Nanopartz Antibody Functionalized Gold Nanoparticles

Nanopartz Gold Nanoparticles, conjugated specifically for in vitro applications, utilize the complete line of Accurate Spherical Gold Nanoparticles and Gold Nanorods functionalized via a number of different methods including absorption, short carbon covalent bridges, PEG, and our own proprietary invitro covalent polymer bridge - all to the most popular primary and secondary antibodies. Using our invitro covalent polymer bridge, this product is well known for its resistantance to temperature, salt, and pH, and for its exceptional loading capability. Using our in vivo covalent polymer bridge, this product is know for its long circulation times and it exceptional loading capacity. These conjugated particles can be used in a multitude of applications, including diagnostics, imaging, particularly in adverse environments. These particles are guaranteed not to aggregate. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. Typical volume is 1mL at a concentration of 50 OD. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the antibody is measured.

image_0

 

Part #
Diameter (nm)
Peak SPR Wavelength (nm)
NPS/ml
Molarity (pM)
Moles
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Size Accuracy (+/- nm)
CA11-1.8
1.8
n/a
4.25E+16
7.10E+07
7.09E-08
7.05E+05
7.05E+05
0.00E+00
<35%
0.1
CA11-2.2
2.2
n/a
2.33E+16
3.90E+07
3.88E-08
1.29E+06
1.29E+06
0.00E+00
<25%
0.1
CA11-3
3
n/a
9.19E+15
1.50E+07
1.53E-08
3.26E+06
3.26E+06
0.00E+00
<20%
0.1
CA11-4
4
n/a
3.88E+15
6.50E+06
6.46E-09
7.74E+06
7.74E+06
0.00E+00
<20%
1
CA11-5
5
512
1.99E+15
3.30E+06
3.31E-09
1.51E+07
1.51E+07
0.00E+00
<20%
2
CA11-10
10
516
2.48E+14
4.10E+05
4.14E-10
1.21E+08
1.21E+08
0.00E+00
<15%
2
CA11-15
15
518
7.35E+13
1.20E+05
1.23E-10
4.08E+08
4.08E+08
0.00E+00
<15%
2
CA11-20
20
520
3.10E+13
5.20E+04
5.17E-11
9.67E+08
9.67E+08
0.00E+00
<10%
2
CA11-25
25
521
1.59E+13
2.60E+04
2.65E-11
1.89E+09
1.89E+09
0.00E+00
<10%
2
CA11-30
30
523
9.19E+12
1.50E+04
1.53E-11
3.26E+09
3.26E+09
0.00E+00
<6%
2
CA11-35
35
526
5.79E+12
9.60E+03
9.65E-12
5.18E+09
5.18E+09
0.00E+00
<6%
2
CA11-40
40
527
3.88E+12
6.50E+03
6.46E-12
7.74E+09
7.72E+09
2.24E+07
<4%
2
CA11-45
45
529
2.72E+12
4.50E+03
4.54E-12
1.10E+10
1.08E+10
2.03E+08
<4%
2
CA11-50
50
531
1.99E+12
3.30E+03
3.31E-12
1.51E+10
1.45E+10
5.88E+08
<4%
2
CA11-55
55
533
1.49E+12
2.50E+03
2.49E-12
2.01E+10
1.88E+10
1.30E+09
<4%
2
CA11-60
60
536
1.15E+12
1.90E+03
1.91E-12
2.61E+10
2.36E+10
2.48E+09
<4%
2
CA11-65
65
539
9.04E+11
1.50E+03
1.51E-12
3.32E+10
2.89E+10
4.33E+09
<4%
2
CA11-70
70
542
7.23E+11
1.20E+03
1.21E-12
4.15E+10
3.44E+10
7.09E+09
<4%
2
CA11-75
75
545
5.88E+11
9.80E+02
9.80E-13
5.10E+10
4.00E+10
1.10E+10
<4%
2
CA11-80
80
549
4.85E+11
8.10E+02
8.08E-13
6.19E+10
4.54E+10
1.65E+10
<4%
2
CA11-85
85
553
4.04E+11
6.70E+02
6.73E-13
7.42E+10
5.03E+10
2.39E+10
<4%
2
CA11-90
90
558
3.40E+11
5.70E+02
5.67E-13
8.81E+10
5.44E+10
3.37E+10
<4%
2
CA11-95
95
563
2.89E+11
4.80E+02
4.82E-13
1.04E+11
5.72E+10
4.65E+10
<4%
2
CA11-100
100
569
2.48E+11
4.10E+02
4.14E-13
1.21E+11
5.82E+10
6.27E+10
<4%
2
CA11-150
150
612
7.35E+10
1.20E+02
1.23E-13
4.08E+11
0.00E+00
4.08E+11
<4%
10
CA11-200
200
n/a
3.10E+10
5.20E+01
5.17E-14
9.67E+11
0.00E+00
9.67E+11
<4%
10
CA11-500
500
n/a
1.99E+09
3.30E+00
3.31E-15
1.51E+13
0.00E+00
1.51E+13
<4%
50
CA11-1000
1000
n/a
2.48E+08
4.10E-01
4.14E-16
1.21E+14
0.00E+00
1.21E+14
<4%
100
CA11-1500
1500
n/a
7.35E+07
1.20E-01
1.23E-16
4.08E+14
0.00E+00
4.08E+14
<4%
100

 

Optical Density >= 50, 5 for sizes >=500nm
Wt conc = 2.5mg/mL, 1mL volume typical
Wt % = 0.25%
ppm = 2500
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
Shape monodispersity (% spheres) > 99.9%
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Part #
Diameter (nm)
Length (nm)
Peak SPR Wave (nm)
Aspect Ratio
OD SPR (AU)
Peak LSPR Wave (nm)
OD LSPR (AU)
Nanorods /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (nM)
Picomoles
SPR Molar Ext. (M-1cm-1)
LSPR Molar Ext. (M-1cm-1)

Peak SPR

(nm)

SPR Linewidth 80% (nm)
CA12-5-808
5
21
808
4.2
50
510
10
2.39E+14
1750
0.18%
1750
398.72
3.99E+02
1.25E+08
2.51E+07
794-829
100
CA12-5-780
5
19
780
3.8
50
510
10
2.67E+14
1750
0.18%
1750
444.72
4.45E+02
1.12E+08
2.25E+07
765-794
100
CA12-5-700
5
15
700
3
50
510
10
3.47E+14
1750
0.18%
1750
578.14
5.78E+02
8.65E+07
1.73E+07
675-725
100
CA12-10-2100
10
175
2100
17.5
50
510
10
6.73E+12
1750
0.18%
1750
11.21
1.12E+01
4.46E+09
8.92E+08
1900-2300
300
CA12-10-1400
10
102
1400
10.2
50
510
10
1.17E+13
1750
0.18%
1750
19.47
1.95E+01
2.57E+09
5.14E+08
1300-1500
150
CA12-10-1200
10
81
1200
8.1
50
510
10
1.48E+13
1750
0.18%
1750
24.67
2.47E+01
2.03E+09
4.05E+08
1132-1300
150
CA12-10-1064
10
67
1064
6.7
50
510
10
1.81E+13
1750
0.18%
1750
30.13
3.01E+01
1.66E+09
3.32E+08
1022-1132
150
CA12-10-980
10
59
980
5.9
50
510
10
2.09E+13
1750
0.18%
1750
34.91
3.49E+01
1.43E+09
2.86E+08
965-1022
150
CA12-10-950
10
55
950
5.5
50
510
10
2.22E+13
1750
0.18%
1750
37
3.70E+01
1.35E+09
2.70E+08
925-965
150
CA12-10-900
10
50
900
5
50
510
10
2.47E+13
1750
0.18%
1750
41.11
4.11E+01
1.22E+09
2.43E+08
875-925
150
CA12-10-850
10
45
850
4.5
50
510
10
2.78E+13
1750
0.18%
1750
46.25
4.63E+01
1.08E+09
2.16E+08
829-875
100
CA12-10-808
10
41
808
4.1
50
510
10
3.10E+13
1750
0.18%
1750
51.68
5.17E+01
9.68E+08
1.94E+08
794-829
75
CA12-10-780
10
38
780
3.8
50
510
10
3.36E+13
1750
0.18%
1750
56.06
5.61E+01
8.92E+08
1.78E+08
765-794
65
CA12-10-750
10
35
750
3.5
50
510
10
3.70E+13
1750
0.18%
1750
61.67
6.17E+01
8.11E+08
1.62E+08
725-765
50
CA12-10-700
10
29
700
2.9
50
510
10
4.44E+13
1750
0.18%
1750
74
7.40E+01
6.76E+08
1.35E+08
675-725
40
CA12-10-650
10
24
650
2.4
50
510
10
5.55E+13
1750
0.18%
1750
92.5
9.25E+01
5.41E+08
1.08E+08
625-675
40
CA12-10-600
10
19
600
1.9
50
510
10
7.40E+13
1750
0.18%
1750
123.34
1.23E+02
4.05E+08
8.11E+07
575-625
40
CA12-25-1400
25
245
1400
9.8
50
514
15
1.12E+12
2500
0.25%
2500
1.86
1.86E+00
2.69E+10
8.06E+09
1232-1500
150
CA12-25-1064
25
137
1064
5.5
50
514
15
2.05E+12
2500
0.25%
2500
3.42
3.42E+00
1.46E+10
4.38E+09
1022-1232
150
CA12-25-980
25
119
980
4.8
50
514
15
2.39E+12
2500
0.25%
2500
3.98
3.98E+00
1.26E+10
3.77E+09
965-1022
150
CA12-25-950
25
102
950
4.1
50
514
15
2.82E+12
2500
0.25%
2500
4.7
4.70E+00
1.06E+10
3.19E+09
925-965
150
CA12-25-900
25
96
900
3.8
50
514
15
3.01E+12
2500
0.25%
2500
5.02
5.02E+00
9.95E+09
2.99E+09
875-925
150
CA12-25-850
25
93
850
3.7
50
514
15
3.12E+12
2500
0.25%
2500
5.2
5.20E+00
9.61E+09
2.88E+09
829-875
150
CA12-25-808
25
90
808
3.6
50
514
15
3.24E+12
2500
0.25%
2500
5.39
5.39E+00
9.27E+09
2.78E+09
794-829
150
CA12-25-780
25
87
780
3.5
50
514
15
3.36E+12
2500
0.25%
2500
5.6
5.60E+00
8.93E+09
2.68E+09
765-794
150
CA12-25-750
25
85
750
3.4
50
514
15
3.45E+12
2500
0.25%
2500
5.75
5.75E+00
8.70E+09
2.61E+09
725-765
65
CA12-25-700
25
75
700
3
50
514
15
3.96E+12
2500
0.25%
2500
6.61
6.61E+00
7.57E+09
2.27E+09
675-725
60
CA12-25-650
25
71
650
2.8
50
514
15
4.22E+12
2500
0.25%
2500
7.03
7.03E+00
7.11E+09
2.13E+09
625-675
60
CA12-25-600
25
57
600
2.3
50
514
15
5.43E+12
2500
0.25%
2500
9.05
9.05E+00
5.52E+09
1.66E+09
575-625
60
CA12-25-550
25
34
550
1.4
50
514
15
1.01E+13
2500
0.25%
2500
16.91
1.69E+01
2.96E+09
8.87E+08
525-575
60
CA12-40-1064
40
208
850
5.2
50
520
20
5.30E+11
2500
0.25%
2500
0.88
8.84E-01
5.66E+10
2.26E+10
1022-1232
150
CA12-40-980
40
180
850
4.5
50
520
20
6.19E+11
2500
0.25%
2500
1.03
1.03E+00
4.84E+10
1.94E+10
875-1022
150
CA12-40-850
40
148
850
3.7
50
520
20
7.67E+11
2500
0.25%
2500
1.28
1.28E+00
3.91E+10
1.57E+10
829-875
150
CA12-40-808
40
134
808
3.4
50
520
20
8.56E+11
2500
0.25%
2500
1.43
1.43E+00
3.51E+10
1.40E+10
794-829
150
CA12-40-780
40
124
780
3.1
50
520
20
9.33E+11
2500
0.25%
2500
1.55
1.55E+00
3.22E+10
1.29E+10
765-794
150
CA12-40-750
40
112
750
2.8
50
520
20
1.05E+12
2500
0.25%
2500
1.74
1.74E+00
2.87E+10
1.15E+10
725-765
150
CA12-40-700
40
92
700
2.3
50
520
20
1.31E+12
2500
0.25%
2500
2.19
2.19E+00
2.29E+10
9.14E+09
675-725
60
CA12-40-650
40
80
650
2
50
520
20
1.55E+12
2500
0.25%
2500
2.58
2.58E+00
1.94E+10
7.75E+09
625-675
60
CA12-40-600
40
68
600
1.7
50
520
20
1.89E+12
2500
0.25%
2500
3.15
3.15E+00
1.59E+10
6.35E+09
575-625
60
CA12-40-550
40
60
550
1.5
50
520
20
2.21E+12
2500
0.25%
2500
3.69
3.69E+00
1.36E+10
5.42E+09
525-575
60
CA12-50-1064
50
245
1064
4.9
50
530
25
2.89E+11
2500
0.25%
2500
0.48
4.82E-01
1.04E+11
5.18E+10
980-1232
150
CA12-50-808
50
145
808
2.9
50
530
25
5.15E+11
2500
0.25%
2500
0.86
8.58E-01
5.83E+10
2.91E+10
750-850
150
CA12-50-700
50
110
700
2.2
50
530
25
7.08E+11
2500
0.25%
2500
1.18
1.18E+00
4.24E+10
2.12E+10
650-750
150
CA12-50-600
50
100
600
2
50
530
25
7.93E+11
2500
0.25%
2500
1.32
1.32E+00
3.78E+10
1.89E+10
550-650
150
CA12-70-750
70
120
750
1.7
50
530
25
3.49E+11
2500
0.25%
2500
0.58
5.81E-01
8.60E+10
4.30E+10
700-800
150
CA12-70-650
70
105
650
1.5
50
530
25
4.13E+11
2500
0.25%
2500
0.69
6.88E-01
7.27E+10
3.63E+10
600-700
150
CA12-70-550
70
91
550
1.3
50
530
25
4.98E+11
2500
0.25%
2500
0.83
8.30E-01
6.02E+10
3.01E+10
500-600
150

 

Optical Density >= 50, volume = 1mL
All specs typical. May vary batch to batch. Exact values are measured for each batch
Solution default is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

Part #
Diameter (nm)
Length (nm)
Aspect Ratio
Peak SPR Wave (nm)
SPR OD (1cm)
Microgold /mL
moles
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
SPR Molar Ext. (M-1cm-1)
CA13-500
75
500
6.7
510
0.25
6.18E+09
1.03E-14
250
0.03%
250
10.3
2.43E+07
CA13-1000
100
1000
10
510
0.25
1.71E+09
2.85E-15
250
0.03%
250
2.85
8.78E+07

 

SPR = Transverse SPR peak
Longitudinal has no SPR peak
Shape monodispersity (% rods) > 95%
Size variation +/-10% (both dimensions)

 

 

Part #
Diameter (nm)
Length (nm)
Aspect Ratio
Peak SPR Wave (nm)
Nanowires Vol (nm3)
Nanowires /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
SPR Molar Ext. (M-1cm-1)
CA14-1000
75
1000
13
510
4.31E+06
3.01E+09
250
0.03%
250
5.02
9.96E+06
CA14-2000
75
2000
27
510
8.72E+06
1.49E+09
250
0.03%
250
2.48
2.02E+07
CA14-4000
75
4000
53
510
1.76E+07
7.39E+08
250
0.03%
250
1.23
4.06E+07
CA14-6000
75
6000
80
510
2.64E+07
4.91E+08
250
0.03%
250
0.82
6.10E+07
CA14-10000
75
10000
133
510
4.40E+07
2.94E+08
250
0.03%
250
0.49
1.02E+08
CA14-20000
100
20000
200
510
1.57E+08
8.27E+07
250
0.03%
250
0.14
3.63E+08
CA14-40000
140
40000
286
510
6.15E+08
2.11E+07
250
0.03%
250
0.04
1.42E+09

 

SPR = Transverse SPR peak
Longitudinal has no SPR peak
Shape monodispersity (% rods) > 95%
Size variation +/-10% (both dimensions)

 

Functionalized Nanorod Block Invitro

 

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer

 

The following tables show the typical loading for an IgG on our gold nanoparticles using PEG as the covalent polymer bridge. Our in vitro Polymer is 2x more. Our in vivo polymer is about 50% more.

 

Sphere AbLoading

Part # Diameter (nm) Peak SPR (nm) NPS/ml Molarity (pM) Moles IgG Ab/np Total (ug) IgG
CA11-1.8 1.8 n/a 4.25E+16 7.10E+07 7.09E-08 0.1 1,379
CA11-2.2 2.2 n/a 2.33E+16 3.90E+07 3.88E-08 0.2 1,128
CA11-3 3 n/a 9.19E+15 1.50E+07 1.53E-08 0.4 827
CA11-4 4 n/a 3.88E+15 6.50E+06 6.46E-09 0.6 620
CA11-5 5 512 1.99E+15 3.30E+06 3.31E-09 1 496
CA11-10 10 516 2.48E+14 4.10E+05 4.14E-10 4 248
CA11-15 15 518 7.35E+13 1.20E+05 1.23E-10 9 165
CA11-20 20 520 3.10E+13 5.20E+04 5.17E-11 16 124
CA11-25 25 521 1.59E+13 2.60E+04 2.65E-11 25 99
CA11-30 30 523 9.19E+12 1.50E+04 1.53E-11 36 83
CA11-35 35 526 5.79E+12 9.60E+03 9.65E-12 49 71
CA11-40 40 527 3.88E+12 6.50E+03 6.46E-12 64 62
CA11-45 45 529 2.72E+12 4.50E+03 4.54E-12 81 55
CA11-50 50 531 1.99E+12 3.30E+03 3.31E-12 100 50
CA11-55 55 533 1.49E+12 2.50E+03 2.49E-12 121 45
CA11-60 60 536 1.15E+12 1.90E+03 1.91E-12 144 41
CA11-65 65 539 9.04E+11 1.50E+03 1.51E-12 169 38
CA11-70 70 542 7.23E+11 1.20E+03 1.21E-12 196 35
CA11-75 75 545 5.88E+11 9.80E+02 9.80E-13 225 33
CA11-80 80 549 4.85E+11 8.10E+02 8.08E-13 256 31
CA11-85 85 553 4.04E+11 6.70E+02 6.73E-13 289 29
CA11-90 90 558 3.40E+11 5.70E+02 5.67E-13 324 28
CA11-95 95 563 2.89E+11 4.80E+02 4.82E-13 361 26
CA11-100 100 569 2.48E+11 4.10E+02 4.14E-13 400 25
CA11-150 150 612 7.35E+10 1.20E+02 1.23E-13 900 17
CA11-200 200 n/a 3.10E+10 5.20E+01 5.17E-14 1600 12
CA11-500 500 n/a 1.99E+09 3.30E+00 3.31E-15 10000 5
CA11-1000 1000 n/a 2.48E+08 4.10E-01 4.14E-16 40000 2
CA11-1500 1500 n/a 7.35E+07 1.20E-01 1.23E-16 90000 2

 

Nanorod Ab Loading

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) IgG Ab/nanorod Total (ug) IgG
CA12-5-808 5 21 808 4.2 50 4 251
CA12-5-780 5 19 780 3.8 50 4 253
CA12-5-700 5 15 700 3 50 3 260
               
CA12-10-2100 10 175 2100 17.5 50 70 118
CA12-10-1400 10 102 1400 10.2 50 41 120
CA12-10-1200 10 81 1200 8.1 50 33 121
CA12-10-1064 10 67 1064 6.7 50 27 122
CA12-10-980 10 59 980 5.9 50 23 123
CA12-10-950 10 55 950 5.5 50 22 123
CA12-10-900 10 50 900 5 50 20 124
CA12-10-850 10 45 850 4.5 50 18 125
CA12-10-808 10 41 808 4.1 50 16 126
CA12-10-780 10 38 780 3.8 50 15 127
CA12-10-750 10 35 750 3.5 50 14 128
CA12-10-700 10 29 700 2.9 50 12 130
CA12-10-650 10 24 650 2.4 50 10 134
CA12-10-600 10 19 600 1.9 50 8 140
               
CA12-25-1400 25 245 1400 9.8 50 245 68
CA12-25-1064 25 137 1064 5.5 50 137 70
CA12-25-980 25 119 980 4.8 50 119 71
CA12-25-950 25 102 950 4.1 50 102 72
CA12-25-900 25 96 900 3.8 50 96 72
CA12-25-850 25 93 850 3.7 50 93 73
CA12-25-808 25 90 808 3.6 50 90 73
CA12-25-780 25 87 780 3.5 50 87 73
CA12-25-750 25 85 750 3.4 50 85 73
CA12-25-700 25 75 700 3 50 75 74
CA12-25-650 25 71 650 2.8 50 71 75
CA12-25-600 25 57 600 2.3 50 57 77
CA12-25-550 25 34 550 1.4 50 34 87
               
CA12-40-1064 40 208 850 5.2 50 333 44
CA12-40-980 40 180 850 4.5 50 288 45
CA12-40-850 40 148 850 3.7 50 237 45
CA12-40-808 40 134 808 3.4 50 214 46
CA12-40-780 40 124 780 3.1 50 198 46
CA12-40-750 40 112 750 2.8 50 179 47
CA12-40-700 40 92 700 2.3 50 147 48
CA12-40-650 40 80 650 2 50 128 50
CA12-40-600 40 68 600 1.7 50 109 51
CA12-40-550 40 60 550 1.5 50 96 53
               
CA12-50-1064 50 245 1064 4.9 50 490 35
CA12-50-808 50 145 808 2.9 50 290 37
CA12-50-700 50 110 700 2.2 50 220 39
CA12-50-600 50 100 600 2 50 200 40
               
CA12-70-750 70 120 750 1.7 50 336 29
CA12-70-650 70 105 650 1.5 50 294 30
CA12-70-550 70 91 550 1.3 50 255 32

 

 

 

Composition

These nanoparticles are shipped in 18MEG DI water (default solution) with no measurable residual reactants.

Custom Formulation

Please contact us.

Quantity

This product is available in 1mL volumes and larger.

Introductory Kits

Please contact us.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a number of different covalent options.

Shelf Life/Storage Temperature

This product is shipped in a cooler. This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

For post sterilization testing, please choose Sterilization Certification.

For endotoxin purification, choose Endotoxin Purified.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Spherical Gold Nanoparticles UV VIS

How much thickness does your proprietary in vitro polymer bridge add?

From 1-2nm.

What is the length of the different PEGs?

Go to PEG length.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

What is the shelf life if I never open the package and keep refrigerated?

Many years.

Should I store in the refrigerator?

Yes.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

How does your polymer bridge compare to PEG?

It is superior in its resistance to salt, pH, loading, and other chemicals.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

Imaging of Hsp70-positive tumors with cmHsp70. 1 antibody-conjugated gold nanoparticles
MK Gehrmann, MA Kimm, S Stangl… - International journal …, 2015 - ncbi.nlm.nih.gov
… with a diameter of 30 nm were used (Nanopartz, Loveland, CO, USA). After washing away
nonbound antibodies, vacant binding sites on the gold nanoparticles were saturated by bovine
serum albumin (Sigma-Aldrich, St Louis, MO, USA). Antibody-conjugated gold …

Antibody-conjugated gold nanoparticles integrated in a fluorescence based biochip
J Ljungblad - 2009 - diva-portal.org
… antibodies, they can be chemically modified to serve this purpose … Primary amines present in
an introduced antibody react and covalently bind to the ester, without addition of a spacer … Citrate
stabilized and PEG conjugated gold nanoparticles were bought from Nanopartz, Salt …

Label-free capture of breast cancer cells spiked in buffy coats using carbon nanotube antibody micro-arrays
F Khosravi, P Trainor, SN Rai, G Kloecker… - …, 2016 - iopscience.iop.org
… The ester on the other end of the molecule provided an attachment point for antibodies. PASE
(AnaSpec, Cat. No … Instead of using anti-EpCAM, an antibody conjugated gold NPs (Nanopartz,
Cat. No. C11-15-TX-50) were used to functionalize with the PASE linker molecule …

Helium beam shadowing for high spatial resolution patterning of antibodies on microstructured diagnostic surfaces
E Cacao, T Sherlock, A Nasrullah… - …, 2013 - biointerphases.springeropen.com
… Protein patterning by helium-beam antibody modification followed by BSA passivation … binding
of gold nanoparticle probes (colloidal gold conjugated with antibodies) [30–32] or gold
nanoparticle … nanoparticle probes (20-PC-40 or 20-PC-100, both from Nanopartz Inc., Loveland …

Tip-enhanced Raman detection of antibody conjugated nanoparticles on cellular membranes
KD Alexander, ZD Schultz - Analytical chemistry, 2012 - ACS Publications
… Complete details are available in the Supporting Information. Antibody conjugation
was performed by standard EDC coupling using a conjugation kit (Nanopartz, Inc.)
and characterized as described in the Supporting Information …

EGFR antibody conjugated bimetallic Au@ Ag nanorods for enhanced SERS-based tumor boundary identification, targeted photoacoustic imaging and …
S Chen, C Bao, C Zhang, Y Yang, K Wang… - Nano Biomed …, 2016 - nanobe.org
… resuspended in 10 mM borate buffer and reacted with mouse monoclonal anti-EGFR antibodies
at room … Au@Ag NR nanotags) were washed twice by centrifugation to remove free antibody, and
re … absorbing at 808 nm (Nanopartz, Loveland, CO, USA) were used at 18 μg mL− 1 …

Detecting Neurotransmitters Using Resonant Nanoparticles
E Forati, A Sabouni, S Ray, D Sievenpiper - ebrahimforati.com
… objective lens a) gold nanoparticles coated with the antibody beam splitter … We determined the
concentration using Bradford assay and then we conjugated anti-dopamine antibodies with
nano-rods bought from Nanopartz Inc. using their established protocol …

Targeting small molecule drugs to T cells with antibody-directed cell-penetrating gold nanoparticles
YSS Yang, KD Moynihan, A Bekdemir… - Biomaterials …, 2019 - pubs.rsc.org
… PEGylated gold nanoparticles (2.1 ± 0.86 nm) coated by thiol-terminated poly(ethylene glycol)
(molecular weight 282.35 g mol −1 ) were purchased from NanoPartz™ … We hypothesized that
by conjugating amph-NPs to antibodies or antibody fragments – which would …

Development of gold nanorod lateral flow test for quantitative multi-analyte detection
M Venkataramasubramani, L Tang - … Conference 2009, 15–17 May 2009 …, 2009 - Springer
… peak, axial size: 10 nm, longitu- dinal: 35 nm (TEM data is courtesy of Nanopartz) … Antibodies
solution was then added and incubated for 1 hr under constant sonication at room … Spectroscopy
Studies: Absorption spectra of gold nanorods and the monitoring of antibody-gold conju …

 

The manufacturing method for these nanoparticles are based on our proprietary method. This family of products have resulted in more than 100 publications and is the cornerstone of our product line. Other covalent polymer bridges are available as well.

 

 

  Nanopartz™ Ab Conjugated Gold Nanoparticles Others
Stability (salt,pH,chemicals) High Medium
Monovalent option Yes No
Nonspecific binding Very low Medium
Sterilization Yes No

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized

Example part number is CAS11-10-488-Host Ab-Anti Reactivity-1KPEG-FC-PBS-50-1-CS-EP where:

CAS11 - Product family number for Secondary Antibody Conjugated Spherical Gold Nanoparticles. For Gold Nanorods, its CAS12 and so forth.

10 - Sphere diameter in nanometers. Other choices are 1.8 to 1500.

488 - Optional Fluorophore.

Host Ab - Secondary Antibody Host.

Anti Reactivity - Targeted Species (ie Anti Human for targeting Human IgG).

Polymer Bridge - From adsorbed to 30KDa Lengths.

Fc- Optional Fc binding to the Antibody leaving the Fab region untouched.

DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS

50 - Optical Density, in this case OD=50, optional 250

1 - Volume (mL). Other choices are 5mL, 10mL and more.

CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification.

EP - Endotoxin Purified - The product is endotoxin purified and certified.

How to Order

Ordering by scrolling down and selecting the options from the selection below.

 

Home
» CA- Antibody Gold Nanoparticles
CA11 Primary Antibody Functionalized Spherical Gold Nanoparticles
CA11 Primary Antibody Functionalized Spherical Gold Nanoparticles
Part Number: CA11

Options: -(SIZE)-(FLUOROPHORE)-(HOST)-(REACTIVITY)-(POLYMER BRIDGE LENGTH)-(BUFFER)-(OD)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL)-(LYO)-(MONO)-(FC) Example part number for a 30nm diameter sphere, 550nm fluorophore, EGFR, 2KPEG polymer bridge, Fc attachment, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CA11-30-550-EGFR-2K-DIH-50-1-FC
Covalent polymer bridge length - the distance between the gold nanoparticle and the Ab attachment
NPB - Nanopartz Proprietary Bridge is 1-2nm in length)
PEG lengths are shown Here
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$0.00
CAS11 Secondary Antibody Functionalized Spherical Gold Nanoparticles
CAS11 Secondary Antibody Functionalized Spherical Gold Nanoparticles
Part Number: CAS11

Options: -(SIZE)-(FLUOROPHORE)-(HOST)-(REACTIVITY)-(POLYMER BRIDGE LENGTH)-(BUFFER)-(OD)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL)-(LYO) Example part number for a 30nm diameter sphere, 550nm fluorophore, Goat anti Human IgG, 2KPEG polymer bridge, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CAS11-30-550-Rabbit-AntiHuman-2K-DIH-50-1
Covalent polymer bridge length - the distance between the gold nanoparticle and the Ab attachment
NPB - Nanopartz Proprietary Bridge is 1-2nm in length)
PEG lengths are shown Here
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$440.00
CA12 Primary Antibody Functionalized Gold Nanorods
CA12 Primary Antibody Functionalized Gold Nanorods
Part Number: CA12

Options: -(DIAM/SPR)-(FLUOROPHORE)-(HOST)-(REACTIVITY)-(POLYMER BRIDGE LENGTH)-(BUFFER)-(OD)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL)-(LYO)-(MONO)-(FC) Example part number for a 10nm diameter nanorod with a peak SPR=850nm, 550nm fluorophore, anti EGFR IgG, 2KPEG polymer bridge, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CAS12-10-850-550-EGFR-2K-DIH-50-1
DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
Covalent polymer bridge length - the distance between the gold nanoparticle and the Ab attachment
NPB - Nanopartz Proprietary Bridge is 1-2nm in length)
PEG lengths are shown Here
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$0.00
CAS12 Secondary Antibody Functionalized Gold Nanorods
CAS12 Secondary Antibody Functionalized Gold Nanorods
Part Number: CAS12

Options: -(DIAM/SPR)-(FLUOROPHORE)-(HOST)-(REACTIVITY)-(POLYMER BRIDGE LENGTH)-(BUFFER)-(OD)-(VOL)-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL)-(LYO)-(MONO)-(FC) Example part number for a 10nm diameter nanorod with a peak SPR=850nm, 550nm fluorophore, Goat anti Human IgG, 2KPEG polymer bridge, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CAS12-10-850-550-Rabbit-AntiHuman-2K-DIH-50-1
DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
Covalent polymer bridge length - the distance between the gold nanoparticle and the Ab attachment
NPB - Nanopartz Proprietary Bridge is 1-2nm in length)
PEG lengths are shown Here
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$480.00