Nanopartz Sodium Azide Purification Kit
Nanopartz sodium azide purification kit is specifically for purifying antibodies stored in sodium azide. No columns or tubes or dialysis are necessary. No special handling of liters of sodium azide contaminated water created in purification. 99% antibody retention. 99.99% sodium azide purification. The video below shows how this can be achieved in less than 30 seconds. The key to the product is the sodium azide reactive ultra high loading Nanopartz nanomagnets. Highly reactive, highly magnetic, and yet small enough to purify the antibody solution of sodium azide in a matter of seconds. One kit comes with instructions and enough nanomagnets for 10 purifications.
Video of 30 second Sodium Azide Purification Kit in Action
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(A) Ab solution with Sodium Azide. (B) Add sodium azide reactive nanomagnets. (C) Use external magnet to pull out nanomagnets loaded with sodium azide (D) Remove solution of purified Antibodies. |
| Nanopartz Kit | Dialysis | Columns | |
|---|---|---|---|
| Time | < 30 seconds | 12 hours | 30 minutes |
| Cost | Low | Medium (TFF tubes) | Medium |
| Residual Cleanup (Sodium Azide) | 1mL (bound non hazardous material) | Liters (hazardous sodium azide dilutent) | 10mL (hazardous sodium azide dilutent) |
| Product Loss (Ab efficiency) | Very Low Loss | High Loss | Highest Loss |
Table 1. Comparison of Technologies
Composition
These spherical magnetic nanoparticles are shipped in DI water with no measurable residual reactants.
Quantity
This kit is available in 1mL volumes and larger.
Delivery
Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.
Functionalization
This product comes functionalized with an sodium azide reactive polymer.
Shelf Life/Storage Temperature
This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.
Toxicity
This product is known to be noncytotoxic. This product has been sterilized and is biological and shipped in DNase free water.
Sterilization
This product is sterilized.
Certifications
Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for mass measurements
TEM (Phillips CM-100 100KV) for sizing
How long does it take to bind 99.99% of the sodium azide onto the magnets?
About 15 seconds in a 1000 rpm vortexer.
How long does it take to pull the sodium azide bound nanomagnets to the side of the centrifuge tube?
About 15 seconds using our Nanopartz Nanomagnet.
Does this affect the antibody health?
Absolutely not.
What is the purification rate?
About 99.99%
Be the First!
Be the First!
This product replaces other methods of sodium azide purification including dialysis and columns. EPA regulates the amount of sodium azide that can be disposed of through drains and in most cases, current methods of dialysis and columns exceed the EPA limitation. If properly handled, dialysis can result in liters of sodium azide contaminated solutions that is required to be handled as hazardous waste. Our product results in 1mL of bound sodium azide which may be disposed of as a solid non hazardous waste component.
| Nanopartz Kit | Dialysis | Columns | |
|---|---|---|---|
| Time | < 30 seconds | 12 hours | 30 minutes |
| Cost | Low | Medium (TFF) | Medium |
| Residual Cleanup (Sodium Azide) | 1mL | Liters | 10mL |
| Product Loss (Ab efficiency) | Low | Terrible | Terrible |
- Fast!
- No resultant hazardous waste. Total volume of waste is 1mL vs. liters of hazardous waste created with dialysis.
- High Product throughput (low Antibody loss).
- Better resultant assays - Sodium Azide is toxic to the antigens which antibodies bind.
Example part number is PPZ-KIT-10 where:
PPZ - Product family number for Nanopartz Sodium Azide Purification Kit
KIT - Includes reagents and instructions
10 - Amount in mg of magnetic material. Other options are 25 and 50. 1mg is necessary for efficient removal of one lot.
Ordering by scrolling down and selecting the options from the selection below.