Photomicrograph

Nanopartz™ Gold Nanoparticles for In Vitro Dark Field Imaging

Nanopartz™ Gold Nanoparticles for in vitro Dark Field Imaging utilize the complete line of nanospheres and nanorods for dark field microscopy from 500nm to the near IR. These conjugated particles exhibit extinctions many orders of magnitude greater than fluorophores. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. This product comes in two concentration, 2.5mg/mL and 12.5mg/mL. The highly concentrated gold nanoparticles come in a range of conjugations.

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Conjugated Spherical Gold Nanoparticles for In-Vitro
Conjugated Spherical Gold Nanoparticles for In-Vitro
Part Number: C11
Options: (DIAMETER)-(FUNCTIONALIZATION)-(MONO)-(BUFFER)-(OD-mLs)-(VOL)-CS-EP-NC
Example Part Number: 30nm diameter spherical gold nanoparticle, carboxyl polymer, 18MEG DD DI Water, 50 OD (2.5mg), in 1mL, Endotoxin Purified, and 0.1mL Negative Control
C11-30-TC-DIH-50-1-EP-0.1NC
QUICK SPECS
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Part #
Diameter (nm)
Peak SPR Wavelength (nm)
NPS/ml
Molarity (pM)
Moles
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Size Accuracy (+/- nm)
C11-1.8
1.8
n/a
4.25E+16
7.10E+07
7.09E-08
7.05E+05
7.05E+05
0.00E+00
<35%
0.1
C11-2.2
2.2
n/a
2.33E+16
3.90E+07
3.88E-08
1.29E+06
1.29E+06
0.00E+00
<25%
0.1
C11-3
3
n/a
9.19E+15
1.50E+07
1.53E-08
3.26E+06
3.26E+06
0.00E+00
<20%
0.1
C11-4
4
n/a
3.88E+15
6.50E+06
6.46E-09
7.74E+06
7.74E+06
0.00E+00
<20%
1
C11-5
5
512
1.99E+15
3.30E+06
3.31E-09
1.51E+07
1.51E+07
0.00E+00
<20%
2
C11-10
10
516
2.48E+14
4.10E+05
4.14E-10
1.21E+08
1.21E+08
0.00E+00
<15%
2
C11-15
15
518
7.35E+13
1.20E+05
1.23E-10
4.08E+08
4.08E+08
0.00E+00
<15%
2
C11-20
20
520
3.10E+13
5.20E+04
5.17E-11
9.67E+08
9.67E+08
0.00E+00
<10%
2
C11-25
25
521
1.59E+13
2.60E+04
2.65E-11
1.89E+09
1.89E+09
0.00E+00
<10%
2
C11-30
30
523
9.19E+12
1.50E+04
1.53E-11
3.26E+09
3.26E+09
0.00E+00
<6%
2
C11-35
35
526
5.79E+12
9.60E+03
9.65E-12
5.18E+09
5.18E+09
0.00E+00
<6%
2
C11-40
40
527
3.88E+12
6.50E+03
6.46E-12
7.74E+09
7.72E+09
2.24E+07
<4%
2
C11-45
45
529
2.72E+12
4.50E+03
4.54E-12
1.10E+10
1.08E+10
2.03E+08
<4%
2
C11-50
50
531
1.99E+12
3.30E+03
3.31E-12
1.51E+10
1.45E+10
5.88E+08
<4%
2
C11-55
55
533
1.49E+12
2.50E+03
2.49E-12
2.01E+10
1.88E+10
1.30E+09
<4%
2
C11-60
60
536
1.15E+12
1.90E+03
1.91E-12
2.61E+10
2.36E+10
2.48E+09
<4%
2
C11-65
65
539
9.04E+11
1.50E+03
1.51E-12
3.32E+10
2.89E+10
4.33E+09
<4%
2
C11-70
70
542
7.23E+11
1.20E+03
1.21E-12
4.15E+10
3.44E+10
7.09E+09
<4%
2
C11-75
75
545
5.88E+11
9.80E+02
9.80E-13
5.10E+10
4.00E+10
1.10E+10
<4%
2
C11-80
80
549
4.85E+11
8.10E+02
8.08E-13
6.19E+10
4.54E+10
1.65E+10
<4%
2
C11-85
85
553
4.04E+11
6.70E+02
6.73E-13
7.42E+10
5.03E+10
2.39E+10
<4%
2
C11-90
90
558
3.40E+11
5.70E+02
5.67E-13
8.81E+10
5.44E+10
3.37E+10
<4%
2
C11-95
95
563
2.89E+11
4.80E+02
4.82E-13
1.04E+11
5.72E+10
4.65E+10
<4%
2
C11-100
100
569
2.48E+11
4.10E+02
4.14E-13
1.21E+11
5.82E+10
6.27E+10
<4%
2
C11-150
150
612
7.35E+10
1.20E+02
1.23E-13
4.08E+11
0.00E+00
4.08E+11
<4%
10
C11-200
200
n/a
3.10E+10
5.20E+01
5.17E-14
9.67E+11
0.00E+00
9.67E+11
<4%
10
C11-500
500
n/a
1.99E+09
3.30E+00
3.31E-15
1.51E+13
0.00E+00
1.51E+13
<4%
50
C11-1000
1000
n/a
2.48E+08
4.10E-01
4.14E-16
1.21E+14
0.00E+00
1.21E+14
<4%
100
C11-1500
1500
n/a
7.35E+07
1.20E-01
1.23E-16
4.08E+14
0.00E+00
4.08E+14
<4%
100

UV-VIS

Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$440.00
PEG Spherical Gold Nanoparticles
PEG Spherical Gold Nanoparticles
Part Number: CP11-(DIAMETER)-(FUNCTIONALIZATION)-(PEG MW)-(Buffer)-(OD-mLs)-(VOL)-(Sterilization)-(Endoxtoxin). Example part number for a 30nm diameter sphere, with a 5k Methyl PEG conjugation, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CP11-30-PM-5K-DIH-50-1

UV-VIS

OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$400.00
PEG Functionalized Silica Coated Spherical Gold Nanoparticles
PEG Functionalized Silica Coated Spherical Gold Nanoparticles
Part Number Format: CP611-(DIAMETER)-(FUNCTIONALIZATION)-(PEG MW)-(FLUOROPHORE)-(Buffer)-(OD)-(VOL)-(Sterilization)-(Endoxtoxin)-(NONREACTIVE CONTROL). Example part number for a 30nm diameter sphere, with a 5k Methyl PEG conjugation, 5nm silica in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CP611-30-PM-5K-5-DIH-50-1

UV-VIS

Silica Thickness (nm)
Additional Fluorophore tag from 400nm to 800nm
Price$540.00
CP612 PEG Functionalized Silica Coated Gold Nanorods
CP612 PEG Functionalized Silica Coated Gold Nanorods
Part Number Format: CP612-(DIAM/SPR)- (SILICA THICKNESS)-(FLUOROPHORE)-(FUNCTIONALIZATION)-(PEG MW)-(Buffer)-(OD)-(VOL)-(Sterilization)-(Endoxtoxin)-(NONREACTIVE CONTROL). Example part number for a 10nm diameter rod with SPR=808nm, with a 5k Methyl PEG conjugation, 5nm silica in water, 550 fluorophore, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CP612-10/808-5-550-PM-5K-DIH-50-1
Silica Thickness (nm)
Additional Fluorophore tag from 400nm to 800nm
Price$540.00
PEG Functionalized Silica Coated Microgold
PEG Functionalized Silica Coated Microgold
Part Number Format: CP613-(DIAM/LENGTH)-(FUNCTIONALIZATION)-(PEG MW)-(FLUOROPHORE)-(Buffer)-(Wt.)-(VOL)-(Sterilization)-(Endoxtoxin)-(NONREACTIVE CONTROL). Example part number for a 75nm diameter rod with length=500nm, with a 5k Methyl PEG conjugation, 5nm silica in water, at 0.25mg, 1mL would be: CP613-75/500-PM-5K-5-DIH-0.25-1
Silica Thickness (nm)
Additional Fluorophore tag from 400nm to 800nm
Price$480.00
PEG Functionalized Silica Coated Gold Nanowires
PEG Functionalized Silica Coated Gold Nanowires
Part Number Format: CP614-(DIAM/LENGTH)-(FUNCTIONALIZATION)-(PEG MW)-(FLUOROPHORE)-(Buffer)-(Wt.)-(VOL)-(Sterilization)-(Endoxtoxin)-(NONREACTIVE CONTROL). Example part number for a 75nm diameter rod with length=10000nm, with a 5k Methyl PEG conjugation, 5nm silica in water, at 0.25mg, 1mL would be: CP614-75/10000-PM-5K-5-DIH-0.25-1
Silica Thickness (nm)
Additional Fluorophore tag from 400nm to 800nm
Price$480.00
Conjugated Gold Nanorods for In-Vitro
Conjugated Gold Nanorods for In-Vitro
Part Number: C12
Options: (DIAM-SPR)-(FUNCTIONALIZATION)-(BUFFER)-(OD-mLs)-(VOL)-CS-EP-NC
Example Part Number: 10x45nm diameter gold nanorod with SPR=850nm, carboxyl polymer, 18MEG DD DI Water, 50 OD (2.5mg), in 1mL, Endotoxin Purified, and 0.1mL Negative Control
C12-10-850-TC-DIH-50-1-EP-0.1NC

QUICK SPECS

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Part #
Diameter (nm)
Length (nm)
Peak SPR Wave (nm)
Aspect Ratio
OD SPR (AU)
Peak LSPR Wave (nm)
OD LSPR (AU)
Nanorods /mL
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (nM)
Picomoles
SPR Molar Ext. (M-1cm-1)
LSPR Molar Ext. (M-1cm-1)

Peak SPR

(nm)
SPR Linewidth 80% (nm)
C12-5-808
5
21
808
4.2
50
510
10
2.39E+14
1750
0.18%
1750
398.72
3.99E+02
1.25E+08
2.51E+07
794-829
100
C12-5-780
5
19
780
3.8
50
510
10
2.67E+14
1750
0.18%
1750
444.72
4.45E+02
1.12E+08
2.25E+07
765-794
100
C12-5-700
5
15
700
3
50
510
10
3.47E+14
1750
0.18%
1750
578.14
5.78E+02
8.65E+07
1.73E+07
675-725
100
C12-10-2100
10
175
2100
17.5
50
510
10
6.73E+12
1750
0.18%
1750
11.21
1.12E+01
4.46E+09
8.92E+08
1900-2300
300
C12-10-1400
10
102
1400
10.2
50
510
10
1.17E+13
1750
0.18%
1750
19.47
1.95E+01
2.57E+09
5.14E+08
1300-1500
150
C12-10-1200
10
81
1200
8.1
50
510
10
1.48E+13
1750
0.18%
1750
24.67
2.47E+01
2.03E+09
4.05E+08
1132-1300
150
C12-10-1064
10
67
1064
6.7
50
510
10
1.81E+13
1750
0.18%
1750
30.13
3.01E+01
1.66E+09
3.32E+08
1022-1132
150
C12-10-980
10
59
980
5.9
50
510
10
2.09E+13
1750
0.18%
1750
34.91
3.49E+01
1.43E+09
2.86E+08
965-1022
150
C12-10-950
10
55
950
5.5
50
510
10
2.22E+13
1750
0.18%
1750
37
3.70E+01
1.35E+09
2.70E+08
925-965
150
C12-10-900
10
50
900
5
50
510
10
2.47E+13
1750
0.18%
1750
41.11
4.11E+01
1.22E+09
2.43E+08
875-925
150
C12-10-850
10
45
850
4.5
50
510
10
2.78E+13
1750
0.18%
1750
46.25
4.63E+01
1.08E+09
2.16E+08
829-875
100
C12-10-808
10
41
808
4.1
50
510
10
3.10E+13
1750
0.18%
1750
51.68
5.17E+01
9.68E+08
1.94E+08
794-829
75
C12-10-780
10
38
780
3.8
50
510
10
3.36E+13
1750
0.18%
1750
56.06
5.61E+01
8.92E+08
1.78E+08
765-794
65
C12-10-750
10
35
750
3.5
50
510
10
3.70E+13
1750
0.18%
1750
61.67
6.17E+01
8.11E+08
1.62E+08
725-765
50
C12-10-700
10
29
700
2.9
50
510
10
4.44E+13
1750
0.18%
1750
74
7.40E+01
6.76E+08
1.35E+08
675-725
40
C12-10-650
10
24
650
2.4
50
510
10
5.55E+13
1750
0.18%
1750
92.5
9.25E+01
5.41E+08
1.08E+08
625-675
40
C12-10-600
10
19
600
1.9
50
510
10
7.40E+13
1750
0.18%
1750
123.34
1.23E+02
4.05E+08
8.11E+07
575-625
40
C12-25-1400
25
245
1400
9.8
50
514
15
1.12E+12
2500
0.25%
2500
1.86
1.86E+00
2.69E+10
8.06E+09
1232-1500
150
C12-25-1064
25
137
1064
5.5
50
514
15
2.05E+12
2500
0.25%
2500
3.42
3.42E+00
1.46E+10
4.38E+09
1022-1232
150
C12-25-980
25
119
980
4.8
50
514
15
2.39E+12
2500
0.25%
2500
3.98
3.98E+00
1.26E+10
3.77E+09
965-1022
150
C12-25-950
25
102
950
4.1
50
514
15
2.82E+12
2500
0.25%
2500
4.7
4.70E+00
1.06E+10
3.19E+09
925-965
150
C12-25-900
25
96
900
3.8
50
514
15
3.01E+12
2500
0.25%
2500
5.02
5.02E+00
9.95E+09
2.99E+09
875-925
150
C12-25-850
25
93
850
3.7
50
514
15
3.12E+12
2500
0.25%
2500
5.2
5.20E+00
9.61E+09
2.88E+09
829-875
150
C12-25-808
25
90
808
3.6
50
514
15
3.24E+12
2500
0.25%
2500
5.39
5.39E+00
9.27E+09
2.78E+09
794-829
150
C12-25-780
25
87
780
3.5
50
514
15
3.36E+12
2500
0.25%
2500
5.6
5.60E+00
8.93E+09
2.68E+09
765-794
150
C12-25-750
25
85
750
3.4
50
514
15
3.45E+12
2500
0.25%
2500
5.75
5.75E+00
8.70E+09
2.61E+09
725-765
65
C12-25-700
25
75
700
3
50
514
15
3.96E+12
2500
0.25%
2500
6.61
6.61E+00
7.57E+09
2.27E+09
675-725
60
C12-25-650
25
71
650
2.8
50
514
15
4.22E+12
2500
0.25%
2500
7.03
7.03E+00
7.11E+09
2.13E+09
625-675
60
C12-25-600
25
57
600
2.3
50
514
15
5.43E+12
2500
0.25%
2500
9.05
9.05E+00
5.52E+09
1.66E+09
575-625
60
C12-25-550
25
34
550
1.4
50
514
15
1.01E+13
2500
0.25%
2500
16.91
1.69E+01
2.96E+09
8.87E+08
525-575
60
C12-40-1064
40
208
850
5.2
50
520
20
5.30E+11
2500
0.25%
2500
0.88
8.84E-01
5.66E+10
2.26E+10
1022-1232
150
C12-40-980
40
180
850
4.5
50
520
20
6.19E+11
2500
0.25%
2500
1.03
1.03E+00
4.84E+10
1.94E+10
875-1022
150
C12-40-850
40
148
850
3.7
50
520
20
7.67E+11
2500
0.25%
2500
1.28
1.28E+00
3.91E+10
1.57E+10
829-875
150
C12-40-808
40
134
808
3.4
50
520
20
8.56E+11
2500
0.25%
2500
1.43
1.43E+00
3.51E+10
1.40E+10
794-829
150
C12-40-780
40
124
780
3.1
50
520
20
9.33E+11
2500
0.25%
2500
1.55
1.55E+00
3.22E+10
1.29E+10
765-794
150
C12-40-750
40
112
750
2.8
50
520
20
1.05E+12
2500
0.25%
2500
1.74
1.74E+00
2.87E+10
1.15E+10
725-765
150
C12-40-700
40
92
700
2.3
50
520
20
1.31E+12
2500
0.25%
2500
2.19
2.19E+00
2.29E+10
9.14E+09
675-725
60
C12-40-650
40
80
650
2
50
520
20
1.55E+12
2500
0.25%
2500
2.58
2.58E+00
1.94E+10
7.75E+09
625-675
60
C12-40-600
40
68
600
1.7
50
520
20
1.89E+12
2500
0.25%
2500
3.15
3.15E+00
1.59E+10
6.35E+09
575-625
60
C12-40-550
40
60
550
1.5
50
520
20
2.21E+12
2500
0.25%
2500
3.69
3.69E+00
1.36E+10
5.42E+09
525-575
60
C12-50-1064
50
245
1064
4.9
50
530
25
2.89E+11
2500
0.25%
2500
0.48
4.82E-01
1.04E+11
5.18E+10
980-1232
150
C12-50-808
50
145
808
2.9
50
530
25
5.15E+11
2500
0.25%
2500
0.86
8.58E-01
5.83E+10
2.91E+10
750-850
150
C12-50-700
50
110
700
2.2
50
530
25
7.08E+11
2500
0.25%
2500
1.18
1.18E+00
4.24E+10
2.12E+10
650-750
150
C12-50-600
50
100
600
2
50
530
25
7.93E+11
2500
0.25%
2500
1.32
1.32E+00
3.78E+10
1.89E+10
550-650
150
C12-70-750
70
120
750
1.7
50
530
25
3.49E+11
2500
0.25%
2500
0.58
5.81E-01
8.60E+10
4.30E+10
700-800
150
C12-70-650
70
105
650
1.5
50
530
25
4.13E+11
2500
0.25%
2500
0.69
6.88E-01
7.27E+10
3.63E+10
600-700
150
C12-70-550
70
91
550
1.3
50
530
25
4.98E+11
2500
0.25%
2500
0.83
8.30E-01
6.02E+10
3.01E+10
500-600
150
DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
DIH (18MEG DD DI Water)
PBS (Phosphate Buffer Saline pH=7)
MES (pH=4.7)
SOBO (Sodium Borate pH=8.5)
TRIS (pH=7.2)
DRY (Lyophilized, ignore volume option)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg for nanorods >=25nm, 1.75mg for nanorods 5nm and 10nm in diameter
Price$480.00
PEG Gold Nanorods
PEG Gold Nanorods
Part Number: CP12-(DIAM/SPR)-(FUNCTIONALIZATION)-(PEG MW)-(Buffer)-(OD-mLs)-(VOL)-(Sterilization)-(Endoxtoxin). Example part number for a 10nm diameter rod with an SPR=808nm, with a 5k Methyl PEG conjugation, in water, at 50 OD-mLs (2.5mg/mL, OD=50, 1mL) would be: CP12-10-808-PM-5K-DIH-50-1
DIAM-SPR: Diameter (5nm, 10nm, 25nm, 40nm, 50nm and 70nm option) SPR = Longitudinal Surface Plasmon Resonance (550nm to 2100nm)
OD-mLs = Optical Density x mLs
50 OD-mLs = 2.5mg Au
250 OD-mL = 12.5mg Au
Price$440.00
Functionalized Ramanprobes
Functionalized Ramanprobes
Part Number: C17
Options: (WAVELENGTH)-(RAMANLABEL)-(FUNCTIONALIZATION)-(BUFFER)-(OD-mLs)-(VOL)-CS-EP-NC
Example Part Number: 785nm, Label A, carboxyl polymer, 18MEG DD DI Water, 50 OD (2.5mg), in 1mL, Endotoxin Purified, and 0.1mL Negative Control
C17-785-A-TC-DIH-50-1-EP-0.1NC
Note: NHS and Maleimide conjugations come dry
PEG (PEG)
TA (Amine)
TB (Biotin)
TC (Carboxyl)
TH (Hydroxyl)
TM (Methyl)
TN (Neutravidin)
TG (GSH)
TS (Streptavidin)
TNHS (NHS)
TMAL (Maleimide)
TMU (Mutab)
TPA (Protein A)
TG (Protein G)
TSH (Thiol)
TGAL (Galactose)
THRP (Horseradish Peroxidase)
Price$480.00

 

 

Part# CD11 Spherical Gold Nanoparticles for Darkfield Imaging

 

 

Part # Diameter (nm) Peak SPR Wavelength (nm) Optical Density (1cm) NPS/ml Wt. conc (mg/ml) Wt. % PPM Molarity (pM) Molar Ext. (M-1cm-1) AEsorption Molar Ext. (M-1cm-1) Scattering Molar Ext. (M-1cm-1) Size Dispersity %PDI Notes
CD11-1.8 1.8 505-508 50 4.25E+16 2.5 0.25% 2500 7.10E+07 7.05E+05 7.05E+05 0.00E+00 <35% 1,3
CD11-2.2 2.2 505-508 50 2.33E+16 2.5 0.25% 2500 3.90E+07 1.29E+06 1.29E+06 0.00E+00 <25% 1,3
CD11-3 3 505-508 50 9.19E+15 2.5 0.25% 2500 1.50E+07 3.26E+06 3.26E+06 0.00E+00 <20% 1,3
CD11-4 4 505-508 50 3.88E+15 2.5 0.25% 2500 6.50E+06 7.74E+06 7.74E+06 0.00E+00 <20% 1,3
CD11-5 5 505-508 50 1.99E+15 2.5 0.25% 2500 3.30E+06 1.51E+07 1.51E+07 0.00E+00 <20% 2,3
CD11-10 10 514-520 50 2.48E+14 2.5 0.25% 2500 4.10E+05 1.21E+08 1.21E+08 0.00E+00 <15% 2,3
CD11-15 15 514-520 50 7.35E+13 2.5 0.25% 2500 1.20E+05 4.08E+08 4.08E+08 0.00E+00 <15% 2,3
CD11-20 20 514-520 50 3.10E+13 2.5 0.25% 2500 5.20E+04 9.67E+08 9.67E+08 0.00E+00 <10% 2,3
CD11-25 25 514-520 50 1.59E+13 2.5 0.25% 2500 2.60E+04 1.89E+09 1.89E+09 0.00E+00 <10% 2
CD11-30 30 523 50 9.19E+12 2.5 0.25% 2500 1.50E+04 3.26E+09 3.26E+09 0.00E+00 <6% 2
CD11-35 35 526 50 5.79E+12 2.5 0.25% 2500 9.60E+03 5.18E+09 5.18E+09 0.00E+00 <6% 2
CD11-40 40 527 50 3.88E+12 2.5 0.25% 2500 6.50E+03 7.74E+09 7.72E+09 2.24E+07 <4% 2
CD11-45 45 529 50 2.72E+12 2.5 0.25% 2500 4.50E+03 1.10E+10 1.08E+10 2.03E+08 <4% 2
CD11-50 50 531 50 1.99E+12 2.5 0.25% 2500 3.30E+03 1.51E+10 1.45E+10 5.88E+08 <4% 2
CD11-55 55 533 50 1.49E+12 2.5 0.25% 2500 2.50E+03 2.01E+10 1.88E+10 1.30E+09 <4% 2
CD11-60 60 536 50 1.15E+12 2.5 0.25% 2500 1.90E+03 2.61E+10 2.36E+10 2.48E+09 <4% 2
CD11-65 65 539 50 9.04E+11 2.5 0.25% 2500 1.50E+03 3.32E+10 2.89E+10 4.33E+09 <4% 2
CD11-70 70 542 50 7.23E+11 2.5 0.25% 2500 1.20E+03 4.15E+10 3.44E+10 7.09E+09 <4% 2
CD11-75 75 545 50 5.88E+11 2.5 0.25% 2500 9.80E+02 5.10E+10 4.00E+10 1.10E+10 <4% 2
CD11-80 80 549 50 4.85E+11 2.5 0.25% 2500 8.10E+02 6.19E+10 4.54E+10 1.65E+10 <4% 2
CD11-85 85 553 50 4.04E+11 2.5 0.25% 2500 6.70E+02 7.42E+10 5.03E+10 2.39E+10 <4% 2
CD11-90 90 558 50 3.40E+11 2.5 0.25% 2500 5.70E+02 8.81E+10 5.44E+10 3.37E+10 <4% 2
CD11-95 95 563 50 2.89E+11 2.5 0.25% 2500 4.80E+02 1.04E+11 5.72E+10 4.65E+10 <4% 2
CD11-100 100 569 50 2.48E+11 2.5 0.25% 2500 4.10E+02 1.21E+11 5.82E+10 6.27E+10 <4% 2
CD11-125 125 600 50 1.27E+11 2.5 0.25% 2500 2.10E+02 2.36E+11 1.53E+11 8.32E+10 <4% 2
CD11-150 150 612 50 7.35E+10 2.5 0.25% 2500 1.20E+02 4.08E+11 2.64E+11 1.44E+11 <4% 2

 

  Notes:
1
 Size accuracy +/- 0.1 nm
1
 Capping agent is covalently bonded carboxyl
2
 Size accuracy +/- 2 nm
3
 Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >=50 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Part# CD12 Gold Nanorods for Darkfield Imaging

 

Part # Diameter (nm) Length (nm) Peak SPR Wave (nm) Aspect Ratio OD SPR (AU) Peak LSPR Wave (nm) OD LSPR (AU) Nanorod Vol (nm3) Nanorods /mL Wt. conc (mg/ml) Wt. % PPM Molarity (nM) SPR Molar Ext. (M-1cm-1) LSPR Molar Ext. (M-1cm-1) Peak SPR accuracy (nm) SPR Linewidth 80% (nm)
CD12-10-2100 10 175 2100 17.5 5 510 1 1.35E+04 6.73E+11 0.175 0.02% 175 1.12 4.46E+09 8.92E+08 1900-2300 300
CD12-10-1400 10 102 1400 10.2 5 510 1 7.77E+03 1.17E+12 0.175 0.02% 175 1.95 2.57E+09 5.14E+08 1300-1500 150
CD12-10-1200 10 81 1200 8.1 5 510 1 6.13E+03 1.48E+12 0.175 0.02% 175 2.47 2.03E+09 4.05E+08 1132-1300 150
CD12-10-1064 10 67 1064 6.7 50 510 10 5.02E+03 1.81E+13 1.75 0.18% 1750 30.13 1.66E+09 3.32E+08 1022-1132 150
CD12-10-980 10 59 980 5.9 50 510 10 4.33E+03 2.09E+13 1.75 0.18% 1750 34.91 1.43E+09 2.86E+08 965-1022 150
CD12-10-950 10 55 950 5.5 50 510 10 4.09E+03 2.22E+13 1.75 0.18% 1750 37 1.35E+09 2.70E+08 925-965 150
CD12-10-900 10 50 900 5 50 510 10 3.68E+03 2.47E+13 1.75 0.18% 1750 41.11 1.22E+09 2.43E+08 875-925 150
CD12-10-850 10 45 850 4.5 50 510 10 3.27E+03 2.78E+13 1.75 0.18% 1750 46.25 1.08E+09 2.16E+08 829-875 100
CD12-10-808 10 41 808 4.1 50 510 10 2.93E+03 3.10E+13 1.75 0.18% 1750 51.68 9.68E+08 1.94E+08 794-829 75
CD12-10-780 10 38 780 3.8 50 510 10 2.70E+03 3.36E+13 1.75 0.18% 1750 56.06 8.92E+08 1.78E+08 765-794 65
CD12-10-750 10 35 750 3.5 50 510 10 2.45E+03 3.70E+13 1.75 0.18% 1750 61.67 8.11E+08 1.62E+08 725-765 50
CD12-10-700 10 29 700 2.9 50 510 10 2.04E+03 4.44E+13 1.75 0.18% 1750 74 6.76E+08 1.35E+08 675-725 40
CD12-10-650 10 24 650 2.4 50 510 10 1.64E+03 5.55E+13 1.75 0.18% 1750 92.5 5.41E+08 1.08E+08 625-675 40
CD12-10-600 10 19 600 1.9 50 510 10 1.23E+03 7.40E+13 1.75 0.18% 1750 123.34 4.05E+08 8.11E+07 575-625 40
CD12-25-1400 25 245 1400 9.8 5 514 1 1.16E+05 1.12E+11 0.25 0.03% 250 0.19 2.69E+10 5.37E+09 1232-1500 150
CD12-25-1064 25 137 1064 5.5 5 514 1 6.31E+04 2.05E+11 0.25 0.03% 250 0.34 1.46E+10 2.92E+09 1022-1232 150
CD12-25-980 25 119 980 4.8 50 514 10 5.43E+04 2.39E+12 2.5 0.25% 2500 3.98 1.26E+10 2.51E+09 965-1022 150
CD12-25-950 25 102 950 4.1 50 514 10 4.60E+04 2.82E+12 2.5 0.25% 2500 4.7 1.06E+10 2.13E+09 925-965 150
CD12-25-900 25 96 900 3.8 50 514 10 4.30E+04 3.01E+12 2.5 0.25% 2500 5.02 9.95E+09 1.99E+09 875-925 150
CD12-25-850 25 93 850 3.7 50 514 10 4.15E+04 3.12E+12 2.5 0.25% 2500 5.2 9.61E+09 1.92E+09 829-875 150
CD12-25-808 25 90 808 3.6 50 514 10 4.01E+04 3.24E+12 2.5 0.25% 2500 5.39 9.27E+09 1.85E+09 794-829 150
CD12-25-780 25 87 780 3.5 50 514 10 3.86E+04 3.36E+12 2.5 0.25% 2500 5.6 8.93E+09 1.79E+09 765-794 150
CD12-25-750 25 85 750 3.4 50 514 10 3.76E+04 3.45E+12 2.5 0.25% 2500 5.75 8.70E+09 1.74E+09 725-765 65
CD12-25-700 25 75 700 3 50 514 10 3.27E+04 3.96E+12 2.5 0.25% 2500 6.61 7.57E+09 1.51E+09 675-725 60
CD12-25-650 25 71 650 2.8 50 514 10 3.07E+04 4.22E+12 2.5 0.25% 2500 7.03 7.11E+09 1.42E+09 625-675 60
CD12-25-600 25 57 600 2.3 50 514 10 2.39E+04 5.43E+12 2.5 0.25% 2500 9.05 5.52E+09 1.10E+09 575-625 60
CD12-25-550 25 34 550 1.4 50 514 10 1.28E+04 1.01E+13 2.5 0.25% 2500 16.91 2.96E+09 5.91E+08 525-575 60
CD12-40-850 40 148 850 3.7 50 520 10 1.69E+05 7.67E+11 2.5 0.25% 2500 1.28 3.91E+10 7.83E+09 829-875 60
CD12-40-808 40 134 808 3.4 50 520 10 1.52E+05 8.56E+11 2.5 0.25% 2500 1.43 3.51E+10 7.01E+09 794-829 60
CD12-40-780 40 124 780 3.1 50 520 10 1.39E+05 9.33E+11 2.5 0.25% 2500 1.55 3.22E+10 6.43E+09 765-794 60
CD12-40-750 40 112 750 2.8 50 520 10 1.24E+05 1.05E+12 2.5 0.25% 2500 1.74 2.87E+10 5.73E+09 725-765 60
CD12-40-700 40 92 700 2.3 50 520 10 9.88E+04 1.31E+12 2.5 0.25% 2500 2.19 2.29E+10 4.57E+09 675-725 60
CD12-40-650 40 80 650 2 50 520 10 8.37E+04 1.55E+12 2.5 0.25% 2500 2.58 1.94E+10 3.87E+09 625-675 60
CD12-40-600 40 68 600 1.7 50 520 10 6.87E+04 1.89E+12 2.5 0.25% 2500 3.15 1.59E+10 3.18E+09 575-625 60
CD12-40-550 40 60 550 1.5 50 520 10 5.86E+04 2.21E+12 2.5 0.25% 2500 3.69 1.36E+10 2.71E+09 525-575 60
CD12-50-800 50 145 800 2.9 50 530 10 2.52E+05 5.15E+11 2.5 0.25% 2500 0.86 5.83E+10 1.17E+10 750-825 60
CD12-50-700 50 110 700 2.2 50 530 10 1.83E+05 7.08E+11 2.5 0.25% 2500 1.18 4.24E+10 8.48E+09 650-750 60
CD12-50-600 50 100 600 2 50 530 10 1.64E+05 7.93E+11 2.5 0.25% 2500 1.32 3.78E+10 7.57E+09 550-650 60

 

 

Functionalizations

 

Functionalized Nanorod Block Invitro

mutab

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer (top) and Nanopartz™ covalent MUTAB (bottom). Thickness of polymer is less than the size variablity of the gold nanoparticle.

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

Details

Composition

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

  1. Amine
  2. Biotin
  3. Carboxyl
  4. Neutravidin
  5. Streptavidin
  6. Azide

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Background

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

Technology Comparison

 

 

  Nanopartz™ Gold Nanoparticles for Dark Field Imaging Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

Features

  • Extremely stable. Shelf life is greater than six months.
  • Covalent bonds.
  • High anisotropy.
  • No cytotoxicity.
  • Nanorod to sphere shape changing on high energy pulsed lasers.
  • Bright Fluorescence - intense emission provides superior sensitivity and requires less conjugate.
  • Narrow Emission spectra - covalent bonds ensure no bleed through.
  • Excellent photostability - exceptional resistance to photobleaching enables fluorescence imaging uner the most demanding conditions.
  • Buffer Stability - stable from pH 4-9.
  • Instrument cability - excitation and emission spectra correspond with filter sets and laser setting of all popular fluorescence instrumentation.

Benefits

  • Customer can focus on research and not on Nanopartz™ expertise
  • Quick turnaround

 

Spherical Gold UV VIS Gold Nanorods UV VIS

 

 

Spherical Gold UV VIS

Photomicrographs of Chlamydomonas are shown in Figure 2A-C and a buccal cell in Figure 2D. The difference in refractive indices of the media with the cell wall and other components of the algae are visualized in brightfield (Fig. 2A & B). Closing the condenser aperture diaphragm increases contrast allowing the flagella to be barely visible (Fig. 2A). Darkfield clearly shows many intracellular details as well as the flagella (Fig. 2C). Because these are unmounted living cells, they have moved a bit between the different exposures. Buccal cells are so similar in refractive index to the medium that photos of them could not be obtained in brightfield. Figure 2D shows buccal cells in darkfield; nucleus and other intracellular structures are clearly visible. Samples were photographed using CH2 student-grade Olympus microscope with 40X objective and 3.3X photo eyepiece on Kodak TMAX 400 film.

Gold Nanorods UV VIS

This diagram compares the essential components of brightfield and darkfield microscopy. The difference in illumination (shown by stippling) of the sample between brightfield and darkfield is emphasized in the diagram. Darkfield utilizes a darkfield "stop" illustrated by the "spider stop" placed below the condenser. This stop blocks the center of the beam of light to produce a hollow cone of light. This light does not directly enter the objective lens. In contrast, a solid cone of light illuminates and enters the objective lens in brightfield. Only light that is scattered by the sample (depicted by the lines in the diagram) and enters the objective lens is seen as an image in darkfield.

 

 

FAQz

 

Testimonials

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

Three dimensional orientational imaging of nanoparticles with darkfield microscopy

L Xiao, YX Qiao, Y He, ES Yeung - Analytical chemistry, 2010 - ACS Publications
... molecules.(20-27) One of them is polarization imaging which uses dark-field microscopy when
combined with ... 25 nm and longitudinal length 60 nm were purchased from Nanopartz (Loveland,
CO). ... Therefore, the field distribution on the darkfield image reflects not only the three ...

 

Plasmonic nanorod absorbers as orientation sensors

WS Chang, JW Ha, LS Slaughter… - Proceedings of the …, 2010 - National Acad Sciences
... In addition, the much larger sensitivity of photothermal imaging compared to dark-field scattering
spectroscopy will ... This pattern aided in locating the same particles in SEM and optical microscopy. ...
700), and 86-nm (part #30-25-750) long Au NRs were obtained from Nanopartz. ...

 

Resolving rotational motions of nano-objects in engineered environments and live cells with gold nanorods and differential interference contrast microscopy

G Wang, W Sun, Y Luo, N Fang - Journal of the American …, 2010 - ACS Publications
... with ϕ: It is worthwhile to mention that the relative brightness and relative darkness are measures ...
3D is directly related to the signal-to-noise ratio of the bright/dark intensities, which ... demonstrate
that by using the combination of gold nanorod probes and DIC microscopy, we are ...

 

Enhancing the optical excitation efficiency of a single self-assembled quantum dot with a plasmonic nanoantenna

M Pfeiffer, K Lindfors, C Wolpert, P Atkinson… - Nano …, 2010 - ACS Publications
... with a diameter of 90 nm as specified by the manufacturer (Nanopartz Inc.). ... with markers so that
the same area can be studied with AFM and optical microscopy. ... were characterized by recording
the scattering spectrum of single antennas using dark-field microspectroscopy.(19 ...

 

Wide‐field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy

S Patskovsky, E Bergeron, D Rioux… - Journal of …, 2015 - Wiley Online Library
... signal-to-noise ratio for NPs biomarkers visualization is provided by a dark-field microscopy
[4]. This ... Toronto, ON) were tethered to AuNPs (50 µg/mL, 100 nm diameter, Nanopartz, A11-100 ...
of these functions is about 0.5–0.8 µm for reflected light imaging mode (black line) and at ...

 

Optical imaging of cancer heterogeneity with multispectral optoacoustic tomography

E Herzog, A Taruttis, N Beziere, AA Lutich, D Razansky… - Radiology, 2012 - pubs.rsna.org
... Gold nanorods (AuNR-M; Nanopartz, Loveland, Colo) with an optical absorption peak ... through
the tumors were characterized by using an upright dark-field microscope (Axiotech; Carl ... At
dark-field microscopy, single nanorods and agglomerations thereof were seen as bright ...

 

Wavelength-dependent differential interference contrast microscopy: selectively imaging nanoparticle probes in live cells

W Sun, G Wang, N Fang, ES Yeung - Analytical chemistry, 2009 - ACS Publications
... Dark-field microscopies have gained much attention due to their high signal strength and ... that
were used to conjugate with TAT peptide were purchased from Nanopartz (Salt Lake ... The size
distributions were measured by transmission electron microscopy, and they agreed well ...

 

Waveguide scattering microscopy for dark-field imaging and spectroscopy of photonic nanostructures

DJ Hill, CW Pinion, JD Christesen, JF Cahoon - Acs Photonics, 2014 - ACS Publications
... microscopy (S-WSM) and coverslip waveguide scattering microscopy (C-WSM), respectively,
light scattered out of the waveguide by the sample is collected with an objective lens to form a
dark-field image. We implemented WSM on a standard upright microscope (Zeiss Axio ...

 

Elastic interactions between colloidal microspheres and elongated convex and concave nanoprisms in nematic liquid crystals

B Senyuk, II Smalyukh - Soft Matter, 2012 - pubs.rsc.org
... and 500 × 100 nm, respectively, were obtained as ethanol-based dispersions from Nanopartz,
Inc ... Double white and black arrows show the far-field director n 0 . Double red arrow ... setup built
around an inverted Olympus microscope IX81 was used to perform dark-field, bright-field ...

 

Gold Nanoparticle‐Loaded Neural Stem Cells for Photothermal Ablation of Cancer

K Schnarr, R Mooney, Y Weng, D Zhao… - Advanced …, 2013 - Wiley Online Library
... serum albumin was used as a negative control for chemotaxis (black bar); unloaded NSCs ... AuNRs)
with longitudinal plasmon resonances at 810nm were supplied by Nanopartz, a division ... Dark
Field Imaging of MUTAB-AuNR Uptake by NSCs: Dark field microscopy was used in ...