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Conjugated Spherical Gold

NanopartzTM Accurate Conjugated Spherical Gold Nanoparticles

NanopartzTM AccurateTM brand conjugated spherical gold nanoparticles are manufactured using our proprietary and patent pending method that results in exact sizes, and highly monodisperse sizes and shapes, and adds the added advantage of proprietary polymer caged coating with carboxyl, methyl, amine, biotin, or neutravidin functional groups.  These particles are perfect for customer conjugation to most antibodies and DNA for all in vivo applications.  NanopartzTM conjugated gold nanoparticles are the only nanoparticles in the industry that come with a Certificate of Analysis assuring conjugation These conjugations have some of the longest shelf lives in the industry - up to six months.

 

Composition


Patented gold colloid with a proprietary polymer caged conjugation with methyl, carboxyl, amine, biotin or neutravidin active groups resulting in a patent pending composition. Proprietary charge control to reduce non-specific binding.   Excess active groups are reduced to less than 0.1% the concentration of the gold nanoparticles.

 

  

Specifications

 

Diameter (nm) Peak SPR Wavelength (nm) NPS/ml Wt. conc (mg/ml) Molarity (pM) Molar Ext. (M-1cm-1) Absorption Molar Ext. (M-1cm-1) Scattering Molar Ext. (M-1cm-1) Size Dispersity %CV
5 514-520 2.1E+15 2322 3492600 2.86E+05 2.86E+05 0.00E+00 <20%
10 514-520 2.7E+14 2369 445440 2.24E+06 2.24E+06 0.00E+00 <20%
15 514-520 8.0E+13 2397 133543 7.49E+06 7.49E+06 0.00E+00 <20%
20 514-520 3.4E+13 2417 56811 1.76E+07 1.76E+07 0.00E+00 <10%
25 514-520 1.8E+13 2433 29276 3.42E+07 3.46E+07 -4.65E+05 <10%
30 523 1.0E+13 2446 17032 5.87E+07 5.95E+07 -7.69E+05 <7%
35 526 6.5E+12 2457 10774 9.28E+07 9.35E+07 -7.05E+05 <7%
40 527 4.3E+12 2466 7246 1.38E+08 1.38E+08 4.00E+05 <7%
45 529 3.1E+12 2475 5106 1.96E+08 1.92E+08 3.60E+06 <7%
50 531 2.2E+12 2482 3734 2.68E+08 2.57E+08 1.04E+07 <7%
55 533 1.7E+12 2489 2813 3.55E+08 3.33E+08 2.29E+07 <7%
60 536 1.3E+12 2496 2172 4.60E+08 4.17E+08 4.37E+07 <7%
65 539 1.0E+12 2501 1712 5.84E+08 5.08E+08 7.61E+07 <7%
70 542 8.2E+11 2507 1374 7.28E+08 6.03E+08 1.24E+08 <7%
75 545 6.7E+11 2512 1119 8.93E+08 7.00E+08 1.93E+08 <4%
80 549 5.5E+11 2517 924 1.08E+09 7.93E+08 2.89E+08 <4%
85 553 4.6E+11 2521 772 1.30E+09 8.78E+08 4.18E+08 <4%
90 558 3.9E+11 2525 651 1.54E+09 9.48E+08 5.88E+08 <4%
95 563 3.3E+11 2529 555 1.80E+09 9.95E+08 8.09E+08 <4%
100 569 2.9E+11 2533 476 2.10E+09 1.01E+09 1.09E+09 <4%

 

*                   Shape monodispersity (% spheres) > 99.9%.

*                   Size accuracy +/- 2 nm.

*                   See Tech Note TN801 for definition of terms and method of analysis.

*                   All solutions are optical density (OD) = 50.

*                   These are typical values.  Exact values are measured at the time of manufacturing.

 

 

 

Zeta Potential

 

Conjugation

Charge (mV)

Methyl

0

Carboxyl

-20

Amine

 10

Neutravidin

-0.5

 

 Coverage

 

Sphere Dia (nm) # Active sites/particle
5 1
10 2
20 10
30 20
40 30
50 50
60 70
70 100
80 125
90 150
100 200

  

 

Custom Formulation and Production

 

Quantity

  • Neutravidin, methyl, carboxyl, amine, and biotin volumes of 1 mL and 5 mL, OD 50 (concentration is 50x our bare gold nanoparticles), or 50 and 250 OD-mLs, respectively.

  • For larger quantities, or for orders amounting over 50 mL per annum, please contact sales for quantity pricing.

Delivery

  • Shipped sealed in low retention micro-centrifuge containers.

  • All are stored in water at pH=7, except for carboxyl and neutravidin which are stored in DI water plus a dilute buffer and and surfactant.

  • Orders come with exact size and concentration calculation, along with a Certificate of Analysis with size, concentration, zeta charge, and peak SPR.

  • Most conjugations ship in 1 week.

  • All shipments are made Fed Ex Standard Overnight delivery. International orders shipped Fedex Priority 2 day.  No shipments on Fridays.

Part #s

 

      PN# 20-P(A,C,M,N)-SSS-OD

  • -P(A,C,M,N) is conjugation; Methyl -PM; Carboxyl -PC; Amine -PA;  Neutravidin -PN

  • -SSS is nanoparticle size; 5-100 nm

  • -OD is OD-mLs; This product ships in volumes of 1 mL and 5 mL at OD 50 which amounts to 50 and 250 OD-mLs, respectively.

  • Example: Accurate Conjugated SGNPs, Carboxyl, 30 nm, 50 OD-mL would be: 20-PC-30-50

Price

  • Methyl, Carboxyl, Amine and Biotin 50 OD-mL $440; 250 OD-mL $1760

  • Neutravidin 50 OD-mL $480; 250 OD-mL $1920

  • Each product comes highly concentrated, 50 times greater than our regular bare gold nanoparticles.  We do this for a number of reasons detailed in our FAQz.  Further, our yield is about 50% so it takes over 100 mL of gold nanoparticles to make 1 mL of concentrated conjugated particles. 

 

 

 Ordering Information

 

 

Conjugated Spherical Gold Part Numbers (add -OD for OD-mLs at the end of each part number as described above)

 

Sphere Diameter (nm) Methyl Carboxyl Amine Neutravidin
5 20-PM-5 20-PC-5 20-PA-5 20-PN-5
10 20-PM-10 20-PC-10 20-PA-10 20-PN-10
20 20-PM-20 20-PC-20 20-PA-20 20-PN-20
30 20-PM-30 20-PC-30 20-PA-30 20-PN-30
40 20-PM-40 20-PC-40 20-PA-40 20-PN-40
50 20-PM-50 20-PC-50 20-PA-50 20-PN-50
60 20-PM-60 20-PC-60 20-PA-60 20-PN-60
70 20-PM-70 20-PC-70 20-PA-70 20-PN-70
80 20-PM-80 20-PC-80 20-PA-80 20-PN-80
90 20-PM-90 20-PC-90 20-PA-90 20-PN-90
100 20-PM-100 20-PC-100 20-PA-100 20-PN-100

 

 

Applications

Conjugated spherical gold nanoparticles are important in:

  • Custom conjugations using antibodies or DNA

  • Homogeneous and heterogeneous Plasmon Resonance Labels

  • Dark Field Microscopy

  • Cellular uptake

  • Fluorescence quenching

  • X-Ray Contrast enhancement


 

 
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Technical Resources

 

Frequently Asked Questions (FAQz)

 

Storage and Shelf Life
 

Ordering Information

 

Pricing and ordering Info

 

Related Technical Notes

 

Conjugated Gold MSDS

 

Info Note IN801 The benefits of Neutravidin over Streptavidin

 

Info Note IN804 UV-VIS Nomenclature and Units

 

Tech Note TN801 UV-VIS to determine size and concentration of SGNPs

 

Tech Note TN804 Introduction to Dark Field Microscopy