.5
50
510
10
3.70E+13
1750
0.18%
1750
61.67
6.17E+01
8.11E+08
1.62E+08
725-765
50
PCU12-10-700
10
29
700
2.9
50
510
10
4.44E+13
1750
0.18%
1750
74
7.40E+01
6.76E+08
1.35E+08
675-725
40
PCU12-10-650
10
24
650
2.4
50
510
10
5.55E+13
1750
0.18%
1750
92.5
9.25E+01
5.41E+08
1.08E+08
625-675
40
PCU12-10-600
10
19
600
1.9
50
510
10
7.40E+13
1750
0.18%
1750
123.34
1.23E+02
4.05E+08
8.11E+07
575-625
40
PCU12-25-1400
25
245
1400
9.8
50
514
15
1.12E+12
2500
0.25%
2500
1.86
1.86E+00
2.69E+10
8.06E+09
1232-1500
150
PCU12-25-1064
25
137
1064
5.5
50
514
15
2.05E+12
2500
0.25%
2500
3.42
3.42E+00
1.46E+10
4.38E+09
1022-1232
150
PCU12-25-980
25
119
980
4.8
50
514
15
2.39E+12
2500
0.25%
2500
3.98
3.98E+00
1.26E+10
3.77E+09
965-1022
150
PCU12-25-950
25
102
950
4.1
50
514
15
2.82E+12
2500
0.25%
2500
4.7
4.70E+00
1.06E+10
3.19E+09
925-965
150
PCU12-25-900
25
96
900
3.8
50
514
15
3.01E+12
2500
0.25%
2500
5.02
5.02E+00
9.95E+09
2.99E+09
875-925
150
PCU12-25-850
25
93
850
3.7
50
514
15
3.12E+12
2500
0.25%
2500
5.2
5.20E+00
9.61E+09
2.88E+09
829-875
150
PCU12-25-808
25
90
808
3.6
50
514
15
3.24E+12
2500
0.25%
2500
5.39
5.39E+00
9.27E+09
2.78E+09
794-829
150
PCU12-25-780
25
87
780
3.5
50
514
15
3.36E+12
2500
0.25%
2500
5.6
5.60E+00
8.93E+09
2.68E+09
765-794
150
PCU12-25-750
25
85
750
3.4
50
514
15
3.45E+12
2500
0.25%
2500
5.75
5.75E+00
8.70E+09
2.61E+09
725-765
65
PCU12-25-700
25
75
700
3
50
514
15
3.96E+12
2500
0.25%
2500
6.61
6.61E+00
7.57E+09
2.27E+09
675-725
60
PCU12-25-650
25
71
650
2.8
50
514
15
4.22E+12
2500
0.25%
2500
7.03
7.03E+00
7.11E+09
2.13E+09
625-675
60
PCU12-25-600
25
57
600
2.3
50
514
15
5.43E+12
2500
0.25%
2500
9.05
9.05E+00
5.52E+09
1.66E+09
575-625
60
PCU12-25-550
25
34
550
1.4
50
514
15
1.01E+13
2500
0.25%
2500
16.91
1.69E+01
2.96E+09
8.87E+08
525-575
60
PCU12-40-1064
40
208
850
5.2
50
520
20
5.30E+11
2500
0.25%
2500
0.88
8.84E-01
5.66E+10
2.26E+10
1022-1232
150
PCU12-40-980
40
180
850
4.5
50
520
20
6.19E+11
2500
0.25%
2500
1.03
1.03E+00
4.84E+10
1.94E+10
875-1022
150
PCU12-40-850
40
148
850
3.7
50
520
20
7.67E+11
2500
0.25%
2500
1.28
1.28E+00
3.91E+10
1.57E+10
829-875
150
PCU12-40-808
40
134
808
3.4
50
520
20
8.56E+11
2500
0.25%
2500
1.43
1.43E+00
3.51E+10
1.40E+10
794-829
150
PCU12-40-780
40
124
780
3.1
50
520
20
9.33E+11
2500
0.25%
2500
1.55
1.55E+00
3.22E+10
1.29E+10
765-794
150
PCU12-40-750
40
112
750
2.8
50
520
20
1.05E+12
2500
0.25%
2500
1.74
1.74E+00
2.87E+10
1.15E+10
725-765
150
PCU12-40-700
40
92
700
2.3
50
520
20
1.31E+12
2500
0.25%
2500
2.19
2.19E+00
2.29E+10
9.14E+09
675-725
60
PCU12-40-650
40
80
650
2
50
520
20
1.55E+12
2500
0.25%
2500
2.58
2.58E+00
1.94E+10
7.75E+09
625-675
60
PCU12-40-600
40
68
600
1.7
50
520
20
1.89E+12
2500
0.25%
2500
3.15
3.15E+00
1.59E+10
6.35E+09
575-625
60
PCU12-40-550
40
60
550
1.5
50
520
20
2.21E+12
2500
0.25%
2500
3.69
3.69E+00
1.36E+10
5.42E+09
525-575
60
PCU12-50-1064
50
245
1064
4.9
50
530
25
2.89E+11
2500
0.25%
2500
0.48
4.82E-01
1.04E+11
5.18E+10
980-1232
150
PCU12-50-808
50
145
808
2.9
50
530
25
5.15E+11
2500
0.25%
2500
0.86
8.58E-01
5.83E+10
2.91E+10
750-850
150
PCU12-50-700
50
110
700
2.2
50
530
25
7.08E+11
2500
0.25%
2500
1.18
1.18E+00
4.24E+10
2.12E+10
650-750
150
PCU12-50-600
50
100
600
2
50
530
25
7.93E+11
2500
0.25%
2500
1.32
1.32E+00
3.78E+10
1.89E+10
550-650
150
PCU12-70-750
70
120
750
1.7
50
530
25
3.49E+11
2500
0.25%
2500
0.58
5.81E-01
8.60E+10
4.30E+10
700-800
150
PCU12-70-650
70
105
650
1.5
50
530
25
4.13E+11
2500
0.25%
2500
0.69
6.88E-01
7.27E+10
3.63E+10
600-700
150
PCU12-70-550
70
91
550
1.3
50
530
25
4.98E+11
2500
0.25%
2500
0.83
8.30E-01
6.02E+10
3.01E+10
500-600
150

 

Optical Density >= 50, volume = 1mL
All specs typical. May vary batch to batch. Exact values are measured for each batch
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Ligand Description Zeta Space to Add fluorophores or Payload Covalent?

Cell uptake

Efficiency

CU Nanopartz Proprietary Cell Uptake +10 Yes Yes High
MUTAB Positive charged, covalent attached (11-Mercaptoundecyl)-N N N-trimethylammonium bromide +35 No Yes Medium
MTAB Positive charged, covalent attached (16-mercaptohexadecyl)tri-methylammonium bromide +40 No Yes Medium

 

Wavelength Name Excitation (nm) Emission (nm) Ext. Coef QY
405 AF405 402 421    
  Dy405 400 420    
488 FITC 494 518 70000  
  Dy488 493 518 70000  
  AF488 490 525    
514 NPZ514 520 540    
532 Rhodamine 535 575    
550 Cy3 555 570 150000 0.31
  Dy550 562 576    
  AF555 555 580    
600 Cy3.5 591 604 116000 0.35
  Dy594 593 618    
  AF594 590 617    
  Texas Red 592 614    
650 Cy5 646 662 250000 0.2
  Dy 650 652 672    
  AF647 650 665    

 

Composition

These gold nanoparticles are shipped in  DI water with no measurable residual reactants.

Custom Formulation

Diameters 1.8nm to 3nm.  Larger sizes may reduce cell uptake efficiency. Please contact us.

Quantity

This product is available in 1mL volumes and larger. For orders larger than 1L, or for orders amounting over 10L per annum, please contact sales for quantity pricing.

Introductory Kits

None are currently available.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a number of Frag' IgG functionalizations with a complete range of fluorophore choices.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Spherical Gold Nanoparticles UV VIS

Gold Nanorods UV VIS

 

What makes these nanoparticles so efficient at cell uptake?

It is well known that the strongly positive MUTAB and MTAB ligands lead to efficient cell uptake. Unfortunately because of their construct, there is no way to add antibodies, oligos, fluorophore or any other payloads. The Nanopartz Cell Uptake polymer resolves that issue.

Why are you able to get high efficiency cell uptake with such large gold nanoparticles?

Two reasons: 1) the monodispersity of our gold nanoparticles and 2) the ligand design construct. Our ligand design has reduced the overall size of the construct, making it more efficient at nucleus uptake.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

"We were previously making our own gold nanoparticles using the commonly used technique of citrate reduction of Au(III). The colloids generated are broadly polydisperse, and we were concerned that our application of these particles in immuno-gold labeling of cells for optical coherence microscopy (OCM) would be utilizing only the large diameter tail of the distribution. We purchased the sampler package of NanoPartz gold spheres (30 nm, 50 nm, 70 nm, 90 nm) and examined each size carefully with dynamic light scattering, OCM, and spectrophotometry. Our measurements indicated diameters a couple nanometers larger than specifications (a result of measurements on the fully hydrated state?), and remarkable monodispersity - within specs. It is clear to us now that our early immuno-gold results were dominated by the largest diameter spheres of our early colloids. We are delighted by the NanoPartz monodisperse preparations and recommend them strongly!"

Dr. Richard C. Haskell 
Professor of Physics & Director of Physics Clinic 
Physics Department 
Harvey Mudd College



"The scientists at Nanopartz have developed a line of colloidal gold nanorods with extremely high monodispersity in size, shape, and mass. The surfactant layer is highly stable and readily displaced for biofunctionalization. In every test, in vitro and in vivo, these materials have exceeded our expectations."


Geoffrey A. von Maltzahn
NSF and Whitaker Graduate Fellow
Harvard-MIT Division of Health Sciences and Technology
Laboratory for Multiscale Regenerative Technologies

 

Intracellular localization and kinetics of uptake and clearance of gold nanoparticles in primary hepatic cells
CJ DETZEL, W LENG, PJ VIKESLAND… - Nano Life, 2012 - World Scientific
… HeLa1 and prostate cancer cells10 at 6000 and 40 000 particles per cell, respectively … 3(b)].
Hepatocyte uptake of UnmodiŊed-Nanopartz AuNPs increased from 2% of the available
nanoparticles … 3(a)]. Over a 24h period UnmodiŊed-Nano- partz particles were taken up at 4 …

Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages
MM Janát-Amsbury, A Ray, CM Peterson… - European Journal of …, 2011 - Elsevier
… in diameter and rod-shaped particles with reported dimensions of 10 × 45 nm) were purchased
from Nanopartz, Inc … Before used, gold nanoparticle samples were incubated with cell culture
medium for 1 week to evaluate for potential contaminations that adversely affect cells …

Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres
A Malugin, H Ghandehari - Journal of Applied Toxicology: An …, 2010 - Wiley Online Library
… Plain, PEGylated spherical and PEGylated rod GNPs were obtained from Nanopartz (Salt Lake
City, UT, USA) and … Cells were kept in the logarithmic phase of cell growth for the duration of
experiments. For the uptake studies PC-3 cells were incubated with GNPs at 37 °C in 24 …

Cold plasma accelerates the uptake of gold nanoparticles into glioblastoma cells
X Cheng, K Rajjoub, J Sherman… - Plasma Processes …, 2015 - Wiley Online Library
… in the amount of nanoparticles absorbed in the U87 cell line when the cells are treated … the
acceleration effect: plasma reaction with the AuNPs, and plasma stimulation of the cell membrane.
The AuNPs purchased from Nanopartz were not coated with any ligands or polymers …

C60-SIMS imaging of nanoparticles within mammalian cells
AN Bloom, H Tian, N Winograd - Biointerphases, 2016 - avs.scitation.org
… Imaging Probes: Cellular Interaction And Intracellular Magnetic Iron Oxide Nanoparticles Uptake
In Brain … tubules via TOF-SIMS tandem mass spectrometry imaging of transfected cells Corryn
E … β-D-glucose/sucrose-coated silver nanoparticles depends on cell type, nanoparticles …

Label-free visualization of nilotinib-functionalized gold nanoparticles within single mammalian cells by C60- SIMS imaging
AN Bloom, H Tian, C Schoen, N Winograd - Analytical and bioanalytical …, 2017 - Springer
… Exposure to nilotinib-functionalized gold nanoparticles. The 20-nm cell uptake
polymer/nilotinib-functionalized gold nanospheres (Nanopartz, Loveland, CO, USA)
were studied within cells without modification. After 24 h of growth …

Uptake of gold nanoparticles by intestinal epithelial cells: impact of particle size on their absorption, accumulation, and toxicity
M Yao, L He, DJ McClements… - Journal of agricultural and …, 2015 - ACS Publications
… AuNPs with different diameters (15, 50, and 100 nm) were purchased from Nanopartz (Loveland,
CO, USA) … To visualize the uptake of AuNPs in intestinal epithelial cells, suspensions of AuNPs
(5 μg/mL; 15, 50, or 100 nm) were applied on Caco-2 cell monolayers …


Variation in the uptake of nanoparticles by monolayer cultured cells using high resolution MeV ion beam imaging
Y Tao, Z Mi, SK Vanga, CB Chen, AA Bettiol… - Nuclear Instruments and …, 2015 - Elsevier
… In this paper we describe the preliminary work carried out on ectocervical cells incubated with
50 nm gold nanoparticles, where the nanoparticles within the cell were imaged … Gold nanoparticles
(Au NP) with an average diameter of 50 nm (Nanopartz Inc.) were prepared …

Gold Nanoparticle‐Loaded Neural Stem Cells for Photothermal Ablation of Cancer
K Schnarr, R Mooney, Y Weng, D Zhao… - Advanced …, 2013 - Wiley Online Library
… bromide (MUTAB) coated gold nanorods (AuNRs) with longitudinal plasmon resonances at 810nm
were supplied by Nanopartz, a division of … The media was then removed and the cells were washed,
trypsinized and centrifuged, and a the cell pellets were fixed with 2 …
Cited by 51 Related articles All 5 versions

 

The manufacturing method for these nanoparticles are based on our proprietary method. This invention allowed the first use of a reducing agent to be used at room temperature. Unlike citrate which is only a reducing agent at the boiling temperature of water - thereby quickly making non-monodisperse gold nanoparticles, our reducing agent works by slowing building nanoparticles from seed, atom by atom, over the course of up to hours. The result is a highly monodisperse product with unrivaled spherical shapes.

  Nanopartz™Accurate™ Spherical Gold Nanoparticles Other Technologies
Monodispersity High (<2%) Low (>15%)
Size Accuracy High (+/-2nm) Low
Linewidths Narrow Broad
Storage lifetime Years 6 months at 4C
Ability to ligand exchange Easy More difficult

 

  • Highly accurate and monodisperse in size, shape, and batch to batch uniformity.
    • Monodisperse sizes and shapes leads to better standards for electron microscopy and other diagnostics.
  • Accurate to +/- 2 nm in diameter.
    • More accurate sizing makes for better SPR matching for Surface Enhanced Raman Labels.
  • Size monodispersity better than 4% CV for many sizes.
    • Monodisperse sizes and shapes leads to better Resonance Light Scattering labels in sensitivity and multiplexing
  • Greater than 99.9% spheres.
  • Carboxylic acid capping agent is reduced to < 0.1% without adverse effects to the spheres.
    • Reduced number of additives improves binding to antibodies, receptors, ligands and DNA.
  • Extremely stable.  Shelf life is greater than one year.  May be stored at room temperature for extended periods.
  • The light scattering power of a 60 nm gold particle is equivalent to ~5 X 105 fluorescein molecules.
  • Accurate™ SGNPs do not photobleach.
    • No photobleaching means better diagnostic labels with the ability to archive.
  • Light scatter can be detected by the unaided eye at particle concentrations as low as 10-15 M (femtomolar) in suspension and as low as 0.005 particles/m2 on a transparent surface.
    • 105 better scattering power than fluorescein means lower limits of detection with simpler instrumentation.
  • Individual gold nanoparticles can be seen by eye in an inexpensive student microscope with simple dark field illumination allowing for very sensitive and inexpensive particle counting assays.
  • Different sized particles scatter different colors of light and therefore can be used as multicolor multiplexed labels.
  • Facile conjugations allow for coating of antibodies, receptors, ligands, DNA without affecting their light scattering properties.
  • Slightly negatively charged (reduced aggregation).
  • Facile synthesis.  Gold chemistry is well known.
  • No known cytotoxicity

Example part number is PCU11-10-488-DIH-50-1-CS-EP where:

PCU11 - Product family number for Nanopartz Cell Uptake

10 - Sphere diameter in nanometers.

488 - FITC Fluorophore

DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS

50 - Optical Density, in this case OD=50

1 - Volume (mL). Other choices are 5mL.

CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification.

EP - Endotoxin Purified - The product is endotoxin purified and certified.

How to Order

Ordering by scrolling down and selecting the options from the selection below.

 

Home » P - Applications » PCU- Cell Uptake
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PCU11 Spherical Gold Nanoparticles for Cell Uptake
PCU11 Spherical Gold Nanoparticles for Cell Uptake
Part Number: PCU11-(SIZE)-(FUNCTIONALIZATION)-(FLUOROPHORE)-(BUFFER)-(OD-mLs)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL) Example part number for a 30nm diameter sphere, NCU (Nanopartz Cell Uptake Polymer)-550nm fluorophore, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: PCU11-30-NCU-550-DIH-50-1
 

UV-VIS

 Note:NCU (Nanopartz Cell Uptake Polymer)
MUTAB (11-Mercaptoundecyl)-N N N-trimethylammonium bromide)
MTAB (16-mercaptohexadecyl)tri-methylammonium bromide)
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Negative Control Using the same source gold nanoparticle lot, this product provides a non-reactive negative control 50 OD in either 0.1mL or 1mL volumes. Use this product for confirmation of your active ligand (ie amine, etc.)
0.1NC (0.1mL Negative Control) (+$100.00)
1NC (1mL Negative Control) (+$480.00)
Price: $520.00
PCU12 Gold Nanorods for Cell Uptake
PCU12 Gold Nanorods for Cell Uptake
Part Number: PCU12-(SIZE)-(FUNCTIONALIZATION)-(FLUOROPHORE)-(BUFFER)-(OD-mLs)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL) Example part number for a 10nm diameter rod with a 808nm SPR, NCU (Nanopartz Cell Uptake Polymer)-550nm fluorophore, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: PCU12-10-808-NCU-550-DIH-50-1
 DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
 Note:NCU (Nanopartz Cell Uptake Polymer)
MUTAB (11-Mercaptoundecyl)-N N N-trimethylammonium bromide)
MTAB (16-mercaptohexadecyl)tri-methylammonium bromide)
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Negative Control Using the same source gold nanoparticle lot, this product provides a non-reactive negative control 50 OD in either 0.1mL or 1mL volumes. Use this product for confirmation of your active ligand (ie amine, etc.)
0.1NC (0.1mL Negative Control) (+$100.00)
1NC (1mL Negative Control) (+$480.00)
Price: $520.00