All About EDC Chemistry

Definition, Guide, Steps, Instruction

To conduct EDC chemistry, which stands for 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide chemistry, several steps are typically involved. EDC/NHS chemistry is commonly used for bioconjugation processes due to its ability to target functional groups like amines and carboxylic acids (Coffman et al., 2018). This method involves an activation step where a carboxylic acid group is activated by EDC followed by NHS, and then a conjugation step where a nucleophile, such as an amine, reacts with the activated group to form an amide bond (Coffman et al., 2018).

Researchers have utilized EDC chemistry in various studies for different purposes. For instance, in drug delivery systems, EDC chemistry has been employed to conjugate molecules like estrone with oxidized-MWCNTs in multiple steps (Mehra & Jain, 2015). Additionally, in the field of glycomics, EDC chemistry has been used for linkage-specific modification of sialic acid residues (Dong et al., 2018). Moreover, in cancer therapy, EDC/NHS chemistry has been utilized to functionalize gold nanorods for targeted therapy (Choi et al., 2012).

Furthermore, EDC chemistry has been applied in the development of biosensors, such as in the detection of IL-6 biomarkers (Khan & Song, 2021). It has also been used in the synthesis of fluorescent copolymers for SARS-CoV-2 detection (Celiker et al., 2022). Additionally, EDC chemistry has been crucial in the fabrication of magnetic resonance sensing probes for enhanced detection limits (Wu et al., 2019).

Moreover, EDC chemistry has been instrumental in the conjugation of various biomolecules for different applications. For instance, it has been used in the conjugation of aptamers for cancer treatment (Odeh et al., 2019), in the attachment of peptides to nanofibers for drug delivery to melanoma tumors (Yang et al., 2018), and in the conjugation of antibodies to nanoparticles for specific cell targeting (Xu et al., 2014).

In summary, EDC chemistry plays a vital role in bioconjugation processes across various fields such as drug delivery, cancer therapy, biosensing, and nanotechnology. Its versatility and effectiveness in forming stable amide bonds make it a valuable tool for researchers in developing novel bioconjugates for a wide range of applications.

Go here for Nanopartz Carboxyl Functionalized Gold Nanoparticles for EDC Chemistry




Celiker, T., Ghorbanizamani, F., Moulahoum, H., Çelik, E., Tok, K., Zihnioğlu, F., … & Yagci, Y. (2022). Fluorescent bioassay for sars-cov-2 detection using polypyrene-g-poly(ε-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations. Microchimica Acta, 189(5).

Choi, J., Yang, J., Bang, D., Park, J., Suh, J., Huh, Y., … & Haam, S. (2012). Targetable gold nanorods for epithelial cancer therapy guided by near‐ir absorption imaging. Small, 8(5), 746-753.

Coffman, J., Metz, S., Brackbill, A., Paul, M., Miley, M., DeSimone, J., … & Tian, S. (2018). Optimization of surface display of denv2 e protein on a nanoparticle to induce virus specific neutralizing antibody responses. Bioconjugate Chemistry, 29(5), 1544-1552.

Dong, X., Huang, Y., Cho, B., Zhong, J., Gautam, S., Peng, W., … & Mechref, Y. (2018). Advances in mass spectrometry‐based glycomics. Electrophoresis, 39(24), 3063-3081.

Khan, N. and Song, E. (2021). Detection of an il-6 biomarker using a gfet platform developed with a facile organic solvent-free aptamer immobilization approach. Sensors, 21(4), 1335.

Mehra, N. and Jain, N. (2015). One platform comparison of estrone and folic acid anchored surface engineered mwcnts for doxorubicin delivery. Molecular Pharmaceutics, 12(2), 630-643.

Odeh, F., Nsairat, H., Alshaer, W., Ismail, M., Esawi, E., Qaqish, B., … & Ismail, S. (2019). Aptamers chemistry: chemical modifications and conjugation strategies. Molecules, 25(1), 3.

Wu, L., Xianyu, Y., Wang, Z., Dong, Y., & Hu, X. (2019). Amplified magnetic resonance sensing via enzyme-mediated click chemistry and magnetic separation. Analytical Chemistry, 91(24), 15555-15562.

Xu, Y., Baiu, D., Sherwood, J., McElreath, M., Qin, Y., Lackey, K., … & Bao, Y. (2014). Linker-free conjugation and specific cell targeting of antibody functionalized iron-oxide nanoparticles. Journal of Materials Chemistry B, 2(37), 6198.

Yang, M., Li, Y., Huai, Y., Wang, C., Yi, W., & Mao, C. (2018). Evolutionary selection of personalized melanoma cell/tissue dual-homing peptides for guiding bionanofibers to malignant tumors. Chemical Communications, 54(13), 1631-1634.