Photothermal imaging of passively targeted Nanopartz Ntracker nanorods to MDA-MB-435 tumors 72 hours after IV administration. (Nanopartz/MIT collaboration results)
Photothermal imaging of passively targeted Nanopartz Ntracker nanorods to soft tissue sarcoma tumors in canines 72 hours after IV administration. 10W 808nm laser was used for heating. (Nanopartz Clinical Trials)
Image of Nanopartz GNR labeled cell subsequently labeled with the live cell dye Calcein-AM, shown in red. From Motamedi 2011 BOE (2)5. NCBI Copyright Approval.
Two Photon Image of Nanopartz GNR labeled cells using an incident power of 0.7mW overlayed with corresponding transmitted light micrograph. From Motmaedi 2011 BOE(2)5. NCBI Copyright Approved.
Two photon image using low incident power of 1mW following intravenous injection of Nanopartz gold nanorods in Hamster Model showing blood vessels in the tissue. From Motamedi 2011 BOE (2) 5. NCBI Copyright Approved.
White light microscopy images of control tumor and GNS and Nanopartz Ntracker GNR injected mice. (Pink: cytoplasm stained with eosin, Dark Blue: nuclei stained with hematoxylin). From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
TPIP images of Tumor model (Green: cytoplasm stained with eosin, Yellow: GNSs and Nanopartz Ntracker gold nanorods). From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
TPIP images of Nanopartz gold nanorod injected mice with nuclei staning (YOYO-1 iodide). Two photon IP image with FOV of 720x490um measured at the distance of approximately 100um inside the tumor edge. (Green: nuclei, Yellow: Nanopartz Ntracker gold nanorods). From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
Two Photon IP images of Nanopartz gold nanorod injected mice with blood vessel staining (CD31 immunofluorescence staining). TPIP image with FOV of 790x520um. Red: blood vessels, Yellow: Nanopartz Ntracker Gold Nanorods. From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
Two Photon IP images of liver of Nanopartz Ntracker gold nanorod injected mice. From Park, Lasers Surg Med. 2010 September 42(7) 630. NCBI Copyright Approved.
In vivo imaging of single gold nanorods in mice ear blood vessels. Overlay of transmission image (light blue) and a single frame TPL image. Two single nanorods (red spots) are superimposed by a linescan (white). From Wei Nanomedicine (Lond). 2007 February 2(1) 125-132. NCBI Copyright Approval.
B Mode and photoacoustic images of subcutaneous tumor before and 2 minutes after a 200uL bolus of Nanopartz gold nanorods. Photoacoustic contrast in enhanced by gold nanorods in vasculature. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Ex vivo 3D photoacoustic images taken at 750nm of the excised spleen of a mouse 1 day after a tail vein injection of 200uL of Nanopartz gold nanorods and a control mouse spleen. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Ex vivo 3D photoacoustic images taken at 750nm of the excised liver of a mouse 1 day after a tail vein injection of 200uL of Nanopartz gold nanorods and a control mouse liver. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Ex vivo 3D photoacoustic images taken at 970nm of the excised liver of a mouse 1 day after a tail vein injection of 200uL of Nanopartz gold nanorods and a control mouse liver. From Visualsonics appliation note: Imaging of Nanoparticle Based Contrast Agents with Vevo LAZR Photoacoustic Imaging System. www.visualsonics.com
Gold nanorod contrast enhanced Optical Coherence Tomography images of a human breast carcinoma tissue sample. The sturcutral OCT image is presented in red, and the SOCT computed GNR density is presented in green.
Nanopartz™in vivo Gold Nanoparticles
Nanopartz™ gold nanoparticles for in vivo application include both the Ntracker™ and Ntherapy™ family of nanorods, along with customer defined oligo and antibody conjugated nanrods for in vivo applications. The Ntracker™ non reactive methyl terminated nanorods are coated in a proprietary dense layer of hydrophilic polymers that shield the gold surface and give the particles ultra-long circulation times. Ntherapy™ nanorods come with similar hydrophilic polymers, but with terminal amine and carboxyl active groups, along with a number of custom popular in vivo targeting ligands. Ntherapy™ also provides an opportunity for the customer to define their own targeting ligands utilizing Nanopartz™ nanorod and polymer technologies. In this family, Nanopartz™ has the ability to covalently conjugate to specific antibody locations, thereby for example, leaving necessary primary amines exposed for optimum activity. The combination of the highly monodisperse Nanopartz™ gold nanorods with the proprietary Nanopartz™ polymers increase circulation times 50% longer than other commercial polymers thereby significantly improving targeting. As opposed to other commercially available nanoparticles such as quantum dots, Nanopartz™ in vivo line of nanorods are completely non-toxic. Whereas Ntracker™ takes advantage of the Enhanced Permeability and Retention effect (EPR) for passive in vivo targeting, Ntherapy™ utilizes popular ligands for active targeting. This product line comes in a diameter of 10nm with SPRs matching all of the popular Near IR CW laser wavelengths; 780, 808, 850, 980, and 1064nm. Ntherapy™ offers options for oligo and customer detailed conjugations. Every batch is radiation sterilized in PBS, and comes with instructions for in vivomouse iv injection. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Every product is in stock (99%) and is shipped next day for many ligands. This product comes in two concentrations, regular 1.75mg/mL, and highly concentrated 10mg/mL.
Nanopartz™ In Vivo Plasmonic Fluorophores™ are fluorophore decorated gold nanorods manufactured using multiple patent pending and proprietary methods that result in fluorophores that exhibit much higher intensities than conventional fluorophores, no photobleaching even under the highest laser intensities, with long circulation times in vivo. Peak fluorescence range from 780 to 1064nm utilizing the same nanorods and in vivo polymer used in our Ntracker and Ntherapy products.
|Part #||Diameter (nm)||Peak SPR Wavelength (nm)||Optical Density (1cm)||NPS/ml||Wt. conc (mg/ml)||Wt. %||PPM||Molarity (pM)||Molar Ext. (M-1cm-1)||Absorption Molar Ext. (M-1cm-1)||Scattering Molar Ext. (M-1cm-1)||Size Dispersity %PDI||Notes|
|Size accuracy +/- 0.1 nm|
|Capping agent is covalently bonded carboxyl|
|Size accuracy +/- 2 nm|
|Peak SPR does not determine size for d<20 nm|
|Vol is 1mL|
|%PDI = Std Dev/Size|
|See Tech Note TN801 for definition of terms and method of analysis|
|All specs typical. May vary batch to batch. Exact values are measured for each batch|
|OD >=50 for all sizes|
|Shape monodispersity (% spheres) > 99.9%|
|Solution is 18MEG DI water|
|Residual Chemicals < 0.1%|
|Part #||Diameter (nm)||Length (nm)||Peak SPR Wave (nm)||Aspect Ratio||OD SPR (AU)||Peak LSPR Wave (nm)||OD LSPR (AU)||Nanorod Vol (nm3)||Nanorods /mL||Wt. conc (mg/ml)||Wt. %||PPM||Molarity (nM)||SPR Molar Ext. (M-1cm-1)||LSPR Molar Ext. (M-1cm-1)||Peak SPR accuracy (nm)||SPR Linewidth 80% (nm)|
|Part #||Diam (nm)||Long Size (nm)||Peak SPR Wave (nm)||Ex/Em (nm)||Wt. conc (mg/ml) (10-3)||Wt. %||PPM||Molarity (pM)||Spectrally Similar Dyes|
|C16-10-780||10||38||780||754/776||1750.0||0.1750%||1750||66.4||Alexa Fluor 750|
|C16-25-750||25||86||750||754/776||2500.0||0.2500%||2500||7.0||Alexa Fluor 750|
|C16-25-680||25||68||680||692/712||2500.0||0.2500%||2500||9.9||Alexa Fluor 680, Cy5.5|
|C16-25-650||25||60||650||652/672||2500.0||0.2500%||2500||12.0||Alexa Fluor 647, Cy5|
|C16-25-633||25||56||633||638/658||2500.0||0.2500%||2500||13.6||Alexa Fluor 633|
|C16-25-600||25||46||594||593/618||2500.0||0.2500%||2500||19.9||Alexa Fluor 594, Texas Red|
|C16-25-550||25||33||546||562/576||2500.0||0.2500%||2500||45.6||Alexa Fluor 546, Alexa Fluor 555|
Functionalized gold nanoparticle with Nanopartz™ Ntherapy covalently bonded polymer. Thickness of polymer is less than the size variablity of the gold nanoparticle.
Conjugations (Ordering Abbreviation)
|Fluorophore Type||Excitation (nm)||Emission (nm)||Spectrally Similar Dyes|
|750||750||773||Alexa Fluor 750|
|680||673||707||Alexa Fluor 680, Cy5.5|
|650||646||662||Alexa Fluor 647, Cy5|
|633||638||658||Alexa Fluor 633|
|600||591||604||Alexa Fluor 594, Texas Red|
|550||555||570||Alexa Fluor 546, Alexa Fluor 555|
|488||494||518||FITC, Alexa Fluor 488|
These polymer coated gold nanorods are shipped in PBS with no measurable residual chemicals. This product is radiation sterilized and is ready for injection.
Popular CW laser wavelengths are available (780, 808, 850, 980, and 1064nm). Other sizes are special order. Please contact us.
This product is available in 1mL and larger but in two different concentrations. The lower concentration is suited for mice, while the higher concentration is suitable for companion animals. For mice, the 1mL injection is suited for 5 injections. For orders larger than 1L, or for orders amounting over 10L per annum, please contact sales for quantity pricing.
Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.
This product comes coated with a proprietary polymer to increase circulation times. Ntracker™ contains a non-reactive methyl termination. Ntherapy™ contains many choices for reactive terminations. Many popular in vivo conjugations are available.
Unfortunately there are no kits available for this product at this time.
Shelf Life/Storage Temperature
This product is guaranteed for one year and may be stored at room temperature.
These products are non cytotoxic.
This product is sterilized.
This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis that includes the following information. We use NIST traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
Ntracker™ is based on extensive research in laboratory, preclinical, toxicology, and more recently clinical trials. The technology is based on patented, patent pending, and proprietary methods. Nanopartz™ has the capacity for up to 1kg of Ntracker™product per day in its audited ISO 9000 facility. Ntherapy™ offers the same technology as Ntracker™ with the added advantage of popular in vivo conjugations and customer specified oligo and antibody choices.
This product has been extensively tested in mice and companion animals.
Photoacoustic is provided by our partner:
VisualSonics is the world leader in real-time, in vivo, high-resolution, micro-imaging systems, providing modalities specifically designed for preclinical research. VisualSonics has commercialized Vevo LAZR Technology, a state-of-the-art photoacoustic imaging system with inherent coregistration and capabilities for imaging gold nanorods in vivo. "Listen to the Light" - Photoacoustic imaging with the new Vevo LAZR System.
- Covalent bonds insure specificity, stability, long shelf life
- Buffer Stability - stable from pH 4-9
- No sodium azide
- No BSA
- Polymer coating insures no aggregation in high salts, reduced nonspecific binding
- Well Characterized
- Customer can select buffer
- Customer can select gold nanoparticle type, size and/or SPR
- Loading of all ligands is optimized
- Customer can focus on research and not on Nanopartz™ expertise
- Quick turnaround
"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."
Stephan Link, PhD
Assistant Professor of Chemistry
[HTML] Infrared-Transparent Gold Nanoparticles Converted by Tumors to Infrared Absorbers Cure Tumors in Mice by Photothermal Therapy
polycarbonate membrane (Millipore, Billerica, MA) . One side was loaded with either
15 nm AuNPs or 10×45 nm Au nanorods (Nanopartz, Loveland, CO) in PBS. ...
vivo tumor targeting and spectroscopic detection with surface-enhanced Raman ... Gold nanorod
assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell ...
Noninvasive< i> in vivo</i> spectroscopic nanorod-contrast photoacoustic mapping of sentinel lymph nodes
in a rat ... top view and a bottom view of the dissected SLN, in which the nanorod-accumulated parts ...
Before the injection of the gold nanorods, a PA image was acquired as a control to ...
In vivo imaging and treatment of solid tumor using integrated photoacoustic imaging and high intensity focused ultrasound system
experiments, the peak optical absorption wavelength of gold nanorods (30-pm-700 ... Figure shows
that strong PA signals are generated from the nanorod solution, whereas the PA ...
method has been developed for their nanorod products. ... However, the limitation on commercialising
gold nanorods for in-vivo and in-vitro applications has been toxicity. ...
be used to inhibit expression of target genes in vitro and in vivo. ... other than phosphodiester
linkages, provided the appropriate zeta potential of the gold nanorod/ RNA polynucleotide ...
Resolving rotational motions of nano-objects in engineered environments and live cells with goldnanorods and differential interference contrast microscopy
(CTAB)-capped (25 × 73 nm, 1.3 × 10 11 particles/mL, Nanopartz) gold nanorods. ... Briefly, excessive
surfactant was first removed from 1.0 mL of gold nanorod solution by centrifugation at 3000g ...
Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages
Biodistribution of gold nanoparticles in ovarian tumor-bearing mice. The in vivo fate of the
nanoparticles and their transport across biological barriers by a particular administration ...
(NRs) were purchased from Nanopartz with a peak plasmon resonance at 800 nm and ... LPs were
intravenously injected in vivo to ensure control LPs and LyP1LPs exhibited similar ...
aspect ratio ... help with the photothermal imaging setup, Christian Schoen from Nanopartz for
supplying the gold nanospheres and ... 2005) Quantum dots for live cells, in vivo imaging, and ...
introduced the concept of a “soft” type self-assembled gold nanorod contrast agent for efficient
NIR ... Further in vitro and in vivo works are warranted to realize the full potential of the ...
in vivo trials ... the amplitude of the acoustic tran- sients generated at the surface of a methyl
terminated polymer conjugated gold nanorod colloidal solution (Nanopartz, Inc.) with ...
The iron nanoparticles run about $200 per 10 g, ad gold nanoparticles cost $190 ... "Noninvasive
laser-induced photoacoustic tomography for structural and functional in vivo imaging of ...
Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence
to the gold colloid suspension resulting in a silica particle covered with gold colloid. For in vivo
applications, a layer of 5,000 MW poly-(ethylene glycol) was added to the exterior shell ...
Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres
from Nanopartz (Salt Lake City, UT, USA) and sterilized by filtra- tion upon receiving. ... PEG-modified
gold nanorod with a stealth character for in vivo applications. J. Control. ...
Salt Lake City, UT) were tuned to the laser ... clearly display gold nanorods in the cross-section of
the cell (B and C). Transmission electron micrographs of gold nanorod-labeled 1321N1 ...
Dual-Modality Single Particle Orientation and Rotational Tracking of Intracellular Transport of Nanocargos
which ... CTAB)-capped (25 nm × 73 nm, 1.3 × 10 11 particles/mL, Nanopartz) gold nanorods. ... Briefly,
excessive surfactant was first removed from 1.0 mL of the gold nanorod solution by ...
which has been shown to provide in vitro and in vivo stability (Johnsson ... order to obtain a
comparative indication of the effect of shape, the deposition of a gold nanorod was tested ...
F1-ATPase mol- ecules observed to rotate via single-molecule microscopy with gold nanorod
probes ... Klionsky, DJ, Brusilow, WS, and Simoni, R. D. (1984) In vivo evidence for the role of ...
shaped particles have a higher probability of cell internalization than rod-shaped particles. When
nanopar- ticles are used in vivo, one of the most important issues is to avoid ...
Synthesis, Characterization, and Optical Response of Gold Nanoshells Used to Trigger Release from Liposomes
accumulate NIR light (Hirsch et al., 2003), except that HGNs have a hollow core, which allows
easier synthesis, (Prevo et al., 2008) smaller overall dimensions ( [Prevo et al., 2008 ...
time surface temperature assessment via infrared thermography FLIR Thermacam to facilitate
comparison of model predictions against in vivo experimental recordings. ...
Imaging and Absolute Extinction Cross-Section Measurements of Nanorods and Nanowires through Polarization Modulation Microscopy†
that is almost perfectly polarized, ie, σ ≈ 0.(12) Images of a gold nanorod recorded at 800 nm
for two different angles of the λ/2-waveplate are shown in Figures 2, panels a and b ...
nanoparticles show promise for in vivo identification of ... The nanorods purchased from Nanopartz,
Inc ... Lyvers, DPet al., “Gold nanorod arrays as plasmonic cavity resonators,” ACS Nano ...
J.-X. Cheng, “Gold nanorod-mediated photothermolysis induces apoptosis of macrophages via ...
Petri dishes and grown for 1-2 days before incubation with colloidal gold nanoparticles of ...
Enhanced visualization of biodegradable polymeric vascular scaffolds by incorporation of gold, silver and magnetite nanoparticles
USA ... Hence, in a potential in vivo setting the incorporated nanopar- ticles should be firmly ... P(4HB)
minitube samples doped with different nanoparticles: 0.1% (w) gold nanoparticle (a ...
Photothermal multispectral image cytometry for quantitative histology of nanoparticles and micrometastasis in intact, stained and selectively burned tissues
single cell level both in vivo and in ... Gold nanorods (GNRs) with axial size 30 nm, diameter 10
nm and peak absorption at 740 nm were purchased from Nanopartz (Loveland, CO). ...
[PDF] Biocompatible Microparticles for Optically Driven On-demand Controlled Drug Release of Methotrexate
for the main component of the gold-NIR, light-to-heat energy ... The gold nanorod solution will be
pipetted into the cuvette, and the thermistor will be placed under the aluminum bottom. ...
spherical gold nanoparticles ..... 61 5.3.2. Enhanced nonlinear absorption using gold nanorods ..... ...
nanoshells (NS, 50±7 nm diameter, 2.7±0.8 nm thickness, optical extinction peak
at 665 nm under the same conditions) following established ...
(part #: 30-25- 730) AuNRs were obtained from Nanopartz. 2.5 um long gold nanowires were
synthesized and obtained from Prof. Zubarev's group at Rice University. Microscope ...
and in vivo detection of ... Ultrasharp PT resonances made it possible to easily identify each nanorod
in a mixture of six nanorods that ... Figure 2: Nonlinear spectra of gold nanorods. ...
Superresolution Fluorescence Imaging of Mitochondrial Nucleoids Reveals Their Spatial Range, Limits, and Membrane Interaction
min. After being rinsed, the glass was coated with biotin (1 mg/ml)-BSA-PBS (Sigma Chemical
A8549) followed by streptavidin–PBS (Sigma Chemical S462) (1 mg/ml solution). ...
embodiments;  FIGS. 8A-8E illustrate nanorod-directed tumor heating and
fibrinogen deposition, in another set of embodi- ments;  FIGS. ...
technique by the use of gold nanoparticles as new chromophores. Page 9. ... performed on ex
vivo pig eyes; the healing process was then studied in vivo in rabbits. The ...
(52) US CI 600/411; 606/41; 600/439; 600/407 (57) ABSTRACT The present invention provides
methods, devices, and sys- tems for in vivo treatment of cell proliferative disorders. ...
permission from http://www.nanopartz.com/. Download figure to PowerPoint. ... Reproduced with
permission,27 copyright 2007 AIP. (f) Gold helices made by two-photon lithography. ...
ENHANCED RAMAN SPECTROSCOPY (SERS) USING METALLIC NANOCRYSTALS ... Figure
5.5 Trapping and transport of five 250 nm gold nanoparticles. ...