Nanopartz™ Plasmonic Fluorophores™
Nanopartz™ Plasmonic Fluorophores™ are fluorophore decorated gold nanorods manufactured using multiple patent pending and proprietary methods that result in fluorophores that exhibit much higher intensities than conventional fluorophores, no photobleaching even under the highest laser intensities, with chemical resistance to extreme pHs, salts, and other adverse chemicals. Peak fluorescence range from 550nm to 800nm, where each nanorod fluoresces up to 50,000 times brighter than a single fluorophore. Every batch is completely characterized including size, monodispersity, aggregation, and concentration. Residual chemicals are reduced to trace. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Conjugation options include amine, biotin, carboxyl, methyl, neutravidin, nhs, maleimide, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. These conjugated particles can be used in darkfield, fluorescence, and other imaging modes; ELISA, lateral flow, and other assays.
|Part #||Diam (nm)||Long Size (nm)||Peak SPR Wave (nm)||Ex/Em (nm)||Wt. conc (mg/ml) (10-3)||Wt. %||PPM||Molarity (pM)||Spectrally Similar Dyes|
|C16-10-780||10||38||780||754/776||1750.0||0.1750%||1750||66.4||Alexa Fluor 750|
|C16-25-750||25||86||750||754/776||2500.0||0.2500%||2500||7.0||Alexa Fluor 750|
|C16-25-680||25||68||680||692/712||2500.0||0.2500%||2500||9.9||Alexa Fluor 680, Cy5.5|
|C16-25-650||25||60||650||652/672||2500.0||0.2500%||2500||12.0||Alexa Fluor 647, Cy5|
|C16-25-633||25||56||633||638/658||2500.0||0.2500%||2500||13.6||Alexa Fluor 633|
|C16-25-600||25||46||594||593/618||2500.0||0.2500%||2500||19.9||Alexa Fluor 594, Texas Red|
|C16-25-550||25||33||546||562/576||2500.0||0.2500%||2500||45.6||Alexa Fluor 546, Alexa Fluor 555|
Conjugations (Ordering Abbreviation)
|Fluorophore Type||Excitation (nm)||Emission (nm)||Spectrally Similar Dyes|
|750||750||773||Alexa Fluor 750|
|680||673||707||Alexa Fluor 680, Cy5.5|
|650||646||662||Alexa Fluor 647, Cy5|
|633||638||658||Alexa Fluor 633|
|600||591||604||Alexa Fluor 594, Texas Red|
|550||555||570||Alexa Fluor 546, Alexa Fluor 555|
|488||494||518||FITC, Alexa Fluor 488|
These Plasmonic Fluorophores™ come in numerous conjugations:
Available in amine, biotin, carboxyl, methyl, neutravidin, streptavidin, nhs, maleimide, and azide forms. Methyl acts as a free acid form to be used as a non reactive negative control.
Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.
- Goat Anti-Human IgG
- Goat Anti-Mouse IgG
- Goat Anti-Rabitt IgG
- Goat Anti-Human IgA
- Goat Anti-Human IgM
This product is available in 2.5mg and 12.5mg quantities. Buffer is PBS.
All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.
Shelf Life/Storage Temperature
This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.
Unknown. Sterilization and Endoxtoin Free Options are available.
This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
Fluorescence (Nanopartz Flurophore I) for fluorescence measurements
- Covalent bonds insure specificity, stability, long shelf life
- Buffer Stability - stable from pH 4-9
- No sodium azide
- No BSA
- Polymer coating insures no aggregation in high salts, reduced nonspecific binding
- Well Characterized
- Customer can select buffer
- Customer can select gold nanoparticle type, size and/or SPR
- Loading of all ligands is optimized
- Customer can focus on research and not on Nanopartz™ expertise
- Quick turnaround
Microgold (Part #C13-1000-TMU-5) with MUTAB coating under brightfield microscope, cancer cell, 100x objective
How much thickness does your proprietary capping agent add?
It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.
How do I determine the concentration of the product?
Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solvent you add.
Why do you sell your rods as OD-mLs and what does this mean?
This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.
Can I resuspend in water?
Can I suspend in silicon oil?
Will the SPR wavelength shift with different solvents?
The SPR is a function of the index of refraction of the host solvent in which the nanoparticles are suspended. Nanorods are the most sensitive and are therefore a great tool for measuring the index of refraction of the host.
What is the shelf life if I never open the package?
Should I store in the refrigerator?
"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."
Stephan Link, PhD
Assistant Professor of Chemistry
Plasmon-enhanced brightness and photostability from single fluorescent proteins coupled to gold nanorods
plasmon-enhanced emission from intrinsically fluorescent proteins. ... Overall, our work indicates
that plasmonic particles improve both the brightness and photostability of FPs and extends the ...
Fluorescent nanothermometers provide controlled plasmonic-mediated intracellular hyperthermia
G, Quidant R. Mapping intracellular temperature using green fluorescent protein. ... Laura Martínez
Maestro. 1 Fluorescence Imaging Group, Departamento de Física de Materiales ...
Enhancing the optical excitation efficiency of a single self-assembled quantum dot with a plasmonic nanoantenna
structure ... The shallow depth of the emitters enables near-field coupling with plasmonic structures
placed on ... with a diameter of 90 nm as specified by the manufacturer (Nanopartz Inc.). ...
The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts
image (characterized morphological structure of the object), fluorescent image (was ... receptor
(EGFR) antibody C225 were fabricated using covalent conjugation by Nanopartz Inc. ...
[HTML] Super-resolution of fluorescence-free plasmonic nanoparticles using enhanced dark-field illumination based on wavelength-modulation
technique demonstrated the potential of super-resolution microscopy with fluorescence-free
material. ... and 80-nm SNP colloidal solutions were purchased from Nanopartz ™ (Salt Lake ...
Antibody-conjugated gold nanoparticles integrated in a fluorescence based biochip
Transmitted electrons are then focused on to a fluorescent screen to form an image. ... Citrate
stabilized and PEG conjugated gold nanoparticles were bought from Nanopartz, Salt ...
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
cells were observed by fluorescence microscopy 48 h after the treatment. ... by software (Image
Pro Plus, Media Cybernetics Inc.), taking into account the brightness of fluorescent cells of ...