Spherical Gold UV VIS

Microgold (Part# C13-1000-MUTAB) with MUTAB coating under brightfield microscope, cancer cell, 100x objective. Inset shows individual microgold

Gold Nanorods UV VIS

Gold Nanowires (Part# C14-4000) under brightfield microscope, 40x objective

 

Nanopartz™ Gold Nanoparticles for in vitro Bright Field Imaging

Nanopartz™ Gold Nanoparticles for in vitro Bright Field Imaging utilize the complete line of microgold and nanowires for bright field microscopy. These conjugated particles exhibit true white light scattering - many orders of magnitude greater than quantum dot fluorescence. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. This product comes in one concentration, 0.25mg/mL. Ligands include our own proprietary MUTAB, providing a covalent, positive charged particles which has shown in literature to exhibit strong cellular uptake.

 

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Conjugated Microgold for In-Vitro
Conjugated Microgold for In-Vitro
C13-(LENGTH)-(CONJUGATION); 0.25mg in 1mL
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
Conjugated Gold Nanowires for In-Vitro
Conjugated Gold Nanowires for In-Vitro
C14-(LENGTH)-(CONJUGATION); 0.25mg in 1mL
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
Fluorophore Labeled Microgold
Fluorophore Labeled Microgold
Part #C16A3-(LENGTH)-(LABEL)-(CONJUGATION); 0.25mg in 1mL
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00
Fluorophore Labeled Gold Nanowires
Fluorophore Labeled Gold Nanowires
Part #C16A4-(LENGTH)-(LABEL)-(CONJUGATION); 0.25mg in 1mL
Sterilization
Certified Sterilized (+$150.00)
Endotoxin
Endotoxin Purified and Certified (+$250.00)
Negative Control
0.1mL Nonreactive Methyl (+$100.00)
1mL Nonreactive Methyl (+$480.00)
Price: $480.00

 

 

 

Part # Diameter (nm) Length (nm) Aspect Ratio Peak SPR Wave (nm) SPR OD (1cm) Microgold Vol (nm3) Microgold /mL Wt. conc (ug/ml) Wt. % PPM Molarity (pM) SPR Molar Ext. (M-1cm-1)
C13-500 75 500 6.7 510 0.25 2.10E+06 1.24E+09 50 0.01% 50 2.06 1.21E+08
C13-1000 100 1000 10 510 0.25 7.59E+06 3.42E+08 50 0.01% 50 0.57 4.39E+08

 

 

 

 

Part # Diameter (nm) Length (nm) Aspect Ratio Peak SPR Wave (nm) Nanowires Vol (nm3) Nanowires /mL Wt. conc (ug/ml) Wt. % PPM Molarity (pM) SPR Molar Ext. (M-1cm-1)
C14-1000 75 1000 13 510 4.31E+06 3.01E+09 250 0.03% 250 5.02 9.96E+06
C14-2000 75 2000 27 510 8.72E+06 1.49E+09 250 0.03% 250 2.48 2.02E+07
C14-4000 75 4000 53 510 1.76E+07 7.39E+08 250 0.03% 250 1.23 4.06E+07
C14-6000 75 6000 80 510 2.64E+07 4.91E+08 250 0.03% 250 0.82 6.10E+07
C14-10000 75 10000 133 510 4.40E+07 2.94E+08 250 0.03% 250 0.49 1.02E+08

 

 

 

 

Functionalized Nanorod Block Invitro

mutab

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer (top) and Nanopartz™ covalent MUTAB (bottom). Thickness of polymer is less than the size variablity of the gold nanoparticle.

 

Conjugations (Ordering Abbreviation)

Amine TA
Biotin TB
Carboxyl TC
Maleimide TMAL
Methyl TM
Neutravidin TN
NHS TNHS
Streptavidin TS
MUTAB TMU
Secondary antibodies  

 

 

Composition

These in vitro gold nanoparticles for in vitro Bright field imaging come in numerous conjugations:

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

  1. Amine
  2. Biotin
  3. Carboxyl
  4. Methyl
  5. Neutravidin
  6. Streptavidin
  7. Azide
  8. MUTAB (a strongly positive non cytotoxic CTAB replacement)

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

  1. Goat Anti-Human IgG
  2. Goat Anti-Mouse IgG
  3. Goat Anti-Rabitt IgG
  4. Goat Anti-Human IgA
  5. Goat Anti-Human IgM
  6. Protein A

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

These gold nanoparticles exhibit white light scattering in brightfield microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

 

 

  Nanopartz™ Gold Nanoparticles for BrightField Imaging Citrate spherical gold nanoparticles with adsorbed conjugates
Stability (salt,pH,chemicals) High Low
Scattering linewidth Narrow (monodisperse) Broad (polydisperse)
Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm)
Nonspecific binding Very low High
pH and salt resistance High Medium
Sterilization Radiation Sodium Azide
Site specific antibody conjugation Yes No

 

 

 

  • Extremely stable. Shelf life is greater than six months.
  • Covalent bonds.
  • High anisotropy.
  • Low cytotoxicity. Gold is FDA approved for rheumatoid arthritis.
  • Excellent photostability
  • Buffer Stability - stable from pH 4-9.
  • Instrument capability - any brightfield microscope

Spherical Gold UV VIS

Microgold (Part# C13-1000-MUTAB) with MUTAB coating under brightfield microscope, cancer cell, 100x objective. Inset shows individual microgold

Gold Nanorods UV VIS

Gold Nanowires (Part# C14-4000) under brightfield microscope, 40x objective

 

 

 

"We were previously making our own gold nanoparticles using the commonly used technique of citrate reduction of Au(III). The colloids generated are broadly polydisperse, and we were concerned that our application of these particles in immuno-gold labeling of cells for optical coherence microscopy (OCM) would be utilizing only the large diameter tail of the distribution. We purchased the sampler package of NanoPartz gold spheres (30 nm, 50 nm, 70 nm, 90 nm) and examined each size carefully with dynamic light scattering, OCM, and spectrophotometry. Our measurements indicated diameters a couple nanometers larger than specifications (a result of measurements on the fully hydrated state?), and remarkable monodispersity - within specs. It is clear to us now that our early immuno-gold results were dominated by the largest diameter spheres of our early colloids. We are delighted by the NanoPartz monodisperse preparations and recommend them strongly!"

Dr. Richard C. Haskell 
Professor of Physics & Director of Physics Clinic 
Physics Department 
Harvey Mudd College



"The scientists at Nanopartz have developed a line of colloidal gold nanorods with extremely high monodispersity in size, shape, and mass. The surfactant layer is highly stable and readily displaced for biofunctionalization. In every test, in vitro and in vivo, these materials have exceeded our expectations."


Geoffrey A. von Maltzahn
NSF and Whitaker Graduate Fellow
Harvard-MIT Division of Health Sciences and Technology
Laboratory for Multiscale Regenerative Technologies

 

Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres

A Malugin, H Ghandehari - Journal of Applied Toxicology, 2010 - Wiley Online Library
... Plain, PEGylated spherical and PEGylated rod GNPs were obtained from Nanopartz (Salt Lake ...
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. ...
4. TEM images of PC-3 cells incubated with plain spherical gold nanoparticles 30 nm ...

Geometry and surface characteristics of gold nanoparticles influence their biodistribution and uptake by macrophages

MM Janát-Amsbury, A Ray, CM Peterson… - European Journal of …, 2011 - Elsevier
... PEGylated gold nanoparticles (spherical particles of 50 nm in diameter and rod-shaped particles
with reported dimensions of 10 × 45 nm) were purchased from Nanopartz, Inc. (Loveland, CO,
USA). Before used, gold nanoparticle samples were incubated with cell culture ...

Computationally guided photothermal tumor therapy using long-circulating gold nanorod antennas

G von MaltzahnJH Park, A Agrawal, NK Bandaru… - Cancer research, 2009 - AACR
... coated gold NRs with longitudinal plasmon resonance at 810 nm (Nanopartz, a division of ... To
understand the relationship between the nanoparticle distribution in tumors and the ... to that
observed previously for PEG-protected 10-nm diameter spherical gold nanoparticles ( 21). ...

SERS‐coded gold nanorods as a multifunctional platform for densely multiplexed near‐infrared imaging and photothermal heating

G von Maltzahn, A Centrone, JH Park… - Advanced …, 2009 - Wiley Online Library
... IV), and DTDC-655 (VII) to display a 10–103 fold higher signal than three molecules with visible
absorbance shown previously to exhibit efficient near-infrared SERS on spherical gold nanopar-
ticles (I, II ... CTAB-coated gold NRs were purchased from Nanopartz with a ...

A simple millifluidic benchtop reactor system for the high-throughput synthesis and functionalization of gold nanoparticles with different sizes and shapes

SE Lohse, JR Eller, ST Sivapalan, MR Plews… - ACS …, 2013 - ACS Publications
... from several retailers in concentrations of >30 ppm (Sigma-Aldrich, Nanopartz, Nanoseedz, etc.). ...
for AuNRs in 2005 (which uses sodium borohydride, rather than spherical AuNP seeds ... batch
syntheses, even though the reactor permits synthesis of gold nanoparticles at higher ...

Negatively Charged Gold Nanoparticles Inhibit Alzheimer's Amyloid‐β Fibrillization, Induce Fibril Dissociation, and Mitigate Neurotoxicity

YH Liao, YJ Chang, Y Yoshiike, YC ChangYR Chen - Small, 2012 - Wiley Online Library
... In this research, we have examined the effects of gold nanoparticles (AuNPs) on amyloids ... to inhibit
Aβ fibrillization and redirect Aβ forming fragmented fibrils and spherical oligomers. ... Bare,
amine-conjugated, and carboxyl-conjugated AuNPs were purchased from Nanopartz Inc. ...

NanoPen: dynamic, low-power, and light-actuated patterning of nanoparticles

A Jamshidi, SL NealeK YuPJ Pauzauskie… - Nano …, 2009 - ACS Publications
... Figure 1c shows NanoPen immobilization and patterning of 90 nm diameter spherical gold
nanoparticles (purchased from Nanopartz Inc.(37 ... The stage is then manually transported leaving
a trace of gold nanoparticles in the illuminated area on the OET surface. ...

Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells

J Baumgart, L Humbert, É BoulaisR Lachaine… - Biomaterials, 2012 - Elsevier
... The cells were incubated during 3–6 h with the AuNP (8 μg/ml, diameter 100 nm, Nanopartz)
in the culture medium at 37 °C and 5 ... In summary, the use of off-resonance spherical gold
nanoparticles allows to reduce the energy absorption by the nanoparticle, inhibiting its ...

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN AfroozIA KhanSM Hussain… - … science & technology, 2013 - ACS Publications
... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs ... stabilized AuNSs with 10 nm primary particle diameter were procured from
Nanopartz Inc ... Part a of Figure 1 shows spherical structures with angular features for some ...

Influence of transient environmental photothermal effects on optical scattering by gold nanoparticles

EY Lukianova-HlebDO Lapotko - Nano letters, 2009 - ACS Publications
... 10-A-100, Nanopartz, Salt Lake City, UT) and 250 nm (no. ... figure Figure 1. Experimental setup:
individual gold nanoparticles are coaxially illuminated by a pump laser pulse (532 nm), by a
continuous probe laser (633 nm), and also by a probe laser pulse (690 nm). ...