Gold Nanoparticle Properties: Typical Oligo Loading

Important reference for determining the oligo density for gold nanoparticles

The following table shows the typical oligo (2-40 mers) loading for our spherical gold nanoparticles from 1.8nm to 1500nm. Please note that for sizes 1.8nm to 5nm we only ship 0.25mg unless the monovalent oligo loading is chosen.


Oligo Loading as a function of Gold Nanoparticles Size
Diameter (nm) NPS/ml Molarity (pM) Moles Surface Area (nm2) Oligo/gnp Optical Density (1cm) Oligos per order (nmoles)
1.8 4.25E+15 7.10E+06 7.09E-09 10 2 5 14.4
2.2 2.33E+15 3.90E+06 3.88E-09 15 3 5 11.8
3 9.19E+14 1.50E+06 1.53E-09 28 6 5 8.7
4 3.88E+14 6.50E+05 6.46E-10 50 10 5 6.5
5 1.99E+14 3.30E+05 3.31E-10 79 16 5 5.2
10 2.48E+14 4.10E+05 4.14E-10 314 63 50 26
15 7.35E+13 1.20E+05 1.23E-10 707 141 50 17.3
20 3.10E+13 5.20E+04 5.17E-11 1256 251 50 13
25 1.59E+13 2.60E+04 2.65E-11 1963 393 50 10.4
30 9.19E+12 1.50E+04 1.53E-11 2826 565 50 8.7
35 5.79E+12 9.60E+03 9.65E-12 3847 769 50 7.4
40 3.88E+12 6.50E+03 6.46E-12 5024 1005 50 6.5
45 2.72E+12 4.50E+03 4.54E-12 6359 1272 50 5.8
50 1.99E+12 3.30E+03 3.31E-12 7850 1570 50 5.2
55 1.49E+12 2.50E+03 2.49E-12 9499 1900 50 4.7
60 1.15E+12 1.90E+03 1.91E-12 11304 2261 50 4.3
65 9.04E+11 1.50E+03 1.51E-12 13267 2653 50 4
70 7.23E+11 1.20E+03 1.21E-12 15386 3077 50 3.7
75 5.88E+11 9.80E+02 9.80E-13 17663 3533 50 3.5
80 4.85E+11 8.10E+02 8.08E-13 20096 4019 50 3.2
85 4.04E+11 6.70E+02 6.73E-13 22687 4537 50 3.1
90 3.40E+11 5.70E+02 5.67E-13 25434 5087 50 2.9
95 2.89E+11 4.80E+02 4.82E-13 28339 5668 50 2.7
100 2.48E+11 4.10E+02 4.14E-13 31400 6280 50 2.6
150 7.35E+10 1.20E+02 1.23E-13 70650 14130 50 1.7
200 3.10E+10 5.20E+01 5.17E-14 125600 25120 50 1.3
500 1.99E+09 3.30E+00 3.31E-15 785000 157000 5 0.5
1000 2.48E+08 4.10E-01 4.14E-16 3140000 628000 5 0.3
1500 7.35E+07 1.20E-01 1.23E-16 7065000 1413000 5 0.2