Conjugated Nanopartz™ Gold Nanoparticles
Nanopartz™ Gold Nanoparticles, conjugated specifically for in vitro applications, utilize the complete line of Accurate™ Spherical Gold Nanoparticles, Nanorods, Microgold™, Gold Nanowires, and Plasmonic Fluorophores™ conjugated via a covalent polymer bridge to the most popular in vitro terminations including amine, biotin, carboxyl, methyl, maleimide, neutravidin, nhs, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. Most importantly, Nanopartz™ posseses the proprietary technology to bind to almost any location of the target. For instance, in binding to antibodies, not only does Nanopartz™ conjugate to the antibody, but can to specific sections of the antibody. This ability to covalently conjugate to specific locations ensures the activity of the target is not compromised. These conjugated particles can be used in brightfield, darkfield, fluorescence, and other imaging modes; and ELISA, lateral flow, and other assays. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the target is determined.
- Summary
- Background
- Details
- Images/Videos
- Technical Notes
- MSDS/FAQz
Conjugated Spherical Gold Nanoparticles for in vitro (OD50/2.5mg in 1mL)
Conjugated Gold Nanorodz™ for in vitro (OD 50 in 1mL)
Conjugated Microgold for in vitro
Conjugated Gold Nanowires for in vitro
Plasmonic Fluorophores™ (2.5mg/mL, OD 50)Typical Rod/Fluor equivalent is 1 rod vs. 1 fluorophore enchancement
Conjugations (Ordering Abbreviation)
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Background
These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.
Technology Comparison
| Nanopartz™ in vitro Gold Nanoparticles | Citrate spherical gold nanoparticles with adsorbed conjugates | |
| Stability (salt,pH,chemicals) | High | Low |
| Scattering linewidth | Narrow (monodisperse) | Broad (polydisperse) |
| Multilabeling | Yes (500nm-2100nm) | Limited (520nm-600nm) |
| Nonspecific binding | Very low | High |
| pH and salt resistance | High | Medium |
| Sterilization | Radiation | Sodium Azide |
| Site specific antibody conjugation | Yes | No |
Highlights
- Covalent bonds insure specificity, stability, long shelf life.
- Buffer Stability - stable from pH 4-9.
- No sodium azide.
- No BSA.
- Polymer coating insures no aggregation in high salts, reduced nonspecific binding.
Testimonials
“ We have shown by Western Blotting that we have successfully used the Nanopartz GSH gold particles to isolate proteins from cell lysates. We are very pleased with the Nanopartz GSH gold as they have worked incredibly well.”
Dr Michelle Hughes
MRC Toxicology Unit
University of Leicester
in vitro gold nanoparticle with Nanopartz™ covalently bonded polymer (left) and Nanopartz™ covalent MUTAB (right). Thickness of polymer is less than the size variablity of the gold nanoparticle.
Composition
These in vitro gold nanoparticles for in vitro dark field, bright field, and fluorescence imaging come in numerous conjugations:
Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.
- Amine
- Biotin
- Carboxy
- Maleimidel
- Methyl
- Neutravidin
- NHS
- Streptavidin
- Azide
- MUTAB
Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.
- Goat Anti-Human IgG
- Goat Anti-Mouse IgG
- Goat Anti-Rabitt IgG
- Goat Anti-Human IgA
- Goat Anti-Human IgM
- Protein A
Custom Conjugations are also available including HRP and GSH.
Quantity
This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.
Delivery
All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.
Shelf Life/Storage Temperature
This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.
Sterilization/Endotoxin
Sterilization and Endoxtoin Free Options are available.
Certifications
This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
Microgold (Part #C75-1000-PU) with MUTAB coating under brightfield microscope, cancer cell, 100x objective 
TN802 Photothermal Characteristics of Gold Nanorods
TNC01 Overview of Gold Nanorods
ANC01 GSH Conjugated Gold Nanoparticles for pulling out GST proteins from Bacterial Lysates
C15, C35, C55, C75, and C85 MSDS
FAQz
What is the thickness of the polymer layer?
It is less than the variability of the size of the particle and therefore unmeasurable.
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