Nanopartz^TM Conjugation Kits are instructional guides to help educate customers in the methods of attaching their antibodies, dna, and other ligands to our gold nanoparticles using polymer covalent bonds.  Nanopartz^TM  also offer custom conjugation services.  Go to Custom Conjugations for more information.  Click on the image buttons to the right for more information.

Kits

There are three kits available, all relying on using standard EDC chemistry and carboxyl bonds to bind to primary amines.  The first kit comes complete with carboxyl-polymer conjugated to a customer chosen spherical gold nanoparticle, EDC chemicals, and instructions to conjugate an included IgG to our gold nanoparticles.  This kit includes discussions on theory, background, purification, and other important principles involved.  The second kit is for the more advanced user.  This one includes the EDC chemicals and gold nanoparticles for use in conjugations involving user supplied antibodies or dna.  It does not include instructions or IgG - it is recommended that the customer purchase Kit 1 first.  Kit 3 is for the most experienced user that has already purchased our gold nanoparticles in 250 OD-mL volumes and need the EDC chemicals for their conjugation.

Part number 21-PC-KIT1

This is a kit to instruct the conjugation of carboxyl-polymer spherical gold nanoparticles (SGNPs) to any primary amine using EDC chemistry.  This kit includes IgG as the conjugation target.  The customer specifies the gold nanoparticle size desired (5-100nm). 

EDC is a carboxyl and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond and release of an isourea by-product (see the Additional Information Section). The intermediate is unstable in aqueous solutions, and therefore, two step conjugation procedures require N-hydroxysuccinimide for stabilization. Failure to react with an amine will result in  hydrolysis of the intermediate, regeneration of the carboxyl and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

Part number 21-PC-KIT2

This kit provides all the chemicals necessary to perform EDC chemistry on our carboxyl-polymer spheres.  This is the same as KIT1 but without the IgG.  It is targeted to those that are experienced in KIT1 but now want to attach our gold nanoparticles to their own antibodies or other target with a primary amine.  Both our KIT1 and KIT2 are for 50 OD-mLs of gold nanoparticles.

Part number 21-PC-KIT3

This kit provides all the chemicals necessary to conjugate 250 OD-mLs of gold nanoparticles.  Unlike KIT2, it does not contain gold nanoparticles. 

Custom Formulation

These kits are based on EDC chemistry activating carboxyl terminated polymer conjugations so as to attach to any primary amine. 

Delivery

All kits are in stock and are ready to ship in 1-3 days.  All shipments are sent Fed Ex Standard Overnight delivery.  No shipments on Fridays.   

 

Diameter (nm)	Peak SPR Wavelength (nm)	NPS/ml	Wt. conc (mg/ml)	Wt. %	PPM	Molarity (pM)	Molar Ext. (M-1cm-1)	Absorption Molar Ext. (M-1cm-1)	Scattering Molar Ext. (M-1cm-1)	Size Dispersity %CV
5	514-520	2.1E+15	2322	0.23%	2322	3492600	2.86E+05	2.86E+05	0.00E+00	<20%
10	514-520	2.7E+14	2369	0.24%	2369	445440	2.24E+06	2.24E+06	0.00E+00	<20%
20	514-520	3.4E+13	2417	0.24%	2417	56811	1.76E+07	1.76E+07	0.00E+00	<10%
30	523	1.0E+13	2446	0.24%	2446	17032	5.87E+07	5.87E+07	0.00E+00	<6%
40	527	4.3E+12	2466	0.25%	2466	7246	1.38E+08	1.38E+08	4.00E+05	<4%
50	531	2.2E+12	2482	0.25%	2482	3734	2.68E+08	2.57E+08	1.04E+07	<4%
60	536	1.3E+12	2496	0.25%	2496	2172	4.60E+08	4.17E+08	4.37E+07	<4%
70	542	8.2E+11	2507	0.25%	2507	1374	7.28E+08	6.03E+08	1.24E+08	<4%
80	549	5.5E+11	2517	0.25%	2517	924	1.08E+09	7.93E+08	2.89E+08	<4%
90	558	3.9E+11	2525	0.25%	2525	651	1.54E+09	9.48E+08	5.88E+08	<4%
100	569	2.9E+11	2533	0.25%	2533	476	2.10E+09	1.01E+09	1.09E+09	<4%