Conjugated Package

Nanopartz™ One-Step Antibody Conjugated Gold Nanoparticles

Nanopartz™ One-Step Antibody Conjugated Gold Nanoparticles utilizes Nanopartz™ proprietary technology that allows users to covalently bind Nanopartz™ gold nanoparticle to your antibodies in less than 10 minutes. All that is necessary is a simple centrifuge for purification. This product includes choices for the full line of monodisperse spherical and nanorod familes, as well as our plasmonic fluorophores and Ramanprobes. The result is a covalently attached antibody to the construct of choice. The NHS active group will quickly and efficiently bind to any primary amine. Unlike carboxyl chemistry that requires multiple steps and EDC chemicals, this product requires simple addition of the antibody to the gold nanoparticles.

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One Step Spherical Gold Nanoparticles
One Step Spherical Gold Nanoparticles
CS11-(SIZE)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
One Step Gold Nanorods
One Step Gold Nanorods
CS12-(DIAM-SPR)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

 

 

 

 

Part # Diameter (nm) Peak SPR Wavelength (nm) Optical Density (1cm) NPS/ml Wt. conc (mg/ml) Wt. % PPM Molarity (pM) Molar Ext. (M-1cm-1) Absorption Molar Ext. (M-1cm-1) Scattering Molar Ext. (M-1cm-1) Size Dispersity %PDI Notes
CS11-1.8 1.8 505-508 50 4.25E+16 2.5 0.25% 2500 7.10E+07 7.05E+05 7.05E+05 0.00E+00 <35% 1,3
CS11-2.2 2.2 505-508 50 2.33E+16 2.5 0.25% 2500 3.90E+07 1.29E+06 1.29E+06 0.00E+00 <25% 1,3
CS11-3 3 505-508 50 9.19E+15 2.5 0.25% 2500 1.50E+07 3.26E+06 3.26E+06 0.00E+00 <20% 1,3
CS11-4 4 505-508 50 3.88E+15 2.5 0.25% 2500 6.50E+06 7.74E+06 7.74E+06 0.00E+00 <20% 1,3
CS11-5 5 505-508 50 1.99E+15 2.5 0.25% 2500 3.30E+06 1.51E+07 1.51E+07 0.00E+00 <20% 2,3
CS11-10 10 514-520 50 2.48E+14 2.5 0.25% 2500 4.10E+05 1.21E+08 1.21E+08 0.00E+00 <15% 2,3
CS11-15 15 514-520 50 7.35E+13 2.5 0.25% 2500 1.20E+05 4.08E+08 4.08E+08 0.00E+00 <15% 2,3
CS11-20 20 514-520 50 3.10E+13 2.5 0.25% 2500 5.20E+04 9.67E+08 9.67E+08 0.00E+00 <10% 2,3
CS11-25 25 514-520 50 1.59E+13 2.5 0.25% 2500 2.60E+04 1.89E+09 1.89E+09 0.00E+00 <10% 2
CS11-30 30 523 50 9.19E+12 2.5 0.25% 2500 1.50E+04 3.26E+09 3.26E+09 0.00E+00 <6% 2
CS11-35 35 526 50 5.79E+12 2.5 0.25% 2500 9.60E+03 5.18E+09 5.18E+09 0.00E+00 <6% 2
CS11-40 40 527 50 3.88E+12 2.5 0.25% 2500 6.50E+03 7.74E+09 7.72E+09 2.24E+07 <4% 2
CS11-45 45 529 50 2.72E+12 2.5 0.25% 2500 4.50E+03 1.10E+10 1.08E+10 2.03E+08 <4% 2
CS11-50 50 531 50 1.99E+12 2.5 0.25% 2500 3.30E+03 1.51E+10 1.45E+10 5.88E+08 <4% 2
CS11-55 55 533 50 1.49E+12 2.5 0.25% 2500 2.50E+03 2.01E+10 1.88E+10 1.30E+09 <4% 2
CS11-60 60 536 50 1.15E+12 2.5 0.25% 2500 1.90E+03 2.61E+10 2.36E+10 2.48E+09 <4% 2
CS11-65 65 539 50 9.04E+11 2.5 0.25% 2500 1.50E+03 3.32E+10 2.89E+10 4.33E+09 <4% 2
CS11-70 70 542 50 7.23E+11 2.5 0.25% 2500 1.20E+03 4.15E+10 3.44E+10 7.09E+09 <4% 2
CS11-75 75 545 50 5.88E+11 2.5 0.25% 2500 9.80E+02 5.10E+10 4.00E+10 1.10E+10 <4% 2
CS11-80 80 549 50 4.85E+11 2.5 0.25% 2500 8.10E+02 6.19E+10 4.54E+10 1.65E+10 <4% 2
CS11-85 85 553 50 4.04E+11 2.5 0.25% 2500 6.70E+02 7.42E+10 5.03E+10 2.39E+10 <4% 2
CS11-90 90 558 50 3.40E+11 2.5 0.25% 2500 5.70E+02 8.81E+10 5.44E+10 3.37E+10 <4% 2
CS11-95 95 563 50 2.89E+11 2.5 0.25% 2500 4.80E+02 1.04E+11 5.72E+10 4.65E+10 <4% 2
CS11-100 100 569 50 2.48E+11 2.5 0.25% 2500 4.10E+02 1.21E+11 5.82E+10 6.27E+10 <4% 2
CS11-125 125 600 50 1.27E+11 2.5 0.25% 2500 2.10E+02 2.36E+11 1.53E+11 8.32E+10 <4% 2
CS11-150 150 612 50 7.35E+10 2.5 0.25% 2500 1.20E+02 4.08E+11 2.64E+11 1.44E+11 <4% 2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >=50 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) Peak LSPR Wave (nm) OD LSPR (AU) Nanorod Vol (nm3) Nanorods /mL Wt. conc (mg/ml) Wt. % PPM Molarity (nM) SPR Molar Ext. (M-1cm-1) LSPR Molar Ext. (M-1cm-1) Peak SPR accuracy (nm) SPR Linewidth 80% (nm)
CS12-10-2100 10 175 2100 17.5 5 510 1 1.35E+04 6.73E+11 0.175 0.02% 175 1.12 4.46E+09 8.92E+08 1900-2300 300
CS12-10-1400 10 102 1400 10.2 5 510 1 7.77E+03 1.17E+12 0.175 0.02% 175 1.95 2.57E+09 5.14E+08 1300-1500 150
CS12-10-1200 10 81 1200 8.1 5 510 1 6.13E+03 1.48E+12 0.175 0.02% 175 2.47 2.03E+09 4.05E+08 1132-1300 150
CS12-10-1064 10 67 1064 6.7 50 510 10 5.02E+03 1.81E+13 1.75 0.18% 1750 30.13 1.66E+09 3.32E+08 1022-1132 150
CS12-10-980 10 59 980 5.9 50 510 10 4.33E+03 2.09E+13 1.75 0.18% 1750 34.91 1.43E+09 2.86E+08 965-1022 150
CS12-10-950 10 55 950 5.5 50 510 10 4.09E+03 2.22E+13 1.75 0.18% 1750 37 1.35E+09 2.70E+08 925-965 150
CS12-10-900 10 50 900 5 50 510 10 3.68E+03 2.47E+13 1.75 0.18% 1750 41.11 1.22E+09 2.43E+08 875-925 150
CS12-10-850 10 45 850 4.5 50 510 10 3.27E+03 2.78E+13 1.75 0.18% 1750 46.25 1.08E+09 2.16E+08 829-875 100
CS12-10-808 10 41 808 4.1 50 510 10 2.93E+03 3.10E+13 1.75 0.18% 1750 51.68 9.68E+08 1.94E+08 794-829 75
CS12-10-780 10 38 780 3.8 50 510 10 2.70E+03 3.36E+13 1.75 0.18% 1750 56.06 8.92E+08 1.78E+08 765-794 65
CS12-10-750 10 35 750 3.5 50 510 10 2.45E+03 3.70E+13 1.75 0.18% 1750 61.67 8.11E+08 1.62E+08 725-765 50
CS12-10-700 10 29 700 2.9 50 510 10 2.04E+03 4.44E+13 1.75 0.18% 1750 74 6.76E+08 1.35E+08 675-725 40
CS12-10-650 10 24 650 2.4 50 510 10 1.64E+03 5.55E+13 1.75 0.18% 1750 92.5 5.41E+08 1.08E+08 625-675 40
CS12-10-600 10 19 600 1.9 50 510 10 1.23E+03 7.40E+13 1.75 0.18% 1750 123.34 4.05E+08 8.11E+07 575-625 40
CS12-25-1400 25 245 1400 9.8 5 514 1 1.16E+05 1.12E+11 0.25 0.03% 250 0.19 2.69E+10 5.37E+09 1232-1500 150
CS12-25-1064 25 137 1064 5.5 5 514 1 6.31E+04 2.05E+11 0.25 0.03% 250 0.34 1.46E+10 2.92E+09 1022-1232 150
CS12-25-980 25 119 980 4.8 50 514 10 5.43E+04 2.39E+12 2.5 0.25% 2500 3.98 1.26E+10 2.51E+09 965-1022 150
CS12-25-950 25 102 950 4.1 50 514 10 4.60E+04 2.82E+12 2.5 0.25% 2500 4.7 1.06E+10 2.13E+09 925-965 150
CS12-25-900 25 96 900 3.8 50 514 10 4.30E+04 3.01E+12 2.5 0.25% 2500 5.02 9.95E+09 1.99E+09 875-925 150
CS12-25-850 25 93 850 3.7 50 514 10 4.15E+04 3.12E+12 2.5 0.25% 2500 5.2 9.61E+09 1.92E+09 829-875 150
CS12-25-808 25 90 808 3.6 50 514 10 4.01E+04 3.24E+12 2.5 0.25% 2500 5.39 9.27E+09 1.85E+09 794-829 150
CS12-25-780 25 87 780 3.5 50 514 10 3.86E+04 3.36E+12 2.5 0.25% 2500 5.6 8.93E+09 1.79E+09 765-794 150
CS12-25-750 25 85 750 3.4 50 514 10 3.76E+04 3.45E+12 2.5 0.25% 2500 5.75 8.70E+09 1.74E+09 725-765 65
CS12-25-700 25 75 700 3 50 514 10 3.27E+04 3.96E+12 2.5 0.25% 2500 6.61 7.57E+09 1.51E+09 675-725 60
CS12-25-650 25 71 650 2.8 50 514 10 3.07E+04 4.22E+12 2.5 0.25% 2500 7.03 7.11E+09 1.42E+09 625-675 60
CS12-25-600 25 57 600 2.3 50 514 10 2.39E+04 5.43E+12 2.5 0.25% 2500 9.05 5.52E+09 1.10E+09 575-625 60
CS12-25-550 25 34 550 1.4 50 514 10 1.28E+04 1.01E+13 2.5 0.25% 2500 16.91 2.96E+09 5.91E+08 525-575 60
CS12-40-850 40 148 850 3.7 50 520 10 1.69E+05 7.67E+11 2.5 0.25% 2500 1.28 3.91E+10 7.83E+09 829-875 60
CS12-40-808 40 134 808 3.4 50 520 10 1.52E+05 8.56E+11 2.5 0.25% 2500 1.43 3.51E+10 7.01E+09 794-829 60
CS12-40-780 40 124 780 3.1 50 520 10 1.39E+05 9.33E+11 2.5 0.25% 2500 1.55 3.22E+10 6.43E+09 765-794 60
CS12-40-750 40 112 750 2.8 50 520 10 1.24E+05 1.05E+12 2.5 0.25% 2500 1.74 2.87E+10 5.73E+09 725-765 60
CS12-40-700 40 92 700 2.3 50 520 10 9.88E+04 1.31E+12 2.5 0.25% 2500 2.19 2.29E+10 4.57E+09 675-725 60
CS12-40-650 40 80 650 2 50 520 10 8.37E+04 1.55E+12 2.5 0.25% 2500 2.58 1.94E+10 3.87E+09 625-675 60
CS12-40-600 40 68 600 1.7 50 520 10 6.87E+04 1.89E+12 2.5 0.25% 2500 3.15 1.59E+10 3.18E+09 575-625 60
CS12-40-550 40 60 550 1.5 50 520 10 5.86E+04 2.21E+12 2.5 0.25% 2500 3.69 1.36E+10 2.71E+09 525-575 60
CS12-50-800 50 145 800 2.9 50 530 10 2.52E+05 5.15E+11 2.5 0.25% 2500 0.86 5.83E+10 1.17E+10 750-825 60
CS12-50-700 50 110 700 2.2 50 530 10 1.83E+05 7.08E+11 2.5 0.25% 2500 1.18 4.24E+10 8.48E+09 650-750 60
CS12-50-600 50 100 600 2 50 530 10 1.64E+05 7.93E+11 2.5 0.25% 2500 1.32 3.78E+10 7.57E+09 550-650 60

 

 

NHS conjugated gold nanoparticles

NHS functionalized gold nanoparticle

 

Composition

These bare gold nanorods are shipped highly concentrated and dried.

Quantity

This product is available in 2.5mg. For multiple kits amounting to over 100mg per annum, please contact sales for quantity pricing.

Delivery

All gold nanoparticles are in stock. This product ships in 24 hours.

Conjugations

Antibodies, peptides, any target with a primary amine.

Buffer

Customer determined. Normally the buffer that is associated with the added antibody.

Instructions

Instructions are included.

Shelf Life/Storage Temperature

This product is guaranteed for two months and should be stored in a non freezing refrigerator.

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Utilizing Nanopartz™ patent pending technologies in one step conjugations, customers now have the ability to covalently conjugate Nanopartz™ spherical gold nanoparticles and gold nanorods to most antibodies. Customers simply add the antibodies in PBS solution to the Nanopartz™ prepared gold nanoparticles, vortex for 30 minutes, and then centrifuge twice for purification. The Nanopartz™ proprietary ligand will conjugate directly to the primary amine of the target antibody, producing a visible SPR change in the gold that can be monitored by a simple UV VIS. This product is specifically for use in analytical diagnostics. This is not recommended for in vivo applications. For customers that want gold characterized by NIST traceable TEM, UV VIS, DLS, HPLC, and ICP instrumentation, we highly recommend our conjugation services.

 

 

  Nanopartz™ Gold Nanoparticles for One Step Conjugations Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized
  • Low cost
  • Works in varied pH and high salt environments
  • Customer can focus on quickly conjugating antibodies using Nanopartz™ expertise
  • Customer can inexpensively create feasibility data for experiments
  • Quick turnaround

 

How easy is your kit to use?

Simply add your antibody to the provided gold nanoparticles. If lyophilized, add 1 mL of PBS. Vortex for 30 minutes. Centrifuge twice at the recommended speeds. That simple!

But when I've used adsorbed technology in the past, salt and other additives that came with the antibodies made my gold nanoparticle turn blue and aggregate. Will that happen here?

No.

How long will the resultant product last?

That all depends on the antibody and the sterilitity of your antibody and your process. For high quality and highly characterized product, we recommend you have us perform the conjugation.

Can I choose any buffer?

Just about.

Your saying that I will see an SPR shift upon conjugation, and no shift if there is no conjugation?

Absolutely.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

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