Nanopartz™ One-Step Gold Nanoparticle to Antibody Conjugation Kit

Nanopartz™ One-Step™ Antibody Conjugated Gold Nanoparticles utilizes Nanopartz™ proprietary technology that allows users to covalently bind Nanopartz™ gold nanoparticle to your antibodies in less than 10 minutes. All that is necessary is a simple centrifuge for purification. This product includes choices for the full line of monodisperse spherical and nanorod familes of products. The result is a covalently attached antibody to the construct of choice. The NHS active group will quickly and efficiently bind to any primary amine. Unlike carboxyl chemistry that requires multiple steps and EDC chemicals, this product requires simple addition of the antibody to the gold nanoparticles.

image_3

 

Part #

CS11-

Diameter (nm)
Peak SPR Wavelength (nm)
NPS/ml
Molarity (pM)
Moles
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Size Accuracy (+/- nm)
1.8
1.8
n/a
4.25E+16
7.10E+07
7.09E-08
7.05E+05
7.05E+05
0.00E+00
<35%
0.1
2.2
2.2
n/a
2.33E+16
3.90E+07
3.88E-08
1.29E+06
1.29E+06
0.00E+00
<25%
0.1
3
3
n/a
9.19E+15
1.50E+07
1.53E-08
3.26E+06
3.26E+06
0.00E+00
<20%
0.1
4
4
n/a
3.88E+15
6.50E+06
6.46E-09
7.74E+06
7.74E+06
0.00E+00
<20%
1
5
5
512
1.99E+15
3.30E+06
3.31E-09
1.51E+07
1.51E+07
0.00E+00
<20%
2
10
10
516
2.48E+14
4.10E+05
4.14E-10
1.21E+08
1.21E+08
0.00E+00
<15%
2
15
15
518
7.35E+13
1.20E+05
1.23E-10
4.08E+08
4.08E+08
0.00E+00
<15%
2
20
20
520
3.10E+13
5.20E+04
5.17E-11
9.67E+08
9.67E+08
0.00E+00
<10%
2
25
25
521
1.59E+13
2.60E+04
2.65E-11
1.89E+09
1.89E+09
0.00E+00
<10%
2
30
30
523
9.19E+12
1.50E+04
1.53E-11
3.26E+09
3.26E+09
0.00E+00
<6%
2
35
35
526
5.79E+12
9.60E+03
9.65E-12
5.18E+09
5.18E+09
0.00E+00
<6%
2
40
40
527
3.88E+12
6.50E+03
6.46E-12
7.74E+09
7.72E+09
2.24E+07
<4%
2
45
45
529
2.72E+12
4.50E+03
4.54E-12
1.10E+10
1.08E+10
2.03E+08
<4%
2
50
50
531
1.99E+12
3.30E+03
3.31E-12
1.51E+10
1.45E+10
5.88E+08
<4%
2
55
55
533
1.49E+12
2.50E+03
2.49E-12
2.01E+10
1.88E+10
1.30E+09
<4%
2
60
60
536
1.15E+12
1.90E+03
1.91E-12
2.61E+10
2.36E+10
2.48E+09
<4%
2
65
65
539
9.04E+11
1.50E+03
1.51E-12
3.32E+10
2.89E+10
4.33E+09
<4%
2
70
70
542
7.23E+11
1.20E+03
1.21E-12
4.15E+10
3.44E+10
7.09E+09
<4%
2
75
75
545
5.88E+11
9.80E+02
9.80E-13
5.10E+10
4.00E+10
1.10E+10
<4%
2
80
80
549
4.85E+11
8.10E+02
8.08E-13
6.19E+10
4.54E+10
1.65E+10
<4%
2
85
85
553
4.04E+11
6.70E+02
6.73E-13
7.42E+10
5.03E+10
2.39E+10
<4%
2
90
90
558
3.40E+11
5.70E+02
5.67E-13
8.81E+10
5.44E+10
3.37E+10
<4%
2
95
95
563
2.89E+11
4.80E+02
4.82E-13
1.04E+11
5.72E+10
4.65E+10
<4%
2
100
100
569
2.48E+11
4.10E+02
4.14E-13
1.21E+11
5.82E+10
6.27E+10
<4%
2
150
150
612
7.35E+10
1.20E+02
1.23E-13
4.08E+11
0.00E+00
4.08E+11
<4%
10
200
200
n/a
3.10E+10
5.20E+01
5.17E-14
9.67E+11
0.00E+00
9.67E+11
<4%
10
500
500
n/a
1.99E+09
3.30E+00
3.31E-15
1.51E+13
0.00E+00
1.51E+13
<4%
50
1000
1000
n/a
2.48E+08
4.10E-01
4.14E-16
1.21E+14
0.00E+00
1.21E+14
<4%
100
1500
1500
n/a
7.35E+07
1.20E-01
1.23E-16
4.08E+14
0.00E+00
4.08E+14
<4%
100

 

Optical Density >= 50, 5 for sizes >=500nm
Wt conc = 2.5mg/mL, 1mL volume typical
Wt % = 0.25%
ppm = 2500
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
Shape monodispersity (% spheres) > 99.9%
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Part #

CS12-

Diameter (nm)
Length (nm)
Peak SPR Wave (nm)
Aspect Ratio
OD SPR (AU)
Peak LSPR Wave (nm)
OD LSPR (AU)
Nanorods /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (nM)
Picomoles
SPR Molar Ext. (M-1cm-1)
LSPR Molar Ext. (M-1cm-1)

Peak SPR

(nm)

SPR Linewidth 80% (nm)
5-808
5
21
808
4.2
50
510
10
2.39E+14
1750
0.18%
1750
398.72
3.99E+02
1.25E+08
2.51E+07
794-829
100
5-780
5
19
780
3.8
50
510
10
2.67E+14
1750
0.18%
1750
444.72
4.45E+02
1.12E+08
2.25E+07
765-794
100
5-700
5
15
700
3
50
510
10
3.47E+14
1750
0.18%
1750
578.14
5.78E+02
8.65E+07
1.73E+07
675-725
100
10-2100
10
175
2100
17.5
50
510
10
6.73E+12
1750
0.18%
1750
11.21
1.12E+01
4.46E+09
8.92E+08
1900-2300
300
10-1400
10
102
1400
10.2
50
510
10
1.17E+13
1750
0.18%
1750
19.47
1.95E+01
2.57E+09
5.14E+08
1300-1500
150
10-1200
10
81
1200
8.1
50
510
10
1.48E+13
1750
0.18%
1750
24.67
2.47E+01
2.03E+09
4.05E+08
1132-1300
150
10-1064
10
67
1064
6.7
50
510
10
1.81E+13
1750
0.18%
1750
30.13
3.01E+01
1.66E+09
3.32E+08
1022-1132
150
10-980
10
59
980
5.9
50
510
10
2.09E+13
1750
0.18%
1750
34.91
3.49E+01
1.43E+09
2.86E+08
965-1022
150
10-950
10
55
950
5.5
50
510
10
2.22E+13
1750
0.18%
1750
37
3.70E+01
1.35E+09
2.70E+08
925-965
150
10-900
10
50
900
5
50
510
10
2.47E+13
1750
0.18%
1750
41.11
4.11E+01
1.22E+09
2.43E+08
875-925
150
10-850
10
45
850
4.5
50
510
10
2.78E+13
1750
0.18%
1750
46.25
4.63E+01
1.08E+09
2.16E+08
829-875
100
10-808
10
41
808
4.1
50
510
10
3.10E+13
1750
0.18%
1750
51.68
5.17E+01
9.68E+08
1.94E+08
794-829
75
10-780
10
38
780
3.8
50
510
10
3.36E+13
1750
0.18%
1750
56.06
5.61E+01
8.92E+08
1.78E+08
765-794
65
10-750
10
35
750
3.5
50
510
10
3.70E+13
1750
0.18%
1750
61.67
6.17E+01
8.11E+08
1.62E+08
725-765
50
10-700
10
29
700
2.9
50
510
10
4.44E+13
1750
0.18%
1750
74
7.40E+01
6.76E+08
1.35E+08
675-725
40
10-650
10
24
650
2.4
50
510
10
5.55E+13
1750
0.18%
1750
92.5
9.25E+01
5.41E+08
1.08E+08
625-675
40
10-600
10
19
600
1.9
50
510
10
7.40E+13
1750
0.18%
1750
123.34
1.23E+02
4.05E+08
8.11E+07
575-625
40
25-1400
25
245
1400
9.8
50
514
15
1.12E+12
2500
0.25%
2500
1.86
1.86E+00
2.69E+10
8.06E+09
1232-1500
150
25-1064
25
137
1064
5.5
50
514
15
2.05E+12
2500
0.25%
2500
3.42
3.42E+00
1.46E+10
4.38E+09
1022-1232
150
25-980
25
119
980
4.8
50
514
15
2.39E+12
2500
0.25%
2500
3.98
3.98E+00
1.26E+10
3.77E+09
965-1022
150
25-950
25
102
950
4.1
50
514
15
2.82E+12
2500
0.25%
2500
4.7
4.70E+00
1.06E+10
3.19E+09
925-965
150
25-900
25
96
900
3.8
50
514
15
3.01E+12
2500
0.25%
2500
5.02
5.02E+00
9.95E+09
2.99E+09
875-925
150
25-850
25
93
850
3.7
50
514
15
3.12E+12
2500
0.25%
2500
5.2
5.20E+00
9.61E+09
2.88E+09
829-875
150
25-808
25
90
808
3.6
50
514
15
3.24E+12
2500
0.25%
2500
5.39
5.39E+00
9.27E+09
2.78E+09
794-829
150
25-780
25
87
780
3.5
50
514
15
3.36E+12
2500
0.25%
2500
5.6
5.60E+00
8.93E+09
2.68E+09
765-794
150
25-750
25
85
750
3.4
50
514
15
3.45E+12
2500
0.25%
2500
5.75
5.75E+00
8.70E+09
2.61E+09
725-765
65
25-700
25
75
700
3
50
514
15
3.96E+12
2500
0.25%
2500
6.61
6.61E+00
7.57E+09
2.27E+09
675-725
60
25-650
25
71
650
2.8
50
514
15
4.22E+12
2500
0.25%
2500
7.03
7.03E+00
7.11E+09
2.13E+09
625-675
60
25-600
25
57
600
2.3
50
514
15
5.43E+12
2500
0.25%
2500
9.05
9.05E+00
5.52E+09
1.66E+09
575-625
60
25-550
25
34
550
1.4
50
514
15
1.01E+13
2500
0.25%
2500
16.91
1.69E+01
2.96E+09
8.87E+08
525-575
60
40-1064
40
208
850
5.2
50
520
20
5.30E+11
2500
0.25%
2500
0.88
8.84E-01
5.66E+10
2.26E+10
1022-1232
150
40-980
40
180
850
4.5
50
520
20
6.19E+11
2500
0.25%
2500
1.03
1.03E+00
4.84E+10
1.94E+10
875-1022
150
40-850
40
148
850
3.7
50
520
20
7.67E+11
2500
0.25%
2500
1.28
1.28E+00
3.91E+10
1.57E+10
829-875
150
40-808
40
134
808
3.4
50
520
20
8.56E+11
2500
0.25%
2500
1.43
1.43E+00
3.51E+10
1.40E+10
794-829
150
40-780
40
124
780
3.1
50
520
20
9.33E+11
2500
0.25%
2500
1.55
1.55E+00
3.22E+10
1.29E+10
765-794
150
40-750
40
112
750
2.8
50
520
20
1.05E+12
2500
0.25%
2500
1.74
1.74E+00
2.87E+10
1.15E+10
725-765
150
40-700
40
92
700
2.3
50
520
20
1.31E+12
2500
0.25%
2500
2.19
2.19E+00
2.29E+10
9.14E+09
675-725
60
40-650
40
80
650
2
50
520
20
1.55E+12
2500
0.25%
2500
2.58
2.58E+00
1.94E+10
7.75E+09
625-675
60
40-600
40
68
600
1.7
50
520
20
1.89E+12
2500
0.25%
2500
3.15
3.15E+00
1.59E+10
6.35E+09
575-625
60
40-550
40
60
550
1.5
50
520
20
2.21E+12
2500
0.25%
2500
3.69
3.69E+00
1.36E+10
5.42E+09
525-575
60
50-1064
50
245
1064
4.9
50
530
25
2.89E+11
2500
0.25%
2500
0.48
4.82E-01
1.04E+11
5.18E+10
980-1232
150
50-808
50
145
808
2.9
50
530
25
5.15E+11
2500
0.25%
2500
0.86
8.58E-01
5.83E+10
2.91E+10
750-850
150
50-700
50
110
700
2.2
50
530
25
7.08E+11
2500
0.25%
2500
1.18
1.18E+00
4.24E+10
2.12E+10
650-750
150
50-600
50
100
600
2
50
530
25
7.93E+11
2500
0.25%
2500
1.32
1.32E+00
3.78E+10
1.89E+10
550-650
150
70-750
70
120
750
1.7
50
530
25
3.49E+11
2500
0.25%
2500
0.58
5.81E-01
8.60E+10
4.30E+10
700-800
150
70-650
70
105
650
1.5
50
530
25
4.13E+11
2500
0.25%
2500
0.69
6.88E-01
7.27E+10
3.63E+10
600-700
150
70-550
70
91
550
1.3
50
530
25
4.98E+11
2500
0.25%
2500
0.83
8.30E-01
6.02E+10
3.01E+10
500-600
150

 

Optical Density >= 50, volume = 1mL
All specs typical. May vary batch to batch. Exact values are measured for each batch
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

One Step Gold Nanoparticle Functionalization

 

One Step Nanopartz™ covalently bonded polymer. Thickness of polymer is 1-2nm.

 

 

Loading Range (nm2)
Charge (mV)
Conugation Smaller particles Larger particles Smaller particles Larger particles
One Step
4
2
-25
-25

Composition

These nanoparticles are shipped dry with no measurable residual reactants.

Custom Formulation

Please contact us.

Quantity

This product is available in amounts to be diluted to 1mL volumes and larger.

Introductory Kits

Please contact us.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a covalent NHS polymer.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

For post sterilization testing, please choose Sterilization Certification.

For endotoxin purification, choose Endotoxin Purified.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Spherical Gold Nanoparticles UV VIS

How much thickness does your proprietary ligand add?

Anywhere from 1-2nm.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

What is the shelf life if I never open the package and keep refrigerated?

Many years.

Should I store in the refrigerator?

Yes.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

How does your polymer bridge compare to PEG?

It is superior in its resistance to salt, pH, and other chemicals.

How easy is your kit to use?

Simply add your antibody to the provided gold nanoparticles. If lyophilized, add 1 mL of PBS. Vortex for 30 minutes. Centrifuge twice at the recommended speeds. That simple!

But when I've used adsorbed technology in the past, salt and other additives that came with the antibodies made my gold nanoparticle turn blue and aggregate. Will that happen here?

No.

How long will the resultant product last?

That all depends on the antibody and the sterilitity of your antibody and your process. For high quality and highly characterized product, we recommend you have us perform the conjugation.

Can I choose any buffer?

Just about.

Your saying that I will see an SPR shift upon conjugation, and no shift if there is no conjugation?

Absolutely.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

A smartphone platform for the quantification of vitamin D levels

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Vitamin D deficiency has been linked to a number of diseases and adverse outcomes 
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[PDF] Immunoassay on Cotton Yarn for Low-Cost Diagnostics

GZ Zhou, R Safaviah, X Mao, D Juncker - 14th International Conference on …, 2010 - rsc.org
... The dAb is conjugated to 44 nm Au colloids (Nanopartz Inc, Loveland, CO, USA) according to
a published ... b) Scanned image of the yarns that de- tects CRP concentration from 1 µg/ml to 320
µg ... The one-step assay emulates a lateral flow strip, where two types of yarns are used. ...
 

[CITATION] Conjugating Carboxyl-Polymer Spherical Gold Nanoparticles to Antibodies Kit

P Number - proteins, 2009
 

Competitive surface-enhanced Raman scattering assay for the 1, 25-dihydroxy metabolite of vitamin D 3

EJ Dufek, B Ehlert, MC Granger, TM Sandrock… - Analyst, 2010 - pubs.rsc.org
... Gold nanoparticles (GNP; 70 nm diameter) were purchased from Nanopartz; these particles have
a size ... procedure used for RIA, which uses one solid phase extraction column and one elution
step ... ν s (NO 2 ), the strongest feature in each spectrum, against the log of the 1,25-(OH ...
 

Self-illuminative cascade-reaction-driven anticancer therapeutic cassettes made of cooperatively interactive nanocomplexes

WC Song, SW Shin, KS Park, MS Jang, JH Choi… - Colloids and Surfaces B: …, 2015 - Elsevier
... no. A12-25-808, Nanopartz, Loveland, CO, USA) was centrifuged (5000 × g, 5 min) to remove
excess CTAB ... in a prostate cancer model, a cell viability test was conducted via WST-1 assay
(Fig. ... The PC3 prostate cell line which is one of the most fatal male cancer species over the ...
 

Development of gold nanorod lateral flow test for quantitative multi-analyte detection

M Venkataramasubramani, L Tang - … Conference 2009, 15–17 May 2009, …, 2009 - Springer
... Rapid diagnostic assays have been in use for decades in the clinical and medical sector with
the pregnancy test strip as one of the first ... Fig. 1 TEM image of gold nanorods with peak, axial
size: 10 nm, longitu- dinal: 35 nm (TEM data is courtesy of Nanopartz... 1. Xiulan S et al. ...

 

The manufacturing method for these nanoparticles are based on our proprietary method. This family of products have resulted in more than 100 publications and is the cornerstone of our product line.

 

 

  Nanopartz™ One Step Gold Nanoparticles Others
Stability (salt,pH,chemicals) High Medium
Monovalent Yes No
Nonspecific binding Very low Medium
Sterilization Yes No

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized

Example part number is CS11-30-550-5-0.1 where:

CS11 - Product family number for One Step Spherical Gold Nanoparticles. For Gold Nanorods, its CS12.

30 - Sphere diameter in nanometers. Other choices are 1.8 to 1500

550 - Optional Fluorophore

5 - Product is split into 5 tubes, approx. 0.5mg in each

0.1 - Negative Control. A non reactive nanoparticle from the same lot is inclued separately in 0.1mL solution

How to Order

Ordering by scrolling down and selecting the options from the selection below.

 

Home
» CS- One Step Conjugations
CS11
CS11
One Step Spherical Gold Nanoparticles
Description: Spherical Gold Nanoparticles, Covalent NHS Polymer bridge, 2.5mg, Dry, instructions and reagents
Part Number: CS11-(DIAMETER)-(FlUOROPHORE)-(SPLIT 5)-(NEGATIVE CONTROL)
Example part number: 30nm diameter sphere with a 550nm fluorophore, 0.1mL negative control, at 2.5mg, dry would be: CS11-30-550-M-0.1
Optional Fluorophore: Choose an additional attached fluorophore from 400nm to 800nm
Optional Split 5: A regular order is split into 5 separate tubes
Optional Negative Control: Negative Control uses the same source and covalent polymer bridge as your one step choice but replaces the NHS with a non reactive terminal group
 

UV-VIS

 Additional Fluorophore tag from 400nm to 800nm
Optional Split 5 A regular sized order is split into 5 separate tubes. For example a 2.5mg order is split into 5 separate 0.5mg tubes.
Split 5 tubes (+$40.00)
 Option: This negative control comes in a separate microtube - it uses the same source and covalent polymer bridge as your one step choice but replaces the NHS with a non reactive terminal group. OD=50, so 0.25mg in the 0.1mL size and 2.5mg in the 1mL size
Price$480.00
CS12
CS12
One Step Gold Nanorods
Description: Nanopartz Gold Nanorods, Covalent NHS Polymer bridge, 1.75mg for 10nm diameter nanorods, 2.5mg for 25, 40, and 50nm diameter nanorods, except 0.25mg for 25nm diameter nanorods with SPRs>980nm, and 0.175mg for 10nm nanorods with SPRs>1064nm. Dry, instructions and reagents
Part Number: CS12-(DIAMETER-SPR)-(FlUOROPHORE)-(SPLIT 5)-(NEGATIVE CONTROL)
Example part number: 10nm diameter nanorod with an 850nm absorption peak with a 550nm fluorophore, 0.1mL negative control, at 1.75mg, dry would be: CS12-10-850-550-0.1
Optional Fluorophore: Choose an additional attached fluorophore from 400nm to 800nm
Optional Split 5: A regular order is split into 5 separate tubes
Optional Negative Control: Negative Control uses the same source and covalent polymer bridge as your one step choice but replaces the NHS with a non reactive terminal group
 DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
 Additional Fluorophore tag from 400nm to 800nm
Optional Split 5 A regular sized order is split into 5 separate tubes. For example a 2.5mg order is split into 5 separate 0.5mg tubes.
Split 5 tubes (+$40.00)
 Option: This negative control comes in a separate microtube - it uses the same source and covalent polymer bridge as your one step choice but replaces the NHS with a non reactive terminal group. OD=50, so 0.25mg in the 0.1mL size and 2.5mg in the 1mL size
Price$480.00