Nanopartz Spherical Gold Nanoparticles for Gene Gun and CRISPR Applications

Nanopartz has developed highly monodisperse Spherical Gold Nanoparticles with a wide range of covalent and adsorbed ligands specifically for Gene Gun and CRISPR applications. These Gold Nanoparticles range in size from 500-1500nm and are perfectly spherical, resulting in significantly reduced damage to cells. Standard adsorbed ligands include PEI, citrate, PAH, and more. Standard covalent ligands include PEG, alkyne, amine, azide, biotin, carboxyl, hydroxyl, methyl, maleimide, neutravidin, nhs, streptavidin, Protein A and G, Thiol, and secondary antibodies. Other options include oligos, DNA, and plasmids. These particles are guaranteed not to aggregate. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. Typical volume is 1mL at a concentration of 0.25g. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the target is determined.

A11-1500 11Y194 1.5um web

 

 

Part # Diameter (nm) Peak SPR Wavelength (nm) NPS/ml Molarity (pM) Moles Wt. conc (g/ml) Wt. % IgG Ab/np Total (ug) IgG
PG11-500 500 600 1.99E+11 3.30E+02 3.31E-13 0.25 25.00% 10000 496
PG11-1000 1000 610 2.48E+10 4.10E+01 4.14E-14 0.25 25.00% 40000 248
PG11-1500 1500 620 7.35E+09 1.20E+01 1.23E-14 0.25 25.00% 90000 165

 

Functionalized Nanorod Block Invitro

 

Citrate capped Spherical Gold Nanoparticles

 

MW (g/mole)
Zeta Charge (mV)
Salt resistant?
Concentration Sensitive?
Easy to adsorb proteins?
Easy to thiolate?
Shelf Life at 4C
Citrate
192
-35
1.5mM
Yes
Yes
Yes
6 months
Nanopartz Carboxylic Acid
212
-30
15mM
Less than Citrate
Better than Citrate
Better than Citrate
years
PEI
100K
30
150mM
No
Yes
Yes
6 months
PAA
10K
-30
150mM
No
Yes
Yes
6 months
PAH
10K
20
150mM
No
Yes
Yes
6 months
PVP
40K
-10
0.5M
No
No
Yes
6 months
CTAB
364
35
0.5M
Yes
No
No
6 months
BSA
66K
-15
0.5M
No
No
No
6 months

 

 

Functionalized Nanorod Block Invitro

 

PEG Functionalized Spherical Gold Nanoparticles

 

 

Loading Range (nm2)
Charge (mV)
Smaller particles Larger particles Smaller particles Larger particles
PEG
3
1
-27
-12
Alkyne
4
2
-25
-25
Amine
4
2
-20
20
Azide
4
2
-25
-25
Biotin
3
1.5
-25
-15
Carboxyl
4
2
-38
-45
Methyl
4
2
-27
-12
Streptavidin
0.01
0.01
-25
-10
Neutravidin
0.01
0.01
-25
-10
GSH (Glutathione)
3
1.5
-25
-15
Protein A
0.04
0.04
-25
-10
Protein G
0.04
0.04
-25
-10
Maleimide
4
2
-27
-12
NHS
3
1.5
-25
-15
Hydroxyl
4
2
-27
-12
Thiol
5
5
-30
-30
Galactose
2
2
-30
-30
Mutab
5
5
30
40
Secondary IgG
0.02
0.02
0.02
0.02

 

Composition

These nanoparticles are shipped in 18MEG DI water (default solution) with no measurable residual reactants.

Custom Formulation

Please contact us.

Quantity

This product is available in 1mL volumes and larger.

Introductory Kits

Please contact us.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a number of different covalent options.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

For post sterilization testing, please choose Sterilization Certification.

For endotoxin purification, choose Endotoxin Purified.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

How much thickness does your proprietary capping agent add?

Anywhere from 1-5nm.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.

Can I resuspend in water?

Yes

What is the shelf life if I never open the package and keep refrigerated?

Many years.

Should I store in the refrigerator?

Yes.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

How does your polymer bridge compare to PEG?

It is superior in its resistance to salt, pH, and other chemicals.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

Enhancing sensitivity of surface plasmon resonance biosensors by functionalized gold nanoparticles: size matters

T Špringer, ML Ermini, B Špačková… - Analytical …, 2014 - ACS Publications
... resistivity, Direct-Q from Millipore). Gold Nanoparticles Spherical AuNPs
covalently functionalized with neutravidin via a 2 nm polymer bridge were
purchased from Nanopartz Inc., USA. The diameters of the gold ...

 

Physisorption of functionalized gold nanoparticles on AlGaN/GaN high electron mobility transistors for sensing applications

MS Makowski, S Kim, M Gaillard, D Janes… - Applied physics …, 2013 - aip.scitation.org
... used to measure electrical characteristics of physisorbed gold nanoparticles (Au NPs)
functionalized with alkanethiols ... linkers to the gate area of AlGaN/GaN HEMTs include patterning
gold at the ... Also, the effectiveness of the UV functionalization method is offset by the limited ...

 

[HTML] Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis …

PM Tiwari, E Eroglu, SS Bawage, K Vig, ME Miller… - Biomaterials, 2014 - Elsevier
... Carboxyl-polymer coated spherical GNPs (Nanopartz™, Loveland, CO, USA) 13 nm in size ... For
fluorescence microscopy, FITC conjugated VG-21 peptide was used for functionalization... GNPs
functionalized with FITC-conjugated VG-21 were used to study internalization of the ...

 

Wide‐field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy

S Patskovsky, E Bergeron, D Rioux… - Journal of …, 2015 - Wiley Online Library
... ON) were tethered to AuNPs (50 µg/mL, 100 nm diameter, Nanopartz, A11-100 ... Some examples
of experimental spectral curves for single 100 nm functionalized AuNPs are presented in ... shifted
for about 6 nm that could be explained by AuNPs functionalization and attachment to ...

 

Size dependence of Au NP-enhanced surface plasmon resonance based on differential phase measurement

S Zeng, X Yu, WC Law, Y Zhang, R Hu, XQ Dinh… - Sensors and Actuators B …, 2013 - Elsevier
... OD = 1.0) with diameters 40 nm, 60 nm, 70 nm and 80 nm were purchased from Nanopartz
Accurate. ... The system was left for a period of 20 min and then rinsed by DI water to confirm the
functionalization... A review on functionalized gold nanoparticles for biosensing applications. ...

 

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN Afrooz, IA Khan, SM Hussain… - … science & technology, 2013 - ACS Publications
... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs) in ... with 10 nm primary particle diameter were procured from Nanopartz
Inc. ... PA-SWNT Preparation To perform surface functionalization with pluronic acid (PA) F127 ...

 

Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

T Špringer, J Homola - Analytical and bioanalytical chemistry, 2012 - Springer
... a mixture of carboxy-terminated and hydroxy-terminated thiols were obtained from Nanopartz,
USA, at ... During the functionalization processes described above, the flow rate and the temperature
were set ... BSA was flowed along the surface of an SPR chip functionalized with Ab1 ...

 

Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles

M Mancuso, L Jiang, E Cesarman, D Erickson - Nanoscale, 2013 - pubs.rsc.org
... Gold and silver nanoparticle functionalizationGold and silver particles with average diameters
of 15 and 20 nm, respectively, were functionalized using thiol based chemistry using methods
described by others. ... Gold nanoparticles were purchased from Nanopartz (Loveland, CO ...

 

[HTML] Plasmonic enhancement of dye sensitized solar cells via a tailored size-distribution of chemically functionalized gold nanoparticles

C Andrei, E Lestini, S Crosbie, C de Frein, T O'Reilly… - PLoS …, 2014 - journals.plos.org
... particles with a tailored size distribution and commercially available (spherical) AuNPs Nanopartz,
wt 1.75 ... nm), with optically dispersing TiO 2 particles (Ø ∼190 nm) and functionalized with AuNPs ...
TiO 2 functionalisation with AuNPs) and Figure 4 (after the functionalization) it can ...

The manufacturing method for these nanoparticles are based on our proprietary method. This family of products have resulted in more than 1000 publications and is the cornerstone of our product line. Other covalent polymer bridges are available as well. Please follow the menu to find options from 1 carbon to 30KDa length covalent polymer bridges.

 

This product is for research use only and is not intended for diagnostic use or to treat, cure or prevent any disease. The product is not available for resale. The safety of this material has not been determined and we recommend that this product and components are handled only by persons trained in laboratory techniques and are used in accordance with good laboratory practice. As all chemicals should be considered as potentially hazardous it is advisable when handling chemical reagents to wear suitable protective clothing. The BiolisticTM process is covered by patents owned by Dupont, Inc. and purchase of these products does not convey a license under these patents.

 

 

  Nanopartz™ Gene Gun Gold Nanoparticles Existing Technology
Monodispersity High Low
% Spheres 100% Low
Many ligands to choose from High One
Sterilization Yes No

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized

Example part number is PG11-1500-PEI-DIH-0.25-1-CS-EP where:

PG11 - Product family number for Gene Gun Spherical Gold Nanoparticles

1500 - Sphere diameter in nanometers. Other choices are 500 to 1000

PEI- Adsorbed ligand, in this case PEI.

DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS

0.25 - Wt. in grams

1 - Volume (mL). Other choices are 5mL, 10mL and more.

CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification.

EP - Endotoxin Purified - The product is endotoxin purified and certified.

How to Order

Ordering by scrolling down and selecting the options from the selection below.

 

Home » P - Applications » PG- Gold Nanoparticles for Gene Guns
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Gold Nanoparticles for Gene Guns
Gold Nanoparticles for Gene Guns
Part Number: PG11
Options: (DIAMETER)-(CAPPING AGENT)-(BUFFER)-(Wt)-(VOL)-CS-EP
Example part number for a 1500nm diameter sphere, PEI capping agent, in water, 0.25g at 1mL, with sterilization certificate, no endotoxin purification would be: PG11-1500-PEI-DIH-0.25-1-CS
 Spherical Gold Nanoparticle Diameters from 500nm to 1500nm
 CIT (Citrate)
NPC (Nanopartz Carboxylic Acid
PEI (100K Polyethylenimine)
PAH (25K Polyallylamine hydrochloride)
PAA (10K Polyacrylic acid)
PVP (40K Polyvinylpyrrolidone)
BSA (Bovine Serum Albumin)
CTAB (Cetyltrimethylammonium bromide)
Tannic Acid
SDS
 DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
 Wt in grams
 For volumes larger than 5mL, please contact us
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Price: $670.00
Covalent Functionalized Spherical Gold Nanoparticles for Gene Guns
Covalent Functionalized Spherical Gold Nanoparticles for Gene Guns
Part Number: PGC11
Options: (DIAMETER)-(FUNCTIONALIZATION)-(BUFFER)-(Wt.)-(VOL)-CS-EP
Example Part Number: 1500nm diameter spherical gold nanoparticle for gene guns, carboxyl polymer, 18MEG DD DI Water, 0.25g, in 1mL, Endotoxin Purified
C11-1500-TC-DIH-0.25-1
 Spherical Gold Nanoparticle Diameters from 500nm to 1500nm
 Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
 DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
 Wt in grams
 For volumes larger than 5mL, please contact us
Sterilization Product comes sterilized. This option provides a complete post testing of sterilization along with a Certificate of Sterilization
CS (Certified Sterilized) (+$150.00)
Endotoxin Product is Endotoxin purified and then tested for Endotoxin. An Endotoxin Certificate is provided.
EP (Endotoxin Purified and Certified) (+$250.00)
Price: $840.00