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Nanopartz™ Plasmonic Fluorophores™

Nanopartz™ Plasmonic Fluorophores™ are fluorophore decorated gold nanorods manufactured using multiple patent pending and proprietary methods that result in fluorophores that exhibit much higher intensities than conventional fluorophores, no photobleaching even under the highest laser intensities, with chemical resistance to extreme pHs, salts, and other adverse chemicals. Peak fluorescence range from 550nm to 800nm, where each nanorod fluoresces up to 50,000 times brighter than a single fluorophore. Every batch is completely characterized including size, monodispersity, aggregation, and concentration. Residual chemicals are reduced to trace. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Conjugation options include amine, biotin, carboxyl, methyl, neutravidin, nhs, maleimide, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. These conjugated particles can be used in darkfield, fluorescence, and other imaging modes; ELISA, lateral flow, and other assays.

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Conjugated In-Vitro Plasmonic Fluorophores
Conjugated In-Vitro Plasmonic Fluorophores
Part #C16-(FLUOR)-(CONJUGATION)-(OD-mLs)
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $440.00

 

 

 

 

Part #  Diam (nm) Long Size (nm) Peak SPR Wave (nm) Ex/Em (nm) Wt. conc (mg/ml) (10-3) Wt. % PPM Molarity (pM) Spectrally Similar Dyes
C16-10-1064 10 67 1064 777/794 1750.0 0.1750% 1750 32.9 IRDye 800
C16-10-980 10 59 980 777/794 1750.0 0.1750% 1750 38.6 IRDye 800
C16-10-850 10 45 850 777/794 1750.0 0.1750% 1750 53.0 IRDye 800
C16-10-808 10 41 808 777/794 1750.0 0.1750% 1750 60.3 IRDye 800
C16-10-780 10 38 780 754/776 1750.0 0.1750% 1750 66.4 Alexa Fluor 750
C16-25-800 25 99 800 777/794 2500.0 0.2500% 2500 5.8 IRDye 800
C16-25-750 25 86 750 754/776 2500.0 0.2500% 2500 7.0 Alexa Fluor 750
C16-25-680 25 68 680 692/712 2500.0 0.2500% 2500 9.9 Alexa Fluor 680, Cy5.5
C16-25-650 25 60 650 652/672 2500.0 0.2500% 2500 12.0 Alexa Fluor 647, Cy5
C16-25-633 25 56 633 638/658 2500.0 0.2500% 2500 13.6 Alexa Fluor 633
C16-25-600 25 46 594 593/618 2500.0 0.2500% 2500 19.9 Alexa Fluor 594, Texas Red
C16-25-550 25 33 546 562/576 2500.0 0.2500% 2500 45.6 Alexa Fluor 546, Alexa Fluor 555

 

 

 

 

 

 

mutab

 

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

 

 

Fluorophore Type Excitation (nm) Emission (nm) Spectrally Similar Dyes
800 788 808 IRDye 800
750 750 773 Alexa Fluor 750
680 673 707 Alexa Fluor 680, Cy5.5
650 646 662 Alexa Fluor 647, Cy5
633 638 658 Alexa Fluor 633
600 591 604 Alexa Fluor 594, Texas Red
550 555 570 Alexa Fluor 546, Alexa Fluor 555
532 533 556  
514 520 540  
488 494 518 FITC, Alexa Fluor 488

 

 

 

Composition

These Plasmonic Fluorophores™ come in numerous conjugations:

Available in amine, biotin, carboxyl, methyl, neutravidin, streptavidin, nhs, maleimide, and azide forms. Methyl acts as a free acid form to be used as a non reactive negative control.

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM

Quantity

This product is available in 2.5mg and 12.5mg quantities. Buffer is PBS.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

Unknown. Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

Fluorescence (Nanopartz Flurophore I) for fluorescence measurements

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products. >

 

 

  Nanopartz™ Plasmonic Fluorophores Dye Labels
Cell Uptake Yes Difficult
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

  • Covalent bonds insure specificity, stability, long shelf life
  • Buffer Stability - stable from pH 4-9
  • No sodium azide
  • No BSA
  • Polymer coating insures no aggregation in high salts, reduced nonspecific binding
  • Stable
  • Well Characterized
  • Customer can select buffer
  • Customer can select gold nanoparticle type, size and/or SPR
  • Loading of all ligands is optimized
  • Customer can focus on research and not on Nanopartz expertise
  • Quick turnaround

microgold in vitro

Microgold (Part #C13-1000-TMU-5) with MUTAB coating under brightfield microscope, cancer cell, 100x objective

How much thickness does your proprietary capping agent add?

It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solvent you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

No

Will the SPR wavelength shift with different solvents?

The SPR is a function of the index of refraction of the host solvent in which the nanoparticles are suspended. Nanorods are the most sensitive and are therefore a great tool for measuring the index of refraction of the host.

What is the shelf life if I never open the package?

1 year.

Should I store in the refrigerator?

Yes.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

 

Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres

A Malugin, H Ghandehari - Journal of Applied Toxicology, 2010 - Wiley Online Library
... Plain, PEGylated spherical and PEGylated rod GNPs were obtained from Nanopartz (Salt Lake ...
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. ...
4. TEM images of PC-3 cells incubated with plain spherical gold nanoparticles 30 nm ...

Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages

MM Janát-Amsbury, A Ray, CM Peterson… - European Journal of …, 2011 - Elsevier
... PEGylated gold nanoparticles (spherical particles of 50 nm in diameter and rod-shaped particles
with reported dimensions of 10 × 45 nm) were purchased from Nanopartz, Inc. (Loveland, CO,
USA). Before used, gold nanoparticle samples were incubated with cell culture ...

Computationally guided photothermal tumor therapy using long-circulating gold nanorod antennas

G von MaltzahnJH Park, A Agrawal, NK Bandaru… - Cancer research, 2009 - AACR
... coated gold NRs with longitudinal plasmon resonance at 810 nm (Nanopartz, a division of ... To
understand the relationship between the nanoparticle distribution in tumors and the ... to that
observed previously for PEG-protected 10-nm diameter spherical gold nanoparticles ( 21). ...

SERS‐coded gold nanorods as a multifunctional platform for densely multiplexed near‐infrared imaging and photothermal heating

G von Maltzahn, A Centrone, JH Park… - Advanced …, 2009 - Wiley Online Library
... IV), and DTDC-655 (VII) to display a 10–103 fold higher signal than three molecules with visible
absorbance shown previously to exhibit efficient near-infrared SERS on spherical gold nanopar-
ticles (I, II ... CTAB-coated gold NRs were purchased from Nanopartz with a ...

A simple millifluidic benchtop reactor system for the high-throughput synthesis and functionalization of gold nanoparticles with different sizes and shapes

SE Lohse, JR Eller, ST Sivapalan, MR Plews… - ACS …, 2013 - ACS Publications
... from several retailers in concentrations of >30 ppm (Sigma-Aldrich, Nanopartz, Nanoseedz, etc.). ...
for AuNRs in 2005 (which uses sodium borohydride, rather than spherical AuNP seeds ... batch
syntheses, even though the reactor permits synthesis of gold nanoparticles at higher ...

Negatively Charged Gold Nanoparticles Inhibit Alzheimer's Amyloid‐β Fibrillization, Induce Fibril Dissociation, and Mitigate Neurotoxicity

YH Liao, YJ Chang, Y Yoshiike, YC ChangYR Chen - Small, 2012 - Wiley Online Library
... In this research, we have examined the effects of gold nanoparticles (AuNPs) on amyloids ... to inhibit
Aβ fibrillization and redirect Aβ forming fragmented fibrils and spherical oligomers. ... Bare,
amine-conjugated, and carboxyl-conjugated AuNPs were purchased from Nanopartz Inc. ...

NanoPen: dynamic, low-power, and light-actuated patterning of nanoparticles

A Jamshidi, SL NealeK YuPJ Pauzauskie… - Nano …, 2009 - ACS Publications
... Figure 1c shows NanoPen immobilization and patterning of 90 nm diameter spherical gold
nanoparticles (purchased from Nanopartz Inc.(37 ... The stage is then manually transported leaving
a trace of gold nanoparticles in the illuminated area on the OET surface. ...

Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells

J Baumgart, L Humbert, É BoulaisR Lachaine… - Biomaterials, 2012 - Elsevier
... The cells were incubated during 3–6 h with the AuNP (8 μg/ml, diameter 100 nm, Nanopartz)
in the culture medium at 37 °C and 5 ... In summary, the use of off-resonance spherical gold
nanoparticles allows to reduce the energy absorption by the nanoparticle, inhibiting its ...

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN AfroozIA KhanSM Hussain… - … science & technology, 2013 - ACS Publications
... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs ... stabilized AuNSs with 10 nm primary particle diameter were procured from
Nanopartz Inc ... Part a of Figure 1 shows spherical structures with angular features for some ...

Influence of transient environmental photothermal effects on optical scattering by gold nanoparticles

EY Lukianova-HlebDO Lapotko - Nano letters, 2009 - ACS Publications
... 10-A-100, Nanopartz, Salt Lake City, UT) and 250 nm (no. ... figure Figure 1. Experimental setup:
individual gold nanoparticles are coaxially illuminated by a pump laser pulse (532 nm), by a
continuous probe laser (633 nm), and also by a probe laser pulse (690 nm). ...