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Nanopartz™ Plasmonic Fluorophores™

Nanopartz™ Plasmonic Fluorophores™ are fluorophore decorated gold nanorods manufactured using multiple patent pending and proprietary methods that result in fluorophores that exhibit much higher intensities than conventional fluorophores, no photobleaching even under the highest laser intensities, with chemical resistance to extreme pHs, salts, and other adverse chemicals. Peak fluorescence range from 550nm to 800nm, where each nanorod fluoresces up to 50,000 times brighter than a single fluorophore. Every batch is completely characterized including size, monodispersity, aggregation, and concentration. Residual chemicals are reduced to trace. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Conjugation options include amine, biotin, carboxyl, methyl, neutravidin, nhs, maleimide, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. These conjugated particles can be used in darkfield, fluorescence, and other imaging modes; ELISA, lateral flow, and other assays.

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Conjugated In-Vitro Plasmonic Fluorophores
Conjugated In-Vitro Plasmonic Fluorophores
Part #C16-(FLUOR)-(CONJUGATION)-(OD-mLs)
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $440.00

 

 

 

 

Part #  Diam (nm) Long Size (nm) Peak SPR Wave (nm) Ex/Em (nm) Wt. conc (mg/ml) (10-3) Wt. % PPM Molarity (pM) Spectrally Similar Dyes
C16-10-1064 10 67 1064 777/794 1750.0 0.1750% 1750 32.9 IRDye 800
C16-10-980 10 59 980 777/794 1750.0 0.1750% 1750 38.6 IRDye 800
C16-10-850 10 45 850 777/794 1750.0 0.1750% 1750 53.0 IRDye 800
C16-10-808 10 41 808 777/794 1750.0 0.1750% 1750 60.3 IRDye 800
C16-10-780 10 38 780 754/776 1750.0 0.1750% 1750 66.4 Alexa Fluor 750
C16-25-800 25 99 800 777/794 2500.0 0.2500% 2500 5.8 IRDye 800
C16-25-750 25 86 750 754/776 2500.0 0.2500% 2500 7.0 Alexa Fluor 750
C16-25-680 25 68 680 692/712 2500.0 0.2500% 2500 9.9 Alexa Fluor 680, Cy5.5
C16-25-650 25 60 650 652/672 2500.0 0.2500% 2500 12.0 Alexa Fluor 647, Cy5
C16-25-633 25 56 633 638/658 2500.0 0.2500% 2500 13.6 Alexa Fluor 633
C16-25-600 25 46 594 593/618 2500.0 0.2500% 2500 19.9 Alexa Fluor 594, Texas Red
C16-25-550 25 33 546 562/576 2500.0 0.2500% 2500 45.6 Alexa Fluor 546, Alexa Fluor 555

 

 

 

 

 

 

mutab

 

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

 

 

Fluorophore Type Excitation (nm) Emission (nm) Spectrally Similar Dyes
800 788 808 IRDye 800
750 750 773 Alexa Fluor 750
680 673 707 Alexa Fluor 680, Cy5.5
650 646 662 Alexa Fluor 647, Cy5
633 638 658 Alexa Fluor 633
600 591 604 Alexa Fluor 594, Texas Red
550 555 570 Alexa Fluor 546, Alexa Fluor 555
532 533 556  
514 520 540  
488 494 518 FITC, Alexa Fluor 488

 

 

 

Composition

These Plasmonic Fluorophores™ come in numerous conjugations:

Available in amine, biotin, carboxyl, methyl, neutravidin, streptavidin, nhs, maleimide, and azide forms. Methyl acts as a free acid form to be used as a non reactive negative control.

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM

Quantity

This product is available in 2.5mg and 12.5mg quantities. Buffer is PBS.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

Unknown. Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Fluorescence (Nanopartz Flurophore I) for fluorescence measurements

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products. >

 

 

  Nanopartz™ Plasmonic Fluorophores Dye Labels
Cell Uptake Yes Difficult
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized
  • Customer can focus on research and not on Nanopartz expertise
  • Quick turnaround

microgold in vitro

Microgold (Part #C13-1000-TMU-5) with MUTAB coating under brightfield microscope, cancer cell, 100x objective

How much thickness does your proprietary capping agent add?

It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solvent you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

No

Will the SPR wavelength shift with different solvents?

The SPR is a function of the index of refraction of the host solvent in which the nanoparticles are suspended. Nanorods are the most sensitive and are therefore a great tool for measuring the index of refraction of the host.

What is the shelf life if I never open the package?

1 year.

Should I store in the refrigerator?

Yes.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

Plasmon-enhanced brightness and photostability from single fluorescent proteins coupled to gold nanorods

JE Donehue, E Wertz, CN Talicska… - The Journal of Physical …, 2014 - ACS Publications
... Here, we use gold nanorods and single-molecule fluorescence detection to achieve
plasmon-enhanced emission from intrinsically fluorescent proteins. ... Overall, our work indicates
that plasmonic particles improve both the brightness and photostability of FPs and extends the ...
 

Fluorescent nanothermometers provide controlled plasmonic-mediated intracellular hyperthermia

LM Maestro, P Haro-González… - …, 2013 - Future Medicine
... (ref Q21521MP; CA, USA), whereas the GNRs were provided by Nanopartz (UT, USA ... MP, Baffou
G, Quidant R. Mapping intracellular temperature using green fluorescent protein. ... Laura Martínez
Maestro. 1 Fluorescence Imaging Group, Departamento de Física de Materiales ...
 

Enhancing the optical excitation efficiency of a single self-assembled quantum dot with a plasmonic nanoantenna

M Pfeiffer, K Lindfors, C Wolpert, P Atkinson… - Nano …, 2010 - ACS Publications
... achieve this degree of control, emitters can be chemically conjugated to the plasmon resonant
structure ... The shallow depth of the emitters enables near-field coupling with plasmonic structures
placed on ... with a diameter of 90 nm as specified by the manufacturer (Nanopartz Inc.). ...
 

The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts

DS Wagner, NA Delk, EY Lukianova-Hleb, JH Hafner… - Biomaterials, 2010 - Elsevier
... Fluorescence of DiI was activated with a continuous laser beam at 532 nm that ... in vivo: bright field
image (characterized morphological structure of the object), fluorescent image (was ... receptor
(EGFR) antibody C225 were fabricated using covalent conjugation by Nanopartz Inc. ...
 

[HTML] Super-resolution of fluorescence-free plasmonic nanoparticles using enhanced dark-field illumination based on wavelength-modulation

P Zhang, S Lee, H Yu, N Fang, SH Kang - Scientific reports, 2015 - nature.com
... Even though many natural and synthetic fluorescent probes have been developed, most of ...
technique demonstrated the potential of super-resolution microscopy with fluorescence-free
material. ... and 80-nm SNP colloidal solutions were purchased from Nanopartz ™ (Salt Lake ...
 

Antibody-conjugated gold nanoparticles integrated in a fluorescence based biochip

J Ljungblad - 2009 - diva-portal.org
... field (Em) around the fluorophore in the ground state can lead to increased fluorescence...
Transmitted electrons are then focused on to a fluorescent screen to form an image. ... Citrate
stabilized and PEG conjugated gold nanoparticles were bought from Nanopartz, Salt ...
 

Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells

J Baumgart, L Humbert, É Boulais, R Lachaine… - Biomaterials, 2012 - Elsevier
... during 3–6 h with the AuNP (8 μg/ml, diameter 100 nm, Nanopartz) in the ... For transfection, the
cells were observed by fluorescence microscopy 48 h after the treatment. ... by software (Image
Pro Plus, Media Cybernetics Inc.), taking into account the brightness of fluorescent cells of ...