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Conjugated Nanopartz™ Gold Nanoparticles

Nanopartz™ Gold Nanoparticles, conjugated specifically for in vitro applications, utilize the complete line of Accurate™ Spherical Gold Nanoparticles, Nanorods, Microgold™, Gold Nanowires, and Plasmonic Fluorophores™ conjugated via a covalent polymer bridge to the most popular in vitro terminations including amine, biotin, carboxyl, methyl, maleimide, neutravidin, nhs, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. Most importantly, Nanopartz™ posseses the proprietary technology to bind to almost any location of the target. For instance, in binding to antibodies, not only does Nanopartz™ conjugate to the antibody, but can to specific sections of the antibody. This ability to covalently conjugate to specific locations ensures the activity of the target is not compromised. These conjugated particles can be used in brightfield, darkfield, fluorescence, and other imaging modes; and ELISA, lateral flow, and other assays. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the target is determined.

 

  • Summary
  • Background
  • Details
  • Images/Videos
  • Technical Notes
  • MSDS/FAQz
 

Conjugated Spherical Gold Nanoparticles for in vitro (OD50/2.5mg in 1mL)

Part # Diam (nm) NPS/mL
C11-1.8 1.8 4.4E+16
C11-2.2 2.2 2.4E+16
C11-3 3 9.6E+15
C11-4 4 4.1E+15
C11-5 5 2.1E+15
C11-10 10 2.7E+14
C11-15 15 8.0E+13
C11-20 20 3.4E+13
C11-25 25 1.8E+13
C11-30 30 1.0E+13
C11-35 35 6.5E+12
C11-40 40 4.3E+12
C11-45 45 3.1E+12
C11-50 50 2.2E+12
C11-55 55 1.7E+12
C11-60 60 1.3E+12
C11-65 65 1.0E+12
C11-70 70 8.2E+11
C11-75 75 6.7E+11
C11-80 80 5.5E+11
C11-85 85 4.6E+11
C11-90 90 3.9E+11
C11-95 95 3.3E+11
C11-100 100 2.9E+11
C11-125 125 1.5E+11

Conjugated Gold Nanorodz™ for in vitro (OD 50 in 1mL)

Part #  Diam (nm) Long Size (nm) Peak SPR Wave (nm) NPS/mL Wt. conc (mg) (10-3) A/R
C12-10-1064 10 67 1064 1.81E+13 1750 6.7
C12-10-980 10 59 980 2.09E+13 1750 5.9
C12-10-900 10 50 900 2.47E+13 1750 5.0
C12-10-850 10 45 850 2.78E+13 1750 4.5
C12-10-808 10 41 808 3.10E+13 1750 4.1
C12-10-780 10 38 780 3.36E+13 1750 3.8
C12-10-750 10 35 750 3.70E+13 1750 3.5
C12-10-700 10 29 700 4.44E+13 1750 2.9
C12-25-980 25 146 980 1.91E+12 2500 5.9
C12-25-850 25 113 850 2.54E+12 2500 4.5
C12-25-808 25 102 808 2.83E+12 2500 4.1
C12-25-780 25 94 780 3.08E+12 2500 3.8
C12-25-750 25 86 750 3.38E+12 2500 3.5
C12-25-700 25 73 700 4.06E+12 2500 2.9
C12-25-650 25 60 650 5.07E+12 2500 2.4
C12-25-600 25 47 600 6.77E+12 2500 1.9
C12-25-550 25 34 550 1.01E+13 2500 1.4
C12-40-750 40 138 750 8.26E+11 2500 3.5
C12-40-700 40 118 700 9.91E+11 2500 2.9
C12-40-650 40 97 650 1.24E+12 2500 2.4
C12-40-600 40 76 600 1.65E+12 2500 1.9
C12-40-550 40 55 550 2.48E+12 2500 1.4
C12-50-700 50 147 700 5.07E+11 2500 2.9

Conjugated Microgold for in vitro

Part # Diam (nm) Length (nm) NPS/ml Wt. conc (mg/ml) Molarity (pM)
C13-500 75 500 5.87E+09 0.25 9.79E+00
C13-1000 100 1000 1.65E+09 0.25 2.75E+00

Conjugated Gold Nanowires for in vitro

Part # Diam(nm) Length(nm) mg/mL AR nps/mL
C14-1000 75 1000 0.25 13 2.94E+06
C14-2000 75 2000 0.25 27 1.47E+06
C14-3000 75 3000 0.25 40 9.79E+05
C14-4000 75 4000 0.25 53 7.34E+05
C14-6000 75 6000 0.25 80 4.89E+05
C14-7500 75 7500 0.25 100 3.92E+05
C14-10000 75 10000 0.25 133 2.94E+05

Plasmonic Fluorophores™ (2.5mg/mL, OD 50)

Typical Rod/Fluor equivalent is 1 rod vs. 1 fluorophore enchancement

Part #  Diam (nm) Long Size (nm) Peak SPR Wave (nm) Ex/Em (nm) NPS/ml Wt. conc (mg/ml) (10-3) Wt. % PPM Molar (pM) SPR Molar Ext. (M-1cm-1)  Typ. Rod/ Fluor Equiv Spectrally Similar Dyes
C16-25-800 25 99 800 777/794 3.49E+12 2500 0.25% 2500 5.81 8.61E+12 50000 IRDye 800
C16-25-750 25 86 750 754/776 4.21E+12 2500 0.25% 2500 7.02 7.13E+12 50000 Alexa Fluor 750
C16-25-680 25 68 680 692/712 5.93E+12 2500 0.25% 2500 9.88 5.06E+12 50000 Alexa Fluor 680, Cy5.5
C16-25-650 25 60 650 652/672 7.19E+12 2500 0.25% 2500 12.0 4.17E+12 40000 Alexa Fluor 647, Cy5
C16-25-633 25 56 633 638/658 8.18E+12 2500 0.25% 2500 13.6 3.67E+12 40000 Alexa Fluor 633
C16-25-600 25 46 594 593/618 1.19E+13 2500 0.25% 2500 19.9 2.52E+12 35000 Alexa Fluor 594, Texas Red
C16-25-550 25 33 546 562/576 2.73E+13 2500 0.25% 2500 45.6 1.10E+12 35000 Alexa Fluor 546, Alexa Fluor 555

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

Background

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

Technology Comparison

  Nanopartz in vitro Gold Nanoparticles Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

Highlights

  • Covalent bonds insure specificity, stability, long shelf life.
  • Buffer Stability - stable from pH 4-9.
  • No sodium azide.
  • No BSA.
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding.

Testimonials

“ We have shown by Western Blotting that we have successfully used the Nanopartz GSH gold particles to isolate proteins from cell lysates. We are very pleased with the Nanopartz GSH gold as they have worked incredibly well.”

Dr Michelle Hughes
MRC Toxicology Unit
University of Leicester

 

 

in vitro gold nanoparticle with Nanopartz™ covalently bonded polymer (left) and Nanopartz™ covalent MUTAB (right). Thickness of polymer is less than the size variablity of the gold nanoparticle.

 

Composition

These in vitro gold nanoparticles for in vitro dark field, bright field, and fluorescence imaging come in numerous conjugations:

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

  1. Amine
  2. Biotin
  3. Carboxy
  4. Maleimidel
  5. Methyl
  6. Neutravidin
  7. NHS
  8. Streptavidin
  9. Azide
  10. MUTAB

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM
  6. Protein A

Custom Conjugations are also available including HRP and GSH.

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Microgold (Part #C75-1000-PU) with MUTAB coating under brightfield microscope, cancer cell, 100x objective

 

 

C15, C35, C55, C75, and C85 MSDS

FAQz

What is the thickness of the polymer layer?

It is less than the variability of the size of the particle and therefore unmeasurable.

 

Home » C- Nanoparticles for In-Vitro » C- Conjugated Gold Nanoparticles for In-Vitro

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Conjugated Spherical Gold Nanoparticles for In-Vitro
Conjugated Spherical Gold Nanoparticles for In-Vitro
 
C11-(SIZE)-(CONJUGATION)-(OD-mLs); this product replaces the 20-P series products.
:
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $440.00

Custom Oligo Conjugated Spherical Gold Nanoparticles for In Vitro
Custom Oligo Conjugated Spherical Gold Nanoparticles for In Vitro
 
Part# C11L-(SIZE)-(OLIGO)-(OD-mLs) Up to 30BPs, up to 1mcM of oligo
:
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $640.00

Custom Conjugated Spherical Gold Nanoparticles for In Vitro
Custom Conjugated Spherical Gold Nanoparticles for In Vitro
 
Part# C11R-(SIZE)-(TERM GROUP)-(OD-mLs) This product requires a quote. Please enter the following parameters and add to cart. Complete your contact information and submit for quote.
:
:
:
Sterilization: Certified Sterilized
Endotoxin: Endotoxin Purified
Endotoxin Purified and Certified
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $0.00

Conjugated Gold Nanorods for In-Vitro
Conjugated Gold Nanorods for In-Vitro
 
C12-(DIAM/SPR)-(CONJUGATION)-(OD-mLs); this product replaces the 30-P and 30-S series products.
:
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

Custom Oligo Conjugated Nanorods for In Vitro
Custom Oligo Conjugated Nanorods for In Vitro
 
Part# C12L-(DIAM/SPR)-(OLIGO)-(OD-mLs) Up to 30BP, 1mcM of oligo
:
:
:
Sterilization: Certified Sterilized
Endotoxin: Endotoxin Purified
Endotoxin Purified and Certified
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $640.00

Custom Conjugated Nanorods for In Vitro
Custom Conjugated Nanorods for In Vitro
 
Part# C12R-(DIAM/SPR)-(TERM GROUP)-(OD-mLs) This product requires a quote. Please enter the following parameters and add to cart. Complete your contact information and submit for quote.
:
:
:
Sterilization: Certified Sterilized
Endotoxin: Endotoxin Purified
Endotoxin Purified and Certified
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $0.00

Conjugated Microgold for In-Vitro
Conjugated Microgold for In-Vitro
 
C13-(LENGTH)-(CONJUGATION); 0.25mg in 1mL
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

Conjugated Gold Nanowires for In-Vitro
Conjugated Gold Nanowires for In-Vitro
 
C14-(LENGTH)-(CONJUGATION); 0.25mg in 1mL
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

Conjugated In-Vitro Plasmonic Fluorophores
Conjugated In-Vitro Plasmonic Fluorophores
 
Part #C16-(FLUOR)-(CONJUGATION)-(OD-mLs)
:
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $440.00

Conjugated In-Vitro Ramanprobes
Conjugated In-Vitro Ramanprobes
 
Part #C17-(Wavelength)-(Label)-(CONJUGATION)-(OD-mLs)
:
:
:
:
Sterilization: Certified Sterilized (+$150.00)
Endotoxin: Endotoxin Purified and Certified (+$250.00)
Negative Control: 0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00