Nanopartz™ Accurate™ Spherical Gold Nanoparticles for Intrinsic Raman Scattering

Nanopartz™ Accurate™ Spherical Gold Nanoparticles are spherical gold nanoparticles manufactured using patented, patent pending, and proprietary methods that result in exact sizes, highly monodisperse sizes and shapes, long shelf lives, along with significantly improved reactivity. Diameters from 1.8nm to 125nm with size accuracies up to 0.1nm and size variances less than 4% at sizes larger than 40nm. Peak SPRs range from 505nm to 570nm. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data.

Utilizing its natural negative charge, high k-value for binding, these particles are perfect for enhancing the Raman signal of added analytes. This product comes highly concentrated in 1mL vials, ready for dilution in water.

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Accurate Concentrated SGNPs  1.8-150nm
Accurate Concentrated SGNPs 1.8-150nm
Part# AC11-(SIZE)-(LIGAND)-(VOL)-DI Water
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Price: $400.00
Accurate SGNPs 10-90nm 1mL KIT
Accurate SGNPs 10-90nm 1mL KIT
Part# AK11-(SIZE)-1mL; Minimum Qty. 8, 1mL ea. in 18MEG DI Water; Enter Qty. for each size. Only for online orders.
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
Price: $0.00

 

 

Part #
Diam (nm)
Peak SPR (nm)
Optical Density (1cm)
NPS/ml
Wt. conc (ug/ml)
Wt. %
PPM
Molarity (pM)
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Notes
A11-1.8
1.8
505-508
1
8.38E+14
50
0.01%
50
1.40E+06
7.16E+05
7.16E+05
0.00E+00
<35%
1,3
A11-2.2
2.2
505-508
1
4.59E+14
50
0.01%
50
7.65E+05
1.31E+06
1.31E+06
0.00E+00
<25%
1,3
A11-3
3
505-508
1
1.81E+14
50
0.01%
50
3.02E+05
3.32E+06
3.32E+06
0.00E+00
<20%
1,3
A11-4
4
505-508
1
7.64E+13
50
0.01%
50
1.27E+05
7.86E+06
7.86E+06
0.00E+00
<20%
1,3
A11-5
5
505-508
1
3.91E+13
50
0.01%
50
6.52E+04
1.53E+07
1.53E+07
0.00E+00
<20%
2,3
A11-10
10
514-520
1
4.89E+12
50
0.01%
50
8.14E+03
1.23E+08
1.23E+08
0.00E+00
<15%
2,3
A11-15
15
514-520
1
1.45E+12
50
0.01%
50
2.41E+03
4.14E+08
4.14E+08
0.00E+00
<15%
2,3
A11-20
20
514-520
1
6.11E+11
50
0.01%
50
1.02E+03
9.82E+08
9.82E+08
0.00E+00
<10%
2,3
A11-25
25
514-520
1
3.13E+11
50
0.01%
50
521
1.92E+09
1.92E+09
0.00E+00
<10%
2
A11-30
30
523
1
1.81E+11
50
0.01%
50
302
3.32E+09
3.32E+09
0.00E+00
<6%
2
A11-35
35
526
1
1.14E+11
50
0.01%
50
190
5.26E+09
5.26E+09
0.00E+00
<6%
2
A11-40
40
527
1
7.64E+10
50
0.01%
50
127
7.86E+09
7.84E+09
2.28E+07
<4%
2
A11-45
45
529
1
5.36E+10
50
0.01%
50
89.4
1.12E+10
1.10E+10
2.06E+08
<4%
2
A11-50
50
531
1
3.91E+10
50
0.01%
50
65.2
1.53E+10
1.48E+10
5.97E+08
<4%
2
A11-55
55
533
1
2.94E+10
50
0.01%
50
49
2.04E+10
1.91E+10
1.32E+09
<4%
2
A11-60
60
536
1
2.26E+10
50
0.01%
50
37.7
2.65E+10
2.40E+10
2.52E+09
<4%
2
A11-65
65
539
1
1.78E+10
50
0.01%
50
29.7
3.37E+10
2.93E+10
4.40E+09
<4%
2
A11-70
70
542
1
1.42E+10
50
0.01%
50
23.7
4.21E+10
3.49E+10
7.20E+09
<4%
2
A11-75
75
545
1
1.16E+10
50
0.01%
50
19.3
5.18E+10
4.06E+10
1.12E+10
<4%
2
A11-80
80
549
1
9.54E+09
50
0.01%
50
15.9
6.29E+10
4.61E+10
1.68E+10
<4%
2
A11-85
85
553
1
7.96E+09
50
0.01%
50
13.3
7.54E+10
5.11E+10
2.43E+10
<4%
2
A11-90
90
558
1
6.70E+09
50
0.01%
50
11.2
8.95E+10
5.52E+10
3.43E+10
<4%
2
A11-95
95
563
1
5.70E+09
50
0.01%
50
9.5
1.05E+11
5.81E+10
4.72E+10
<4%
2
A11-100
100
569
1
4.89E+09
50
0.01%
50
8.1
1.23E+11
5.91E+10
6.37E+10
<4%
2
A11-125
125
600
1
2.50E+09
50
0.01%
50
4.2
2.40E+11
1.29E+10
2.27E+11
<4%
2
A11-150
150
612
1
1.45E+09
50
0.01%
50
2.4
4.14E+11
-2.07E+11
6.21E+11
<4%
2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >= 1 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

 

 

 

Part #
Diameter (nm)
Peak SPR (nm)
Optical Density (1cm)
NPS/ml
Wt. conc (mg/ml)
Wt. %
PPM
Molarity (pM)
Molar Ext. (M-1cm-1)
Absorption Molar Ext. (M-1cm-1)
Scattering Molar Ext. (M-1cm-1)
Size Dispersity %PDI
Notes
AC11-1.8
1.8
505-508
100
8.38E+16
5
0.50%
5000
1.40E+08
7.16E+05
7.16E+05
0.00E+00
<35%
1,3
AC11-2.2
2.2
505-508
100
4.59E+16
5
0.50%
5000
7.65E+07
1.31E+06
1.31E+06
0.00E+00
<25%
1,3
AC11-3
3
505-508
100
1.81E+16
5
0.50%
5000
3.02E+07
3.32E+06
3.32E+06
0.00E+00
<20%
1,3
AC11-4
4
505-508
100
7.64E+15
5
0.50%
5000
1.27E+07
7.86E+06
7.86E+06
0.00E+00
<20%
1,3
AC11-5
5
505-508
100
3.91E+15
5
0.50%
5000
6.52E+06
1.53E+07
1.53E+07
0.00E+00
<20%
2,3
AC11-10
10
514-520
100
4.89E+14
5
0.50%
5000
8.14E+05
1.23E+08
1.23E+08
0.00E+00
<15%
2,3
AC11-15
15
514-520
100
1.45E+14
5
0.50%
5000
2.41E+05
4.14E+08
4.14E+08
0.00E+00
<15%
2,3
AC11-20
20
514-520
100
6.11E+13
5
0.50%
5000
1.02E+05
9.82E+08
9.82E+08
0.00E+00
<10%
2,3
AC11-25
25
514-520
100
3.13E+13
5
0.50%
5000
5.21E+04
1.92E+09
1.92E+09
0.00E+00
<10%
2
AC11-30
30
523
100
1.81E+13
5
0.50%
5000
3.02E+04
3.32E+09
3.32E+09
0.00E+00
<6%
2
AC11-35
35
526
100
1.14E+13
5
0.50%
5000
1.90E+04
5.26E+09
5.26E+09
0.00E+00
<6%
2
AC11-40
40
527
100
7.64E+12
5
0.50%
5000
1.27E+04
7.86E+09
7.84E+09
2.28E+07
<4%
2
AC11-45
45
529
100
5.36E+12
5
0.50%
5000
8.94E+03
1.12E+10
1.10E+10
2.06E+08
<4%
2
AC11-50
50
531
100
3.91E+12
5
0.50%
5000
6.52E+03
1.53E+10
1.48E+10
5.97E+08
<4%
2
AC11-55
55
533
100
2.94E+12
5
0.50%
5000
4.90E+03
2.04E+10
1.91E+10
1.32E+09
<4%
2
AC11-60
60
536
100
2.26E+12
5
0.50%
5000
3.77E+03
2.65E+10
2.40E+10
2.52E+09
<4%
2
AC11-65
65
539
100
1.78E+12
5
0.50%
5000
2.97E+03
3.37E+10
2.93E+10
4.40E+09
<4%
2
AC11-70
70
542
100
1.42E+12
5
0.50%
5000
2.37E+03
4.21E+10
3.49E+10
7.20E+09
<4%
2
AC11-75
75
545
100
1.16E+12
5
0.50%
5000
1.93E+03
5.18E+10
4.06E+10
1.12E+10
<4%
2
AC11-80
80
549
100
9.54E+11
5
0.50%
5000
1.59E+03
6.29E+10
4.61E+10
1.68E+10
<4%
2
AC11-85
85
553
100
7.96E+11
5
0.50%
5000
1.33E+03
7.54E+10
5.11E+10
2.43E+10
<4%
2
AC11-90
90
558
100
6.70E+11
5
0.50%
5000
1.12E+03
8.95E+10
5.52E+10
3.43E+10
<4%
2
AC11-95
95
563
100
5.70E+11
5
0.50%
5000
949.9
1.05E+11
5.81E+10
4.72E+10
<4%
2
AC11-100
100
569
100
4.89E+11
5
0.50%
5000
814.5
1.23E+11
5.91E+10
6.37E+10
<4%
2
AC11-125
125
600
100
2.50E+11
5
0.50%
5000
417
2.40E+11
1.29E+10
2.27E+11
<4%
2
AC11-150
150
612
100
1.45E+11
5
0.50%
5000
241.3
4.14E+11
-2.07E+11
6.21E+11
<4%
2

 

  Notes:
1
Size accuracy +/- 0.1 nm
1
Capping agent is covalently bonded carboxyl
2
Size accuracy +/- 2 nm
3
Peak SPR does not determine size for d<20 nm
Vol is 25mL, 100mL, 500mL, 1000mL
%PDI = Std Dev/Size
See Tech Note TN801 for definition of terms and method of analysis
All specs typical. May vary batch to batch. Exact values are measured for each batch
OD >= 1 for all sizes
Shape monodispersity (% spheres) > 99.9%
Solution is 18MEG DI water
Residual Chemicals < 0.1%

 

 

Nanopartz carboxylic acid capped gold nanoparticle

Proprietary Carboxylic Acid (<300Da)

 

 

 

SGNPS

Citrate

 

 

 

 

 

Composition

These bare spherical gold nanoparticles are shipped in  DI water with no measurable residual chemicals. Two ionically adsorbed ligands are available, our proprietary carboxylic acid, and citrate. Both strongly negatively charged for absorption to analytes.

Custom Formulation

Diameters 1.8nm to 150nm.  Other sizes are special order.  Please contact us.

Quantity

This product is available in 1mL and larger.

Introductory Kits

For smaller evaluation volumes, two different kits are available, one with 25mL volume with a four bottle minimum and one with 1mL with an 8 bottle minimum.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product. For our dried particles, storage is room temperature with a shelf life of 1 year.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

The manufacturing method for these nanoparticles are based on our patented method. This invention allowed the first use of a reducing agent to be used at room temperature. Unlike citrate which is only a reducing agent at the boiling temperature of water - thereby quickly making non-monodisperse gold nanoparticles, our reducing agent works by slowing building nanoparticles from seed, atom by atom, over the course of up to twelve days. The result is a highly monodisperse product with unrivaled spherical shapes.

We exclusively licensed this technology in 2006 and have since improved the ability to ligand exchange our nanoparticles with citrate and a proprietary postively charged ligand. Citrate offers the customer a known ligand, but with the benefit of a highly monodisperse product.

  Nanopartz™Accurate™ Spherical Gold Nanoparticles Other Technologies
Monodispersity High (<2%) Low (>15%)
Size Accuracy High (+/-2nm) Low
Linewidths Narrow Broad
Storage lifetime Years 6 months at 4C
Ability to ligand exchange Easy More difficult

 

  • Highly accurate and monodisperse in size, shape, and batch to batch uniformity.
  • Accurate to +/- 2 nm in diameter.
  • Size monodispersity better than 4% CV for many sizes.
  • Greater than 99.9% spheres.
  • Carboxylic acid capping agent is reduced to < 0.1% without adverse effects to the spheres.
  • Extremely stable.  Shelf life is greater than one year.  May be stored at room temperature for extended periods.
  • The light scattering power of a 60 nm gold particle is equivalent to ~5 X 105 fluorescein molecules.
  • Accurate™ SGNPs do not photobleach.
  • Light scatter can be detected by the unaided eye at particle concentrations as low as 10-15 M (femtomolar) in suspension and as low as 0.005 particles/m2 on a transparent surface.
  • Individual gold nanoparticles can be seen by eye in an inexpensive student microscope with simple dark field illumination allowing for very sensitive and inexpensive particle counting assays.
  • Different sized particles scatter different colors of light and therefore can be used as multicolor multiplexed labels.
  • Facile conjugations allow for coating of antibodies, receptors, ligands, DNA without affecting their light scattering properties.
  • Slightly negatively charged (reduced aggregation).
  • Facile synthesis.  Gold chemistry is well known.
  • No known cytotoxicity.  Gold is FDA approved for rheumatoid arthritis.
  • Monodisperse shapes and sizes in production volumes satisfies high volume applications.
  • Smaller negative charge reduces aggregation.
  • Reduced number of additives improves binding to antibodies, receptors, ligands and DNA.
  • Monodisperse sizes and shapes leads to better standards for electron microscopy.
  • No photobleaching means better diagnostic labels with the ability to archive.
  • 105 better scattering power than fluorescein means lower limits of detection with simpler instrumentation.
  • More accurate sizing makes for better SPR matching for Surface Enhanced Raman Labels.
  • Monodisperse sizes and shapes leads to better Resonance Light Scattering labels in sensitivity and multiplexing.

 

Why are your spherical gold nanoparticles so much more monodisperse than conventional citrate stabilized gold nanoparticles?

Citrate stabilized gold nanoparticles are manufactured by combining sodium citrate and hydrogen tetrachloroaurate (gold) in water. In this combination, citrate not only acts as the capping agent, it also reduces the gold to a charge state that allows nanoparticles to form. However, citrate is only a reducing agent at the boiling point of water - so the solution must be boiled. Nanoparticle monodispersity is dependent on an even, homogeneous concentration of the chemical components in solution, and because boiling introduces heterogeneity into the process, the result is a very heterogeneous product.

Our capping agent is similar to citrate and is added to the hydrogen tetrachloroaurate in water like the citrate process. However our capping agent is a reducing agent at room temperature. Consequently, by slowly adding gold over time, we can control the growth, shape, and monodispersity of the gold nanoparticles to within 2nm accuracies.

Why are your citrate stabilized gold nanoparticles so much more monodisperse than conventional gold nanoparticles?

We use our capping agent to manufacture the highly monodisperse gold nanoparticles and then ligand exchange our capping agent with citrate. Since the binding energy (k) for citrate is so much stronger than our capping agent, our capping agent is readily replaced.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

Many companies claim better than 10% PDI using citrate. Is this possible?

We find that hard to believe.

Why is your shelf life time so much longer for your proprietary gold nanoparticles when compared to citrate stabilized gold nanoparticles?

As opposed to citrate, our capping agent is not a growth medium.

Are your proprietary gold nanoparticles interchangeable with citrate stabilized gold nanoparticles?

Yes. However, the user must be aware that in order to stabilize citrate gold nanoparticles, previous manufacturers used to add many stabilizing agents. Consequently for example to ionically attach ligands to gold nanoparticles required the chemist to "drive" the reaction by adding 10-1000 times greater concentrations of the ligand. This is no longer necessary with our gold nanoparticles.

 

"We were previously making our own gold nanoparticles using the commonly used technique of citrate reduction of Au(III). The colloids generated are broadly polydisperse, and we were concerned that our application of these particles in immuno-gold labeling of cells for optical coherence microscopy (OCM) would be utilizing only the large diameter tail of the distribution. We purchased the sampler package of NanoPartz gold spheres (30 nm, 50 nm, 70 nm, 90 nm) and examined each size carefully with dynamic light scattering, OCM, and spectrophotometry. Our measurements indicated diameters a couple nanometers larger than specifications (a result of measurements on the fully hydrated state?), and remarkable monodispersity - within specs. It is clear to us now that our early immuno-gold results were dominated by the largest diameter spheres of our early colloids. We are delighted by the NanoPartz monodisperse preparations and recommend them strongly!"

Dr. Richard C. Haskell 
Professor of Physics & Director of Physics Clinic 
Physics Department 
Harvey Mudd College



"The scientists at Nanopartz have developed a line of colloidal gold nanorods with extremely high monodispersity in size, shape, and mass. The surfactant layer is highly stable and readily displaced for biofunctionalization. In every test, in vitro and in vivo, these materials have exceeded our expectations."


Geoffrey A. von Maltzahn
NSF and Whitaker Graduate Fellow
Harvard-MIT Division of Health Sciences and Technology
Laboratory for Multiscale Regenerative Technologies

 

Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres

A Malugin, H Ghandehari - Journal of Applied Toxicology, 2010 - Wiley Online Library
... Plain, PEGylated spherical and PEGylated rod GNPs were obtained from Nanopartz (Salt Lake ...
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. ...
4. TEM images of PC-3 cells incubated with plain spherical gold nanoparticles 30 nm ...

Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages

MM Janát-Amsbury, A Ray, CM Peterson… - European Journal of …, 2011 - Elsevier
... PEGylated gold nanoparticles (spherical particles of 50 nm in diameter and rod-shaped particles
with reported dimensions of 10 × 45 nm) were purchased from Nanopartz, Inc. (Loveland, CO,
USA). Before used, gold nanoparticle samples were incubated with cell culture ...

Computationally guided photothermal tumor therapy using long-circulating gold nanorod antennas

G von MaltzahnJH Park, A Agrawal, NK Bandaru… - Cancer research, 2009 - AACR
... coated gold NRs with longitudinal plasmon resonance at 810 nm (Nanopartz, a division of ... To
understand the relationship between the nanoparticle distribution in tumors and the ... to that
observed previously for PEG-protected 10-nm diameter spherical gold nanoparticles ( 21). ...

SERS‐coded gold nanorods as a multifunctional platform for densely multiplexed near‐infrared imaging and photothermal heating

G von Maltzahn, A Centrone, JH Park… - Advanced …, 2009 - Wiley Online Library
... IV), and DTDC-655 (VII) to display a 10–103 fold higher signal than three molecules with visible
absorbance shown previously to exhibit efficient near-infrared SERS on spherical gold nanopar-
ticles (I, II ... CTAB-coated gold NRs were purchased from Nanopartz with a ...

A simple millifluidic benchtop reactor system for the high-throughput synthesis and functionalization of gold nanoparticles with different sizes and shapes

SE Lohse, JR Eller, ST Sivapalan, MR Plews… - ACS …, 2013 - ACS Publications
... from several retailers in concentrations of >30 ppm (Sigma-Aldrich, Nanopartz, Nanoseedz, etc.). ...
for AuNRs in 2005 (which uses sodium borohydride, rather than spherical AuNP seeds ... batch
syntheses, even though the reactor permits synthesis of gold nanoparticles at higher ...

Negatively Charged Gold Nanoparticles Inhibit Alzheimer's Amyloid‐β Fibrillization, Induce Fibril Dissociation, and Mitigate Neurotoxicity

YH Liao, YJ Chang, Y Yoshiike, YC ChangYR Chen - Small, 2012 - Wiley Online Library
... In this research, we have examined the effects of gold nanoparticles (AuNPs) on amyloids ... to inhibit
Aβ fibrillization and redirect Aβ forming fragmented fibrils and spherical oligomers. ... Bare,
amine-conjugated, and carboxyl-conjugated AuNPs were purchased from Nanopartz Inc. ...

NanoPen: dynamic, low-power, and light-actuated patterning of nanoparticles

A Jamshidi, SL NealeK YuPJ Pauzauskie… - Nano …, 2009 - ACS Publications
... Figure 1c shows NanoPen immobilization and patterning of 90 nm diameter spherical gold
nanoparticles (purchased from Nanopartz Inc.(37 ... The stage is then manually transported leaving
a trace of gold nanoparticles in the illuminated area on the OET surface. ...

Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells

J Baumgart, L Humbert, É BoulaisR Lachaine… - Biomaterials, 2012 - Elsevier
... The cells were incubated during 3–6 h with the AuNP (8 μg/ml, diameter 100 nm, Nanopartz)
in the culture medium at 37 °C and 5 ... In summary, the use of off-resonance spherical gold
nanoparticles allows to reduce the energy absorption by the nanoparticle, inhibiting its ...

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN AfroozIA KhanSM Hussain… - … science & technology, 2013 - ACS Publications
... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs ... stabilized AuNSs with 10 nm primary particle diameter were procured from
Nanopartz Inc ... Part a of Figure 1 shows spherical structures with angular features for some ...

Influence of transient environmental photothermal effects on optical scattering by gold nanoparticles

EY Lukianova-HlebDO Lapotko - Nano letters, 2009 - ACS Publications
... 10-A-100, Nanopartz, Salt Lake City, UT) and 250 nm (no. ... figure Figure 1. Experimental setup:
individual gold nanoparticles are coaxially illuminated by a pump laser pulse (532 nm), by a
continuous probe laser (633 nm), and also by a probe laser pulse (690 nm). ...