cellswithmicrorods with inset

Nanopartz™ Gold Nanoparticles for in vitro Bright Field Imaging

Nanopartz™ Gold Nanoparticles for in vitro Bright Field Imaging utilize the complete line of microgold and nanowires for bright field microscopy. These conjugated particles exhibit true white light scattering - many orders of magnitude greater than quantum dot fluorescence. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. This product comes in one concentration, 0.25mg/mL. Ligands include our own proprietary MUTAB, providing a covalent, positive charged particles which has shown in literature to exhibit strong cellular uptake.

 

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Conjugated Microgold for In-Vitro
Conjugated Microgold for In-Vitro
C13-(LENGTH)-(CONJUGATION); 0.25mg in 1mL
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
Conjugated Gold Nanowires for In-Vitro
Conjugated Gold Nanowires for In-Vitro
C14-(LENGTH)-(CONJUGATION); 0.25mg in 1mL
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
CF13 Fluorophore Labeled Microgold
CF13 Fluorophore Labeled Microgold
Part Number: CF13-(LENGTH)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(Buffer)-5-(Sterilization)-(Endoxtoxin)-(Negative Control). Example part number for a 1000nm long Microgold rod, 550 fluorophore, with a methyl polymer conjugation, in water, at 5 OD-mLs (2.5mg/mL, OD=50, 0.1mL) would be: CF13-1000-550-TM-DIH-5
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
CF14 Fluorophore Labeled Gold Nanowires
CF14 Fluorophore Labeled Gold Nanowires
Part Number: CF14-(LENGTH)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(Buffer)-5-(Sterilization)-(Endoxtoxin)-(NEGATIVE CONTROL). Example part number for a 10000nm long gold nanowire, 550 fluorophore, with a methyl polymer conjugation, in water, at 5 OD-mLs (2.5mg/mL, OD=50, 0.1mL) would be: CF14-10000-550-TM-DIH-5
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
PEG Microgold
PEG Microgold
Part Number: CP13-(LENGTH)-(FUNCTIONALIZATION)-(PEG MW)-(Buffer)-5-(Sterilization)-(Endoxtoxin). Example part number for a 1000nm long Microgold rod, with a 5k Methyl PEG conjugation, in water, at 5 OD-mLs (2.5mg/mL, OD=50, 0.1mL) would be: CP13-1000-PM-5K-DIH-5
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Price: $480.00
PEG Gold Nanowires
PEG Gold Nanowires
Part Number: CP14-(LENGTH)-(FUNCTIONALIZATION)-(PEG MW)-(Buffer)-5-(Sterilization)-(Endoxtoxin). Example part number for a 10000nm long gold nanowire, with a 5k Methyl PEG conjugation, in water, at 5 OD-mLs (2.5mg/mL, OD=50, 0.1mL) would be: CP14-10000-PM-5K-DIH-5
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Price: $480.00

 

 

 

Part # Diameter (nm) Length (nm) Aspect Ratio Peak SPR Wave (nm) SPR OD (1cm) Microgold Vol (nm3) Microgold /mL Wt. conc (ug/ml) Wt. % PPM Molarity (pM) SPR Molar Ext. (M-1cm-1)
CB13-500 75 500 6.7 510 0.25 2.10E+06 1.24E+09 50 0.01% 50 2.06 1.21E+08
CB13-1000 100 1000 10 510 0.25 7.59E+06 3.42E+08 50 0.01% 50 0.57 4.39E+08

 

 

 

 

Part # Diameter (nm) Length (nm) Aspect Ratio Peak SPR Wave (nm) Nanowires Vol (nm3) Nanowires /mL Wt. conc (ug/ml) Wt. % PPM Molarity (pM) SPR Molar Ext. (M-1cm-1)
CB14-1000 75 1000 13 510 4.31E+06 3.01E+09 250 0.03% 250 5.02 9.96E+06
CB14-2000 75 2000 27 510 8.72E+06 1.49E+09 250 0.03% 250 2.48 2.02E+07
CB14-4000 75 4000 53 510 1.76E+07 7.39E+08 250 0.03% 250 1.23 4.06E+07
CB14-6000 75 6000 80 510 2.64E+07 4.91E+08 250 0.03% 250 0.82 6.10E+07
CB14-10000 75 10000 133 510 4.40E+07 2.94E+08 250 0.03% 250 0.49 1.02E+08

 

 

 

 

Functionalized Nanorod Block Invitro

mutab

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer (top) and Nanopartz™ covalent MUTAB (bottom). Thickness of polymer is less than the size variablity of the gold nanoparticle.

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

Composition

These in vitro gold nanoparticles for in vitro Bright field imaging come in numerous conjugations:

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

  1. Amine
  2. Biotin
  3. Carboxyl
  4. Methyl
  5. Neutravidin
  6. Streptavidin
  7. Azide
  8. MUTAB (a strongly positive non cytotoxic CTAB replacement)

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM
  6. Protein A

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

These gold nanoparticles exhibit white light scattering in brightfield microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

 

 

  Nanopartz™ Gold Nanoparticles for BrightField Imaging Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

 

  • Extremely stable. Shelf life is greater than six months.
  • Covalent bonds.
  • High anisotropy.
  • Low cytotoxicity. Gold is FDA approved for rheumatoid arthritis.
  • Excellent photostability
  • Buffer Stability - stable from pH 4-9.
  • Instrument capability - any brightfield microscope

Spherical Gold UV VIS

Microgold (Part# C13-1000-MUTAB) with MUTAB coating under brightfield microscope, cancer cell, 100x objective. Inset shows individual microgold

Gold Nanorods UV VIS

Gold Nanowires (Part# C14-4000) under brightfield microscope, 40x objective

 

 

 

"We were previously making our own gold nanoparticles using the commonly used technique of citrate reduction of Au(III). The colloids generated are broadly polydisperse, and we were concerned that our application of these particles in immuno-gold labeling of cells for optical coherence microscopy (OCM) would be utilizing only the large diameter tail of the distribution. We purchased the sampler package of NanoPartz gold spheres (30 nm, 50 nm, 70 nm, 90 nm) and examined each size carefully with dynamic light scattering, OCM, and spectrophotometry. Our measurements indicated diameters a couple nanometers larger than specifications (a result of measurements on the fully hydrated state?), and remarkable monodispersity - within specs. It is clear to us now that our early immuno-gold results were dominated by the largest diameter spheres of our early colloids. We are delighted by the NanoPartz monodisperse preparations and recommend them strongly!"

Dr. Richard C. Haskell 
Professor of Physics & Director of Physics Clinic 
Physics Department 
Harvey Mudd College



"The scientists at Nanopartz have developed a line of colloidal gold nanorods with extremely high monodispersity in size, shape, and mass. The surfactant layer is highly stable and readily displaced for biofunctionalization. In every test, in vitro and in vivo, these materials have exceeded our expectations."


Geoffrey A. von Maltzahn
NSF and Whitaker Graduate Fellow
Harvard-MIT Division of Health Sciences and Technology
Laboratory for Multiscale Regenerative Technologies

 

The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts

DS Wagner, NA Delk, EY Lukianova-Hleb, JH Hafner… - Biomaterials, 2010 - Elsevier
... the unique optical properties of PNBs that turned out to be much brighter than gold ... and quantified
four independent optical signals in vitro and in vivo: bright field image (characterized ... receptor
(EGFR) antibody C225 were fabricated using covalent conjugation by Nanopartz Inc. ...

 

Generation and detection of plasmonic nanobubbles in zebrafish

EY Lukianova-Hleb, C Santiago, DS Wagner… - …, 2010 - iopscience.iop.org
... sample chamber were mounted on the stage of an inverted optical microscope; PNB generation ...
epidermal growth factor receptor (EGFR) antibody C225 that were obtained from Nanopartz Inc. ...
detection of PNB in gold NP-treated A 549 cells: (a) bright field microscopy image of ...

 

Shape-dependent dispersion and alignment of nonaggregating plasmonic gold nanoparticles in lyotropic and thermotropic liquid crystals

Q Liu, J Tang, Y Zhang, A Martinez, S Wang, S He… - Physical Review E, 2014 - APS
... Comparatively large, polymer coated GNRs (Nanopartz Inc.) with a mean diameter of 40 nm and
mean length ... Polarizing and bright-field optical imaging of GNR-LC composites was performed
using an Olympus BX-51 upright ... (b, c) The corresponding brightfield micrographs for ...

 

Portable mercury sensor for tap water using surface plasmon resonance of immobilized gold nanorods

EC Heider, K Trieu, AFT Moore, AD Campiglia - Talanta, 2012 - Elsevier
... 6.29×10 11 nanorods/mL), and 750 nm (5.77×10 10 nanorods/mL) were purchased from
Nanopartz, Inc. ... Images of the surface were acquired using scanning electron microscopy... A bright
field illumination image of the resulting surface after ∼24 h of immersion is shown in Fig. ...

 

Differential interference contrast microscopy imaging of micrometer-long plasmonic nanowires

JW Ha, K Chen, N Fang - Chemical Communications, 2013 - pubs.rsc.org
... Bright field microscopy typically generates low contrast for most biological samples as few absorb
to ... Scattering-based dark field microscopy may suffer from the relatively high scattering background ...
Au nanowires used here were purchased from Nanopartz (A14-2000, Loveland ...

 

Imaging of epidermal growth factor receptor on single breast cancer cells using surface-enhanced Raman spectroscopy

L Xiao, S Harihar, DR Welch, A Zhou - Analytica chimica acta, 2014 - Elsevier
... purchased from commercial source and were used as received: gold nanorods (5.1 × 10 11
particles mL −1 , Nanopartz Inc., USA ... 5c–f) since GNR scatter light intensely and they are much
brighter than cells in the dark field [45 ... The presence of many bright spots on 435 cells (Fig ...

 

Waveguide scattering microscopy for dark-field imaging and spectroscopy of photonic nanostructures

DJ Hill, CW Pinion, JD Christesen, JF Cahoon - Acs Photonics, 2014 - ACS Publications
... Dark-field microscopy (DFM) is ... of a sample at oblique angles and collection of the scattered light
by an objective lens, producing a bright object surrounded ... and DFM (middle left), S-WSM (middle
right), and C-WSM (far right) optical images with a 10× microscope objective; all ...

 

Wavelength-dependent differential interference contrast microscopy: selectively imaging nanoparticle probes in live cells

W Sun, G Wang, N Fang, ES Yeung - Analytical chemistry, 2009 - ACS Publications
... can be detected by a variety of optical microscopy techniques, including bright-field and
dark-field ... Dark-field microscopies have gained much attention due to their high signal strength
and ... that were used to conjugate with TAT peptide were purchased from Nanopartz (Salt Lake ...

 

Shape-dependent oriented trapping and scaffolding of plasmonic nanoparticles by topological defects for self-assembly of colloidal dimers in liquid crystals

B Senyuk, JS Evans, PJ Ackerman, T Lee, P Manna… - Nano …, 2012 - ACS Publications
... By means of dark-field microscopy and polarization-sensitive two-photon luminescence (TPL)
imaging, we ... with respect to the neighboring microparticle and the uniform far-field LC alignment. ...
akyl acrylate) polymer conjugation (Figure 1a–d) were obtained from Nanopartz, Inc. ...

 

Optical microscopy of soft matter systems

T Lee, B Senyuk, RP Trivedi… - Fluids, Colloids and Soft …, 2016 - books.google.com
... lens Light source (a) (b) 20 μm 10 μm (c) Figure 10.2 Bright field microscopy diagram (a ... Source:
TEM image courtesy of Nanopartz Inc.(See color plate section for the color representation of ... of
the sample having dif- ferent refractive indices appear darker or brighter compared to ...