25nm Nanorod UVVIS

Nanopartz™ Gold Nanoparticles for Zero Order Diagnostics

Nanopartz™ Gold Nanoparticles for zero order diagnostics utilize the complete line of nanospheres and nanorods for SPR shifting from 500nm to the near IR. These particles are covalently conjugated to short length linkers to optimize the SPR shift of the particle in response to a conjugation. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. This product comes in two concentrations, 2.5mg/mL and 12.5mg/mL. The highly concentrated gold nanoparticles come in a range of conjugations.

“It’s indeed a useful product that could find its way to a number of applications and customers including plasmonic bio-sensing experiments ”

Radwanul Hasan Siddique Research Group Lead at Samsung R&D USA Pasadena, California

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Conjugated Spherical Gold Nanoparticles for Zero Order Diagnostics
Conjugated Spherical Gold Nanoparticles for Zero Order Diagnostics
Part# CZ11-(SIZE)-(CONJUGATION)-(OD-mLs)
Price$480.00
Conjugated Gold Nanorods for Zero Order Diagnostics
Conjugated Gold Nanorods for Zero Order Diagnostics
Part#CZ12-(DIAM/SPR)-(CONJUGATION)-(OD-mLs)-(VOL)
DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
Price$480.00
Custom Oligo Conjugated Spherical Gold Nanoparticles for Zero Order Diagnostics
Custom Oligo Conjugated Spherical Gold Nanoparticles for Zero Order Diagnostics
Part# CZ11L-(SIZE)-(OLIGO)-(OD-mLs) Up to 30BPs
Specify either Au-polymer-3'-oligo-5' or Au-polymer-5'-oligo-3', and replace "oligo" with desired oligo sequence
Price$780.00
Custom Conjugated Spherical Gold Nanoparticles for Zero Order Diagnostics
Custom Conjugated Spherical Gold Nanoparticles for Zero Order Diagnostics
Part# CZ11R-(SIZE)-(TERM GROUP)-(OD-mLs) This product requires a quote. Please enter the following parameters and add to cart. Complete your contact information and submit for quote.
Price$0.00
Custom Oligo Conjugated Nanorods for Zero Order Diagnostics
Custom Oligo Conjugated Nanorods for Zero Order Diagnostics
Part# CZ12L-(DIAM/SPR)-(OLIGO)-(OD-mLs) Up to 30BP
Specify either Au-polymer-3'-oligo-5' or Au-polymer-5'-oligo-3', and replace "oligo" with desired oligo sequence
Price$780.00
Custom Conjugated Nanorods for Zero Order Diagnostics
Custom Conjugated Nanorods for Zero Order Diagnostics
Part# CZ12R-(DIAM/SPR)-(TERM GROUP)-(OD-mLs) This product requires a quote. Please enter the following parameters and add to cart. Complete your contact information and submit for quote.
Price$0.00

 

 

 

 

Part # Diameter (nm) Peak SPR Wavelength (nm) Optical Density (1cm) NPS/ml Wt. conc (mg/ml) Wt. % PPM Molarity (pM) Molar Ext. (M-1cm-1) AEsorption Molar Ext. (M-1cm-1) Scattering Molar Ext. (M-1cm-1) Size Dispersity %PDI Notes
CZ11-1.8 1.8 505-508 50 4.25E+16 2.5 0.25% 2500 7.10E+07 7.05E+05 7.05E+05 0.00E+00 <35% 1,3
CZ11-2.2 2.2 505-508 50 2.33E+16 2.5 0.25% 2500 3.90E+07 1.29E+06 1.29E+06 0.00E+00 <25% 1,3
CZ11-3 3 505-508 50 9.19E+15 2.5 0.25% 2500 1.50E+07 3.26E+06 3.26E+06 0.00E+00 <20% 1,3
CZ11-4 4 505-508 50 3.88E+15 2.5 0.25% 2500 6.50E+06 7.74E+06 7.74E+06 0.00E+00 <20% 1,3
CZ11-5 5 505-508 50 1.99E+15 2.5 0.25% 2500 3.30E+06 1.51E+07 1.51E+07 0.00E+00 <20% 2,3
CZ11-10 10 514-520 50 2.48E+14 2.5 0.25% 2500 4.10E+05 1.21E+08 1.21E+08 0.00E+00 <15% 2,3
CZ11-15 15 514-520 50 7.35E+13 2.5 0.25% 2500 1.20E+05 4.08E+08 4.08E+08 0.00E+00 <15% 2,3
CZ11-20 20 514-520 50 3.10E+13 2.5 0.25% 2500 5.20E+04 9.67E+08 9.67E+08 0.00E+00 <10% 2,3
CZ11-25 25 514-520 50 1.59E+13 2.5 0.25% 2500 2.60E+04 1.89E+09 1.89E+09 0.00E+00 <10% 2
CZ11-30 30 523 50 9.19E+12 2.5 0.25% 2500 1.50E+04 3.26E+09 3.26E+09 0.00E+00 <6% 2
CZ11-35 35 526 50 5.79E+12 2.5 0.25% 2500 9.60E+03 5.18E+09 5.18E+09 0.00E+00 <6% 2
CZ11-40 40 527 50 3.88E+12 2.5 0.25% 2500 6.50E+03 7.74E+09 7.72E+09 2.24E+07 <4% 2
CZ11-45 45 529 50 2.72E+12 2.5 0.25% 2500 4.50E+03 1.10E+10 1.08E+10 2.03E+08 <4% 2
CZ11-50 50 531 50 1.99E+12 2.5 0.25% 2500 3.30E+03 1.51E+10 1.45E+10 5.88E+08 <4% 2
CZ11-55 55 533 50 1.49E+12 2.5 0.25% 2500 2.50E+03 2.01E+10 1.88E+10 1.30E+09 <4% 2
CZ11-60 60 536 50 1.15E+12 2.5 0.25% 2500 1.90E+03 2.61E+10 2.36E+10 2.48E+09 <4% 2
CZ11-65 65 539 50 9.04E+11 2.5 0.25% 2500 1.50E+03 3.32E+10 2.89E+10 4.33E+09 <4% 2
CZ11-70 70 542 50 7.23E+11 2.5 0.25% 2500 1.20E+03 4.15E+10 3.44E+10 7.09E+09 <4% 2
CZ11-75 75 545 50 5.88E+11 2.5 0.25% 2500 9.80E+02 5.10E+10 4.00E+10 1.10E+10 <4% 2
CZ11-80 80 549 50 4.85E+11 2.5 0.25% 2500 8.10E+02 6.19E+10 4.54E+10 1.65E+10 <4% 2
CZ11-85 85 553 50 4.04E+11 2.5 0.25% 2500 6.70E+02 7.42E+10 5.03E+10 2.39E+10 <4% 2
CZ11-90 90 558 50 3.40E+11 2.5 0.25% 2500 5.70E+02 8.81E+10 5.44E+10 3.37E+10 <4% 2
CZ11-95 95 563 50 2.89E+11 2.5 0.25% 2500 4.80E+02 1.04E+11 5.72E+10 4.65E+10 <4% 2
CZ11-100 100 569 50 2.48E+11 2.5 0.25% 2500 4.10E+02 1.21E+11 5.82E+10 6.27E+10 <4% 2
CZ11-125 125 600 50 1.27E+11 2.5 0.25% 2500 2.10E+02 2.36E+11 1.53E+11 8.32E+10 <4% 2
CZ11-150 150 612 50 7.35E+10 2.5 0.25% 2500 1.20E+02 4.08E+11 2.64E+11 1.44E+11 <4% 2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >=50 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) Peak LSPR Wave (nm) OD LSPR (AU) Nanorod Vol (nm3) Nanorods /mL Wt. conc (mg/ml) Wt. % PPM Molarity (nM) SPR Molar Ext. (M-1cm-1) LSPR Molar Ext. (M-1cm-1) Peak SPR accuracy (nm) SPR Linewidth 80% (nm)
CZ12-10-2100 10 175 2100 17.5 5 510 1 1.35E+04 6.73E+11 0.175 0.02% 175 1.12 4.46E+09 8.92E+08 1900-2300 300
CZ12-10-1400 10 102 1400 10.2 5 510 1 7.77E+03 1.17E+12 0.175 0.02% 175 1.95 2.57E+09 5.14E+08 1300-1500 150
CZ12-10-1200 10 81 1200 8.1 5 510 1 6.13E+03 1.48E+12 0.175 0.02% 175 2.47 2.03E+09 4.05E+08 1132-1300 150
CZ12-10-1064 10 67 1064 6.7 50 510 10 5.02E+03 1.81E+13 1.75 0.18% 1750 30.13 1.66E+09 3.32E+08 1022-1132 150
CZ12-10-980 10 59 980 5.9 50 510 10 4.33E+03 2.09E+13 1.75 0.18% 1750 34.91 1.43E+09 2.86E+08 965-1022 150
CZ12-10-950 10 55 950 5.5 50 510 10 4.09E+03 2.22E+13 1.75 0.18% 1750 37 1.35E+09 2.70E+08 925-965 150
CZ12-10-900 10 50 900 5 50 510 10 3.68E+03 2.47E+13 1.75 0.18% 1750 41.11 1.22E+09 2.43E+08 875-925 150
CZ12-10-850 10 45 850 4.5 50 510 10 3.27E+03 2.78E+13 1.75 0.18% 1750 46.25 1.08E+09 2.16E+08 829-875 100
CZ12-10-808 10 41 808 4.1 50 510 10 2.93E+03 3.10E+13 1.75 0.18% 1750 51.68 9.68E+08 1.94E+08 794-829 75
CZ12-10-780 10 38 780 3.8 50 510 10 2.70E+03 3.36E+13 1.75 0.18% 1750 56.06 8.92E+08 1.78E+08 765-794 65
CZ12-10-750 10 35 750 3.5 50 510 10 2.45E+03 3.70E+13 1.75 0.18% 1750 61.67 8.11E+08 1.62E+08 725-765 50
CZ12-10-700 10 29 700 2.9 50 510 10 2.04E+03 4.44E+13 1.75 0.18% 1750 74 6.76E+08 1.35E+08 675-725 40
CZ12-10-650 10 24 650 2.4 50 510 10 1.64E+03 5.55E+13 1.75 0.18% 1750 92.5 5.41E+08 1.08E+08 625-675 40
CZ12-10-600 10 19 600 1.9 50 510 10 1.23E+03 7.40E+13 1.75 0.18% 1750 123.34 4.05E+08 8.11E+07 575-625 40
CZ12-25-1400 25 245 1400 9.8 5 514 1 1.16E+05 1.12E+11 0.25 0.03% 250 0.19 2.69E+10 5.37E+09 1232-1500 150
CZ12-25-1064 25 137 1064 5.5 5 514 1 6.31E+04 2.05E+11 0.25 0.03% 250 0.34 1.46E+10 2.92E+09 1022-1232 150
CZ12-25-980 25 119 980 4.8 50 514 10 5.43E+04 2.39E+12 2.5 0.25% 2500 3.98 1.26E+10 2.51E+09 965-1022 150
CZ12-25-950 25 102 950 4.1 50 514 10 4.60E+04 2.82E+12 2.5 0.25% 2500 4.7 1.06E+10 2.13E+09 925-965 150
CZ12-25-900 25 96 900 3.8 50 514 10 4.30E+04 3.01E+12 2.5 0.25% 2500 5.02 9.95E+09 1.99E+09 875-925 150
CZ12-25-850 25 93 850 3.7 50 514 10 4.15E+04 3.12E+12 2.5 0.25% 2500 5.2 9.61E+09 1.92E+09 829-875 150
CZ12-25-808 25 90 808 3.6 50 514 10 4.01E+04 3.24E+12 2.5 0.25% 2500 5.39 9.27E+09 1.85E+09 794-829 150
CZ12-25-780 25 87 780 3.5 50 514 10 3.86E+04 3.36E+12 2.5 0.25% 2500 5.6 8.93E+09 1.79E+09 765-794 150
CZ12-25-750 25 85 750 3.4 50 514 10 3.76E+04 3.45E+12 2.5 0.25% 2500 5.75 8.70E+09 1.74E+09 725-765 65
CZ12-25-700 25 75 700 3 50 514 10 3.27E+04 3.96E+12 2.5 0.25% 2500 6.61 7.57E+09 1.51E+09 675-725 60
CZ12-25-650 25 71 650 2.8 50 514 10 3.07E+04 4.22E+12 2.5 0.25% 2500 7.03 7.11E+09 1.42E+09 625-675 60
CZ12-25-600 25 57 600 2.3 50 514 10 2.39E+04 5.43E+12 2.5 0.25% 2500 9.05 5.52E+09 1.10E+09 575-625 60
CZ12-25-550 25 34 550 1.4 50 514 10 1.28E+04 1.01E+13 2.5 0.25% 2500 16.91 2.96E+09 5.91E+08 525-575 60
CZ12-40-850 40 148 850 3.7 50 520 10 1.69E+05 7.67E+11 2.5 0.25% 2500 1.28 3.91E+10 7.83E+09 829-875 60
CZ12-40-808 40 134 808 3.4 50 520 10 1.52E+05 8.56E+11 2.5 0.25% 2500 1.43 3.51E+10 7.01E+09 794-829 60
CZ12-40-780 40 124 780 3.1 50 520 10 1.39E+05 9.33E+11 2.5 0.25% 2500 1.55 3.22E+10 6.43E+09 765-794 60
CZ12-40-750 40 112 750 2.8 50 520 10 1.24E+05 1.05E+12 2.5 0.25% 2500 1.74 2.87E+10 5.73E+09 725-765 60
CZ12-40-700 40 92 700 2.3 50 520 10 9.88E+04 1.31E+12 2.5 0.25% 2500 2.19 2.29E+10 4.57E+09 675-725 60
CZ12-40-650 40 80 650 2 50 520 10 8.37E+04 1.55E+12 2.5 0.25% 2500 2.58 1.94E+10 3.87E+09 625-675 60
CZ12-40-600 40 68 600 1.7 50 520 10 6.87E+04 1.89E+12 2.5 0.25% 2500 3.15 1.59E+10 3.18E+09 575-625 60
CZ12-40-550 40 60 550 1.5 50 520 10 5.86E+04 2.21E+12 2.5 0.25% 2500 3.69 1.36E+10 2.71E+09 525-575 60
CZ12-50-800 50 145 800 2.9 50 530 10 2.52E+05 5.15E+11 2.5 0.25% 2500 0.86 5.83E+10 1.17E+10 750-825 60
CZ12-50-700 50 110 700 2.2 50 530 10 1.83E+05 7.08E+11 2.5 0.25% 2500 1.18 4.24E+10 8.48E+09 650-750 60
CZ12-50-600 50 100 600 2 50 530 10 1.64E+05 7.93E+11 2.5 0.25% 2500 1.32 3.78E+10 7.57E+09 550-650 60

 

 

 

 

zero order construct

Nanopartz™ covalently bonded Zero Order Construct.

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
Secondary antibodies  

 

 

Composition

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

  1. Amine
  2. Biotin
  3. Carboxyl
  4. Neutravidin
  5. Streptavidin
  6. Azide

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

These gold nanoparticles exhibit extinctions from 500nm to 1064nm, perfect for use in UV-VIS analytical systems. These gold nanoparticles come with a very short length covalent conjugation, specifically suited for zero order diagnostics as it is a well known property that the extinction of gold nanoparticles is very sensitive to the local index of refraction. The covalent attachment ensures minimal non specific binding. The customer has a choice for radiation sterilization (no sodium azide is used), certification and endotoxin free products.

 

 

  Nanopartz™ Gold Nanoparticles for Zero Order Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

  • Extremely stable. Shelf life is greater than six months.
  • Covalent bonds.
  • High anisotropy.
  • No cytotoxicity.
  • Nanorod to sphere shape changing on high energy pulsed lasers.
  • Bright Fluorescence - intense emission provides superior sensitivity and requires less conjugate.
  • Narrow Emission spectra - covalent bonds ensure no bleed through.
  • Excellent photostability - exceptional resistance to photobleaching enables fluorescence imaging uner the most demanding conditions.
  • Buffer Stability - stable from pH 4-9.
  • Instrument cability - excitation and emission spectra correspond with filter sets and laser setting of all popular fluorescence instrumentation.
  • Customer can focus on research and not on Nanopartz expertise
  • Quick turnaround

 

Spherical Gold UV VIS Gold Nanorods UV VIS

 

SDS

How sensitive is the product?

That depends on a number of factors including the size and shape of the gold nanoparticle and the size of the target protein. In general, nanorods are more sensitive than spheres (shifts in aspect ratio result in a greater SPR change than a change in diameter for spheres), and larger particles are more sensitive than smaller ones. Also, a system targeting larger proteins will be more sensitive than one targeting smaller proteins.

Another very important variable is whether the system is a homogeneous or heterogeneous system. In general, a homogeneous system will measure many particles simultaneously (normally through a path length). A heterogenous system can be designed to look at individual, fixed gold nanoparticles on a slide (for example). Consequently, the fact that single particle statistics are more sensitive than bulk particle statistics, a heterogeneous system will be more sensitive.

Given these parameters, publications have shown attomolar detection for nanorods using a biotin/streptavidin system in a heterogenous system on a glass slide. A good example of this is shown in Nucz et al. "Label-Free Plasmonic Detection of Biomolecular Binding by a Single Gold Nanorod" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003601/ .

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

Enhancing sensitivity of surface plasmon resonance biosensors by functionalized gold nanoparticles: size matters

T Špringer, ML Ermini, B Špačková… - Analytical …, 2014 - ACS Publications
... The extinction coefficients (obtained from Nanopartz Inc.) for the 10, 15, 21, 33, and 52 nm
N-AuNPs were 9.5 × 10 7 , 3.2 × 10 8 , 8.8 × 10 8 , 3.3 × 10 ... This response is sensitive to changes
in the refractive index caused by the binding of molecules to the surface of an SPR chip ...

 

Size dependence of Au NP-enhanced surface plasmon resonance based on differential phase measurement

S Zeng, X Yu, WC Law, Y Zhang, R Hu, XQ Dinh… - Sensors and Actuators B …, 2013 - Elsevier
... nm, 60 nm, 70 nm and 80 nm were purchased from Nanopartz Accurate ... open a new pathway
for the development of ultra-sensitive SPR sensors based ... Sensitivity improved surface plasmon
resonance biosensor for cancer biomarker detection based on plasmonic enhancement. ...

 

Nanomaterials enhanced surface plasmon resonance for biological and chemical sensing applications

S Zeng, D Baillargeat, HP Ho, KT Yong - Chemical Society Reviews, 2014 - pubs.rsc.org
... the summary and future outlook for engineering the nanomaterials to further improve plasmonic
sensing of ... In this study, the SPR sensitivity for the detection of 24-mer ssDNA oligonucleotides ...
coupling of LSPR excited on the Au NPs with the hybrid surface plasmon waves that ...

 

Portable mercury sensor for tap water using surface plasmon resonance of immobilized gold nanorods

EC Heider, K Trieu, AFT Moore, AD Campiglia - Talanta, 2012 - Elsevier
... 10 11 nanorods/mL), and 750 nm (5.77×10 10 nanorods/mL) were purchased from Nanopartz,
Inc ... the number of particles present on the surface of each sensing device, the SPR response
(Δλ ... agreement with the SEM images that show most Au NR flattened on the surface of the ...

 

Shape-dependent oriented trapping and scaffolding of plasmonic nanoparticles by topological defects for self-assembly of colloidal dimers in liquid crystals

B Senyuk, JS Evans, PJ Ackerman, T Lee, P Manna… - Nano …, 2012 - ACS Publications
... 15-18) is limited by heating,(17) the relationship between trapping and SPR wavelengths,(18 ...
characterized by the axial diameter D np and length L np ), and surface chemistry (Table ... with
NSol(akyl acrylate) polymer conjugation (Figure 1a–d) were obtained from Nanopartz, Inc. ...

 

Plasmonic nanobubbles as transient vapor nanobubbles generated around plasmonic nanoparticles

E Lukianova-Hleb, Y Hu, L Latterini, L Tarpani, S Lee… - ACS …, 2010 - ACS Publications
... Generation of Plasmonic Nanobubbles have been studied around individual gold NPs in water
by ... laser pulses that caused water evaporation around the laser-heated NP surface and the ... of
NPs regardless of their aggregation state or the position of the plasmon resonance peak ...

 

Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages

MM Janát-Amsbury, A Ray, CM Peterson… - European Journal of …, 2011 - Elsevier
... of 50 nm in diameter and rod-shaped particles with reported dimensions of 10 × 45 nm) were
purchased from Nanopartz, Inc. ... Each batch of particles was characterized by UV–VIS
spectrophotometry to determine their surface plasmon resonance (SPR) and optical ...

 

Gold nanorods for surface plasmon resonance detection of mercury (II) in flow injection analysis

K Trieu, EC Heider, SC Brooks, F Barbosa… - Talanta, 2014 - Elsevier
... of 611 nm and capped with cetyl trimethylammonium bromide (CTAB) were purchased from
Nanopartz, Inc. ... deposition on the immobilized NRs removes CTAB molecules from the surface
of the ... Shift of the SPR maximum wavelength of Au NRs substrates (Δλmax) recorded as a ...